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The map label for this gene is folC [H]

Identifier: 59712301

GI number: 59712301

Start: 1906256

End: 1907536

Strand: Reverse

Name: folC [H]

Synonym: VF_1694

Alternate gene names: 59712301

Gene position: 1907536-1906256 (Counterclockwise)

Preceding gene: 59712302

Following gene: 59712300

Centisome position: 65.83

GC content: 38.64

Gene sequence:

>1281_bases
ATGACAACATTTGATTCTCCGAAAGCCACATCTGATTTATCGGTGTGGCTTCGTTATTTAGAAAGCATTCATACTAGTGC
AATAGATTTGGGTTTAGACCGTGTTCAGCGTGTTGCACAACGAGCAAAATTAACTAAACCAGCACCTACGGTAATTACTG
TTGCAGGAACAAACGGTAAAGGCTCAACGTGTGCATTAATGGAATCTATTTTAATTAATGCCGGCTACCGCGTTGGTGTT
TATAGTTCTCCTCACCTTATTCGTTACAATGAACGTATTCGTATTAATGGTAACGATTTATCTGATTCTCTTATCACTCA
ATCTTTTGCTGCAATTGAAGCGTGGCGTAAAGATATTTCACTTAGCCTTTTCGAATTTGGAACTCTTTCAGCTCTTTGGG
CTTTTAAAGCACATCAAGTTGACGTGGTTTTACTTGAAGTTGGTCTTGGTGGACGCTTAGATGCAACTAATGTTGTTGAT
CATGATGTATCAATTATTACAAGCCTAGCCGTTGATCACGTTGATTGGTTGGGTGATGATATTGAAGTGATTGGTTTTGA
AAAGGCTGGCATTTTCCGTTCAGAAAAACCAGCTATTTGTGGTCAACCTAATCCTCCTTCAAGTGTCGCTGCTCATGCTG
ATGATATTAATGCTGAACTATATCAAGTTGGTTATCAATTTGATTATAAAGTCGATGCAGAAACTCAAACATGGTCATGG
AAGAGTGGAGCATTTGATTTAGATAATTTACCATTACCAAATCTACCATTAGCTAATGCAGCAACAGCGATTATGGCATT
AGGGGTTTCTGGATTAGAATTGAGTGATGTTAATATTGTTGAAGGCCTAACATTAGCAACGTTACCAGGAAGAATGCAAC
GATTAACTTGTGGTAACTCTGAACTTGAGCCTACGATTATTCTTGATGTTGCGCATAACCCACATTCAGCGCACTATCTT
GTACAGCAAGTAAGACAGCGTTTCCCAAATAAGAAAATACATGCGGTAGTAGGTATGTTGCATGATAAAGACATTAAATC
GACCCTGAGTGAGTTCACGGGAGTGATTGATGTTTGGTATCCTGCATCGTTATCAGGTCCAAGAGCGGCTAACGCAAGTG
AGTTAACTGCTGTTTTATCAAAAGAAATAACAGAATATTCATCACCTGTTGATGCTTTTAAAGTAGCATTACAGCAGGCT
GAAAATGATGATTTAATTTTAGTCTTCGGATCATTCCACACTGTCGGTGAAGTATGGGATTATTGGAAAAAAGAAGCATG
A

Upstream 100 bases:

>100_bases
GAATTAAATCAAATGGGACCAAGGTTGTTAGTTCTTACTTTAAAACAGCAACCTAAGTCATATATACTGTCACTATATTC
GTTAGTGAAGTAGTTGAATT

Downstream 100 bases:

>100_bases
GGAATATGAGTGTCTAGTCCATTTCAACGACGTTTAGTCGGAACCATAATATTGGTTGCTATTGGGGTAATTGTTTTACC
TGATGTATTTGATGGTAAGA

Product: bifunctional folylpolyglutamate synthase/ dihydrofolate synthase

Products: NA

Alternate protein names: Folylpolyglutamate synthase; Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolylpolyglutamate synthase; Tetrahydrofolate synthase; Dihydrofolate synthase [H]

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MTTFDSPKATSDLSVWLRYLESIHTSAIDLGLDRVQRVAQRAKLTKPAPTVITVAGTNGKGSTCALMESILINAGYRVGV
YSSPHLIRYNERIRINGNDLSDSLITQSFAAIEAWRKDISLSLFEFGTLSALWAFKAHQVDVVLLEVGLGGRLDATNVVD
HDVSIITSLAVDHVDWLGDDIEVIGFEKAGIFRSEKPAICGQPNPPSSVAAHADDINAELYQVGYQFDYKVDAETQTWSW
KSGAFDLDNLPLPNLPLANAATAIMALGVSGLELSDVNIVEGLTLATLPGRMQRLTCGNSELEPTIILDVAHNPHSAHYL
VQQVRQRFPNKKIHAVVGMLHDKDIKSTLSEFTGVIDVWYPASLSGPRAANASELTAVLSKEITEYSSPVDAFKVALQQA
ENDDLILVFGSFHTVGEVWDYWKKEA

Sequences:

>Translated_426_residues
MTTFDSPKATSDLSVWLRYLESIHTSAIDLGLDRVQRVAQRAKLTKPAPTVITVAGTNGKGSTCALMESILINAGYRVGV
YSSPHLIRYNERIRINGNDLSDSLITQSFAAIEAWRKDISLSLFEFGTLSALWAFKAHQVDVVLLEVGLGGRLDATNVVD
HDVSIITSLAVDHVDWLGDDIEVIGFEKAGIFRSEKPAICGQPNPPSSVAAHADDINAELYQVGYQFDYKVDAETQTWSW
KSGAFDLDNLPLPNLPLANAATAIMALGVSGLELSDVNIVEGLTLATLPGRMQRLTCGNSELEPTIILDVAHNPHSAHYL
VQQVRQRFPNKKIHAVVGMLHDKDIKSTLSEFTGVIDVWYPASLSGPRAANASELTAVLSKEITEYSSPVDAFKVALQQA
ENDDLILVFGSFHTVGEVWDYWKKEA
>Mature_425_residues
TTFDSPKATSDLSVWLRYLESIHTSAIDLGLDRVQRVAQRAKLTKPAPTVITVAGTNGKGSTCALMESILINAGYRVGVY
SSPHLIRYNERIRINGNDLSDSLITQSFAAIEAWRKDISLSLFEFGTLSALWAFKAHQVDVVLLEVGLGGRLDATNVVDH
DVSIITSLAVDHVDWLGDDIEVIGFEKAGIFRSEKPAICGQPNPPSSVAAHADDINAELYQVGYQFDYKVDAETQTWSWK
SGAFDLDNLPLPNLPLANAATAIMALGVSGLELSDVNIVEGLTLATLPGRMQRLTCGNSELEPTIILDVAHNPHSAHYLV
QQVRQRFPNKKIHAVVGMLHDKDIKSTLSEFTGVIDVWYPASLSGPRAANASELTAVLSKEITEYSSPVDAFKVALQQAE
NDDLILVFGSFHTVGEVWDYWKKEA

Specific function: Conversion of folates to polyglutamate derivatives [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=186, Percent_Identity=41.3978494623656, Blast_Score=128, Evalue=9e-30,
Organism=Homo sapiens, GI66932990, Length=186, Percent_Identity=41.3978494623656, Blast_Score=128, Evalue=1e-29,
Organism=Escherichia coli, GI1788654, Length=420, Percent_Identity=52.6190476190476, Blast_Score=414, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI17553148, Length=474, Percent_Identity=28.4810126582279, Blast_Score=134, Evalue=7e-32,
Organism=Caenorhabditis elegans, GI17553150, Length=468, Percent_Identity=28.6324786324786, Blast_Score=134, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI71984923, Length=474, Percent_Identity=28.4810126582279, Blast_Score=134, Evalue=8e-32,
Organism=Saccharomyces cerevisiae, GI6323760, Length=348, Percent_Identity=31.0344827586207, Blast_Score=129, Evalue=6e-31,
Organism=Saccharomyces cerevisiae, GI6324815, Length=182, Percent_Identity=36.2637362637363, Blast_Score=105, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24641571, Length=297, Percent_Identity=33.6700336700337, Blast_Score=124, Evalue=9e-29,
Organism=Drosophila melanogaster, GI24581568, Length=189, Percent_Identity=38.6243386243386, Blast_Score=104, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17; =6.3.2.12 [H]

Molecular weight: Translated: 46559; Mature: 46428

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1 ; PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTFDSPKATSDLSVWLRYLESIHTSAIDLGLDRVQRVAQRAKLTKPAPTVITVAGTNGK
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCC
GSTCALMESILINAGYRVGVYSSPHLIRYNERIRINGNDLSDSLITQSFAAIEAWRKDIS
CCHHHHHHHHHHCCCCEEEECCCCCEEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHCC
LSLFEFGTLSALWAFKAHQVDVVLLEVGLGGRLDATNVVDHDVSIITSLAVDHVDWLGDD
EEEHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
IEVIGFEKAGIFRSEKPAICGQPNPPSSVAAHADDINAELYQVGYQFDYKVDAETQTWSW
EEEEEEECCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCEEEEECEEEEEEECCCCCEECC
KSGAFDLDNLPLPNLPLANAATAIMALGVSGLELSDVNIVEGLTLATLPGRMQRLTCGNS
CCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEECCCHHHCCCEEEECCCHHHEEECCCC
ELEPTIILDVAHNPHSAHYLVQQVRQRFPNKKIHAVVGMLHDKDIKSTLSEFTGVIDVWY
CCCCEEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHCCEEEEEE
PASLSGPRAANASELTAVLSKEITEYSSPVDAFKVALQQAENDDLILVFGSFHTVGEVWD
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEEECCHHHHHHHH
YWKKEA
HHHCCC
>Mature Secondary Structure 
TTFDSPKATSDLSVWLRYLESIHTSAIDLGLDRVQRVAQRAKLTKPAPTVITVAGTNGK
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCC
GSTCALMESILINAGYRVGVYSSPHLIRYNERIRINGNDLSDSLITQSFAAIEAWRKDIS
CCHHHHHHHHHHCCCCEEEECCCCCEEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHCC
LSLFEFGTLSALWAFKAHQVDVVLLEVGLGGRLDATNVVDHDVSIITSLAVDHVDWLGDD
EEEHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
IEVIGFEKAGIFRSEKPAICGQPNPPSSVAAHADDINAELYQVGYQFDYKVDAETQTWSW
EEEEEEECCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCEEEEECEEEEEEECCCCCEECC
KSGAFDLDNLPLPNLPLANAATAIMALGVSGLELSDVNIVEGLTLATLPGRMQRLTCGNS
CCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEECCCHHHCCCEEEECCCHHHEEECCCC
ELEPTIILDVAHNPHSAHYLVQQVRQRFPNKKIHAVVGMLHDKDIKSTLSEFTGVIDVWY
CCCCEEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHCCEEEEEE
PASLSGPRAANASELTAVLSKEITEYSSPVDAFKVALQQAENDDLILVFGSFHTVGEVWD
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEEECCHHHHHHHH
YWKKEA
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3040739; 3040734; 9205837; 9278503; 1989505 [H]