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The map label for this gene is kefA [H]

Identifier: 59712263

GI number: 59712263

Start: 1867982

End: 1868863

Strand: Direct

Name: kefA [H]

Synonym: VF_1656

Alternate gene names: 59712263

Gene position: 1867982-1868863 (Clockwise)

Preceding gene: 59712262

Following gene: 59712265

Centisome position: 64.47

GC content: 41.04

Gene sequence:

>882_bases
ATGATTCGTTTATTTGTACTTTGCTTATCGCTGATGATGAGCTCAACTGCATTGGCTGAAGATGCTCAATCAATCGATAA
TTTACAACAATTTGCCAGTTTAATCCGTTGGAGTGGTGTTGCAGCCTCCTTCTTTATTATCCTAGGTGCGTGGTTATTCT
TACGTTTTATTCAATCAATTGTCGATGGGGTAAGTAGCCAATTTGCTCAGCGCCGTATGTTGATGCAAAAGCTGCAATCG
TTCTTTCAATTCTTTATTTACATGTCGACAGCCGTTGCGGTTTTCATGTTGAGTTTTCGAGTTGATGATCGAGTCTTAGC
TTTAATTGGTGGTACGATTGCGGTATCTGTGGGTTTTGCCTTAAAAGACCTTACCGCCTCTTTCATCGCAGGCTTAACCG
TAATGATTGATCGTCCATTCCAAGTGGGTGACCGGGTGACGTTTGAAGGCCACTACGGTGACATTATCGCCATTGGTCTT
CGCTCTGTTCGCATGCAAACCTTGAACGACGATATTATTACCATTCCAAATAACAAATTCTTAAATGAAGTCACCGTCAG
TGGTAACTACGGTGGGTTGGATATGCAGGTGGTGATCCCTTTCTATATTGGAATGGATCAAGACATCAATAAAGCACGAG
ATATCATCCAAGAAGCGGCGTCTTCAAGTCGTTATATTCACTTACCAAAACCGGTTGTGGTATTAGTAAAGCAGACGATT
ACCGATAACTATTTGGCGATTCAACTGACCTGTAAAGCTTATGTGGTAGATATTAAGTTTGAAAAACTCTTTGAAACAGA
CATTACGTTACGTGTGATGCAAGAGTTTAGGAAAGCCAATATCATTCCACCAACCATCGCAATACAGAGCAAATTAGCGT
AA

Upstream 100 bases:

>100_bases
GGTTACATTGAATGGAATGATGAAAAAGCACGTATATCAGACCACTGGTATCGTCATATTACTAACGTATTACATCGTCA
ACACTTACTGGTGAAATAAC

Downstream 100 bases:

>100_bases
TAGAAAATAAAAAAGGTAGAGTTATTAGGCTCTACCTTTTTTTATTGGATGAGGATCAGAACTAAATTACGCCTAACTCT
CGTAAACGCTCCATCAAATA

Product: hypothetical protein

Products: NA

Alternate protein names: Protein AefA [H]

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MIRLFVLCLSLMMSSTALAEDAQSIDNLQQFASLIRWSGVAASFFIILGAWLFLRFIQSIVDGVSSQFAQRRMLMQKLQS
FFQFFIYMSTAVAVFMLSFRVDDRVLALIGGTIAVSVGFALKDLTASFIAGLTVMIDRPFQVGDRVTFEGHYGDIIAIGL
RSVRMQTLNDDIITIPNNKFLNEVTVSGNYGGLDMQVVIPFYIGMDQDINKARDIIQEAASSSRYIHLPKPVVVLVKQTI
TDNYLAIQLTCKAYVVDIKFEKLFETDITLRVMQEFRKANIIPPTIAIQSKLA

Sequences:

>Translated_293_residues
MIRLFVLCLSLMMSSTALAEDAQSIDNLQQFASLIRWSGVAASFFIILGAWLFLRFIQSIVDGVSSQFAQRRMLMQKLQS
FFQFFIYMSTAVAVFMLSFRVDDRVLALIGGTIAVSVGFALKDLTASFIAGLTVMIDRPFQVGDRVTFEGHYGDIIAIGL
RSVRMQTLNDDIITIPNNKFLNEVTVSGNYGGLDMQVVIPFYIGMDQDINKARDIIQEAASSSRYIHLPKPVVVLVKQTI
TDNYLAIQLTCKAYVVDIKFEKLFETDITLRVMQEFRKANIIPPTIAIQSKLA
>Mature_293_residues
MIRLFVLCLSLMMSSTALAEDAQSIDNLQQFASLIRWSGVAASFFIILGAWLFLRFIQSIVDGVSSQFAQRRMLMQKLQS
FFQFFIYMSTAVAVFMLSFRVDDRVLALIGGTIAVSVGFALKDLTASFIAGLTVMIDRPFQVGDRVTFEGHYGDIIAIGL
RSVRMQTLNDDIITIPNNKFLNEVTVSGNYGGLDMQVVIPFYIGMDQDINKARDIIQEAASSSRYIHLPKPVVVLVKQTI
TDNYLAIQLTCKAYVVDIKFEKLFETDITLRVMQEFRKANIIPPTIAIQSKLA

Specific function: Mechanosensitive channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell, although it does not appear to have a major role in osmolarity regulation. Form

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786670, Length=186, Percent_Identity=24.7311827956989, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 32855; Mature: 32855

Theoretical pI: Translated: 8.19; Mature: 8.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRLFVLCLSLMMSSTALAEDAQSIDNLQQFASLIRWSGVAASFFIILGAWLFLRFIQSI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
VDGVSSQFAQRRMLMQKLQSFFQFFIYMSTAVAVFMLSFRVDDRVLALIGGTIAVSVGFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHH
LKDLTASFIAGLTVMIDRPFQVGDRVTFEGHYGDIIAIGLRSVRMQTLNDDIITIPNNKF
HHHHHHHHHCCHHEEEECCCCCCCEEEECCCCCCEEEEHHHHHHHHHCCCCEEECCCCCC
LNEVTVSGNYGGLDMQVVIPFYIGMDQDINKARDIIQEAASSSRYIHLPKPVVVLVKQTI
CCEEEECCCCCCEEEEEEEEEHCCCCCCHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHH
TDNYLAIQLTCKAYVVDIKFEKLFETDITLRVMQEFRKANIIPPTIAIQSKLA
CCCEEEEEEEEEEEEEEEEHHHHHHCCHHHHHHHHHHHCCCCCCCHHHCCCCC
>Mature Secondary Structure
MIRLFVLCLSLMMSSTALAEDAQSIDNLQQFASLIRWSGVAASFFIILGAWLFLRFIQSI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
VDGVSSQFAQRRMLMQKLQSFFQFFIYMSTAVAVFMLSFRVDDRVLALIGGTIAVSVGFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHH
LKDLTASFIAGLTVMIDRPFQVGDRVTFEGHYGDIIAIGLRSVRMQTLNDDIITIPNNKF
HHHHHHHHHCCHHEEEECCCCCCCEEEECCCCCCEEEEHHHHHHHHHCCCCEEECCCCCC
LNEVTVSGNYGGLDMQVVIPFYIGMDQDINKARDIIQEAASSSRYIHLPKPVVVLVKQTI
CCEEEECCCCCCEEEEEEEEEHCCCCCCHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHH
TDNYLAIQLTCKAYVVDIKFEKLFETDITLRVMQEFRKANIIPPTIAIQSKLA
CCCEEEEEEEEEEEEEEEEHHHHHHCCHHHHHHHHHHHCCCCCCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9278503; 10202137 [H]