Click here to switch to the map view.

The map label for this gene is hisM [H]

Identifier: 59712192

GI number: 59712192

Start: 1776121

End: 1776798

Strand: Reverse

Name: hisM [H]

Synonym: VF_1585

Alternate gene names: 59712192

Gene position: 1776798-1776121 (Counterclockwise)

Preceding gene: 59712193

Following gene: 59712189

Centisome position: 61.32

GC content: 35.69

Gene sequence:

>678_bases
ATGGATTTTTCAATTATCATTGAGAGCTTTCCTGTTTATCTTGAAGGCTTATGGACAACGGTGTGGTTAGTAGCGGTATC
TTTGGTGATTGGTTTGTGTATTGCTGTACCAACAGCGATAGCAAGAAACAGCAATAACCCAGTTTTATGGTTACCAGCTT
GGGCATACATTTACTTTTTTCGTGGTACGCCTTTACTGATTCAACTGTATCTTATTTATTATGGTATGGATCAATTTTTC
TCTGTTAAAGATACCTTATGGGAACACGCTTCATTTTGTGCTCTTGTTGCTTTTTCTTTAAATACAGGAGCTTATACAGC
TGAAATTGTACGTGGTTCTATTAATGGTTTACCACAAGGAGAAGTTGAAGCTGCCAAAGCGTATGGTATGAATCAATTCC
AAACGTATAAACGAATTATTTTACCTAGTGCGTTACGTAGAGCATTACCAGCTTATAGTAATGAAGTGATTTTCATGCTT
CATGGTTCAGCCATTGCCGGGATTGTTACTTTACTGGATTTAACTGGTGCTGCACGATTAGTAAATTCAAGATATTACGC
ACCATTTGAATCCTATATTGCTGCCGGTATGTTTTATATGTGCTTAACATTTATTATTATTTTTATATTTAAGCAATTGG
AAACTCGCATGCTCAGATTTATGATGCCATTAAGTTAA

Upstream 100 bases:

>100_bases
TTTATATGACAGTTGCTTGTATTTTCTTATTTTTCACAGCAGTTTCAACTGGTGCGTTAAAACTTATTGAACGTAAATTT
AGTATTCACATGAGGTAGAT

Downstream 100 bases:

>100_bases
TTAGATTATTTACAGTCAAAAATTCCAACACAGTTATCGAGTATATAAACAAACTAGGATAACCAGTTAGTTACGACGAT
TGGTATTAATATCCATTTAG

Product: histidine/lysine/arginine/ornithine transporter subunit

Products: ADP; phosphate; L-histidine [Cytoplasm]; ADP; L-ornithine [Cytoplasm]; L-arginine [Cytoplasm]; L-lysine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MDFSIIIESFPVYLEGLWTTVWLVAVSLVIGLCIAVPTAIARNSNNPVLWLPAWAYIYFFRGTPLLIQLYLIYYGMDQFF
SVKDTLWEHASFCALVAFSLNTGAYTAEIVRGSINGLPQGEVEAAKAYGMNQFQTYKRIILPSALRRALPAYSNEVIFML
HGSAIAGIVTLLDLTGAARLVNSRYYAPFESYIAAGMFYMCLTFIIIFIFKQLETRMLRFMMPLS

Sequences:

>Translated_225_residues
MDFSIIIESFPVYLEGLWTTVWLVAVSLVIGLCIAVPTAIARNSNNPVLWLPAWAYIYFFRGTPLLIQLYLIYYGMDQFF
SVKDTLWEHASFCALVAFSLNTGAYTAEIVRGSINGLPQGEVEAAKAYGMNQFQTYKRIILPSALRRALPAYSNEVIFML
HGSAIAGIVTLLDLTGAARLVNSRYYAPFESYIAAGMFYMCLTFIIIFIFKQLETRMLRFMMPLS
>Mature_225_residues
MDFSIIIESFPVYLEGLWTTVWLVAVSLVIGLCIAVPTAIARNSNNPVLWLPAWAYIYFFRGTPLLIQLYLIYYGMDQFF
SVKDTLWEHASFCALVAFSLNTGAYTAEIVRGSINGLPQGEVEAAKAYGMNQFQTYKRIILPSALRRALPAYSNEVIFML
HGSAIAGIVTLLDLTGAARLVNSRYYAPFESYIAAGMFYMCLTFIIIFIFKQLETRMLRFMMPLS

Specific function: Component of the nopaline active transport system probably consisting of four subunits:Q, M, P and T. This system is also capable of transporting octopine provided that catabolic functions are induced with nopaline [H]

COG id: COG4160

COG function: function code E; ABC-type arginine/histidine transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788645, Length=215, Percent_Identity=45.1162790697674, Blast_Score=180, Evalue=6e-47,
Organism=Escherichia coli, GI1787086, Length=218, Percent_Identity=34.8623853211009, Blast_Score=130, Evalue=5e-32,
Organism=Escherichia coli, GI1788226, Length=219, Percent_Identity=35.6164383561644, Blast_Score=123, Evalue=8e-30,
Organism=Escherichia coli, GI1787030, Length=216, Percent_Identity=30.5555555555556, Blast_Score=111, Evalue=4e-26,
Organism=Escherichia coli, GI1787087, Length=221, Percent_Identity=26.2443438914027, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1786873, Length=219, Percent_Identity=31.0502283105023, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1788646, Length=211, Percent_Identity=28.436018957346, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI1786874, Length=171, Percent_Identity=27.4853801169591, Blast_Score=77, Evalue=7e-16,
Organism=Escherichia coli, GI87082239, Length=204, Percent_Identity=27.4509803921569, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010065
- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 25402; Mature: 25402

Theoretical pI: Translated: 8.02; Mature: 8.02

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFSIIIESFPVYLEGLWTTVWLVAVSLVIGLCIAVPTAIARNSNNPVLWLPAWAYIYFF
CCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHH
RGTPLLIQLYLIYYGMDQFFSVKDTLWEHASFCALVAFSLNTGAYTAEIVRGSINGLPQG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCC
EVEAAKAYGMNQFQTYKRIILPSALRRALPAYSNEVIFMLHGSAIAGIVTLLDLTGAARL
HHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHH
VNSRYYAPFESYIAAGMFYMCLTFIIIFIFKQLETRMLRFMMPLS
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDFSIIIESFPVYLEGLWTTVWLVAVSLVIGLCIAVPTAIARNSNNPVLWLPAWAYIYFF
CCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHH
RGTPLLIQLYLIYYGMDQFFSVKDTLWEHASFCALVAFSLNTGAYTAEIVRGSINGLPQG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCC
EVEAAKAYGMNQFQTYKRIILPSALRRALPAYSNEVIFMLHGSAIAGIVTLLDLTGAARL
HHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHH
VNSRYYAPFESYIAAGMFYMCLTFIIIFIFKQLETRMLRFMMPLS
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-histidine [Periplasm]; H2O; ATP; L-ornithine [Periplasm]; L-arginine [Periplasm]; L-lysine [Periplasm] [C]

Specific reaction: ATP + L-histidine [Periplasm] + H2O = ADP + phosphate + L-histidine [Cytoplasm] ATP + L-ornithine [Periplasm] + H2O = ADP + phosphate + L-ornithine [Cytoplasm] ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] ATP + L-lysine [P

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1732218; 11743193; 11743194 [H]