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The map label for this gene is nrfE [H]

Identifier: 59712157

GI number: 59712157

Start: 1733089

End: 1734975

Strand: Reverse

Name: nrfE [H]

Synonym: VF_1550

Alternate gene names: 59712157

Gene position: 1734975-1733089 (Counterclockwise)

Preceding gene: 59712158

Following gene: 59712156

Centisome position: 59.88

GC content: 37.04

Gene sequence:

>1887_bases
ATGATTGCTGAAATTGGACAGTTTTGGTTAATAACCACTATGGTGTTGTCTGCTGTTGCTTGTGTGACAGCAAGTTACGG
TATTTTTAGAAATGAAGACAATTGGGCCGTATCTATTTTCCCACTTTCATTATTAACAGCTCTTTTTTGTATTTTATCCA
TATCTGCTTTAGGTTATGGGTTTTATATTGATGACTTTTCTATTCGATATATAGCCGAGCACTCTAATTCTGCATTACCT
GTATTTTTTAAAATTGCTGCGATTTGGGGTGGGCATGAAGGTTCATTACTATTTTGGGTATTAACACTCACCTTATGGGT
TGGCTTAATTACATTAAAAAGAAAAAGCATTGAGCAATCTTATTTAGTACGTGTTCTTTTAATATTAAATGGATTAATTG
TTATCTTTTCAGCCTTTACTTTATTTGCATCTAATCCTTTTATTATTTATGCCAATACGCCTTTAGAAGGACGAGATTTG
AATCCAATGTTACAAGATATTGGATTGATATTTCATCCCCCATTGTTATATCTCGGTTATGTTGGTTTTGCTGCTACCTT
TTCATTTGCACTCGCTGCATTAATGATGAAAAAAGTGCCATCTCAATGGGTCGGCTATGCGAGAAACTGGACATTGCTTG
CTTGGTCATTGCTTACTGCTGGGATCAGTCTTGGCTCATGGTGGGCCTATTATGAGTTAGGTTGGGGTGGATGGTGGTTT
TGGGACCCAGTAGAAAACGCTTCTTTGTTGCCTTGGTTAACATCCACCGCATTACTACACACATTAATTGCTAGTCATAA
GAGAAAGCTATTACTAAAAGCATCGATTATTTTGGCATTAGTGACTTTTTGTTTGAGTATCTTAGGGACTTTTGTGGTTC
GCTCAGGGGTATTAACGTCGGTTCATGCGTTTGCTGTCGATCCAACAAGAGGCATCATATTATTACTAATTTTGTTTATT
GTAATGTTGCTCTCTTTTAGCCTTTATACAGTAAAAAGCAGACAAATTAACAGTGAACCCATCCAACATTTTATCAGTCG
TGATTTTCTATTTTTAGTGTTGTGTGGCTTATTCGCAATTGCAACGGTGACGGTATTACTTGGCACGTTTTATCCTATGA
TTTTTCAGATCTTAGGGTTAGGGAATATTTCGGTAGGGGCACCTTATTTTAATTTACTTTTTGTTCCAATGGCGCTTATT
GCACTCGGTATGATGGCGTTATCGATTTTTGTGCAATGGAATAAGAAAGCAGAGATGGCAACAATAAAAAATACAGCCAT
ATTCATTGGTAGTTCAGTTATTAGTGGAATGCTGATTTATACACTTCAATATGATTGTATTTACGTTATTCCTCTTATTG
TGTGGATCTTATCTATTGCCGTTATTATTGGAACGGTGTGGGAAATAAAACGATCTAAAGGTAAAAATATAAGCATGGCA
ATCGCGCATATTGGTGTGGCTGTGGTTATGATTGGCGCTGCGATGAACAGTTATCATTCATTTGAAACCAGTGCAAAAAT
GGAACCTGGAATGAGTGCGAAACTTGCGAATTATAATATCCATTATAAAGATACTGAGCTATTAGTTGGTGCTAATTATA
CAGCAGAGCAAGCGAATTTACGTATTTATGAAAATGGGAAAGAAATAGCGAATGTTCACCCTCAACGTCGACATTATCAA
GTACGAGTGATGAACATGAGTGAGCCGGCAATGCACTGGTTTTGGCGCGGCGATATATACGTTACACTGGGCGAAAAATT
AAAAGATGGAAGTTTTGCGTTACGATTGCAATATAAAGCGTACGTGAGGTGGATTTGGTTTGGCTCATTGTTAATGTGTG
TTGGAGGGGTATTCGCTATGGTTAAAAGAAAGAGAAAAACAGCATGA

Upstream 100 bases:

>100_bases
ATGCAGGACAAATGACGGTCGTATAATGTAGTAATGCCATGCTTATGCCAATAATGACGAGCATAAGCATGGCTTTTATG
TGAAAAATGTATGGAGCAAT

Downstream 100 bases:

>100_bases
AGCAAATAGCTAAGGTTCTCACTGCTATCATTTTGTGTATTGCAGTTTTTTTTGTGTTACTAAGTGGTTTAGAAACCAAG
GAATCCTCAGGTTCTACAGC

Product: heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 628; Mature: 628

Protein sequence:

>628_residues
MIAEIGQFWLITTMVLSAVACVTASYGIFRNEDNWAVSIFPLSLLTALFCILSISALGYGFYIDDFSIRYIAEHSNSALP
VFFKIAAIWGGHEGSLLFWVLTLTLWVGLITLKRKSIEQSYLVRVLLILNGLIVIFSAFTLFASNPFIIYANTPLEGRDL
NPMLQDIGLIFHPPLLYLGYVGFAATFSFALAALMMKKVPSQWVGYARNWTLLAWSLLTAGISLGSWWAYYELGWGGWWF
WDPVENASLLPWLTSTALLHTLIASHKRKLLLKASIILALVTFCLSILGTFVVRSGVLTSVHAFAVDPTRGIILLLILFI
VMLLSFSLYTVKSRQINSEPIQHFISRDFLFLVLCGLFAIATVTVLLGTFYPMIFQILGLGNISVGAPYFNLLFVPMALI
ALGMMALSIFVQWNKKAEMATIKNTAIFIGSSVISGMLIYTLQYDCIYVIPLIVWILSIAVIIGTVWEIKRSKGKNISMA
IAHIGVAVVMIGAAMNSYHSFETSAKMEPGMSAKLANYNIHYKDTELLVGANYTAEQANLRIYENGKEIANVHPQRRHYQ
VRVMNMSEPAMHWFWRGDIYVTLGEKLKDGSFALRLQYKAYVRWIWFGSLLMCVGGVFAMVKRKRKTA

Sequences:

>Translated_628_residues
MIAEIGQFWLITTMVLSAVACVTASYGIFRNEDNWAVSIFPLSLLTALFCILSISALGYGFYIDDFSIRYIAEHSNSALP
VFFKIAAIWGGHEGSLLFWVLTLTLWVGLITLKRKSIEQSYLVRVLLILNGLIVIFSAFTLFASNPFIIYANTPLEGRDL
NPMLQDIGLIFHPPLLYLGYVGFAATFSFALAALMMKKVPSQWVGYARNWTLLAWSLLTAGISLGSWWAYYELGWGGWWF
WDPVENASLLPWLTSTALLHTLIASHKRKLLLKASIILALVTFCLSILGTFVVRSGVLTSVHAFAVDPTRGIILLLILFI
VMLLSFSLYTVKSRQINSEPIQHFISRDFLFLVLCGLFAIATVTVLLGTFYPMIFQILGLGNISVGAPYFNLLFVPMALI
ALGMMALSIFVQWNKKAEMATIKNTAIFIGSSVISGMLIYTLQYDCIYVIPLIVWILSIAVIIGTVWEIKRSKGKNISMA
IAHIGVAVVMIGAAMNSYHSFETSAKMEPGMSAKLANYNIHYKDTELLVGANYTAEQANLRIYENGKEIANVHPQRRHYQ
VRVMNMSEPAMHWFWRGDIYVTLGEKLKDGSFALRLQYKAYVRWIWFGSLLMCVGGVFAMVKRKRKTA
>Mature_628_residues
MIAEIGQFWLITTMVLSAVACVTASYGIFRNEDNWAVSIFPLSLLTALFCILSISALGYGFYIDDFSIRYIAEHSNSALP
VFFKIAAIWGGHEGSLLFWVLTLTLWVGLITLKRKSIEQSYLVRVLLILNGLIVIFSAFTLFASNPFIIYANTPLEGRDL
NPMLQDIGLIFHPPLLYLGYVGFAATFSFALAALMMKKVPSQWVGYARNWTLLAWSLLTAGISLGSWWAYYELGWGGWWF
WDPVENASLLPWLTSTALLHTLIASHKRKLLLKASIILALVTFCLSILGTFVVRSGVLTSVHAFAVDPTRGIILLLILFI
VMLLSFSLYTVKSRQINSEPIQHFISRDFLFLVLCGLFAIATVTVLLGTFYPMIFQILGLGNISVGAPYFNLLFVPMALI
ALGMMALSIFVQWNKKAEMATIKNTAIFIGSSVISGMLIYTLQYDCIYVIPLIVWILSIAVIIGTVWEIKRSKGKNISMA
IAHIGVAVVMIGAAMNSYHSFETSAKMEPGMSAKLANYNIHYKDTELLVGANYTAEQANLRIYENGKEIANVHPQRRHYQ
VRVMNMSEPAMHWFWRGDIYVTLGEKLKDGSFALRLQYKAYVRWIWFGSLLMCVGGVFAMVKRKRKTA

Specific function: Required for the biogenesis of c-type cytochromes. Possible subunit of a heme lyase [H]

COG id: COG1138

COG function: function code O; Cytochrome c biogenesis factor

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ccmF/cycK/ccl1/nrfE/ccsA family [H]

Homologues:

Organism=Escherichia coli, GI1788524, Length=645, Percent_Identity=38.7596899224806, Blast_Score=426, Evalue=1e-120,
Organism=Escherichia coli, GI1790511, Length=572, Percent_Identity=38.6363636363636, Blast_Score=351, Evalue=8e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002541
- InterPro:   IPR003567
- InterPro:   IPR003568
- InterPro:   IPR003570
- InterPro:   IPR017563 [H]

Pfam domain/function: PF01578 Cytochrom_C_asm [H]

EC number: NA

Molecular weight: Translated: 70421; Mature: 70421

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAEIGQFWLITTMVLSAVACVTASYGIFRNEDNWAVSIFPLSLLTALFCILSISALGYG
CCCCHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCEEEEEHHHHHHHHHHHHHHHHHCCE
FYIDDFSIRYIAEHSNSALPVFFKIAAIWGGHEGSLLFWVLTLTLWVGLITLKRKSIEQS
EEEECCEEEEEEECCCCCCHHHHEEEHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
YLVRVLLILNGLIVIFSAFTLFASNPFIIYANTPLEGRDLNPMLQDIGLIFHPPLLYLGY
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHCCHHHCCHHHHHHH
VGFAATFSFALAALMMKKVPSQWVGYARNWTLLAWSLLTAGISLGSWWAYYELGWGGWWF
HHHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCEEE
WDPVENASLLPWLTSTALLHTLIASHKRKLLLKASIILALVTFCLSILGTFVVRSGVLTS
ECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
VHAFAVDPTRGIILLLILFIVMLLSFSLYTVKSRQINSEPIQHFISRDFLFLVLCGLFAI
HHHEEECCCHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ATVTVLLGTFYPMIFQILGLGNISVGAPYFNLLFVPMALIALGMMALSIFVQWNKKAEMA
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCE
TIKNTAIFIGSSVISGMLIYTLQYDCIYVIPLIVWILSIAVIIGTVWEIKRSKGKNISMA
EECCHHEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
IAHIGVAVVMIGAAMNSYHSFETSAKMEPGMSAKLANYNIHYKDTELLVGANYTAEQANL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCEEEEECCEEEEECCCCCCCCCE
RIYENGKEIANVHPQRRHYQVRVMNMSEPAMHWFWRGDIYVTLGEKLKDGSFALRLQYKA
EEEECCCHHHCCCCCCCEEEEEEEECCCCHHEEEEECCEEEEECCCCCCCCEEEEEEHHH
YVRWIWFGSLLMCVGGVFAMVKRKRKTA
HHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIAEIGQFWLITTMVLSAVACVTASYGIFRNEDNWAVSIFPLSLLTALFCILSISALGYG
CCCCHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCEEEEEHHHHHHHHHHHHHHHHHCCE
FYIDDFSIRYIAEHSNSALPVFFKIAAIWGGHEGSLLFWVLTLTLWVGLITLKRKSIEQS
EEEECCEEEEEEECCCCCCHHHHEEEHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
YLVRVLLILNGLIVIFSAFTLFASNPFIIYANTPLEGRDLNPMLQDIGLIFHPPLLYLGY
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHCCHHHCCHHHHHHH
VGFAATFSFALAALMMKKVPSQWVGYARNWTLLAWSLLTAGISLGSWWAYYELGWGGWWF
HHHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCEEE
WDPVENASLLPWLTSTALLHTLIASHKRKLLLKASIILALVTFCLSILGTFVVRSGVLTS
ECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
VHAFAVDPTRGIILLLILFIVMLLSFSLYTVKSRQINSEPIQHFISRDFLFLVLCGLFAI
HHHEEECCCHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ATVTVLLGTFYPMIFQILGLGNISVGAPYFNLLFVPMALIALGMMALSIFVQWNKKAEMA
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCE
TIKNTAIFIGSSVISGMLIYTLQYDCIYVIPLIVWILSIAVIIGTVWEIKRSKGKNISMA
EECCHHEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
IAHIGVAVVMIGAAMNSYHSFETSAKMEPGMSAKLANYNIHYKDTELLVGANYTAEQANL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCEEEEECCEEEEECCCCCCCCCE
RIYENGKEIANVHPQRRHYQVRVMNMSEPAMHWFWRGDIYVTLGEKLKDGSFALRLQYKA
EEEECCCHHHCCCCCCCEEEEEEEECCCCHHEEEEECCEEEEECCCCCCCCEEEEEEHHH
YVRWIWFGSLLMCVGGVFAMVKRKRKTA
HHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]