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The map label for this gene is nrfD [H]

Identifier: 59712158

GI number: 59712158

Start: 1735050

End: 1736006

Strand: Reverse

Name: nrfD [H]

Synonym: VF_1551

Alternate gene names: 59712158

Gene position: 1736006-1735050 (Counterclockwise)

Preceding gene: 59712159

Following gene: 59712157

Centisome position: 59.91

GC content: 39.18

Gene sequence:

>957_bases
ATGAGTGCATGGGAAGCAGCGTTTAATTTCGACTCTTTAGTATGGGATTGGATCATTGCGATCTACCTTTTCTTAGCCGG
TATGTCTGCTGGTGCGGTTATGATATCGATTTACTTAAAACGTAAAGTGATTGAAGGTAACCCTGCAGATAACGGTATTA
TCAAAGCAACCGCAATTTTAGCGCCATTTGGCATTATTGCAGGTTTAACCATTTTGATTTTCCACTTAACCAAACCACTC
TCTTTTTGGAAAATTATGATTTTCTTTAACCCAACATCCGTGATGTCGATGGGGGTTATTCTATTCCAAGTGTATATGGC
TGTGCTATTTGCTTGGCTTGGCGTAATGTTTAGAAAGGATATCATGAGCTTTCTAAAAGGAAAGTTTGGTTTTATCGATA
CTATCTTGCTTAAGATTGAACAGTTTGAAAATGCGTTAGAAATCTTTTTAGCTGTGCTTGCTATTATGCTTGCGGCTTAT
ACTGGTTTCTTATTGTCAGCATTACAAACGTATCCAATGTTAAATAACCCGGTATTGCCGATTTTATTCTTGTTCTCAAG
TTTATCGTCTGGTGCAGCGGCGTGTTTACTGTTTGGTATTTTAGGTTTTAAAGAACCAAGCCATAGCCATTCGGTGAAGT
GGATCCATAACTTTGAACGACCTGTTGTACTGTTTGAGTTGTTCGTACTTGTTACCTTCTTTACTGGTTTAATCTTTAGT
GGAGGGCAGGGCGAAGCTGCTGCTTGGGCTGCTATTGGTGGTGGTTTTTGGGGAATATGGTTCTGGGTTGGCGTTATTGG
TTTAGGTATGATCACCCCTTTAACTTTGAATGCATTTGCGCCAAAGGAATTGGTTCACGGTAAAGGGTTTATTTTTGTTG
TGACATCATTAAGTTTACTTGGTGTATTAATGCTTCGTACGTTTGTGTTGTATGCAGGACAAATGACGGTCGTATAA

Upstream 100 bases:

>100_bases
AATGCGGTATTGAATAGTAACCCAGTATACCGTGACAAAGTGGATCTAGGTACCAAGCCTAAATTGTATAAGATTCCATA
TTTAAAAGGGGAGATTTAAG

Downstream 100 bases:

>100_bases
TGTAGTAATGCCATGCTTATGCCAATAATGACGAGCATAAGCATGGCTTTTATGTGAAAAATGTATGGAGCAATATGATT
GCTGAAATTGGACAGTTTTG

Product: formate-dependent nitrite reductase, membrane subunit

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MSAWEAAFNFDSLVWDWIIAIYLFLAGMSAGAVMISIYLKRKVIEGNPADNGIIKATAILAPFGIIAGLTILIFHLTKPL
SFWKIMIFFNPTSVMSMGVILFQVYMAVLFAWLGVMFRKDIMSFLKGKFGFIDTILLKIEQFENALEIFLAVLAIMLAAY
TGFLLSALQTYPMLNNPVLPILFLFSSLSSGAAACLLFGILGFKEPSHSHSVKWIHNFERPVVLFELFVLVTFFTGLIFS
GGQGEAAAWAAIGGGFWGIWFWVGVIGLGMITPLTLNAFAPKELVHGKGFIFVVTSLSLLGVLMLRTFVLYAGQMTVV

Sequences:

>Translated_318_residues
MSAWEAAFNFDSLVWDWIIAIYLFLAGMSAGAVMISIYLKRKVIEGNPADNGIIKATAILAPFGIIAGLTILIFHLTKPL
SFWKIMIFFNPTSVMSMGVILFQVYMAVLFAWLGVMFRKDIMSFLKGKFGFIDTILLKIEQFENALEIFLAVLAIMLAAY
TGFLLSALQTYPMLNNPVLPILFLFSSLSSGAAACLLFGILGFKEPSHSHSVKWIHNFERPVVLFELFVLVTFFTGLIFS
GGQGEAAAWAAIGGGFWGIWFWVGVIGLGMITPLTLNAFAPKELVHGKGFIFVVTSLSLLGVLMLRTFVLYAGQMTVV
>Mature_317_residues
SAWEAAFNFDSLVWDWIIAIYLFLAGMSAGAVMISIYLKRKVIEGNPADNGIIKATAILAPFGIIAGLTILIFHLTKPLS
FWKIMIFFNPTSVMSMGVILFQVYMAVLFAWLGVMFRKDIMSFLKGKFGFIDTILLKIEQFENALEIFLAVLAIMLAAYT
GFLLSALQTYPMLNNPVLPILFLFSSLSSGAAACLLFGILGFKEPSHSHSVKWIHNFERPVVLFELFVLVTFFTGLIFSG
GQGEAAAWAAIGGGFWGIWFWVGVIGLGMITPLTLNAFAPKELVHGKGFIFVVTSLSLLGVLMLRTFVLYAGQMTVV

Specific function: Probably involved in the transfer of electrons from the quinone pool to the type-c cytochromes [H]

COG id: COG3301

COG function: function code P; Formate-dependent nitrite reductase, membrane component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nrfD family [H]

Homologues:

Organism=Escherichia coli, GI1790509, Length=315, Percent_Identity=47.6190476190476, Blast_Score=280, Evalue=1e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017566
- InterPro:   IPR005614 [H]

Pfam domain/function: PF03916 NrfD [H]

EC number: NA

Molecular weight: Translated: 35030; Mature: 34898

Theoretical pI: Translated: 8.95; Mature: 8.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAWEAAFNFDSLVWDWIIAIYLFLAGMSAGAVMISIYLKRKVIEGNPADNGIIKATAIL
CCCHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
APFGIIAGLTILIFHLTKPLSFWKIMIFFNPTSVMSMGVILFQVYMAVLFAWLGVMFRKD
HHHHHHHHHHHHHHHHHCCHHHHEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IMSFLKGKFGFIDTILLKIEQFENALEIFLAVLAIMLAAYTGFLLSALQTYPMLNNPVLP
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
ILFLFSSLSSGAAACLLFGILGFKEPSHSHSVKWIHNFERPVVLFELFVLVTFFTGLIFS
HHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
GGQGEAAAWAAIGGGFWGIWFWVGVIGLGMITPLTLNAFAPKELVHGKGFIFVVTSLSLL
CCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHH
GVLMLRTFVLYAGQMTVV
HHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SAWEAAFNFDSLVWDWIIAIYLFLAGMSAGAVMISIYLKRKVIEGNPADNGIIKATAIL
CCHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH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HHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8057835; 8265357; 9278503 [H]