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The map label for this gene is iadA [H]

Identifier: 59712116

GI number: 59712116

Start: 1682357

End: 1683493

Strand: Reverse

Name: iadA [H]

Synonym: VF_1509

Alternate gene names: 59712116

Gene position: 1683493-1682357 (Counterclockwise)

Preceding gene: 59712118

Following gene: 59712115

Centisome position: 58.1

GC content: 37.55

Gene sequence:

>1137_bases
ATGTTTATTTTATTAAAGAATGCAAATGTATACGCTCCTCAGCACTTAGGAAAAGTTAATGTGTTGATTGGTTGTGGAAA
GGTAATTTCTATTGAATCTGATTTGAATGTTCAAGGCATTGGTGATATTTCGATTATTGATTGTAAAGGGAAAACAGTCA
CTCCAGGCTTAATAGATCAACATGTACATCTGATTGGAGGAGGTGGTGAGGGAGGTTTTTCTAGTCGAACACCACAAGTT
ACATTTTCTAAGTTAATTCAAGCAGGAACAACAAGTGTCGTTGGGGTATTGGGAACAGATGGCATTTCTCGTTCACCACG
CGATTTATATGCAAAAGCTGCAGCACTTACAGAAGAAGGGATTACAGCTTATATGCATACTGGGTCGTATGAGGTGCCAA
CAAAAACGATAACTACATCCATTCGGGATGATATCTCTTTTTTACCTTCAGTTCTCGGTGTAAAGATAGCACTAGCAGAT
CATAGGTGCTCATTTCCAACGTTACAAGAATTGAGTCGTATTGTTTCCGATATACGTATTGCTAGCTTATTAGCTAAAAA
ACGAGGGGTGTTGCACATGCATATGGGGGCTCTATCTGATCCTTTTGCTTCTGTTTTTGAGTTATTAAATATGGGGATAC
CCATTAGCCATTTTTCACCAACCCATGTTGCTCGAACTGAATCTTTATTTAGTGAAGCTATGCAATTTGCTCGCCGAGGT
GGTTATATTGATATCACATCTGGAGGTAGTCAATTTATTGCCCCTGAGCTTGCGATTAAACAAGCGATAGAATCTGGTAT
AGATCCGAAACATATAACAGTAAGTTCAGATGGGAATGGTAGTCTGCCAAAGTTTGATATTGATGGAAATATGGTTGCGT
TAACTGCTGCAAATGTTGATGGAAACTTATTGTTATTACCTAAATTGATTGAATCAGGTATTAGCCCCGAAATAGCTATT
GCAATGTTAACATCAAATGTTGCAGATTCTCTGGGCATTAATAAAGGTCGATTACAGCTTGGTCAAGAAGCTGATTTATG
CATATTTAACGATGACTTTACACTTTATGGGGTAGTAGCAAAAGGTAAAACAGTACTGCTTGATAATGAATTACTTGTAA
CTGGAAACTTTGAATAA

Upstream 100 bases:

>100_bases
TTATTGTTTTGATAGTTCTAGTTCTCAAATATTCATCGAGATAAATATCAGGATATAAGAGAGGTGGAATGTATAAATAA
TATATGAAGTAGAGGGAATG

Downstream 100 bases:

>100_bases
GGGATTAGATATGAGTTTATTTATTGCATTATGTGGGTTGATTCTTGCGGGAAGATTAATTTTAAAAAACTATAACCCCC
AAGGCGTACTCTTCTTTGTT

Product: isoaspartyl dipeptidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MFILLKNANVYAPQHLGKVNVLIGCGKVISIESDLNVQGIGDISIIDCKGKTVTPGLIDQHVHLIGGGGEGGFSSRTPQV
TFSKLIQAGTTSVVGVLGTDGISRSPRDLYAKAAALTEEGITAYMHTGSYEVPTKTITTSIRDDISFLPSVLGVKIALAD
HRCSFPTLQELSRIVSDIRIASLLAKKRGVLHMHMGALSDPFASVFELLNMGIPISHFSPTHVARTESLFSEAMQFARRG
GYIDITSGGSQFIAPELAIKQAIESGIDPKHITVSSDGNGSLPKFDIDGNMVALTAANVDGNLLLLPKLIESGISPEIAI
AMLTSNVADSLGINKGRLQLGQEADLCIFNDDFTLYGVVAKGKTVLLDNELLVTGNFE

Sequences:

>Translated_378_residues
MFILLKNANVYAPQHLGKVNVLIGCGKVISIESDLNVQGIGDISIIDCKGKTVTPGLIDQHVHLIGGGGEGGFSSRTPQV
TFSKLIQAGTTSVVGVLGTDGISRSPRDLYAKAAALTEEGITAYMHTGSYEVPTKTITTSIRDDISFLPSVLGVKIALAD
HRCSFPTLQELSRIVSDIRIASLLAKKRGVLHMHMGALSDPFASVFELLNMGIPISHFSPTHVARTESLFSEAMQFARRG
GYIDITSGGSQFIAPELAIKQAIESGIDPKHITVSSDGNGSLPKFDIDGNMVALTAANVDGNLLLLPKLIESGISPEIAI
AMLTSNVADSLGINKGRLQLGQEADLCIFNDDFTLYGVVAKGKTVLLDNELLVTGNFE
>Mature_378_residues
MFILLKNANVYAPQHLGKVNVLIGCGKVISIESDLNVQGIGDISIIDCKGKTVTPGLIDQHVHLIGGGGEGGFSSRTPQV
TFSKLIQAGTTSVVGVLGTDGISRSPRDLYAKAAALTEEGITAYMHTGSYEVPTKTITTSIRDDISFLPSVLGVKIALAD
HRCSFPTLQELSRIVSDIRIASLLAKKRGVLHMHMGALSDPFASVFELLNMGIPISHFSPTHVARTESLFSEAMQFARRG
GYIDITSGGSQFIAPELAIKQAIESGIDPKHITVSSDGNGSLPKFDIDGNMVALTAANVDGNLLLLPKLIESGISPEIAI
AMLTSNVADSLGINKGRLQLGQEADLCIFNDDFTLYGVVAKGKTVLLDNELLVTGNFE

Specific function: Catalyzes the hydrolytic cleavage of a subset of L- isoaspartyl (L-beta-aspartyl) dipeptides. Used to degrade proteins damaged by L-isoaspartyl residues formation. The best substrate for the enzyme reported thus far is iso-Asp-Leu [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M38 family [H]

Homologues:

Organism=Escherichia coli, GI1790784, Length=380, Percent_Identity=42.6315789473684, Blast_Score=303, Evalue=2e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR011059
- InterPro:   IPR010229 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: 3.4.19.- [C]

Molecular weight: Translated: 40088; Mature: 40088

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFILLKNANVYAPQHLGKVNVLIGCGKVISIESDLNVQGIGDISIIDCKGKTVTPGLIDQ
CEEEEECCCEECCHHCCEEEEEEECCEEEEEECCCCEEECCCEEEEECCCCEECCCHHCC
HVHLIGGGGEGGFSSRTPQVTFSKLIQAGTTSVVGVLGTDGISRSPRDLYAKAAALTEEG
EEEEEECCCCCCCCCCCCCHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCC
ITAYMHTGSYEVPTKTITTSIRDDISFLPSVLGVKIALADHRCSFPTLQELSRIVSDIRI
CEEEEECCCEECCCHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHHHHHH
ASLLAKKRGVLHMHMGALSDPFASVFELLNMGIPISHFSPTHVARTESLFSEAMQFARRG
HHHHHHHCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
GYIDITSGGSQFIAPELAIKQAIESGIDPKHITVSSDGNGSLPKFDIDGNMVALTAANVD
CEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEECCCCEEEEEEECCC
GNLLLLPKLIESGISPEIAIAMLTSNVADSLGINKGRLQLGQEADLCIFNDDFTLYGVVA
CCEEEEHHHHHCCCCCCEEEEEEHHHHHHHHCCCCCCEEECCCCCEEEECCCCEEEEEEE
KGKTVLLDNELLVTGNFE
CCCEEEEECEEEEEECCC
>Mature Secondary Structure
MFILLKNANVYAPQHLGKVNVLIGCGKVISIESDLNVQGIGDISIIDCKGKTVTPGLIDQ
CEEEEECCCEECCHHCCEEEEEEECCEEEEEECCCCEEECCCEEEEECCCCEECCCHHCC
HVHLIGGGGEGGFSSRTPQVTFSKLIQAGTTSVVGVLGTDGISRSPRDLYAKAAALTEEG
EEEEEECCCCCCCCCCCCCHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCC
ITAYMHTGSYEVPTKTITTSIRDDISFLPSVLGVKIALADHRCSFPTLQELSRIVSDIRI
CEEEEECCCEECCCHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHHHHHH
ASLLAKKRGVLHMHMGALSDPFASVFELLNMGIPISHFSPTHVARTESLFSEAMQFARRG
HHHHHHHCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
GYIDITSGGSQFIAPELAIKQAIESGIDPKHITVSSDGNGSLPKFDIDGNMVALTAANVD
CEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEECCCCEEEEEEECCC
GNLLLLPKLIESGISPEIAIAMLTSNVADSLGINKGRLQLGQEADLCIFNDDFTLYGVVA
CCEEEEHHHHHCCCCCCEEEEEEHHHHHHHHCCCCCCEEECCCCCEEEECCCCEEEEEEE
KGKTVLLDNELLVTGNFE
CCCEEEEECEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7876157; 7610040; 9278503 [H]