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The map label for this gene is yfkN [H]

Identifier: 59712118

GI number: 59712118

Start: 1684618

End: 1686522

Strand: Reverse

Name: yfkN [H]

Synonym: VF_1511

Alternate gene names: 59712118

Gene position: 1686522-1684618 (Counterclockwise)

Preceding gene: 59712119

Following gene: 59712116

Centisome position: 58.21

GC content: 36.96

Gene sequence:

>1905_bases
ATGAAAAAACATATATTAACTTCAGCCATTTTACTTTCATTAAGTGGTTATGCAGCAGCGGCAGATGGCGATATTGTTGA
TGTTACGATCCTTGGTACATCTGATATTCATGGTCATTTTATGCCCTGGGATTATGCGAGCGACAAGCTCAATACGAAAG
GCAGTTTGAGTCAAATTGCTACTAAAGTGAAAACGATTCGTGATGAACAAGAAAATGTAATATTGGTAGATGCCGGTGAT
ACGATTCAAGGCAATTTTGTTGAAACTTTTAAGGATGAGCCTACAGATCCTATGATGCTTGGTTTCAACGAAATGAAATA
TGATATTTGGGTGTTAGGTAATCATGAATTTGATTTTGGCTTGAATGTACTTAATCGTTCATTGACTCAGTTTAAAGGGG
CATCATTAGGTGGGAATATAAAGCGAAAAGATGGCAATCCATTCTTACCTGCTTATAAAATTATTGAGAAAAATGGAATT
AAAATTGGTGTGATTGGTATGGATACACCAATGACACAAGTATTTGCTGAAGGTACGAATCGTCTTGAAGGTATGACTTT
TACCAATCCGTCCCTTGAAGTGAAAAAAGTCATTAAAGAAATAGATGATAAAGTAGATGCGATTGTGCTAGTTGTTCATA
TGGGGCTTGATAATGAGAATAACATTGAAGCCACAGGGGTTACGGATATCGCTAATGATAACCCTGAAATTGATGCTATT
GTTGCCGGTCACATGCATACCCGTATTGATAAAGCGACAGTAAATGGTGTGATTATAACGGAACCAGATAAGTATGGCCG
TGCGCTTTCTCGTATCGATTTACAATTTGAAGAGAAGAATGGACAGTTCACTCTAGTTAATAAAGACAGTTTTACGTACA
AGATAAAAGGGGTTGAATCAGACGAGAAAATGGAACAACTGTATGCTCCATATCATAAACGTCTGCGTGAAAATGCAAAC
CGTGTCGTCGCAGAATTACTCGGTGTTGATTTAGTACCTGAAAATGAAATTAAAGGTATCCCACAAGTTCATATTCAGGA
TACAGGGATCAGTGCTTTGTTCCAAGAAGCCAGTATGCATTATGCTCCCTTAGCAAACGTGATTGCTTTGCAAATTGATA
ACGATATGGCAGAGCTTAATGTAGGTAAAATCAAAGCCAAAGATATTGCATATAATTATCAGTATGCAGGTGGTGAAATT
ACTGTTTATGAAATGACAGGTAAAGAGCTGAAAACATATATGGAGTGGTCAGCAGGTTATTTCAATAGCGTAAAAGAAGG
GGATGTGACATACAGTTTTAACCTTGATCGTCGCTCATCAAAATATTCAACCAATGATTTCTTTGCTGGTGTGACTTATG
TAATAGATCTAACAAAGCCGACTGGTGAACGTATTACCGATCTTAAATTTGCCGATGGTAGAGATATTACGAATAAAACG
CCAATTCGTATCGGAATGAACAGCTATCGAATGGGGCATTTAACCAAAGATGGTGGTGTGCTTGAAGGTAGACATTTCCC
AGTATTGTTTGATACCGAAGAAGAGTTTGGAGAAGAAGATGGAACAATTCGGAACTTAACTATTCACTACCTAGATGAGG
TAAAAAAAGGTAAATACGAAGGTAAGCCAATGAGTCGCTGGACATTGATTGGTCTTGAAGGTTATGAGAAAGAACGAGAA
ATAGTAAAGGCGTTAATTAACAGTGGCAAAATAGACGTACCAAAAAGCAAAGATGGCCGATATACCAATGTTGAATCTAT
TAATGTAAAAGATAAGATATTTTCAACGGAAGCTGAATTAACGACTTATTTAAGTAAACTTGAACAATCTTTAGATAAGG
CAACAACTGAGGTTGAAAAAGCACGAATTCATCGTGATATGGTAATTGCAAAGGCGCTGAATTAA

Upstream 100 bases:

>100_bases
TTGCCTTTTCTTGTTACATTTCAAATGTGATCGATATTTCTTTATGAGGGGAGATAACGATTTATGATCGAGTTTCCCTA
CATAAAAAATAAAGATAATA

Downstream 100 bases:

>100_bases
AAACAAAAAAGGTGGCTTAGATTGGCCACCTTTAATTTTTCAAGCTATGTATTTCTTCTAAAAATTGCAGTACAGCAGGA
CGACTATTAGCTTTCGAATG

Product: 2',3'-cyclic-nucleotide 2'-phosphodiesterase

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 634; Mature: 634

Protein sequence:

>634_residues
MKKHILTSAILLSLSGYAAAADGDIVDVTILGTSDIHGHFMPWDYASDKLNTKGSLSQIATKVKTIRDEQENVILVDAGD
TIQGNFVETFKDEPTDPMMLGFNEMKYDIWVLGNHEFDFGLNVLNRSLTQFKGASLGGNIKRKDGNPFLPAYKIIEKNGI
KIGVIGMDTPMTQVFAEGTNRLEGMTFTNPSLEVKKVIKEIDDKVDAIVLVVHMGLDNENNIEATGVTDIANDNPEIDAI
VAGHMHTRIDKATVNGVIITEPDKYGRALSRIDLQFEEKNGQFTLVNKDSFTYKIKGVESDEKMEQLYAPYHKRLRENAN
RVVAELLGVDLVPENEIKGIPQVHIQDTGISALFQEASMHYAPLANVIALQIDNDMAELNVGKIKAKDIAYNYQYAGGEI
TVYEMTGKELKTYMEWSAGYFNSVKEGDVTYSFNLDRRSSKYSTNDFFAGVTYVIDLTKPTGERITDLKFADGRDITNKT
PIRIGMNSYRMGHLTKDGGVLEGRHFPVLFDTEEEFGEEDGTIRNLTIHYLDEVKKGKYEGKPMSRWTLIGLEGYEKERE
IVKALINSGKIDVPKSKDGRYTNVESINVKDKIFSTEAELTTYLSKLEQSLDKATTEVEKARIHRDMVIAKALN

Sequences:

>Translated_634_residues
MKKHILTSAILLSLSGYAAAADGDIVDVTILGTSDIHGHFMPWDYASDKLNTKGSLSQIATKVKTIRDEQENVILVDAGD
TIQGNFVETFKDEPTDPMMLGFNEMKYDIWVLGNHEFDFGLNVLNRSLTQFKGASLGGNIKRKDGNPFLPAYKIIEKNGI
KIGVIGMDTPMTQVFAEGTNRLEGMTFTNPSLEVKKVIKEIDDKVDAIVLVVHMGLDNENNIEATGVTDIANDNPEIDAI
VAGHMHTRIDKATVNGVIITEPDKYGRALSRIDLQFEEKNGQFTLVNKDSFTYKIKGVESDEKMEQLYAPYHKRLRENAN
RVVAELLGVDLVPENEIKGIPQVHIQDTGISALFQEASMHYAPLANVIALQIDNDMAELNVGKIKAKDIAYNYQYAGGEI
TVYEMTGKELKTYMEWSAGYFNSVKEGDVTYSFNLDRRSSKYSTNDFFAGVTYVIDLTKPTGERITDLKFADGRDITNKT
PIRIGMNSYRMGHLTKDGGVLEGRHFPVLFDTEEEFGEEDGTIRNLTIHYLDEVKKGKYEGKPMSRWTLIGLEGYEKERE
IVKALINSGKIDVPKSKDGRYTNVESINVKDKIFSTEAELTTYLSKLEQSLDKATTEVEKARIHRDMVIAKALN
>Mature_634_residues
MKKHILTSAILLSLSGYAAAADGDIVDVTILGTSDIHGHFMPWDYASDKLNTKGSLSQIATKVKTIRDEQENVILVDAGD
TIQGNFVETFKDEPTDPMMLGFNEMKYDIWVLGNHEFDFGLNVLNRSLTQFKGASLGGNIKRKDGNPFLPAYKIIEKNGI
KIGVIGMDTPMTQVFAEGTNRLEGMTFTNPSLEVKKVIKEIDDKVDAIVLVVHMGLDNENNIEATGVTDIANDNPEIDAI
VAGHMHTRIDKATVNGVIITEPDKYGRALSRIDLQFEEKNGQFTLVNKDSFTYKIKGVESDEKMEQLYAPYHKRLRENAN
RVVAELLGVDLVPENEIKGIPQVHIQDTGISALFQEASMHYAPLANVIALQIDNDMAELNVGKIKAKDIAYNYQYAGGEI
TVYEMTGKELKTYMEWSAGYFNSVKEGDVTYSFNLDRRSSKYSTNDFFAGVTYVIDLTKPTGERITDLKFADGRDITNKT
PIRIGMNSYRMGHLTKDGGVLEGRHFPVLFDTEEEFGEEDGTIRNLTIHYLDEVKKGKYEGKPMSRWTLIGLEGYEKERE
IVKALINSGKIDVPKSKDGRYTNVESINVKDKIFSTEAELTTYLSKLEQSLDKATTEVEKARIHRDMVIAKALN

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=300, Percent_Identity=25.6666666666667, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1790658, Length=614, Percent_Identity=25.0814332247557, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1786687, Length=457, Percent_Identity=27.5711159737418, Blast_Score=95, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24641187, Length=490, Percent_Identity=24.2857142857143, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI19922444, Length=248, Percent_Identity=28.6290322580645, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 70942; Mature: 70942

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKHILTSAILLSLSGYAAAADGDIVDVTILGTSDIHGHFMPWDYASDKLNTKGSLSQIA
CCCHHHHHHHHHHHCCCEEECCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHH
TKVKTIRDEQENVILVDAGDTIQGNFVETFKDEPTDPMMLGFNEMKYDIWVLGNHEFDFG
HHHHHHHCCCCCEEEEECCCCCCCCCHHHCCCCCCCCEEECCCCEEEEEEEEECCCCCCC
LNVLNRSLTQFKGASLGGNIKRKDGNPFLPAYKIIEKNGIKIGVIGMDTPMTQVFAEGTN
HHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHEEECCCCEEEEEECCCCHHHHHHCCCC
RLEGMTFTNPSLEVKKVIKEIDDKVDAIVLVVHMGLDNENNIEATGVTDIANDNPEIDAI
CCCCEEECCCCHHHHHHHHHHHHHHCEEEEEEEECCCCCCCEEECCCEECCCCCCCEEEE
VAGHMHTRIDKATVNGVIITEPDKYGRALSRIDLQFEEKNGQFTLVNKDSFTYKIKGVES
EECCHHHHCCCEEECEEEEECCHHHHHHHHHEEEEEEECCCEEEEEECCCEEEEEECCCC
DEKMEQLYAPYHKRLRENANRVVAELLGVDLVPENEIKGIPQVHIQDTGISALFQEASMH
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCEEEEECCCHHHHHHHHCCC
YAPLANVIALQIDNDMAELNVGKIKAKDIAYNYQYAGGEITVYEMTGKELKTYMEWSAGY
CCCCCEEEEEEECCCHHHCCCCEEEEEEEEEEEEECCCEEEEEEECCHHHHHHHHHCCCC
FNSVKEGDVTYSFNLDRRSSKYSTNDFFAGVTYVIDLTKPTGERITDLKFADGRDITNKT
CCCCCCCCEEEEEECCCCCCCCCCCCCEEEEEEEEECCCCCCCCEEEEEECCCCCCCCCC
PIRIGMNSYRMGHLTKDGGVLEGRHFPVLFDTEEEFGEEDGTIRNLTIHYLDEVKKGKYE
CEEECCCCEEECEEECCCCEECCCCCEEEECCHHHHCCCCCCEEEEEEHHHHHHHCCCCC
GKPMSRWTLIGLEGYEKEREIVKALINSGKIDVPKSKDGRYTNVESINVKDKIFSTEAEL
CCCCCEEEEEECCCCHHHHHHHHHHHCCCCEECCCCCCCCEECEEEECCHHHHCCCHHHH
TTYLSKLEQSLDKATTEVEKARIHRDMVIAKALN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKKHILTSAILLSLSGYAAAADGDIVDVTILGTSDIHGHFMPWDYASDKLNTKGSLSQIA
CCCHHHHHHHHHHHCCCEEECCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHH
TKVKTIRDEQENVILVDAGDTIQGNFVETFKDEPTDPMMLGFNEMKYDIWVLGNHEFDFG
HHHHHHHCCCCCEEEEECCCCCCCCCHHHCCCCCCCCEEECCCCEEEEEEEEECCCCCCC
LNVLNRSLTQFKGASLGGNIKRKDGNPFLPAYKIIEKNGIKIGVIGMDTPMTQVFAEGTN
HHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHEEECCCCEEEEEECCCCHHHHHHCCCC
RLEGMTFTNPSLEVKKVIKEIDDKVDAIVLVVHMGLDNENNIEATGVTDIANDNPEIDAI
CCCCEEECCCCHHHHHHHHHHHHHHCEEEEEEEECCCCCCCEEECCCEECCCCCCCEEEE
VAGHMHTRIDKATVNGVIITEPDKYGRALSRIDLQFEEKNGQFTLVNKDSFTYKIKGVES
EECCHHHHCCCEEECEEEEECCHHHHHHHHHEEEEEEECCCEEEEEECCCEEEEEECCCC
DEKMEQLYAPYHKRLRENANRVVAELLGVDLVPENEIKGIPQVHIQDTGISALFQEASMH
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCEEEEECCCHHHHHHHHCCC
YAPLANVIALQIDNDMAELNVGKIKAKDIAYNYQYAGGEITVYEMTGKELKTYMEWSAGY
CCCCCEEEEEEECCCHHHCCCCEEEEEEEEEEEEECCCEEEEEEECCHHHHHHHHHCCCC
FNSVKEGDVTYSFNLDRRSSKYSTNDFFAGVTYVIDLTKPTGERITDLKFADGRDITNKT
CCCCCCCCEEEEEECCCCCCCCCCCCCEEEEEEEEECCCCCCCCEEEEEECCCCCCCCCC
PIRIGMNSYRMGHLTKDGGVLEGRHFPVLFDTEEEFGEEDGTIRNLTIHYLDEVKKGKYE
CEEECCCCEEECEEECCCCEECCCCCEEEECCHHHHCCCCCCEEEEEEHHHHHHHCCCCC
GKPMSRWTLIGLEGYEKEREIVKALINSGKIDVPKSKDGRYTNVESINVKDKIFSTEAEL
CCCCCEEEEEECCCCHHHHHHHHHHHCCCCEECCCCCCCCEECEEEECCHHHHCCCHHHH
TTYLSKLEQSLDKATTEVEKARIHRDMVIAKALN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]