Click here to switch to the map view.

The map label for this gene is purT

Identifier: 59712094

GI number: 59712094

Start: 1645646

End: 1646821

Strand: Direct

Name: purT

Synonym: VF_1487

Alternate gene names: 59712094

Gene position: 1645646-1646821 (Clockwise)

Preceding gene: 59712092

Following gene: 59712095

Centisome position: 56.79

GC content: 42.6

Gene sequence:

>1176_bases
ATGTTTGGTACAGCTCTGTCTTCTTCTGCAACAAAAGTATTATTACTTGGTTCTGGTGAACTAGGTAAAGAAGTGGCTAT
TGAATGTCAGCGTCTTGGTTTTGAAGTAATTGCGGTTGATCGCTACCTTAATGCTCCGGCAATGCAAGTGGCTCACCGCA
GTTACGCGATTGATATGCTTGATGCCAACGCCTTAGAAGAAGTCATTAATAAAGAACAACCTGATTTTGTGGTCCCTGAA
ATTGAAGCGATTGCAACGGATAAGTTAGTTGAACTAGAGAAACAAGGCCTTAATGTTGTTCCAACGGCTAACGCGACAAA
ATTAACCATGAACCGTGAAGGTATTCGCCGTTTAGCCGCTGAAGAATTACAACTGCCGACGTCTCCTTATCGCTTTGTTG
ATAGCTATGAAGAACTTGTTGAAGCGGTGAAATTTGTCGGTATGCCTTGCGTTATTAAACCGGTAATGAGCTCTTCTGGT
AAAGGTCAAAGCGTTATCAAAACAGAAGCAGACATCCGTACTTCTTGGGATTATGCGCAAGATGGTGGCCGCTCTGGTGC
TGGACGAGTCATTGTTGAAGGGTTTGTGGATTTTGATTATGAGATTTCATTATTAACCGTTCGTGCTGTTGATGGTGTTC
ATTTCTGCGCGCCAATTGGCCATCGTCAAGAAGATGGTGACTACCGTGAATCATGGCAGCCACAAGTGATGACTGAGGCC
GCATTAAAACAAGCACAAAATGCGGCAGAGAAAGTGGTAAACGCCCTTGGCGGTTATGGCTTATTTGGTGTAGAGCTTTT
CATTAAAGGCGATAACATTTACTTTAATGAAGTCTCTCCTCGCCCACACGACACAGGTCTTGTGACACTTATTTCTCAAG
AAATGTCTGAATTTGCCCTTCATGTTCGTGCATTTACTGGAATGCCAATTAATCAAGTTACACAATACGGTCCTTCGGCA
TCGTCAGTAATTTTAGGTCAAGGCACTTCGACCAATATTCGTTTTGATGGCTTAACCGAAGCGCTTTCTCAGCCTCAAAC
TCAAATCAAATTATTCGGTAAACCAGAGATTGATGGACGTCGACGTTTAGGTGTTGCGATTACACGTCGTGATAATTTAG
AAACCGCTATTGAGGATGCAGTAACGGCTTCAAATAAAGTTCAAGTAATCTATTAA

Upstream 100 bases:

>100_bases
TAATTCCATTCTATTTATAAGGAATAAATAGCAAACGTTTGCGTATTTAATTAATTTTAGTATGATAGCGCCATCTTTCT
TTATTACATGAGAATTTCAT

Downstream 100 bases:

>100_bases
GTTACAAATATGTATAGCAGAATGGTTTCAAATACCGTTCTGTTGTACTTTTTTAACCTTCTTATTCTTTTTTTACTGCT
AAATCCATTTAATTTGCTTT

Product: phosphoribosylglycinamide formyltransferase 2

Products: tetrahydrofolate; N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MFGTALSSSATKVLLLGSGELGKEVAIECQRLGFEVIAVDRYLNAPAMQVAHRSYAIDMLDANALEEVINKEQPDFVVPE
IEAIATDKLVELEKQGLNVVPTANATKLTMNREGIRRLAAEELQLPTSPYRFVDSYEELVEAVKFVGMPCVIKPVMSSSG
KGQSVIKTEADIRTSWDYAQDGGRSGAGRVIVEGFVDFDYEISLLTVRAVDGVHFCAPIGHRQEDGDYRESWQPQVMTEA
ALKQAQNAAEKVVNALGGYGLFGVELFIKGDNIYFNEVSPRPHDTGLVTLISQEMSEFALHVRAFTGMPINQVTQYGPSA
SSVILGQGTSTNIRFDGLTEALSQPQTQIKLFGKPEIDGRRRLGVAITRRDNLETAIEDAVTASNKVQVIY

Sequences:

>Translated_391_residues
MFGTALSSSATKVLLLGSGELGKEVAIECQRLGFEVIAVDRYLNAPAMQVAHRSYAIDMLDANALEEVINKEQPDFVVPE
IEAIATDKLVELEKQGLNVVPTANATKLTMNREGIRRLAAEELQLPTSPYRFVDSYEELVEAVKFVGMPCVIKPVMSSSG
KGQSVIKTEADIRTSWDYAQDGGRSGAGRVIVEGFVDFDYEISLLTVRAVDGVHFCAPIGHRQEDGDYRESWQPQVMTEA
ALKQAQNAAEKVVNALGGYGLFGVELFIKGDNIYFNEVSPRPHDTGLVTLISQEMSEFALHVRAFTGMPINQVTQYGPSA
SSVILGQGTSTNIRFDGLTEALSQPQTQIKLFGKPEIDGRRRLGVAITRRDNLETAIEDAVTASNKVQVIY
>Mature_391_residues
MFGTALSSSATKVLLLGSGELGKEVAIECQRLGFEVIAVDRYLNAPAMQVAHRSYAIDMLDANALEEVINKEQPDFVVPE
IEAIATDKLVELEKQGLNVVPTANATKLTMNREGIRRLAAEELQLPTSPYRFVDSYEELVEAVKFVGMPCVIKPVMSSSG
KGQSVIKTEADIRTSWDYAQDGGRSGAGRVIVEGFVDFDYEISLLTVRAVDGVHFCAPIGHRQEDGDYRESWQPQVMTEA
ALKQAQNAAEKVVNALGGYGLFGVELFIKGDNIYFNEVSPRPHDTGLVTLISQEMSEFALHVRAFTGMPINQVTQYGPSA
SSVILGQGTSTNIRFDGLTEALSQPQTQIKLFGKPEIDGRRRLGVAITRRDNLETAIEDAVTASNKVQVIY

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1788155, Length=389, Percent_Identity=67.6092544987146, Blast_Score=548, Evalue=1e-157,
Organism=Escherichia coli, GI1786733, Length=365, Percent_Identity=23.5616438356164, Blast_Score=81, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324702, Length=365, Percent_Identity=23.8356164383562, Blast_Score=100, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURT_VIBF1 (Q5E4R4)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_204870.1
- HSSP:   P33221
- ProteinModelPortal:   Q5E4R4
- SMR:   Q5E4R4
- STRING:   Q5E4R4
- GeneID:   3277634
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_1487
- NMPDR:   fig|312309.3.peg.1487
- eggNOG:   COG0027
- HOGENOM:   HBG516369
- OMA:   PDYIVPE
- PhylomeDB:   Q5E4R4
- ProtClustDB:   PRK09288
- BioCyc:   VFIS312309:VF1487-MONOMER
- HAMAP:   MF_01643
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862
- InterPro:   IPR011054
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- PANTHER:   PTHR23047:SF2
- TIGRFAMs:   TIGR01142

Pfam domain/function: PF02222 ATP-grasp; SSF52440 PreATP-grasp-like; SSF51246 Rudmnt_hyb_motif

EC number: 2.1.2.2

Molecular weight: Translated: 42751; Mature: 42751

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS50975 ATP_GRASP; PS00761 SPASE_I_3

Important sites: BINDING 80-80 BINDING 112-112 BINDING 153-153 BINDING 201-201 BINDING 265-265 BINDING 277-277 BINDING 284-284 BINDING 354-354

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGTALSSSATKVLLLGSGELGKEVAIECQRLGFEVIAVDRYLNAPAMQVAHRSYAIDML
CCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCEEEEEEEHHCCCHHHHHHHHHHEEEEC
DANALEEVINKEQPDFVVPEIEAIATDKLVELEKQGLNVVPTANATKLTMNREGIRRLAA
CHHHHHHHHCCCCCCEECCCHHHHHHHHHHHHHHCCCEEEECCCCEEEEECHHHHHHHHH
EELQLPTSPYRFVDSYEELVEAVKFVGMPCVIKPVMSSSGKGQSVIKTEADIRTSWDYAQ
HHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEHHHHCCCCCCCEEEEECCCCCCCCCHHH
DGGRSGAGRVIVEGFVDFDYEISLLTVRAVDGVHFCAPIGHRQEDGDYRESWQPQVMTEA
CCCCCCCCEEEEEEEECCCEEEEEEEEEEECCEEEECCCCCCCCCCCHHHCCCCHHHHHH
ALKQAQNAAEKVVNALGGYGLFGVELFIKGDNIYFNEVSPRPHDTGLVTLISQEMSEFAL
HHHHHHHHHHHHHHHHCCCCEEEEEEEEECCEEEEECCCCCCCCCCHHHHHHHHHHHHHH
HVRAFTGMPINQVTQYGPSASSVILGQGTSTNIRFDGLTEALSQPQTQIKLFGKPEIDGR
HHHHHCCCCHHHHHHCCCCCCEEEEECCCCCCEEECCHHHHHCCCCEEEEEECCCCCCCC
RRLGVAITRRDNLETAIEDAVTASNKVQVIY
CEEEEEEEECCCHHHHHHHHHCCCCCEEEEC
>Mature Secondary Structure
MFGTALSSSATKVLLLGSGELGKEVAIECQRLGFEVIAVDRYLNAPAMQVAHRSYAIDML
CCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCEEEEEEEHHCCCHHHHHHHHHHEEEEC
DANALEEVINKEQPDFVVPEIEAIATDKLVELEKQGLNVVPTANATKLTMNREGIRRLAA
CHHHHHHHHCCCCCCEECCCHHHHHHHHHHHHHHCCCEEEECCCCEEEEECHHHHHHHHH
EELQLPTSPYRFVDSYEELVEAVKFVGMPCVIKPVMSSSGKGQSVIKTEADIRTSWDYAQ
HHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEHHHHCCCCCCCEEEEECCCCCCCCCHHH
DGGRSGAGRVIVEGFVDFDYEISLLTVRAVDGVHFCAPIGHRQEDGDYRESWQPQVMTEA
CCCCCCCCEEEEEEEECCCEEEEEEEEEEECCEEEECCCCCCCCCCCHHHCCCCHHHHHH
ALKQAQNAAEKVVNALGGYGLFGVELFIKGDNIYFNEVSPRPHDTGLVTLISQEMSEFAL
HHHHHHHHHHHHHHHHCCCCEEEEEEEEECCEEEEECCCCCCCCCCHHHHHHHHHHHHHH
HVRAFTGMPINQVTQYGPSASSVILGQGTSTNIRFDGLTEALSQPQTQIKLFGKPEIDGR
HHHHHCCCCHHHHHHCCCCCCEEEEECCCCCCEEECCHHHHHCCCCEEEEEECCCCCCCC
RRLGVAITRRDNLETAIEDAVTASNKVQVIY
CEEEEEEEECCCHHHHHHHHHCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 10-formyltetrahydrofolate; N1-(5-phospho-D-ribosyl)glycinamide

Specific reaction: 10-formyltetrahydrofolate + N1-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA