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The map label for this gene is nagK

Identifier: 59712015

GI number: 59712015

Start: 1557444

End: 1558355

Strand: Reverse

Name: nagK

Synonym: VF_1408

Alternate gene names: 59712015

Gene position: 1558355-1557444 (Counterclockwise)

Preceding gene: 172087706

Following gene: 59712008

Centisome position: 53.78

GC content: 39.58

Gene sequence:

>912_bases
ATGTACTACGGTTTTGATGTGGGTGGAACCAAGATTGAGTTTGGTGCGTTTAATGAAAAACTTGAGCGTGTCGCAACTGA
GCGAATTCCAACACAAACTGAAAATTACTCGTTATTAGTTGATGATATTGCATCATTGATTGCAAAGTATGATGCAGAGT
TTGGCGTTGAAGGTAAAGTCGGTTTAGGTATTCCGGGAATGGAAGATGCTGAAACTGGTGCTTTATTAACAAGTAATGTT
CCGGCTGCAAAAGGGCAATTTTTACGTAAAGATTTAGAAGCAAAAATTGGTCGTTCAGTAAAAATTGATAACGATGCTAA
CTGTTTTGCATTATCAGAAGCATGGGATGAAGAGTTAAAAGATTCACCATCAGTGATGGGATTAATTCTAGGGACTGGTT
TTGGTGGTGGTTTAATTTTTGATGGTAAGGCCTTCTCGGGTTACAGCCATGTTGCTGGTGAATTAGGTCATTCACGTTTA
CCTATTGATGCATGGTTTCATTTAGGTGAAAACGCACCATTACTTGAATGTGGCTGTGGTAACAAAGGCTGTATTGATAA
CTACCTTTCTGGACGTGGCTTTGAGCTGCTTTACGCGCATTACTATGGTGAGCAAAAGAAAGCGATTGATATTATTAAAG
CTCATGCTGAAGGTGATGCGAATGCAGTAGAACATGTTGATCGTTTCATGGAGCTCTTAGCAATCTGTTTCGCTAATTTA
TTTACTTGTTTTGACCCACACGTTGTCGCTCTTGGTGGTGGTTTATCTAACTTTGAGTTGATTTACGAAGAGCTACCAAA
GCGTTTACCAAAACATTTATTATCAGTAGGTCGCGTTCCTCGTATTATTAAAGCGAAGCACGGTGATTCTGGTGGTGTGC
GTGGTGCAGCATTCTTGAATATTAAAGACTAA

Upstream 100 bases:

>100_bases
TAAAAACCGATGAATATCGGATAGGTCACAGTCTGTATAGCTAAAAATTAGGTAAGATAACACCATAAAAATTTAGAATA
GATTAGGACAAGGATACCTT

Downstream 100 bases:

>100_bases
GTTCTACAAAAATAAGATAGAAATTTAGATACAAAAAGAGCCTCATGTTGTTTGTTATTAGTAACAGATAAACAGAGGCT
CTTTTTTGTAATGCGCTTAG

Product: N-acetyl-D-glucosamine kinase

Products: NA

Alternate protein names: GlcNAc kinase

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MYYGFDVGGTKIEFGAFNEKLERVATERIPTQTENYSLLVDDIASLIAKYDAEFGVEGKVGLGIPGMEDAETGALLTSNV
PAAKGQFLRKDLEAKIGRSVKIDNDANCFALSEAWDEELKDSPSVMGLILGTGFGGGLIFDGKAFSGYSHVAGELGHSRL
PIDAWFHLGENAPLLECGCGNKGCIDNYLSGRGFELLYAHYYGEQKKAIDIIKAHAEGDANAVEHVDRFMELLAICFANL
FTCFDPHVVALGGGLSNFELIYEELPKRLPKHLLSVGRVPRIIKAKHGDSGGVRGAAFLNIKD

Sequences:

>Translated_303_residues
MYYGFDVGGTKIEFGAFNEKLERVATERIPTQTENYSLLVDDIASLIAKYDAEFGVEGKVGLGIPGMEDAETGALLTSNV
PAAKGQFLRKDLEAKIGRSVKIDNDANCFALSEAWDEELKDSPSVMGLILGTGFGGGLIFDGKAFSGYSHVAGELGHSRL
PIDAWFHLGENAPLLECGCGNKGCIDNYLSGRGFELLYAHYYGEQKKAIDIIKAHAEGDANAVEHVDRFMELLAICFANL
FTCFDPHVVALGGGLSNFELIYEELPKRLPKHLLSVGRVPRIIKAKHGDSGGVRGAAFLNIKD
>Mature_303_residues
MYYGFDVGGTKIEFGAFNEKLERVATERIPTQTENYSLLVDDIASLIAKYDAEFGVEGKVGLGIPGMEDAETGALLTSNV
PAAKGQFLRKDLEAKIGRSVKIDNDANCFALSEAWDEELKDSPSVMGLILGTGFGGGLIFDGKAFSGYSHVAGELGHSRL
PIDAWFHLGENAPLLECGCGNKGCIDNYLSGRGFELLYAHYYGEQKKAIDIIKAHAEGDANAVEHVDRFMELLAICFANL
FTCFDPHVVALGGGLSNFELIYEELPKRLPKHLLSVGRVPRIIKAKHGDSGGVRGAAFLNIKD

Specific function: Catalyzes the phosphorylation of N-acetyl-D-glucosamine (GlcNAc) derived from cell-wall degradation, yielding GlcNAc-6-P

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family. NagK subfamily

Homologues:

Organism=Escherichia coli, GI1787363, Length=303, Percent_Identity=54.7854785478548, Blast_Score=358, Evalue=1e-100,
Organism=Escherichia coli, GI87081733, Length=304, Percent_Identity=37.171052631579, Blast_Score=194, Evalue=7e-51,
Organism=Escherichia coli, GI1790522, Length=308, Percent_Identity=27.2727272727273, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NAGK_VIBF1 (Q5E4Z3)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_204791.1
- HSSP:   Q8ZPZ9
- ProteinModelPortal:   Q5E4Z3
- SMR:   Q5E4Z3
- STRING:   Q5E4Z3
- GeneID:   3278741
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_1408
- NMPDR:   fig|312309.3.peg.1408
- eggNOG:   COG1940
- HOGENOM:   HBG730260
- OMA:   GMEDADD
- PhylomeDB:   Q5E4Z3
- ProtClustDB:   PRK13310
- BioCyc:   VFIS312309:VF1408-MONOMER
- HAMAP:   MF_01271
- InterPro:   IPR000600

Pfam domain/function: PF00480 ROK

EC number: =2.7.1.59

Molecular weight: Translated: 32794; Mature: 32794

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS01125 ROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYYGFDVGGTKIEFGAFNEKLERVATERIPTQTENYSLLVDDIASLIAKYDAEFGVEGKV
CEEEECCCCCEEEECHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHCCCCCCCCCC
GLGIPGMEDAETGALLTSNVPAAKGQFLRKDLEAKIGRSVKIDNDANCFALSEAWDEELK
CCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCEEEECCCCCEEEEHHHHHHHHH
DSPSVMGLILGTGFGGGLIFDGKAFSGYSHVAGELGHSRLPIDAWFHLGENAPLLECGCG
CCCCEEEEEEECCCCCCEEECCCCCCCHHHHHHHCCCCCCCHHHHHCCCCCCCEEEECCC
NKGCIDNYLSGRGFELLYAHYYGEQKKAIDIIKAHAEGDANAVEHVDRFMELLAICFANL
CCCCHHHHHCCCCCEEEEEEHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
FTCFDPHVVALGGGLSNFELIYEELPKRLPKHLLSVGRVPRIIKAKHGDSGGVRGAAFLN
HHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCEEEEEE
IKD
ECC
>Mature Secondary Structure
MYYGFDVGGTKIEFGAFNEKLERVATERIPTQTENYSLLVDDIASLIAKYDAEFGVEGKV
CEEEECCCCCEEEECHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHCCCCCCCCCC
GLGIPGMEDAETGALLTSNVPAAKGQFLRKDLEAKIGRSVKIDNDANCFALSEAWDEELK
CCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCEEEECCCCCEEEEHHHHHHHHH
DSPSVMGLILGTGFGGGLIFDGKAFSGYSHVAGELGHSRLPIDAWFHLGENAPLLECGCG
CCCCEEEEEEECCCCCCEEECCCCCCCHHHHHHHCCCCCCCHHHHHCCCCCCCEEEECCC
NKGCIDNYLSGRGFELLYAHYYGEQKKAIDIIKAHAEGDANAVEHVDRFMELLAICFANL
CCCCHHHHHCCCCCEEEEEEHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
FTCFDPHVVALGGGLSNFELIYEELPKRLPKHLLSVGRVPRIIKAKHGDSGGVRGAAFLN
HHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCEEEEEE
IKD
ECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA