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The map label for this gene is ampR [H]

Identifier: 59711940

GI number: 59711940

Start: 1481053

End: 1482009

Strand: Direct

Name: ampR [H]

Synonym: VF_1333

Alternate gene names: 59711940

Gene position: 1481053-1482009 (Clockwise)

Preceding gene: 59711937

Following gene: 59711941

Centisome position: 51.11

GC content: 38.04

Gene sequence:

>957_bases
ATGACACCCTATCCAACATTACCTTACTCACATAACGGCTTTAAAGCTTTTGAATCTGTTGCAAGGTTGATGAGTTTTAC
CTCAGCGGCAAATGAGCTTAATGTGACTCAAAGTGCAGTCAGCCGCCAAGTAAAGCAACTTGAAGATGATCTCAACGCAT
CTCTAATCATACGCAAACACCGTTCGATTCAACTGACGACACAAGGTTTAGAACTGTATAAAACGTTACAGGATAACTTT
ACTTCCCTCGAATCAGTGATTTCATCTTGGCAAGACCCAAAGCCTAAACGTATTGTTATCAAAGCAGCATTAAGCTTTGC
TACCCGTGCTCTCTTACCTAAAATCAAAGAAATGAATGAGAGATATTCAGATTATGAAATCGTCATCATTCCTTCTTTAG
AAGAAGACGAAGCCCTCAATACCACTGATTATGATTTATTGATTTTCCATACAAGGTTAAGGAATCATTACGATAATAAA
TCTAATTTTTCTCTGTTACGTGAAGAATTTATGGCACCGGTTTGTGCAACCAGCATTACTACAAAAGACACGGTGTTAGA
TGATGTTCTTCAATTACCAAGGTTGCATCCTACAGTTGATCATCTCGATTGGATTACTTGGCTAAATAATGAAAAATATG
TTCCCCATCAAAAAGTGAGAAACACCAGTTTTCACTCATTAGATCTTGCATTAAGTGCGTGTTTGTCAGGTCAAGGCGCA
ACCGTAACTGATCTCTTATTGATCTTACCGGAATTAGAAAGAAACTTTTATTACTGCCCAAGGCACACCACACTTCAACA
CAGCGCATGGGTATATTATATCCATCAACACACGCATTCTTCAGCGATTAACGAGCTTATCGAATGGTTACAGCAAGAGG
CAAAACGTGAGATTGAATCATTAAAATGTTTAGCTAAAACGCATCATTGGGGAGGAATTATTAACCAGATTAATTAG

Upstream 100 bases:

>100_bases
GATAGATTAAATAATTTCACATCACGTTATCGTTTAAAAAAGTAGTATTCAATTAATTAATCCGTCAGACTGGCATTCCA
AAAACTCATGCGAGAATCTA

Downstream 100 bases:

>100_bases
GTAGATATTTATTGATAAAAAATAAATTTCAGAATACTCTGTAATGCTGCGTTGTTTTTACCGCACTAACTGATCATCAG
TTTTAACATCGAATTCGATA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MTPYPTLPYSHNGFKAFESVARLMSFTSAANELNVTQSAVSRQVKQLEDDLNASLIIRKHRSIQLTTQGLELYKTLQDNF
TSLESVISSWQDPKPKRIVIKAALSFATRALLPKIKEMNERYSDYEIVIIPSLEEDEALNTTDYDLLIFHTRLRNHYDNK
SNFSLLREEFMAPVCATSITTKDTVLDDVLQLPRLHPTVDHLDWITWLNNEKYVPHQKVRNTSFHSLDLALSACLSGQGA
TVTDLLLILPELERNFYYCPRHTTLQHSAWVYYIHQHTHSSAINELIEWLQQEAKREIESLKCLAKTHHWGGIINQIN

Sequences:

>Translated_318_residues
MTPYPTLPYSHNGFKAFESVARLMSFTSAANELNVTQSAVSRQVKQLEDDLNASLIIRKHRSIQLTTQGLELYKTLQDNF
TSLESVISSWQDPKPKRIVIKAALSFATRALLPKIKEMNERYSDYEIVIIPSLEEDEALNTTDYDLLIFHTRLRNHYDNK
SNFSLLREEFMAPVCATSITTKDTVLDDVLQLPRLHPTVDHLDWITWLNNEKYVPHQKVRNTSFHSLDLALSACLSGQGA
TVTDLLLILPELERNFYYCPRHTTLQHSAWVYYIHQHTHSSAINELIEWLQQEAKREIESLKCLAKTHHWGGIINQIN
>Mature_317_residues
TPYPTLPYSHNGFKAFESVARLMSFTSAANELNVTQSAVSRQVKQLEDDLNASLIIRKHRSIQLTTQGLELYKTLQDNFT
SLESVISSWQDPKPKRIVIKAALSFATRALLPKIKEMNERYSDYEIVIIPSLEEDEALNTTDYDLLIFHTRLRNHYDNKS
NFSLLREEFMAPVCATSITTKDTVLDDVLQLPRLHPTVDHLDWITWLNNEKYVPHQKVRNTSFHSLDLALSACLSGQGAT
VTDLLLILPELERNFYYCPRHTTLQHSAWVYYIHQHTHSSAINELIEWLQQEAKREIESLKCLAKTHHWGGIINQIN

Specific function: Regulates the expression of the beta-lactamase gene. Represses cephalosporinase (AmpC) in the presence of beta-lactams and induces it in the absence of them [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=303, Percent_Identity=27.7227722772277, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI1786448, Length=290, Percent_Identity=25.1724137931034, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI1788706, Length=297, Percent_Identity=24.5791245791246, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 36689; Mature: 36558

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPYPTLPYSHNGFKAFESVARLMSFTSAANELNVTQSAVSRQVKQLEDDLNASLIIRKH
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
RSIQLTTQGLELYKTLQDNFTSLESVISSWQDPKPKRIVIKAALSFATRALLPKIKEMNE
CCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHH
RYSDYEIVIIPSLEEDEALNTTDYDLLIFHTRLRNHYDNKSNFSLLREEFMAPVCATSIT
HCCCEEEEEECCCCCCCCCCCCCCHHEEHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCC
TKDTVLDDVLQLPRLHPTVDHLDWITWLNNEKYVPHQKVRNTSFHSLDLALSACLSGQGA
CHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCHHHHCCCCHHHHHHHHHHHHCCCCC
TVTDLLLILPELERNFYYCPRHTTLQHSAWVYYIHQHTHSSAINELIEWLQQEAKREIES
HHHHHHHHHHHHHCCEEECCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
LKCLAKTHHWGGIINQIN
HHHHHHHHHHCCHHCCCC
>Mature Secondary Structure 
TPYPTLPYSHNGFKAFESVARLMSFTSAANELNVTQSAVSRQVKQLEDDLNASLIIRKH
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
RSIQLTTQGLELYKTLQDNFTSLESVISSWQDPKPKRIVIKAALSFATRALLPKIKEMNE
CCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHH
RYSDYEIVIIPSLEEDEALNTTDYDLLIFHTRLRNHYDNKSNFSLLREEFMAPVCATSIT
HCCCEEEEEECCCCCCCCCCCCCCHHEEHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCC
TKDTVLDDVLQLPRLHPTVDHLDWITWLNNEKYVPHQKVRNTSFHSLDLALSACLSGQGA
CHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCHHHHCCCCHHHHHHHHHHHHCCCCC
TVTDLLLILPELERNFYYCPRHTTLQHSAWVYYIHQHTHSSAINELIEWLQQEAKREIES
HHHHHHHHHHHHHCCEEECCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
LKCLAKTHHWGGIINQIN
HHHHHHHHHHCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2786868; 1943705 [H]