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The map label for this gene is purA2 [H]

Identifier: 59711669

GI number: 59711669

Start: 1173472

End: 1174728

Strand: Reverse

Name: purA2 [H]

Synonym: VF_1062

Alternate gene names: 59711669

Gene position: 1174728-1173472 (Counterclockwise)

Preceding gene: 59711673

Following gene: 59711665

Centisome position: 40.54

GC content: 43.28

Gene sequence:

>1257_bases
ATGCCGTCTATTGTTGTTGTTGGTGCTAATTGGGGCGATGAAGGTAAAGGTCGCATCGTTGATTTTCTTGCTGCTGAAGC
GGCTGCAAGTATTCGTTTTCAAGGTGGTAATAACGCCGGTCATACTGTTGTAAATGACTTTGGTACCTTTAAGCTTCACC
AACTTCCAAGCGGTATTTTTAACCCAAACTGTACAGCGGTTCTTGGCCCTGGCATGGTTATTAGCCCTGCTGCATTAAGT
GAAGAAATTGCTGAAGTAAAAGCCGCAGGCATCGAAGTTAAAATGCACATTTCTGATCGTGCAACACTTTGTCTTCCAAT
CCATGCACTTGAAGATACATTGGAGGAATTACGTTTAGGTGATGGTGCTTACGGTTCTACTCGTCAAGGTATTGCTCCTG
CATACGGCGACCGTGTTATGAAAAAAGGCATTCTTGTTGGTTGGCTAAAACAACCTGATGTACTTCTTGAGCGTATTAAA
TTCATGCTTGATTGGAAAATGCCACAACTGAAAGCGCTTTATCCTACTTGTGACTTCAGCCAAACCGCTGAAGAAATGAC
AGAATGGCTATTAGAAGTAACTGCTCCTTGGCGTGAATTTATTTGTAATGTTACTGAACCACTAAAAGTAATGCAGAAAA
ACGACGCAAACTTATTGTTTGAAGCACAACTAGGTGCAGGCCGTGATTTAGTTTACGGTGAATACCCATGGACTACGTCT
TCCAATGTTACAGCGGCTTATGCTGGTATCGGCAGTGGCTTACCTGCGTTACGTCCTGAGCGTGTTATTGCTGTGGCGAA
ATCATTCAGTTCTTCGGTTGGTACAGGTACATTAGTTACTGCAATGGAAGAGCAAGATAACTTCCGTGAAGCAGCTAACG
AGTATGGTGCAGTAACAGGTCGTCCACGTGACATGGGTTACTTTGATGCCGTAGCAACACGCAACGGTGTTGAACTGCAA
GCAGCAACGGAAATCGCATTAACAAAAATCGACTGTTTAAGTGGTATGGCAGATCTTAGAATCTGTACTTCTTATGCTGG
TGAGCACACAGAGAACCCTATTTGGCCTCAAACATCAGCATTAAGTCCTGTGTACGAAAAAATGGAAGCATGGGAAGAAG
ATATTACAGGTTGTCGTACTTTTGAAAGCCTACCAAAAGCGGCTCAAGCTTATGTATTACGTATTGAAGAGTTAATGGGA
GTACCAGTAAGCATGGTATCTGTTGGTCCTGAGCGTGAGCAAATGATCATCCGTTAA

Upstream 100 bases:

>100_bases
ATGATATATGGATTTCTTATCAAGTCGATAATTATAATTAATTTCTCATATTGCAAATCTGATATTAGGATAGTGTCTTA
ATCATTAAGGAGTTTGTGTT

Downstream 100 bases:

>100_bases
TCTAATAAACTAATAAAGCCACACATACCTATTTTGGGAATTTTAGGTAGTGTGGCTTTTTATTATGATAGTATTTATTT
GATTAACTATTTAGCTATAA

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase 2; AdSS 2; IMP--aspartate ligase 2 [H]

Number of amino acids: Translated: 418; Mature: 417

Protein sequence:

>418_residues
MPSIVVVGANWGDEGKGRIVDFLAAEAAASIRFQGGNNAGHTVVNDFGTFKLHQLPSGIFNPNCTAVLGPGMVISPAALS
EEIAEVKAAGIEVKMHISDRATLCLPIHALEDTLEELRLGDGAYGSTRQGIAPAYGDRVMKKGILVGWLKQPDVLLERIK
FMLDWKMPQLKALYPTCDFSQTAEEMTEWLLEVTAPWREFICNVTEPLKVMQKNDANLLFEAQLGAGRDLVYGEYPWTTS
SNVTAAYAGIGSGLPALRPERVIAVAKSFSSSVGTGTLVTAMEEQDNFREAANEYGAVTGRPRDMGYFDAVATRNGVELQ
AATEIALTKIDCLSGMADLRICTSYAGEHTENPIWPQTSALSPVYEKMEAWEEDITGCRTFESLPKAAQAYVLRIEELMG
VPVSMVSVGPEREQMIIR

Sequences:

>Translated_418_residues
MPSIVVVGANWGDEGKGRIVDFLAAEAAASIRFQGGNNAGHTVVNDFGTFKLHQLPSGIFNPNCTAVLGPGMVISPAALS
EEIAEVKAAGIEVKMHISDRATLCLPIHALEDTLEELRLGDGAYGSTRQGIAPAYGDRVMKKGILVGWLKQPDVLLERIK
FMLDWKMPQLKALYPTCDFSQTAEEMTEWLLEVTAPWREFICNVTEPLKVMQKNDANLLFEAQLGAGRDLVYGEYPWTTS
SNVTAAYAGIGSGLPALRPERVIAVAKSFSSSVGTGTLVTAMEEQDNFREAANEYGAVTGRPRDMGYFDAVATRNGVELQ
AATEIALTKIDCLSGMADLRICTSYAGEHTENPIWPQTSALSPVYEKMEAWEEDITGCRTFESLPKAAQAYVLRIEELMG
VPVSMVSVGPEREQMIIR
>Mature_417_residues
PSIVVVGANWGDEGKGRIVDFLAAEAAASIRFQGGNNAGHTVVNDFGTFKLHQLPSGIFNPNCTAVLGPGMVISPAALSE
EIAEVKAAGIEVKMHISDRATLCLPIHALEDTLEELRLGDGAYGSTRQGIAPAYGDRVMKKGILVGWLKQPDVLLERIKF
MLDWKMPQLKALYPTCDFSQTAEEMTEWLLEVTAPWREFICNVTEPLKVMQKNDANLLFEAQLGAGRDLVYGEYPWTTSS
NVTAAYAGIGSGLPALRPERVIAVAKSFSSSVGTGTLVTAMEEQDNFREAANEYGAVTGRPRDMGYFDAVATRNGVELQA
ATEIALTKIDCLSGMADLRICTSYAGEHTENPIWPQTSALSPVYEKMEAWEEDITGCRTFESLPKAAQAYVLRIEELMGV
PVSMVSVGPEREQMIIR

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI34577063, Length=435, Percent_Identity=34.4827586206897, Blast_Score=224, Evalue=1e-58,
Organism=Homo sapiens, GI22748717, Length=430, Percent_Identity=33.7209302325581, Blast_Score=209, Evalue=4e-54,
Organism=Homo sapiens, GI40316944, Length=401, Percent_Identity=31.6708229426434, Blast_Score=172, Evalue=4e-43,
Organism=Escherichia coli, GI1790620, Length=428, Percent_Identity=36.4485981308411, Blast_Score=272, Evalue=3e-74,
Organism=Caenorhabditis elegans, GI25146553, Length=433, Percent_Identity=33.0254041570439, Blast_Score=215, Evalue=3e-56,
Organism=Caenorhabditis elegans, GI25146555, Length=433, Percent_Identity=32.7944572748268, Blast_Score=215, Evalue=3e-56,
Organism=Saccharomyces cerevisiae, GI6324109, Length=436, Percent_Identity=30.7339449541284, Blast_Score=210, Evalue=3e-55,
Organism=Drosophila melanogaster, GI21356033, Length=427, Percent_Identity=33.2552693208431, Blast_Score=213, Evalue=2e-55,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 45503; Mature: 45372

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS00107 PROTEIN_KINASE_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSIVVVGANWGDEGKGRIVDFLAAEAAASIRFQGGNNAGHTVVNDFGTFKLHQLPSGIF
CCCEEEEECCCCCCCCCHHHHHHHHHHHHEEEEECCCCCCCCEECCCCCEEEECCCCCCC
NPNCTAVLGPGMVISPAALSEEIAEVKAAGIEVKMHISDRATLCLPIHALEDTLEELRLG
CCCCCEEECCCCEECHHHHHHHHHHHHHCCEEEEEEECCCCEEEEEHHHHHHHHHHHHCC
DGAYGSTRQGIAPAYGDRVMKKGILVGWLKQPDVLLERIKFMLDWKMPQLKALYPTCDFS
CCCCCCCCCCCCHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHCCCCCHHHEECCCCCCH
QTAEEMTEWLLEVTAPWREFICNVTEPLKVMQKNDANLLFEAQLGAGRDLVYGEYPWTTS
HHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHCCCCCEEEEEECCCCCCEEECCCCCCCC
SNVTAAYAGIGSGLPALRPERVIAVAKSFSSSVGTGTLVTAMEEQDNFREAANEYGAVTG
CCCEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHCCCCCC
RPRDMGYFDAVATRNGVELQAATEIALTKIDCLSGMADLRICTSYAGEHTENPIWPQTSA
CCCCCCHHHHHHCCCCCEEEEHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCC
LSPVYEKMEAWEEDITGCRTFESLPKAAQAYVLRIEELMGVPVSMVSVGPEREQMIIR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHCCCCC
>Mature Secondary Structure 
PSIVVVGANWGDEGKGRIVDFLAAEAAASIRFQGGNNAGHTVVNDFGTFKLHQLPSGIF
CCEEEEECCCCCCCCCHHHHHHHHHHHHEEEEECCCCCCCCEECCCCCEEEECCCCCCC
NPNCTAVLGPGMVISPAALSEEIAEVKAAGIEVKMHISDRATLCLPIHALEDTLEELRLG
CCCCCEEECCCCEECHHHHHHHHHHHHHCCEEEEEEECCCCEEEEEHHHHHHHHHHHHCC
DGAYGSTRQGIAPAYGDRVMKKGILVGWLKQPDVLLERIKFMLDWKMPQLKALYPTCDFS
CCCCCCCCCCCCHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHCCCCCHHHEECCCCCCH
QTAEEMTEWLLEVTAPWREFICNVTEPLKVMQKNDANLLFEAQLGAGRDLVYGEYPWTTS
HHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHCCCCCEEEEEECCCCCCEEECCCCCCCC
SNVTAAYAGIGSGLPALRPERVIAVAKSFSSSVGTGTLVTAMEEQDNFREAANEYGAVTG
CCCEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHCCCCCC
RPRDMGYFDAVATRNGVELQAATEIALTKIDCLSGMADLRICTSYAGEHTENPIWPQTSA
CCCCCCHHHHHHCCCCCEEEEHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCC
LSPVYEKMEAWEEDITGCRTFESLPKAAQAYVLRIEELMGVPVSMVSVGPEREQMIIR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA