Click here to switch to the map view.

The map label for this gene is cysH

Identifier: 59710919

GI number: 59710919

Start: 322197

End: 322958

Strand: Direct

Name: cysH

Synonym: VF_0312

Alternate gene names: 59710919

Gene position: 322197-322958 (Clockwise)

Preceding gene: 59710918

Following gene: 59710922

Centisome position: 11.12

GC content: 39.37

Gene sequence:

>762_bases
ATGCCTAAATTGCAATTATCAGAGCTGTTATCGTTAACCAAGGTTGAGCAAACATTACGTTTAGCTGAGGTTAATGTTGA
GCTTGAGAAACTAACAGCCCAAGAGCGTGTTGTTTGGGCGTTGGAAAACTTAGAAGGTAACCCTGCGTTGTCATCCAGTT
TTGGTATTCAAGCTGCGGTGATGTTGCAATTAGTTACTGAAGTAAAATCAGATACCCCTATTATTTTGACTGATACTGGG
TATCTATTTCCTGAAACTTATCAATTTATCGATCAATTAACGGACCGATTGAATTTAAATCTTCATGTCTTTACTGCGGA
TGAGAGCCCAAATTGGCAAGAGGCCCGTTATGGTAAATTATGGGAGCAAGGTGTAGAAGGCATTGAGAAATATAATAAAC
TCAATAAAGTTCAGCCGATGCGTCGTGCTTTAGATCAACTTGAAATTGGAGTTTGGTTTTCAGGGTTACGTCGAGAGCAA
TCGGGTTCTCGAGCAAATTTACCTATTTTAAGTATCCAAAATGGCGTTTTTAAATTCCTCCCAGTCTTAGATTGGACAAA
TAAAGAAGTTCATTACTTTTTGAAAGAGTACGACTTACCTTACCATCCATTATGGGACCAAGGGTATTTATCTGTTGGTG
ATACACACACAACTCAGAAATGGGAACCGGGAATGAGCGAAGAGGAAACTCGATTTTTTGGCTTGAAGCGGGAGTGTGGA
TTACATGAAGATGACGGTGAACTTGATGGTTCTGGAATTTAA

Upstream 100 bases:

>100_bases
GGTCGTTGGGCGACCGAGCGTAATGAAAATGAAGGGTTTGGTGATTTTACGATCCGTGCAGGTATCGTGGATGAAGTGAA
GGTATCAAAGAGGGATTTTC

Downstream 100 bases:

>100_bases
ATGATAAAACTATCATTAAGTAAATTTAATTGGATTAAAAGGCAGCGATAACGCTGCCTTTATTTATCTTGATTGTTTAT
AACTTCATTATTTCCAAAGA

Product: phosphoadenosine phosphosulfate reductase

Products: NA

Alternate protein names: 3'-phosphoadenylylsulfate reductase; PAPS reductase, thioredoxin dependent; PAPS sulfotransferase; PAdoPS reductase

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MPKLQLSELLSLTKVEQTLRLAEVNVELEKLTAQERVVWALENLEGNPALSSSFGIQAAVMLQLVTEVKSDTPIILTDTG
YLFPETYQFIDQLTDRLNLNLHVFTADESPNWQEARYGKLWEQGVEGIEKYNKLNKVQPMRRALDQLEIGVWFSGLRREQ
SGSRANLPILSIQNGVFKFLPVLDWTNKEVHYFLKEYDLPYHPLWDQGYLSVGDTHTTQKWEPGMSEEETRFFGLKRECG
LHEDDGELDGSGI

Sequences:

>Translated_253_residues
MPKLQLSELLSLTKVEQTLRLAEVNVELEKLTAQERVVWALENLEGNPALSSSFGIQAAVMLQLVTEVKSDTPIILTDTG
YLFPETYQFIDQLTDRLNLNLHVFTADESPNWQEARYGKLWEQGVEGIEKYNKLNKVQPMRRALDQLEIGVWFSGLRREQ
SGSRANLPILSIQNGVFKFLPVLDWTNKEVHYFLKEYDLPYHPLWDQGYLSVGDTHTTQKWEPGMSEEETRFFGLKRECG
LHEDDGELDGSGI
>Mature_252_residues
PKLQLSELLSLTKVEQTLRLAEVNVELEKLTAQERVVWALENLEGNPALSSSFGIQAAVMLQLVTEVKSDTPIILTDTGY
LFPETYQFIDQLTDRLNLNLHVFTADESPNWQEARYGKLWEQGVEGIEKYNKLNKVQPMRRALDQLEIGVWFSGLRREQS
GSRANLPILSIQNGVFKFLPVLDWTNKEVHYFLKEYDLPYHPLWDQGYLSVGDTHTTQKWEPGMSEEETRFFGLKRECGL
HEDDGELDGSGI

Specific function: Reduction of activated sulfate into sulfite

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysH subfamily

Homologues:

Organism=Escherichia coli, GI1789121, Length=243, Percent_Identity=69.9588477366255, Blast_Score=363, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6325425, Length=235, Percent_Identity=33.1914893617021, Blast_Score=112, Evalue=6e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CYSH_VIBF1 (Q5E839)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_203695.1
- HSSP:   P17854
- ProteinModelPortal:   Q5E839
- SMR:   Q5E839
- STRING:   Q5E839
- GeneID:   3277928
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_0312
- NMPDR:   fig|312309.3.peg.312
- eggNOG:   COG0175
- HOGENOM:   HBG758022
- OMA:   TNFRPYE
- PhylomeDB:   Q5E839
- ProtClustDB:   PRK02090
- BioCyc:   VFIS312309:VF0312-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00063
- InterPro:   IPR004511
- InterPro:   IPR002500
- InterPro:   IPR011800
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- TIGRFAMs:   TIGR00434
- TIGRFAMs:   TIGR02057

Pfam domain/function: PF01507 PAPS_reduct

EC number: =1.8.4.8

Molecular weight: Translated: 29047; Mature: 28916

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKLQLSELLSLTKVEQTLRLAEVNVELEKLTAQERVVWALENLEGNPALSSSFGIQAAV
CCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHH
MLQLVTEVKSDTPIILTDTGYLFPETYQFIDQLTDRLNLNLHVFTADESPNWQEARYGKL
HHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHH
WEQGVEGIEKYNKLNKVQPMRRALDQLEIGVWFSGLRREQSGSRANLPILSIQNGVFKFL
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHH
PVLDWTNKEVHYFLKEYDLPYHPLWDQGYLSVGDTHTTQKWEPGMSEEETRFFGLKRECG
HHCCCCCHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCHHHHHCCHHHCC
LHEDDGELDGSGI
CCCCCCCCCCCCC
>Mature Secondary Structure 
PKLQLSELLSLTKVEQTLRLAEVNVELEKLTAQERVVWALENLEGNPALSSSFGIQAAV
CCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHH
MLQLVTEVKSDTPIILTDTGYLFPETYQFIDQLTDRLNLNLHVFTADESPNWQEARYGKL
HHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHH
WEQGVEGIEKYNKLNKVQPMRRALDQLEIGVWFSGLRREQSGSRANLPILSIQNGVFKFL
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHH
PVLDWTNKEVHYFLKEYDLPYHPLWDQGYLSVGDTHTTQKWEPGMSEEETRFFGLKRECG
HHCCCCCHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCHHHHHCCHHHCC
LHEDDGELDGSGI
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA