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The map label for this gene is cysI [H]

Identifier: 59710918

GI number: 59710918

Start: 320474

End: 322204

Strand: Direct

Name: cysI [H]

Synonym: VF_0311

Alternate gene names: 59710918

Gene position: 320474-322204 (Clockwise)

Preceding gene: 59710917

Following gene: 59710919

Centisome position: 11.06

GC content: 41.48

Gene sequence:

>1731_bases
ATGAGCGAATTAATTGACGTTAAAACGATTTTATCAGGGACAGAGCTTGGTCCACTTGCTGATAACGAGCGATTAAAGCG
TGAAAGTAAGTATTTACGTGGCACGATCGTAGAAGATCTCCAAGATCGAATTACTGGTGGATTTACCAAAGATAATTTTC
AGCTGATCCGCTTTCATGGCATGTATCAACAAGATGATCGTGATATTCGTGCTGAGCGTGCGAAACAAAAATTAGAACCC
TTGCATAATGTCATGTTACGAGCTCGTATGCCTGGCGGCATTATTACACCAAAGCAGTGGTTGGCGATTGATAAATTTGC
TGAAGAACATACCTCTTATGGCAGTTTGCGCTTAACGACACGTCAAACCTTCCAGTTTCATGGTGTGTTAAAACCAAATA
TTAAATTAATGCATCAAACATTAAACAGTATCGGTATAGATTCAATTGCGACAGCAGGGGATGTAAACCGAAATGTGTTG
TGTACAACTAACCCTGTTGAATCTGAACTTCATCAAGAAGCGTATGAATGGGCGAAAAAAATCAGTGAGCATTTATTACC
AAAAACACGTGCCTACGCTGAAATTTGGTTAGATGGTGAAAAGCTAGAAACCACGGATGAAGAACCGATTTTAGGCAGTA
ATTATCTGCCTCGTAAATTTAAAACCACCGTTGTTATTCCTCCGCAAAATGATGTGGACGTGCATGCGAATGATTTGAAC
TTCATTGCCATTGCAGATAATGGAAAACTGGTTGGCTTTAATGTGCTTGTAGGTGGTGGCCTTGCAATGACACATGGTGA
TACGGCAACTTACCCACGTAAAGCCGATGATTTTGGTTTTGTACCGTTAGATAAAACCCTTGATGTTGCAGCGGCAGTTG
TGACTACTCAGCGTGACTGGGGGAACCGTTCAAATCGTAAAAATGCAAAAACCAAATACACATTAGATCGTGTGGGTTCT
GATGTATTTAAAGGGGAAGTTGAAAAACGAGCTGGCGTTCAGTTTGAAAAAAGTCGTCCCTATGAGTTAACGGAACGTGG
TGATCGAATCGGTTGGGTTGAAGGGATTGATGGTAAGCATCACTTAGCATTATTTATTGAAAATGGGCGTTTATTAGATT
ATCCGGGAAAACCACTAAAAACAGGTGTGGCTGAAATTGCTAAGGTTCACCAAGGTGATTTTAGAATGACAGCAAACCAA
AATTTGATTGTTGCTGGTGTCCCTTCTGAGCAAAAAGATCATATAGAGCAAATTGCTCGTGCGCATGGTTTGATTGATGA
TACTCATTCAGAGCAGCGTAAAAATTCAATGGCTTGTGTGGCGTTTCCTACGTGTCCATTAGCCATGGCGGAAGCCGAGC
GTTTCTTACCTGAATTTGTGACTGAAATTGAAGGTGTATTGAAAAAACACAACCTGCCAGAAGAAGACAACATTATTTTC
CGAGTTACTGGTTGTCCAAATGGTTGTGGCCGAGCAATGTTGGCTGAAATTGGTCTTGTTGGTAAAGCGCCGGGGCGTTA
TAACTTCCATCTTGGTGGTAATCGCAGTGGAACTCGAGTTCCTAAAATGTATAAGGAAAACATCACTGACCGCCAAATCC
TTACTGAAATTGATCAACTTGTTGGTCGTTGGGCGACCGAGCGTAATGAAAATGAAGGGTTTGGTGATTTTACGATCCGT
GCAGGTATCGTGGATGAAGTGAAGGTATCAAAGAGGGATTTTCATGCCTAA

Upstream 100 bases:

>100_bases
TTTGCGTTGTAGAGCAACATGGACAGAAAACGAGAGAAGAAGCAGAGCAATTTGTAAATGAGCTGCGTAAGGCAAAACGT
TATCAAAGGGATGTGTACTA

Downstream 100 bases:

>100_bases
ATTGCAATTATCAGAGCTGTTATCGTTAACCAAGGTTGAGCAAACATTACGTTTAGCTGAGGTTAATGTTGAGCTTGAGA
AACTAACAGCCCAAGAGCGT

Product: sulfite reductase subunit beta

Products: NA

Alternate protein names: SiR-HP; SiRHP [H]

Number of amino acids: Translated: 576; Mature: 575

Protein sequence:

>576_residues
MSELIDVKTILSGTELGPLADNERLKRESKYLRGTIVEDLQDRITGGFTKDNFQLIRFHGMYQQDDRDIRAERAKQKLEP
LHNVMLRARMPGGIITPKQWLAIDKFAEEHTSYGSLRLTTRQTFQFHGVLKPNIKLMHQTLNSIGIDSIATAGDVNRNVL
CTTNPVESELHQEAYEWAKKISEHLLPKTRAYAEIWLDGEKLETTDEEPILGSNYLPRKFKTTVVIPPQNDVDVHANDLN
FIAIADNGKLVGFNVLVGGGLAMTHGDTATYPRKADDFGFVPLDKTLDVAAAVVTTQRDWGNRSNRKNAKTKYTLDRVGS
DVFKGEVEKRAGVQFEKSRPYELTERGDRIGWVEGIDGKHHLALFIENGRLLDYPGKPLKTGVAEIAKVHQGDFRMTANQ
NLIVAGVPSEQKDHIEQIARAHGLIDDTHSEQRKNSMACVAFPTCPLAMAEAERFLPEFVTEIEGVLKKHNLPEEDNIIF
RVTGCPNGCGRAMLAEIGLVGKAPGRYNFHLGGNRSGTRVPKMYKENITDRQILTEIDQLVGRWATERNENEGFGDFTIR
AGIVDEVKVSKRDFHA

Sequences:

>Translated_576_residues
MSELIDVKTILSGTELGPLADNERLKRESKYLRGTIVEDLQDRITGGFTKDNFQLIRFHGMYQQDDRDIRAERAKQKLEP
LHNVMLRARMPGGIITPKQWLAIDKFAEEHTSYGSLRLTTRQTFQFHGVLKPNIKLMHQTLNSIGIDSIATAGDVNRNVL
CTTNPVESELHQEAYEWAKKISEHLLPKTRAYAEIWLDGEKLETTDEEPILGSNYLPRKFKTTVVIPPQNDVDVHANDLN
FIAIADNGKLVGFNVLVGGGLAMTHGDTATYPRKADDFGFVPLDKTLDVAAAVVTTQRDWGNRSNRKNAKTKYTLDRVGS
DVFKGEVEKRAGVQFEKSRPYELTERGDRIGWVEGIDGKHHLALFIENGRLLDYPGKPLKTGVAEIAKVHQGDFRMTANQ
NLIVAGVPSEQKDHIEQIARAHGLIDDTHSEQRKNSMACVAFPTCPLAMAEAERFLPEFVTEIEGVLKKHNLPEEDNIIF
RVTGCPNGCGRAMLAEIGLVGKAPGRYNFHLGGNRSGTRVPKMYKENITDRQILTEIDQLVGRWATERNENEGFGDFTIR
AGIVDEVKVSKRDFHA
>Mature_575_residues
SELIDVKTILSGTELGPLADNERLKRESKYLRGTIVEDLQDRITGGFTKDNFQLIRFHGMYQQDDRDIRAERAKQKLEPL
HNVMLRARMPGGIITPKQWLAIDKFAEEHTSYGSLRLTTRQTFQFHGVLKPNIKLMHQTLNSIGIDSIATAGDVNRNVLC
TTNPVESELHQEAYEWAKKISEHLLPKTRAYAEIWLDGEKLETTDEEPILGSNYLPRKFKTTVVIPPQNDVDVHANDLNF
IAIADNGKLVGFNVLVGGGLAMTHGDTATYPRKADDFGFVPLDKTLDVAAAVVTTQRDWGNRSNRKNAKTKYTLDRVGSD
VFKGEVEKRAGVQFEKSRPYELTERGDRIGWVEGIDGKHHLALFIENGRLLDYPGKPLKTGVAEIAKVHQGDFRMTANQN
LIVAGVPSEQKDHIEQIARAHGLIDDTHSEQRKNSMACVAFPTCPLAMAEAERFLPEFVTEIEGVLKKHNLPEEDNIIFR
VTGCPNGCGRAMLAEIGLVGKAPGRYNFHLGGNRSGTRVPKMYKENITDRQILTEIDQLVGRWATERNENEGFGDFTIRA
GIVDEVKVSKRDFHA

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate [H]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Escherichia coli, GI1789122, Length=558, Percent_Identity=70.9677419354839, Blast_Score=854, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6322597, Length=577, Percent_Identity=44.367417677643, Blast_Score=504, Evalue=1e-143,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011786
- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.8.1.2 [H]

Molecular weight: Translated: 64632; Mature: 64501

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELIDVKTILSGTELGPLADNERLKRESKYLRGTIVEDLQDRITGGFTKDNFQLIRFHG
CCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEC
MYQQDDRDIRAERAKQKLEPLHNVMLRARMPGGIITPKQWLAIDKFAEEHTSYGSLRLTT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEE
RQTFQFHGVLKPNIKLMHQTLNSIGIDSIATAGDVNRNVLCTTNPVESELHQEAYEWAKK
EEEEEEECCCCCCHHHHHHHHHHCCCCHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHH
ISEHLLPKTRAYAEIWLDGEKLETTDEEPILGSNYLPRKFKTTVVIPPQNDVDVHANDLN
HHHHHCCCHHCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEECCEE
FIAIADNGKLVGFNVLVGGGLAMTHGDTATYPRKADDFGFVPLDKTLDVAAAVVTTQRDW
EEEEECCCEEEEEEEEEECCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHEEECCCC
GNRSNRKNAKTKYTLDRVGSDVFKGEVEKRAGVQFEKSRPYELTERGDRIGWVEGIDGKH
CCCCCCCCCCCEEEHHHHCCHHHHHHHHHHCCCEECCCCCEEHHHCCCEEEEEECCCCCE
HLALFIENGRLLDYPGKPLKTGVAEIAKVHQGDFRMTANQNLIVAGVPSEQKDHIEQIAR
EEEEEEECCEEECCCCCHHHHHHHHHHHHCCCCEEEECCCCEEEEECCCCHHHHHHHHHH
AHGLIDDTHSEQRKNSMACVAFPTCPLAMAEAERFLPEFVTEIEGVLKKHNLPEEDNIIF
HCCCCCCCCHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
RVTGCPNGCGRAMLAEIGLVGKAPGRYNFHLGGNRSGTRVPKMYKENITDRQILTEIDQL
EEECCCCCHHHHHHHHHCCEECCCCCEEEEECCCCCCCCCCHHHHHCCCHHHHHHHHHHH
VGRWATERNENEGFGDFTIRAGIVDEVKVSKRDFHA
HHHHHHCCCCCCCCCCEEEEECCHHHHHHCHHCCCC
>Mature Secondary Structure 
SELIDVKTILSGTELGPLADNERLKRESKYLRGTIVEDLQDRITGGFTKDNFQLIRFHG
CCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEC
MYQQDDRDIRAERAKQKLEPLHNVMLRARMPGGIITPKQWLAIDKFAEEHTSYGSLRLTT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEE
RQTFQFHGVLKPNIKLMHQTLNSIGIDSIATAGDVNRNVLCTTNPVESELHQEAYEWAKK
EEEEEEECCCCCCHHHHHHHHHHCCCCHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHH
ISEHLLPKTRAYAEIWLDGEKLETTDEEPILGSNYLPRKFKTTVVIPPQNDVDVHANDLN
HHHHHCCCHHCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEECCEE
FIAIADNGKLVGFNVLVGGGLAMTHGDTATYPRKADDFGFVPLDKTLDVAAAVVTTQRDW
EEEEECCCEEEEEEEEEECCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHEEECCCC
GNRSNRKNAKTKYTLDRVGSDVFKGEVEKRAGVQFEKSRPYELTERGDRIGWVEGIDGKH
CCCCCCCCCCCEEEHHHHCCHHHHHHHHHHCCCEECCCCCEEHHHCCCEEEEEECCCCCE
HLALFIENGRLLDYPGKPLKTGVAEIAKVHQGDFRMTANQNLIVAGVPSEQKDHIEQIAR
EEEEEEECCEEECCCCCHHHHHHHHHHHHCCCCEEEECCCCEEEEECCCCHHHHHHHHHH
AHGLIDDTHSEQRKNSMACVAFPTCPLAMAEAERFLPEFVTEIEGVLKKHNLPEEDNIIF
HCCCCCCCCHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
RVTGCPNGCGRAMLAEIGLVGKAPGRYNFHLGGNRSGTRVPKMYKENITDRQILTEIDQL
EEECCCCCHHHHHHHHHCCEECCCCCEEEEECCCCCCCCCCHHHHHCCCHHHHHHHHHHH
VGRWATERNENEGFGDFTIRAGIVDEVKVSKRDFHA
HHHHHHCCCCCCCCCCEEEEECCHHHHHHCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA