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The map label for this gene is dusA [H]

Identifier: 59710914

GI number: 59710914

Start: 316316

End: 317302

Strand: Direct

Name: dusA [H]

Synonym: VF_0307

Alternate gene names: 59710914

Gene position: 316316-317302 (Clockwise)

Preceding gene: 59710911

Following gene: 59710915

Centisome position: 10.92

GC content: 40.73

Gene sequence:

>987_bases
ATGAGCTCTTATCCACTACAACGTTTTTCTGTTGCCCCTATGCTTGATTGGACAGATCGTCACTGTCGTTACTTCCATCG
TCAATTATCTGAGCATGCGCTTTTATATACTGAGATGGTAACTACAGGTGCGATCATTCATGGTAAAGGTGATTTTCTTG
CGTATAACGAAGAAGAGCATCCAGTAGCTTTGCAGCTAGGCGGTTCAAACGTTAAAGATTTAGTTCATTGTGCGAAATTA
GCAGCAGAACGTGGTTACGATGAAGTAAACCTAAATGTGGGCTGTCCATCAGATCGAGTACAAAATGGCCGTTTTGGTGC
TTGTTTAATGGCAGAACCAGACTTAGTAGCTGAATGTGTCGCTGAAATGAAAGCGGTTGTTGATATTCCAGTAACGGTAA
AAACCCGTATTGGTATTGATGACCAAGACAGCTATGAGTTTTTAACTAAGTTTGTTTCAACAGTTCATGAAAAGGGCGGT
TGTGATCAATTTACTATTCATGCTCGTAAAGCATGGTTAAGTGGTTTAAGCCCGAAAGAAAACCGCGAGATCCCGCCACT
TGATTACCCACGTGCGTATCAAATTAAGAAAGATTTCCCACAATTAATTATTGCGGTTAATGGTGGTATTAAAACATTAT
CGGAAGCAAAAGAGCATTTAGAGCATTTAGACGGTGTGATGATTGGACGTGAGGCTTACCAAAGTCCGTATTTATTAGCG
GAAGTTGATCAACAACTTTTTGGTAGTGACAAACCAATTAAAAAGCGTCGTGAGATTGTTGAGTCTATGTACCCATATAT
TGAACGTCAGCTAGAGAATGGAGCTTACTTAGGCCATATTACTCGTCATATGTTAGGTCTATTCCAAAGCATGCCGGGTG
CGAGACAATGGCGTCGTTATATCAGCGAAAATGCGCATAAGCCAGGAGCAGGCATTGAAGTGGTAAAAACAGCACTTGCT
AAAATTCCTTCTGAATTAGATGTATAG

Upstream 100 bases:

>100_bases
TATTATTTGTTATGTGAGTTGCCTAATAGAGTTTATGGGATGGTTGTTTGTGTTCTTATTATGTTAGAATTGAGGCCTTG
ATCACTGAAAGAATTAACCT

Downstream 100 bases:

>100_bases
ATTTTAGCCACTCTGTCGATAAATTAAGTTAGATACGTAAAAGAAGAGTATATAAATAAACCAAATCGTTTATTATTACT
TCATATGAACCAAATAGCGT

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MSSYPLQRFSVAPMLDWTDRHCRYFHRQLSEHALLYTEMVTTGAIIHGKGDFLAYNEEEHPVALQLGGSNVKDLVHCAKL
AAERGYDEVNLNVGCPSDRVQNGRFGACLMAEPDLVAECVAEMKAVVDIPVTVKTRIGIDDQDSYEFLTKFVSTVHEKGG
CDQFTIHARKAWLSGLSPKENREIPPLDYPRAYQIKKDFPQLIIAVNGGIKTLSEAKEHLEHLDGVMIGREAYQSPYLLA
EVDQQLFGSDKPIKKRREIVESMYPYIERQLENGAYLGHITRHMLGLFQSMPGARQWRRYISENAHKPGAGIEVVKTALA
KIPSELDV

Sequences:

>Translated_328_residues
MSSYPLQRFSVAPMLDWTDRHCRYFHRQLSEHALLYTEMVTTGAIIHGKGDFLAYNEEEHPVALQLGGSNVKDLVHCAKL
AAERGYDEVNLNVGCPSDRVQNGRFGACLMAEPDLVAECVAEMKAVVDIPVTVKTRIGIDDQDSYEFLTKFVSTVHEKGG
CDQFTIHARKAWLSGLSPKENREIPPLDYPRAYQIKKDFPQLIIAVNGGIKTLSEAKEHLEHLDGVMIGREAYQSPYLLA
EVDQQLFGSDKPIKKRREIVESMYPYIERQLENGAYLGHITRHMLGLFQSMPGARQWRRYISENAHKPGAGIEVVKTALA
KIPSELDV
>Mature_327_residues
SSYPLQRFSVAPMLDWTDRHCRYFHRQLSEHALLYTEMVTTGAIIHGKGDFLAYNEEEHPVALQLGGSNVKDLVHCAKLA
AERGYDEVNLNVGCPSDRVQNGRFGACLMAEPDLVAECVAEMKAVVDIPVTVKTRIGIDDQDSYEFLTKFVSTVHEKGGC
DQFTIHARKAWLSGLSPKENREIPPLDYPRAYQIKKDFPQLIIAVNGGIKTLSEAKEHLEHLDGVMIGREAYQSPYLLAE
VDQQLFGSDKPIKKRREIVESMYPYIERQLENGAYLGHITRHMLGLFQSMPGARQWRRYISENAHKPGAGIEVVKTALAK
IPSELDV

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=296, Percent_Identity=24.3243243243243, Blast_Score=75, Evalue=9e-14,
Organism=Escherichia coli, GI145693211, Length=312, Percent_Identity=73.3974358974359, Blast_Score=494, Evalue=1e-141,
Organism=Escherichia coli, GI1789660, Length=246, Percent_Identity=26.4227642276423, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1788462, Length=311, Percent_Identity=25.7234726688103, Blast_Score=65, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17507177, Length=168, Percent_Identity=26.7857142857143, Blast_Score=71, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI25144369, Length=232, Percent_Identity=27.1551724137931, Blast_Score=67, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24580595, Length=250, Percent_Identity=26, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19920448, Length=250, Percent_Identity=26, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 36955; Mature: 36824

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSYPLQRFSVAPMLDWTDRHCRYFHRQLSEHALLYTEMVTTGAIIHGKGDFLAYNEEEH
CCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCEEEECCCCC
PVALQLGGSNVKDLVHCAKLAAERGYDEVNLNVGCPSDRVQNGRFGACLMAEPDLVAECV
CEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHCCCCCCEEEECCHHHHHHHH
AEMKAVVDIPVTVKTRIGIDDQDSYEFLTKFVSTVHEKGGCDQFTIHARKAWLSGLSPKE
HHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHCCCCCCC
NREIPPLDYPRAYQIKKDFPQLIIAVNGGIKTLSEAKEHLEHLDGVMIGREAYQSPYLLA
CCCCCCCCCCCCHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCHHHHCCCCHHH
EVDQQLFGSDKPIKKRREIVESMYPYIERQLENGAYLGHITRHMLGLFQSMPGARQWRRY
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHH
ISENAHKPGAGIEVVKTALAKIPSELDV
HHHCCCCCCCCHHHHHHHHHHCCHHCCC
>Mature Secondary Structure 
SSYPLQRFSVAPMLDWTDRHCRYFHRQLSEHALLYTEMVTTGAIIHGKGDFLAYNEEEH
CCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCEEEECCCCC
PVALQLGGSNVKDLVHCAKLAAERGYDEVNLNVGCPSDRVQNGRFGACLMAEPDLVAECV
CEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHCCCCCCEEEECCHHHHHHHH
AEMKAVVDIPVTVKTRIGIDDQDSYEFLTKFVSTVHEKGGCDQFTIHARKAWLSGLSPKE
HHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHCCCCCCC
NREIPPLDYPRAYQIKKDFPQLIIAVNGGIKTLSEAKEHLEHLDGVMIGREAYQSPYLLA
CCCCCCCCCCCCHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCHHHHCCCCHHH
EVDQQLFGSDKPIKKRREIVESMYPYIERQLENGAYLGHITRHMLGLFQSMPGARQWRRY
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHH
ISENAHKPGAGIEVVKTALAKIPSELDV
HHHCCCCCCCCHHHHHHHHHHCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]