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The map label for this gene is leuC [H]

Identifier: 59710900

GI number: 59710900

Start: 298584

End: 299999

Strand: Reverse

Name: leuC [H]

Synonym: VF_0293

Alternate gene names: 59710900

Gene position: 299999-298584 (Counterclockwise)

Preceding gene: 59710901

Following gene: 59710899

Centisome position: 10.35

GC content: 45.06

Gene sequence:

>1416_bases
ATGTCTAACGCAAAAACATTATACGAAAAAATTTACGATGCCCATGTTGCCGTTGCAGCTGAAGGCGAAAACCCTATTTT
ATACATCGACCGCCATCTGGTTCACGAAGTAACGTCACCACAAGCGTTCGATGGTCTACGTGAAAAAGGACGTAAAGTTC
GTCAAGTAGGCAAAACCTTTGCAACGATGGATCACAACGTTTCAACACAAACCAAAGACATTAATGCCTCTGGTGAAATG
GCTCGAATCCAAATGGAAACGCTGTCTAAAAACTGTGAAGAATTTGGTGTTACCCTTTACGATCTAAATCATAAATACCA
AGGTATTGTGCACGTAATGGGACCAGAACTTGGTATTACTTTACCAGGTATGACGATTGTTTGTGGTGACTCACACACCG
CAACTCACGGTGCATTTGGTTCACTAGCATTCGGTATCGGTACTTCAGAAGTTGAGCACGTACTAGCAACACAAACGCTA
AAACAAGCTCGTGCGAAAACCATGAAAATCGATGTTAAAGGTAAAGTCGCTGAAGGCATCACGGCAAAAGATATCGTGCT
AGCAATCATTGGTAAAACAACGGCTGCTGGTGGTACGGGCTACGTAGTTGAGTTCTGTGGCGAAGCAATTACTGACCTTA
CTATGGAAGGTCGTATGACAGTGTGTAACATGGCTATTGAGCTAGGTGCTAAAGCGGGTCTAATCGCACCAGACCAAACC
ACATTTGATTACATTGCGGGTCGTAAGTTCTCACCACAAGATGCAGATTTAGACGCCGCTATCGAATACTGGTCATCATT
GAAAACGGATGATGATGCTGAGTTTGATGCAGTCGTTACGCTTGATGCCTCTGAAATTAAACCGCAAGTAACTTGGGGAA
CCAACCCAGGCCAAGTAATCGCAGTAGATGCACCAATCCCAGCACCAGAAAGCTTTGCTGACCCTGTAGAAAAAGCGTCT
GCAGAAAAAGCCTTAGCGTATATGGGTCTTGAAGCGGGTAAATCTCTGTCTGATTACAACGTTGATAAAGTTTTTGTTGG
CTCTTGTACTAACTCGCGAATTGAAGACATGCGTGCTGCGGCAGCCATTGCTAAAGGACGTAAAGTTGCCTCTCATGTAC
AAGCTCTAATTGTTCCAGGCTCTGAGCAAGTAAAAGCACAAGCGGAAAAAGAAGGTCTAGATGTGATCTTTAAAGAAGCA
GGCTTTGAATGGCGTCTGCCAGGTTGCTCTATGTGTCTTGCAATGAACAACGACCGCCTAGGTCCACATGAGCGTTGTGC
ATCTACATCAAACCGTAACTTTGAAGGTCGTCAAGGCCGTGATGGTCGAACTCACCTAGTGAGCCCTGCGATGGCTGCCG
CTGCTGCAATTGCAGGTCACTTTGTTGATATCCGCACCATTACAGAACAAGCATAA

Upstream 100 bases:

>100_bases
AACGGCTGACTTAGCCGGTGATAAACCAGCACTAACTACATCAGCAATGGGCGATAAAATCGCTGAGTACATTTTGAATT
CGTAATTCAAGGAAGAAGCA

Downstream 100 bases:

>100_bases
AAGGAATTTACTATGTCAGGTTTTAAGCAACATACAGGTCTAGTCGTTCCTTTAGATACGGCCAACATTGATACCGATGC
CATCATACCAAAGCAGTTTC

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MSNAKTLYEKIYDAHVAVAAEGENPILYIDRHLVHEVTSPQAFDGLREKGRKVRQVGKTFATMDHNVSTQTKDINASGEM
ARIQMETLSKNCEEFGVTLYDLNHKYQGIVHVMGPELGITLPGMTIVCGDSHTATHGAFGSLAFGIGTSEVEHVLATQTL
KQARAKTMKIDVKGKVAEGITAKDIVLAIIGKTTAAGGTGYVVEFCGEAITDLTMEGRMTVCNMAIELGAKAGLIAPDQT
TFDYIAGRKFSPQDADLDAAIEYWSSLKTDDDAEFDAVVTLDASEIKPQVTWGTNPGQVIAVDAPIPAPESFADPVEKAS
AEKALAYMGLEAGKSLSDYNVDKVFVGSCTNSRIEDMRAAAAIAKGRKVASHVQALIVPGSEQVKAQAEKEGLDVIFKEA
GFEWRLPGCSMCLAMNNDRLGPHERCASTSNRNFEGRQGRDGRTHLVSPAMAAAAAIAGHFVDIRTITEQA

Sequences:

>Translated_471_residues
MSNAKTLYEKIYDAHVAVAAEGENPILYIDRHLVHEVTSPQAFDGLREKGRKVRQVGKTFATMDHNVSTQTKDINASGEM
ARIQMETLSKNCEEFGVTLYDLNHKYQGIVHVMGPELGITLPGMTIVCGDSHTATHGAFGSLAFGIGTSEVEHVLATQTL
KQARAKTMKIDVKGKVAEGITAKDIVLAIIGKTTAAGGTGYVVEFCGEAITDLTMEGRMTVCNMAIELGAKAGLIAPDQT
TFDYIAGRKFSPQDADLDAAIEYWSSLKTDDDAEFDAVVTLDASEIKPQVTWGTNPGQVIAVDAPIPAPESFADPVEKAS
AEKALAYMGLEAGKSLSDYNVDKVFVGSCTNSRIEDMRAAAAIAKGRKVASHVQALIVPGSEQVKAQAEKEGLDVIFKEA
GFEWRLPGCSMCLAMNNDRLGPHERCASTSNRNFEGRQGRDGRTHLVSPAMAAAAAIAGHFVDIRTITEQA
>Mature_470_residues
SNAKTLYEKIYDAHVAVAAEGENPILYIDRHLVHEVTSPQAFDGLREKGRKVRQVGKTFATMDHNVSTQTKDINASGEMA
RIQMETLSKNCEEFGVTLYDLNHKYQGIVHVMGPELGITLPGMTIVCGDSHTATHGAFGSLAFGIGTSEVEHVLATQTLK
QARAKTMKIDVKGKVAEGITAKDIVLAIIGKTTAAGGTGYVVEFCGEAITDLTMEGRMTVCNMAIELGAKAGLIAPDQTT
FDYIAGRKFSPQDADLDAAIEYWSSLKTDDDAEFDAVVTLDASEIKPQVTWGTNPGQVIAVDAPIPAPESFADPVEKASA
EKALAYMGLEAGKSLSDYNVDKVFVGSCTNSRIEDMRAAAAIAKGRKVASHVQALIVPGSEQVKAQAEKEGLDVIFKEAG
FEWRLPGCSMCLAMNNDRLGPHERCASTSNRNFEGRQGRDGRTHLVSPAMAAAAAIAGHFVDIRTITEQA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=411, Percent_Identity=27.4939172749392, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI8659555, Length=367, Percent_Identity=27.2479564032698, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI41352693, Length=377, Percent_Identity=27.3209549071618, Blast_Score=98, Evalue=2e-20,
Organism=Escherichia coli, GI1786259, Length=464, Percent_Identity=79.9568965517241, Blast_Score=766, Evalue=0.0,
Organism=Escherichia coli, GI1787531, Length=364, Percent_Identity=27.7472527472527, Blast_Score=98, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI25149337, Length=484, Percent_Identity=26.6528925619835, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI32564738, Length=420, Percent_Identity=27.1428571428571, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI25149342, Length=315, Percent_Identity=28.5714285714286, Blast_Score=125, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI17568399, Length=407, Percent_Identity=26.7813267813268, Blast_Score=102, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6321429, Length=468, Percent_Identity=58.5470085470086, Blast_Score=561, Evalue=1e-161,
Organism=Saccharomyces cerevisiae, GI6323335, Length=478, Percent_Identity=28.6610878661088, Blast_Score=150, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6322261, Length=405, Percent_Identity=26.9135802469136, Blast_Score=145, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6320440, Length=434, Percent_Identity=25.1152073732719, Blast_Score=129, Evalue=8e-31,
Organism=Drosophila melanogaster, GI281365315, Length=479, Percent_Identity=25.2609603340292, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI17864292, Length=479, Percent_Identity=25.2609603340292, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI161076999, Length=419, Percent_Identity=25.5369928400955, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI28571643, Length=482, Percent_Identity=25.3112033195021, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24645686, Length=375, Percent_Identity=27.2, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI17137564, Length=480, Percent_Identity=25.4166666666667, Blast_Score=98, Evalue=1e-20,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 50471; Mature: 50340

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNAKTLYEKIYDAHVAVAAEGENPILYIDRHLVHEVTSPQAFDGLREKGRKVRQVGKTF
CCCHHHHHHHHHHCEEEEEECCCCCEEEECHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
ATMDHNVSTQTKDINASGEMARIQMETLSKNCEEFGVTLYDLNHKYQGIVHVMGPELGIT
HHCCCCCCCCCCCCCCCCCEEEEEHHHHHCCHHHHCCEEEECCCCCCEEEEEECCCCCEE
LPGMTIVCGDSHTATHGAFGSLAFGIGTSEVEHVLATQTLKQARAKTMKIDVKGKVAEGI
CCCEEEEECCCCCCCCCCCHHHEECCCHHHHHHHHHHHHHHHHHHHEEEEEECCCHHCCC
TAKDIVLAIIGKTTAAGGTGYVVEFCGEAITDLTMEGRMTVCNMAIELGAKAGLIAPDQT
CHHHEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCC
TFDYIAGRKFSPQDADLDAAIEYWSSLKTDDDAEFDAVVTLDASEIKPQVTWGTNPGQVI
HHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEEEHHHCCCEEEECCCCCEEE
AVDAPIPAPESFADPVEKASAEKALAYMGLEAGKSLSDYNVDKVFVGSCTNSRIEDMRAA
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHH
AAIAKGRKVASHVQALIVPGSEQVKAQAEKEGLDVIFKEAGFEWRLPGCSMCLAMNNDRL
HHHHHCHHHHHHHEEEEECCCHHHHHHHHHCCCEEEEECCCCEEECCCCEEEEEECCCCC
GPHERCASTSNRNFEGRQGRDGRTHLVSPAMAAAAAIAGHFVDIRTITEQA
CHHHHHCCCCCCCCCCCCCCCCCCEECCHHHHHHHHHHHHHEEEEECCCCC
>Mature Secondary Structure 
SNAKTLYEKIYDAHVAVAAEGENPILYIDRHLVHEVTSPQAFDGLREKGRKVRQVGKTF
CCHHHHHHHHHHCEEEEEECCCCCEEEECHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
ATMDHNVSTQTKDINASGEMARIQMETLSKNCEEFGVTLYDLNHKYQGIVHVMGPELGIT
HHCCCCCCCCCCCCCCCCCEEEEEHHHHHCCHHHHCCEEEECCCCCCEEEEEECCCCCEE
LPGMTIVCGDSHTATHGAFGSLAFGIGTSEVEHVLATQTLKQARAKTMKIDVKGKVAEGI
CCCEEEEECCCCCCCCCCCHHHEECCCHHHHHHHHHHHHHHHHHHHEEEEEECCCHHCCC
TAKDIVLAIIGKTTAAGGTGYVVEFCGEAITDLTMEGRMTVCNMAIELGAKAGLIAPDQT
CHHHEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCC
TFDYIAGRKFSPQDADLDAAIEYWSSLKTDDDAEFDAVVTLDASEIKPQVTWGTNPGQVI
HHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEEEHHHCCCEEEECCCCCEEE
AVDAPIPAPESFADPVEKASAEKALAYMGLEAGKSLSDYNVDKVFVGSCTNSRIEDMRAA
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHH
AAIAKGRKVASHVQALIVPGSEQVKAQAEKEGLDVIFKEAGFEWRLPGCSMCLAMNNDRL
HHHHHCHHHHHHHEEEEECCCHHHHHHHHHCCCEEEEECCCCEEECCCCEEEEEECCCCC
GPHERCASTSNRNFEGRQGRDGRTHLVSPAMAAAAAIAGHFVDIRTITEQA
CHHHHHCCCCCCCCCCCCCCCCCCEECCHHHHHHHHHHHHHEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA