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The map label for this gene is leuB

Identifier: 59710901

GI number: 59710901

Start: 300015

End: 301106

Strand: Reverse

Name: leuB

Synonym: VF_0294

Alternate gene names: 59710901

Gene position: 301106-300015 (Counterclockwise)

Preceding gene: 59710902

Following gene: 59710900

Centisome position: 10.39

GC content: 42.31

Gene sequence:

>1092_bases
ATGACAAACAAAACATACAAAATTGCGGTTCTACCAGGTGATGGCATTGGTCCTGAAGTAATGGAACAAGCACATAAAGT
TCTGGATGCAATTGAGAAAAAACACGCAATTTCATTTTCACGTGAGCAACATGATGTTGGCGGTATTGCCATTGATAACC
ACGGTTGCCCACTTCCTGAAAGCACAGTGAAAGGCTGTGAAGAATCTGATGCGGTATTATTTGGTTCTGTTGGTGGCCCA
AAATGGGAACACCTTCCACCAAATGACCAACCAGAGCGTGGTGCTCTACTGCCTCTTCGTAAGCACTTCCAACTGTTCTG
TAATCTTCGTCCTGCACAGATCCACAAAGGATTAGAAAACTTCTCTCCTTTACGTGCTGATATTTCTGAACGTGGTTTTG
ATATCGTCGTTGTTCGTGAACTGACGGGTGGTATCTACTTTGGTCAACCAAAAGGCCGTGAAGGTGAAGGTCCTCAAGAG
AAAGCGTACGATACTGAAATTTATCATCGTTATGAGATTGAACGCATTGCAAAAATTGCTTTTGAATCTGCTCGTCTACG
TAATAAAAACGTTTACTCTATTGATAAAGCTAACGTTCTACAAAGCTCTATTTTATGGCGTGAAGTAGTAGAAGAAGTGG
CTAAAGGCTACCCTGATGTAACTCTAAATCATATGTACATTGATAACGCGACCATGCAGCTAATCAAAGATCCATCTCAG
TTTGATGTAATGCTATGTTCAAACATCTTTGGTGACATCATCTCAGATGAGTGTGCAATGATCACAGGTTCAATGGGCAT
GCTTCCTTCTGCTAGTTTGAATGAGAGCAACTTTGGTCTGTATGAACCTGCGGGTGGTTCTGCGCCTGATATTGCAGGTA
AAAATATTGCTAACCCTGTTGCTCAGATCTTATCTGCAGCACTCATGTTACGCTATAGCCTAGGTGAAGAAGAAGCAGCA
CAAAGTATTGAAGCTGCCGTATCTAAAGCGCTATCAGCAGGAGAGCTAACGGCTGACTTAGCCGGTGATAAACCAGCACT
AACTACATCAGCAATGGGCGATAAAATCGCTGAGTACATTTTGAATTCGTAA

Upstream 100 bases:

>100_bases
AAAAGTAGAAACCGTTTAGAACCTAAAAATTATAAATAAGCGGAGATAAACCTTTCTCCGCTTTAATTTGCCAAATTATA
TTGAAGATTAAAGGATTAAC

Downstream 100 bases:

>100_bases
TTCAAGGAAGAAGCAATGTCTAACGCAAAAACATTATACGAAAAAATTTACGATGCCCATGTTGCCGTTGCAGCTGAAGG
CGAAAACCCTATTTTATACA

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MTNKTYKIAVLPGDGIGPEVMEQAHKVLDAIEKKHAISFSREQHDVGGIAIDNHGCPLPESTVKGCEESDAVLFGSVGGP
KWEHLPPNDQPERGALLPLRKHFQLFCNLRPAQIHKGLENFSPLRADISERGFDIVVVRELTGGIYFGQPKGREGEGPQE
KAYDTEIYHRYEIERIAKIAFESARLRNKNVYSIDKANVLQSSILWREVVEEVAKGYPDVTLNHMYIDNATMQLIKDPSQ
FDVMLCSNIFGDIISDECAMITGSMGMLPSASLNESNFGLYEPAGGSAPDIAGKNIANPVAQILSAALMLRYSLGEEEAA
QSIEAAVSKALSAGELTADLAGDKPALTTSAMGDKIAEYILNS

Sequences:

>Translated_363_residues
MTNKTYKIAVLPGDGIGPEVMEQAHKVLDAIEKKHAISFSREQHDVGGIAIDNHGCPLPESTVKGCEESDAVLFGSVGGP
KWEHLPPNDQPERGALLPLRKHFQLFCNLRPAQIHKGLENFSPLRADISERGFDIVVVRELTGGIYFGQPKGREGEGPQE
KAYDTEIYHRYEIERIAKIAFESARLRNKNVYSIDKANVLQSSILWREVVEEVAKGYPDVTLNHMYIDNATMQLIKDPSQ
FDVMLCSNIFGDIISDECAMITGSMGMLPSASLNESNFGLYEPAGGSAPDIAGKNIANPVAQILSAALMLRYSLGEEEAA
QSIEAAVSKALSAGELTADLAGDKPALTTSAMGDKIAEYILNS
>Mature_362_residues
TNKTYKIAVLPGDGIGPEVMEQAHKVLDAIEKKHAISFSREQHDVGGIAIDNHGCPLPESTVKGCEESDAVLFGSVGGPK
WEHLPPNDQPERGALLPLRKHFQLFCNLRPAQIHKGLENFSPLRADISERGFDIVVVRELTGGIYFGQPKGREGEGPQEK
AYDTEIYHRYEIERIAKIAFESARLRNKNVYSIDKANVLQSSILWREVVEEVAKGYPDVTLNHMYIDNATMQLIKDPSQF
DVMLCSNIFGDIISDECAMITGSMGMLPSASLNESNFGLYEPAGGSAPDIAGKNIANPVAQILSAALMLRYSLGEEEAAQ
SIEAAVSKALSAGELTADLAGDKPALTTSAMGDKIAEYILNS

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5031777, Length=346, Percent_Identity=33.8150289017341, Blast_Score=165, Evalue=5e-41,
Organism=Homo sapiens, GI28178816, Length=344, Percent_Identity=28.7790697674419, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI28178821, Length=344, Percent_Identity=28.7790697674419, Blast_Score=125, Evalue=6e-29,
Organism=Homo sapiens, GI4758582, Length=362, Percent_Identity=29.2817679558011, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI28178838, Length=332, Percent_Identity=29.8192771084337, Blast_Score=118, Evalue=7e-27,
Organism=Homo sapiens, GI28178819, Length=181, Percent_Identity=34.2541436464088, Blast_Score=100, Evalue=1e-21,
Organism=Escherichia coli, GI87081683, Length=358, Percent_Identity=74.0223463687151, Blast_Score=557, Evalue=1e-160,
Organism=Escherichia coli, GI1788101, Length=369, Percent_Identity=37.1273712737127, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI1787381, Length=400, Percent_Identity=25.75, Blast_Score=83, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71986051, Length=339, Percent_Identity=32.1533923303835, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17550882, Length=349, Percent_Identity=30.6590257879656, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17505779, Length=353, Percent_Identity=30.5949008498584, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI25144293, Length=344, Percent_Identity=29.6511627906977, Blast_Score=124, Evalue=9e-29,
Organism=Saccharomyces cerevisiae, GI6319830, Length=365, Percent_Identity=46.027397260274, Blast_Score=285, Evalue=7e-78,
Organism=Saccharomyces cerevisiae, GI6322097, Length=352, Percent_Identity=36.0795454545455, Blast_Score=171, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6324709, Length=358, Percent_Identity=31.5642458100559, Blast_Score=144, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6324291, Length=344, Percent_Identity=29.9418604651163, Blast_Score=133, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24643268, Length=341, Percent_Identity=31.9648093841642, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI24643270, Length=341, Percent_Identity=31.9648093841642, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI24661184, Length=344, Percent_Identity=30.5232558139535, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI161078633, Length=350, Percent_Identity=29.4285714285714, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24650122, Length=350, Percent_Identity=29.4285714285714, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI161078637, Length=350, Percent_Identity=29.4285714285714, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI161078635, Length=350, Percent_Identity=29.4285714285714, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI161078639, Length=346, Percent_Identity=29.7687861271676, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI281362242, Length=344, Percent_Identity=26.7441860465116, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24648872, Length=344, Percent_Identity=26.7441860465116, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI20130355, Length=350, Percent_Identity=24, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU3_VIBF1 (Q5E857)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_203677.1
- ProteinModelPortal:   Q5E857
- SMR:   Q5E857
- STRING:   Q5E857
- GeneID:   3277254
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_0294
- NMPDR:   fig|312309.3.peg.294
- eggNOG:   COG0473
- HOGENOM:   HBG518924
- OMA:   MSYQIAV
- PhylomeDB:   Q5E857
- ProtClustDB:   PRK00772
- BioCyc:   VFIS312309:VF0294-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01033
- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429
- Gene3D:   G3DSA:3.40.718.10
- PANTHER:   PTHR11835
- PANTHER:   PTHR11835:SF13
- TIGRFAMs:   TIGR00169

Pfam domain/function: PF00180 Iso_dh

EC number: =1.1.1.85

Molecular weight: Translated: 39490; Mature: 39359

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00470 IDH_IMDH

Important sites: BINDING 100-100 BINDING 110-110 BINDING 139-139 BINDING 228-228

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNKTYKIAVLPGDGIGPEVMEQAHKVLDAIEKKHAISFSREQHDVGGIAIDNHGCPLPE
CCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCH
STVKGCEESDAVLFGSVGGPKWEHLPPNDQPERGALLPLRKHFQLFCNLRPAQIHKGLEN
HHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHH
FSPLRADISERGFDIVVVRELTGGIYFGQPKGREGEGPQEKAYDTEIYHRYEIERIAKIA
CCHHHHHHHHCCCEEEEEEECCCCEEECCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHH
FESARLRNKNVYSIDKANVLQSSILWREVVEEVAKGYPDVTLNHMYIDNATMQLIKDPSQ
HHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHCCCCH
FDVMLCSNIFGDIISDECAMITGSMGMLPSASLNESNFGLYEPAGGSAPDIAGKNIANPV
HHHHHHHHHHHHHHCCCHHEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
AQILSAALMLRYSLGEEEAAQSIEAAVSKALSAGELTADLAGDKPALTTSAMGDKIAEYI
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCHHHHHHHHHHHHH
LNS
HCC
>Mature Secondary Structure 
TNKTYKIAVLPGDGIGPEVMEQAHKVLDAIEKKHAISFSREQHDVGGIAIDNHGCPLPE
CCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCH
STVKGCEESDAVLFGSVGGPKWEHLPPNDQPERGALLPLRKHFQLFCNLRPAQIHKGLEN
HHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHH
FSPLRADISERGFDIVVVRELTGGIYFGQPKGREGEGPQEKAYDTEIYHRYEIERIAKIA
CCHHHHHHHHCCCEEEEEEECCCCEEECCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHH
FESARLRNKNVYSIDKANVLQSSILWREVVEEVAKGYPDVTLNHMYIDNATMQLIKDPSQ
HHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHCCCCH
FDVMLCSNIFGDIISDECAMITGSMGMLPSASLNESNFGLYEPAGGSAPDIAGKNIANPV
HHHHHHHHHHHHHHCCCHHEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
AQILSAALMLRYSLGEEEAAQSIEAAVSKALSAGELTADLAGDKPALTTSAMGDKIAEYI
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCHHHHHHHHHHHHH
LNS
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA