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The map label for this gene is leuD [H]

Identifier: 59710899

GI number: 59710899

Start: 297969

End: 298571

Strand: Reverse

Name: leuD [H]

Synonym: VF_0292

Alternate gene names: 59710899

Gene position: 298571-297969 (Counterclockwise)

Preceding gene: 59710900

Following gene: 59710898

Centisome position: 10.3

GC content: 41.63

Gene sequence:

>603_bases
ATGTCAGGTTTTAAGCAACATACAGGTCTAGTCGTTCCTTTAGATACGGCCAACATTGATACCGATGCCATCATACCAAA
GCAGTTTCTACAAAAAGTGAATCGTATTGGTTTTGGTAAACACCTATTTCACGATTGGCGTTTTCTTGATGATGCTGGTG
AGCAACCCAATCCTGAATTTGTTATGAACGCACCTCGCTATCAAGGTGCTACCGTTCTACTTGCTCGTGAAAACTTTGGT
TGTGGTTCTTCTCGTGAACACGCACCTTGGTCACTAGCGGACTACGGCATCCAAGTGATGATCGCACCCAGTTTTGCTGA
TATTTTTTATGGCAACTCAATCAACAACCAAATGGTGCCCGTTCGCCTAACCGAATCTGAAGTGGATGAAATTTTTCAGT
TCGTTGAAGCGAATGAAGGTGCTGAGGTTACTGTGGATTTAGAAGCAATGTTAGTGACCGCTAACAATAAACAGTATTCT
TTTGAAATTGATGAATTTCGCCGCCATTGCCTGCTAAATGGTTTAGATAATATCGGGTTAACACTGCAACACGCCGATAA
AATTTCGGAATATGAAGCGAAGATCCCGAGTTTCTTAAAATAA

Upstream 100 bases:

>100_bases
AACTCACCTAGTGAGCCCTGCGATGGCTGCCGCTGCTGCAATTGCAGGTCACTTTGTTGATATCCGCACCATTACAGAAC
AAGCATAAAAGGAATTTACT

Downstream 100 bases:

>100_bases
GATTCAGGCTTCGTATTCAGGAAATGTTAGAGGTTGATATGAACGTATCAACCTCTTTTTTGTCTATAAAATAGTGTCAT
CTATAATCACATATAACTAA

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 200; Mature: 199

Protein sequence:

>200_residues
MSGFKQHTGLVVPLDTANIDTDAIIPKQFLQKVNRIGFGKHLFHDWRFLDDAGEQPNPEFVMNAPRYQGATVLLARENFG
CGSSREHAPWSLADYGIQVMIAPSFADIFYGNSINNQMVPVRLTESEVDEIFQFVEANEGAEVTVDLEAMLVTANNKQYS
FEIDEFRRHCLLNGLDNIGLTLQHADKISEYEAKIPSFLK

Sequences:

>Translated_200_residues
MSGFKQHTGLVVPLDTANIDTDAIIPKQFLQKVNRIGFGKHLFHDWRFLDDAGEQPNPEFVMNAPRYQGATVLLARENFG
CGSSREHAPWSLADYGIQVMIAPSFADIFYGNSINNQMVPVRLTESEVDEIFQFVEANEGAEVTVDLEAMLVTANNKQYS
FEIDEFRRHCLLNGLDNIGLTLQHADKISEYEAKIPSFLK
>Mature_199_residues
SGFKQHTGLVVPLDTANIDTDAIIPKQFLQKVNRIGFGKHLFHDWRFLDDAGEQPNPEFVMNAPRYQGATVLLARENFGC
GSSREHAPWSLADYGIQVMIAPSFADIFYGNSINNQMVPVRLTESEVDEIFQFVEANEGAEVTVDLEAMLVTANNKQYSF
EIDEFRRHCLLNGLDNIGLTLQHADKISEYEAKIPSFLK

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=196, Percent_Identity=70.9183673469388, Blast_Score=309, Evalue=8e-86,
Organism=Saccharomyces cerevisiae, GI6321429, Length=199, Percent_Identity=47.7386934673367, Blast_Score=189, Evalue=2e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 22541; Mature: 22409

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGFKQHTGLVVPLDTANIDTDAIIPKQFLQKVNRIGFGKHLFHDWRFLDDAGEQPNPEF
CCCCCHHCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCE
VMNAPRYQGATVLLARENFGCGSSREHAPWSLADYGIQVMIAPSFADIFYGNSINNQMVP
EEECCCCCCEEEEEEECCCCCCCCCCCCCEEHHCCCEEEEEECCHHHHHCCCCCCCCEEE
VRLTESEVDEIFQFVEANEGAEVTVDLEAMLVTANNKQYSFEIDEFRRHCLLNGLDNIGL
EEECHHHHHHHHHHHHCCCCCEEEEEEEEEEEEECCCEEEEEHHHHHHHHHHCCCCCCCE
TLQHADKISEYEAKIPSFLK
EEEHHHHHHHHHHCCCHHCC
>Mature Secondary Structure 
SGFKQHTGLVVPLDTANIDTDAIIPKQFLQKVNRIGFGKHLFHDWRFLDDAGEQPNPEF
CCCCHHCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCE
VMNAPRYQGATVLLARENFGCGSSREHAPWSLADYGIQVMIAPSFADIFYGNSINNQMVP
EEECCCCCCEEEEEEECCCCCCCCCCCCCEEHHCCCEEEEEECCHHHHHCCCCCCCCEEE
VRLTESEVDEIFQFVEANEGAEVTVDLEAMLVTANNKQYSFEIDEFRRHCLLNGLDNIGL
EEECHHHHHHHHHHHHCCCCCEEEEEEEEEEEEECCCEEEEEHHHHHHHHHHCCCCCCCE
TLQHADKISEYEAKIPSFLK
EEEHHHHHHHHHHCCCHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA