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The map label for this gene is greB [H]

Identifier: 59710728

GI number: 59710728

Start: 140927

End: 141400

Strand: Reverse

Name: greB [H]

Synonym: VF_0121

Alternate gene names: 59710728

Gene position: 141400-140927 (Counterclockwise)

Preceding gene: 59710729

Following gene: 59710727

Centisome position: 4.88

GC content: 42.83

Gene sequence:

>474_bases
ATGAAAACCAATTTAATCACACGTGAAGGCTTTAATAAGCTACAAGATGAACTCACGTTTCTATGGAAAGAAGAACGCCC
AGAAGTCACAAAAAAAGTGACTTGGGCGGCTAGCCTTGGTGATCGCAGTGAAAACGCTGATTATCAATACAACAAAAAAC
GCCTTCGTGAAATAGACCGCCGTGTTCGCTACCTTCGTAAACGTTTAGACCAAGTAAAAGTGGTCGACTATTCCCCACAG
CAAGACGGAAAAATCTTTTTCGGTGCGTTCGTTGAAATTGAAAACGAACAAGGCGATACCCTCTCTTTTCGCATCGTTGG
TCCTGATGAAATATTTGGCCGCAACGACTATATCTCTATCGATTCCCCAATGGCTCGTGCACTGATCAAAAAAGAAGTGG
ATGAAGATGTGATTGTAAAAACGCCTGATGGGGATAAAGAGTGGTTCGTGAATTCGATTAGGTACCAGGTGTAG

Upstream 100 bases:

>100_bases
TATTCCTCAATGGATGTGGCTACATAAACGTTTTAAAACAATGGAAGATCCAAACGTTAAAAAAGGCATCCGCTACGATT
AGGGCGTGTCAGGATAAATA

Downstream 100 bases:

>100_bases
GGACGAGATTCGAGGACGCTTCGCTCGAGGGACGAGGAAGAGCTGAGAGCAGAACTCAGATCGGTCGCAAGCTCCCTTGC
AGAGTTCAGAAGTGATCGTT

Product: transcription elongation factor GreB

Products: NA

Alternate protein names: Transcript cleavage factor greB [H]

Number of amino acids: Translated: 157; Mature: 157

Protein sequence:

>157_residues
MKTNLITREGFNKLQDELTFLWKEERPEVTKKVTWAASLGDRSENADYQYNKKRLREIDRRVRYLRKRLDQVKVVDYSPQ
QDGKIFFGAFVEIENEQGDTLSFRIVGPDEIFGRNDYISIDSPMARALIKKEVDEDVIVKTPDGDKEWFVNSIRYQV

Sequences:

>Translated_157_residues
MKTNLITREGFNKLQDELTFLWKEERPEVTKKVTWAASLGDRSENADYQYNKKRLREIDRRVRYLRKRLDQVKVVDYSPQ
QDGKIFFGAFVEIENEQGDTLSFRIVGPDEIFGRNDYISIDSPMARALIKKEVDEDVIVKTPDGDKEWFVNSIRYQV
>Mature_157_residues
MKTNLITREGFNKLQDELTFLWKEERPEVTKKVTWAASLGDRSENADYQYNKKRLREIDRRVRYLRKRLDQVKVVDYSPQ
QDGKIFFGAFVEIENEQGDTLSFRIVGPDEIFGRNDYISIDSPMARALIKKEVDEDVIVKTPDGDKEWFVNSIRYQV

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082261, Length=155, Percent_Identity=74.8387096774194, Blast_Score=243, Evalue=3e-66,
Organism=Escherichia coli, GI87082225, Length=157, Percent_Identity=35.031847133758, Blast_Score=96, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR001437
- InterPro:   IPR022691
- InterPro:   IPR006358 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 18557; Mature: 18557

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS00829 GREAB_1 ; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTNLITREGFNKLQDELTFLWKEERPEVTKKVTWAASLGDRSENADYQYNKKRLREIDR
CCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHEEEEHHCCCCCCCCCCHHHHHHHHHHHH
RVRYLRKRLDQVKVVDYSPQQDGKIFFGAFVEIENEQGDTLSFRIVGPDEIFGRNDYISI
HHHHHHHHHHHEEEEECCCCCCCCEEEEEEEEEECCCCCEEEEEEECCHHHCCCCCEEEC
DSPMARALIKKEVDEDVIVKTPDGDKEWFVNSIRYQV
CCHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHEECC
>Mature Secondary Structure
MKTNLITREGFNKLQDELTFLWKEERPEVTKKVTWAASLGDRSENADYQYNKKRLREIDR
CCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHEEEEHHCCCCCCCCCCHHHHHHHHHHHH
RVRYLRKRLDQVKVVDYSPQQDGKIFFGAFVEIENEQGDTLSFRIVGPDEIFGRNDYISI
HHHHHHHHHHHEEEEECCCCCCCCEEEEEEEEEECCCCCEEEEEEECCHHHCCCCCEEEC
DSPMARALIKKEVDEDVIVKTPDGDKEWFVNSIRYQV
CCHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]