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The map label for this gene is htrB2 [H]

Identifier: 59710729

GI number: 59710729

Start: 141419

End: 142357

Strand: Reverse

Name: htrB2 [H]

Synonym: VF_0122

Alternate gene names: 59710729

Gene position: 142357-141419 (Counterclockwise)

Preceding gene: 172087641

Following gene: 59710728

Centisome position: 4.91

GC content: 41.21

Gene sequence:

>939_bases
ATGTCAAAGTATCCAGCACCTAAATTCTCTAGCGCTCTTCTACACCCTAAATATTGGGGGGTATGGTTTGGATTCGGATT
CCTTTTTGCCATCGTCACCCTATTACCTTATAAAGTAACTTACAAACTTGGTCGCTCTCTTGGTTTACTTGGATTAAAAC
TCGGAAAATCAAGAGCCCATGTTGCTCGCCGTAATTTAGAACTCGCTTTTCCAGAAATGGAATCGTCTGAGCGTGAAGCC
ATTATTGTTGAGAATTTCAAAAATTCAGGTCTTGCGATTTTTGAAACCGCAATGGCTTGGGTATGGCCAGATTGGCGTGT
CGAAAAACATTTTAGTTATGAAAACAAGCAGCACATTCTTGATCTTGCAGAAGAAGGTCGTGGCGTATTAGTTGTTTGTG
TTCACGCATTAAACCTTGAACTTACCGCTCGTGCATTCACTTCTTTTGCTCCAGGTTATGGTGTTTACCGCCCACATAAC
AACCCAGCTTACGATTTCATTCAACATTGGGGCCGAACCAATAATGGCAACCAAATGATTGATAGAAAAGACGTCAAAGG
CATGCTGCGTGTATTACGTAAAGGTGAGCGTCTTTGGTATATGCCTGACCACGATTACAACCGCAATAAATCGGTCTTTG
TTCCTTTCTTTGCGGTTCCAGATGCTTGTACTACCGTAGGCTCGGATATATTAATCAGTGCAAGTAAATGTGCCGTTGTT
ACTGCATCGGGCTTTAGAACTTACAATAATTACCACTTACAAATTGATAATGACATCAGTGACCAATTTCCGAAGAAAGA
TCCCGTTGGTGTTGCTACGGTAATGAATAAAGCGGTAGAACGTGTCATTTTACGCTGTATTCCTCAATGGATGTGGCTAC
ATAAACGTTTTAAAACAATGGAAGATCCAAACGTTAAAAAAGGCATCCGCTACGATTAG

Upstream 100 bases:

>100_bases
TCGTTCTGATTTTAAATATCAACCGACTGAAAACTTTAACGAGTATTGGGCGCTACTCAGTGCTCAGATCCAATAATTAA
TTAGATAAAATAGGCTCACG

Downstream 100 bases:

>100_bases
GGCGTGTCAGGATAAATAATGAAAACCAATTTAATCACACGTGAAGGCTTTAATAAGCTACAAGATGAACTCACGTTTCT
ATGGAAAGAAGAACGCCCAG

Product: lipid A biosynthesis lauroyl acyltransferase

Products: KDO2-(lauroyl)-lipid IVA [C]

Alternate protein names: Heat shock protein B [H]

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MSKYPAPKFSSALLHPKYWGVWFGFGFLFAIVTLLPYKVTYKLGRSLGLLGLKLGKSRAHVARRNLELAFPEMESSEREA
IIVENFKNSGLAIFETAMAWVWPDWRVEKHFSYENKQHILDLAEEGRGVLVVCVHALNLELTARAFTSFAPGYGVYRPHN
NPAYDFIQHWGRTNNGNQMIDRKDVKGMLRVLRKGERLWYMPDHDYNRNKSVFVPFFAVPDACTTVGSDILISASKCAVV
TASGFRTYNNYHLQIDNDISDQFPKKDPVGVATVMNKAVERVILRCIPQWMWLHKRFKTMEDPNVKKGIRYD

Sequences:

>Translated_312_residues
MSKYPAPKFSSALLHPKYWGVWFGFGFLFAIVTLLPYKVTYKLGRSLGLLGLKLGKSRAHVARRNLELAFPEMESSEREA
IIVENFKNSGLAIFETAMAWVWPDWRVEKHFSYENKQHILDLAEEGRGVLVVCVHALNLELTARAFTSFAPGYGVYRPHN
NPAYDFIQHWGRTNNGNQMIDRKDVKGMLRVLRKGERLWYMPDHDYNRNKSVFVPFFAVPDACTTVGSDILISASKCAVV
TASGFRTYNNYHLQIDNDISDQFPKKDPVGVATVMNKAVERVILRCIPQWMWLHKRFKTMEDPNVKKGIRYD
>Mature_311_residues
SKYPAPKFSSALLHPKYWGVWFGFGFLFAIVTLLPYKVTYKLGRSLGLLGLKLGKSRAHVARRNLELAFPEMESSEREAI
IVENFKNSGLAIFETAMAWVWPDWRVEKHFSYENKQHILDLAEEGRGVLVVCVHALNLELTARAFTSFAPGYGVYRPHNN
PAYDFIQHWGRTNNGNQMIDRKDVKGMLRVLRKGERLWYMPDHDYNRNKSVFVPFFAVPDACTTVGSDILISASKCAVVT
ASGFRTYNNYHLQIDNDISDQFPKKDPVGVATVMNKAVERVILRCIPQWMWLHKRFKTMEDPNVKKGIRYD

Specific function: Acylates the intermediate (KDO)2-lipid IVA to form (KDO)2-(lauroyl)-lipid IVA. Has 10 fold selectivity for lauroyl- ACP over myristoyl-ACP [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1787292, Length=294, Percent_Identity=46.5986394557823, Blast_Score=273, Evalue=8e-75,
Organism=Escherichia coli, GI87082094, Length=298, Percent_Identity=41.6107382550336, Blast_Score=238, Evalue=3e-64,
Organism=Escherichia coli, GI1788161, Length=309, Percent_Identity=26.2135922330097, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011920 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.- [C]

Molecular weight: Translated: 35898; Mature: 35767

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKYPAPKFSSALLHPKYWGVWFGFGFLFAIVTLLPYKVTYKLGRSLGLLGLKLGKSRAH
CCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHH
VARRNLELAFPEMESSEREAIIVENFKNSGLAIFETAMAWVWPDWRVEKHFSYENKQHIL
HHHHCCEEECCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCEECCCCCCCCHHHHH
DLAEEGRGVLVVCVHALNLELTARAFTSFAPGYGVYRPHNNPAYDFIQHWGRTNNGNQMI
HHHHCCCCEEEEEEEHHCCEEHHHHHHHCCCCCCEECCCCCCHHHHHHHCCCCCCCCCCC
DRKDVKGMLRVLRKGERLWYMPDHDYNRNKSVFVPFFAVPDACTTVGSDILISASKCAVV
CHHHHHHHHHHHHCCCEEEECCCCCCCCCCCEEEEEEECCHHHHHCCCCCEEECCCEEEE
TASGFRTYNNYHLQIDNDISDQFPKKDPVGVATVMNKAVERVILRCIPQWMWLHKRFKTM
EECCCEEECCEEEEECCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EDPNVKKGIRYD
CCCCCCCCCCCC
>Mature Secondary Structure 
SKYPAPKFSSALLHPKYWGVWFGFGFLFAIVTLLPYKVTYKLGRSLGLLGLKLGKSRAH
CCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHH
VARRNLELAFPEMESSEREAIIVENFKNSGLAIFETAMAWVWPDWRVEKHFSYENKQHIL
HHHHCCEEECCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCEECCCCCCCCHHHHH
DLAEEGRGVLVVCVHALNLELTARAFTSFAPGYGVYRPHNNPAYDFIQHWGRTNNGNQMI
HHHHCCCCEEEEEEEHHCCEEHHHHHHHCCCCCCEECCCCCCHHHHHHHCCCCCCCCCCC
DRKDVKGMLRVLRKGERLWYMPDHDYNRNKSVFVPFFAVPDACTTVGSDILISASKCAVV
CHHHHHHHHHHHHCCCEEEECCCCCCCCCCCEEEEEEECCHHHHHCCCCCEEECCCEEEE
TASGFRTYNNYHLQIDNDISDQFPKKDPVGVATVMNKAVERVILRCIPQWMWLHKRFKTM
EECCCEEECCEEEEECCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EDPNVKKGIRYD
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: KDO2-lipid IVA; lauroyl-ACP [C]

Specific reaction: KDO2-lipid IVA + lauroyl-ACP = KDO2-(lauroyl)-lipid IVA + [acyl carrier protein] [C]

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]