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The map label for this gene is gmk

Identifier: 59710713

GI number: 59710713

Start: 124997

End: 125620

Strand: Reverse

Name: gmk

Synonym: VF_0106

Alternate gene names: 59710713

Gene position: 125620-124997 (Counterclockwise)

Preceding gene: 59710717

Following gene: 59710712

Centisome position: 4.34

GC content: 40.22

Gene sequence:

>624_bases
ATGAGCAAAGGTACACTTTATATTGTGTCAGCCCCAAGTGGTGCTGGTAAGTCGAGTTTGATCTCGGCACTGTTAGAAAG
CAACCCAACTTATGCTATGAAAGTCTCTGTGTCTCATACTACTCGTGGTATGCGCCCAGGAGAAACTGATGGTGTTCATT
ATCACTTTATTCAGAAAGAGCATTTTGAAGAACTGATTCAAAAAGGTGAATTCTTAGAATATGCCGAAGTGTTCGGTAAC
TACTATGGAACATCTCGAGTATGGATCGAAGAGACCCTTGATAAAGGTATTGATGTGTTCTTAGATATTGATTGGCAAGG
TGCTCGTCAAATCCGTGAACAAATGCCTTTAGCAAAAAGCGTATTCATTCTACCTCCATCAAATGGAGAACTAGAACGAC
GCCTAAATGCACGTGGCCAAGACAGCGATGCTGTTATTGCTAAGCGCATGAGCGAAGCAAAATCTGAAATTTCACATTAC
GATGAATACGATTATGTGATCATTAATGACGATTTTGATACTGCACAAATGGACTTCCGTTCAATTATCCGTGCAGAACG
CTTAAAACAAGACAAACAAACAACGAAATATAAAGGCATGTTAGAAGCGCTATTAGCGGACTAA

Upstream 100 bases:

>100_bases
CTATTCTTCGCCACCCAATTTTCTATGCTTCGCATTTCCTCTCAATATCTAAAAAAATGGTATATTAAGAGAAATGCCAT
AACTCCCAAGAGAATCTATC

Downstream 100 bases:

>100_bases
TTTCTTCATATTTCTACGAGTATCTTGTAACCCTAGCTAGAAACTTATACGGGTTACTTGTATACTTTCTCATCATATAA
CTATTTTTGTTAATAAATTG

Product: guanylate kinase

Products: NA

Alternate protein names: GMP kinase

Number of amino acids: Translated: 207; Mature: 206

Protein sequence:

>207_residues
MSKGTLYIVSAPSGAGKSSLISALLESNPTYAMKVSVSHTTRGMRPGETDGVHYHFIQKEHFEELIQKGEFLEYAEVFGN
YYGTSRVWIEETLDKGIDVFLDIDWQGARQIREQMPLAKSVFILPPSNGELERRLNARGQDSDAVIAKRMSEAKSEISHY
DEYDYVIINDDFDTAQMDFRSIIRAERLKQDKQTTKYKGMLEALLAD

Sequences:

>Translated_207_residues
MSKGTLYIVSAPSGAGKSSLISALLESNPTYAMKVSVSHTTRGMRPGETDGVHYHFIQKEHFEELIQKGEFLEYAEVFGN
YYGTSRVWIEETLDKGIDVFLDIDWQGARQIREQMPLAKSVFILPPSNGELERRLNARGQDSDAVIAKRMSEAKSEISHY
DEYDYVIINDDFDTAQMDFRSIIRAERLKQDKQTTKYKGMLEALLAD
>Mature_206_residues
SKGTLYIVSAPSGAGKSSLISALLESNPTYAMKVSVSHTTRGMRPGETDGVHYHFIQKEHFEELIQKGEFLEYAEVFGNY
YGTSRVWIEETLDKGIDVFLDIDWQGARQIREQMPLAKSVFILPPSNGELERRLNARGQDSDAVIAKRMSEAKSEISHYD
EYDYVIINDDFDTAQMDFRSIIRAERLKQDKQTTKYKGMLEALLAD

Specific function: Essential for recycling GMP and indirectly, cGMP

COG id: COG0194

COG function: function code F; Guanylate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 guanylate kinase-like domain

Homologues:

Organism=Homo sapiens, GI226874831, Length=197, Percent_Identity=36.0406091370558, Blast_Score=123, Evalue=9e-29,
Organism=Homo sapiens, GI226874833, Length=197, Percent_Identity=36.0406091370558, Blast_Score=123, Evalue=9e-29,
Organism=Homo sapiens, GI4504221, Length=197, Percent_Identity=36.0406091370558, Blast_Score=123, Evalue=9e-29,
Organism=Homo sapiens, GI14780902, Length=193, Percent_Identity=31.0880829015544, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI111154074, Length=156, Percent_Identity=35.8974358974359, Blast_Score=77, Evalue=9e-15,
Organism=Homo sapiens, GI38570142, Length=180, Percent_Identity=28.3333333333333, Blast_Score=75, Evalue=3e-14,
Organism=Homo sapiens, GI4505237, Length=171, Percent_Identity=31.5789473684211, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI262118259, Length=171, Percent_Identity=31.5789473684211, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI262118255, Length=171, Percent_Identity=31.5789473684211, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI262118263, Length=171, Percent_Identity=31.5789473684211, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI21361598, Length=204, Percent_Identity=30.3921568627451, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI21536464, Length=195, Percent_Identity=25.6410256410256, Blast_Score=67, Evalue=9e-12,
Organism=Homo sapiens, GI21389483, Length=184, Percent_Identity=27.7173913043478, Blast_Score=65, Evalue=3e-11,
Organism=Escherichia coli, GI1790080, Length=207, Percent_Identity=63.768115942029, Blast_Score=285, Evalue=2e-78,
Organism=Caenorhabditis elegans, GI193203132, Length=190, Percent_Identity=37.3684210526316, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17508835, Length=190, Percent_Identity=37.3684210526316, Blast_Score=131, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17557440, Length=179, Percent_Identity=26.8156424581006, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71988041, Length=192, Percent_Identity=29.6875, Blast_Score=65, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71988048, Length=192, Percent_Identity=29.6875, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320662, Length=183, Percent_Identity=37.7049180327869, Blast_Score=133, Evalue=2e-32,
Organism=Drosophila melanogaster, GI21356141, Length=189, Percent_Identity=37.5661375661376, Blast_Score=125, Evalue=1e-29,
Organism=Drosophila melanogaster, GI85816281, Length=216, Percent_Identity=26.3888888888889, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI85816106, Length=216, Percent_Identity=26.3888888888889, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI116007136, Length=174, Percent_Identity=28.735632183908, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI45554489, Length=174, Percent_Identity=28.735632183908, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI45551002, Length=216, Percent_Identity=26.3888888888889, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI85724806, Length=174, Percent_Identity=28.735632183908, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24640542, Length=174, Percent_Identity=28.735632183908, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI45554481, Length=174, Percent_Identity=28.735632183908, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI161077626, Length=174, Percent_Identity=28.735632183908, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KGUA_VIBF1 (Q5E8P5)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_203489.1
- HSSP:   P60546
- ProteinModelPortal:   Q5E8P5
- SMR:   Q5E8P5
- STRING:   Q5E8P5
- GeneID:   3277846
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_0106
- NMPDR:   fig|312309.3.peg.138
- eggNOG:   COG0194
- HOGENOM:   HBG671982
- OMA:   DIDWQGT
- PhylomeDB:   Q5E8P5
- ProtClustDB:   PRK00300
- BioCyc:   VFIS312309:VF0106-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00328
- InterPro:   IPR008144
- InterPro:   IPR008145
- InterPro:   IPR020590
- InterPro:   IPR017665
- SMART:   SM00072
- TIGRFAMs:   TIGR03263

Pfam domain/function: PF00625 Guanylate_kin

EC number: =2.7.4.8

Molecular weight: Translated: 23557; Mature: 23426

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00856 GUANYLATE_KINASE_1; PS50052 GUANYLATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKGTLYIVSAPSGAGKSSLISALLESNPTYAMKVSVSHTTRGMRPGETDGVHYHFIQKE
CCCCEEEEEECCCCCCHHHHHHHHHHCCCCEEEEEEECHHHCCCCCCCCCCEEEEEECHH
HFEELIQKGEFLEYAEVFGNYYGTSRVWIEETLDKGIDVFLDIDWQGARQIREQMPLAKS
HHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEEEECCHHHHHHHHHCCCHHE
VFILPPSNGELERRLNARGQDSDAVIAKRMSEAKSEISHYDEYDYVIINDDFDTAQMDFR
EEEECCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHH
SIIRAERLKQDKQTTKYKGMLEALLAD
HHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKGTLYIVSAPSGAGKSSLISALLESNPTYAMKVSVSHTTRGMRPGETDGVHYHFIQKE
CCCEEEEEECCCCCCHHHHHHHHHHCCCCEEEEEEECHHHCCCCCCCCCCEEEEEECHH
HFEELIQKGEFLEYAEVFGNYYGTSRVWIEETLDKGIDVFLDIDWQGARQIREQMPLAKS
HHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEEEECCHHHHHHHHHCCCHHE
VFILPPSNGELERRLNARGQDSDAVIAKRMSEAKSEISHYDEYDYVIINDDFDTAQMDFR
EEEECCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHH
SIIRAERLKQDKQTTKYKGMLEALLAD
HHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA