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The map label for this gene is pcnB [H]

Identifier: 172087758

GI number: 172087758

Start: 2436589

End: 2438016

Strand: Direct

Name: pcnB [H]

Synonym: VF_2166

Alternate gene names: 172087758

Gene position: 2436589-2438016 (Clockwise)

Preceding gene: 172087757

Following gene: 59712775

Centisome position: 84.09

GC content: 40.34

Gene sequence:

>1428_bases
TTGACTGCTCACTTGAATATCGAGGTTATTATCTTTAATCAAGTTGCCCGTTTTTGTCGTAATTTACTCAAAAACAACAG
TGATATTTGCTCAACAGAGGATTTGAAACTGAACGTTATCACACGCCAAGAACATAATATTTCACGTAAAGATTTAAGTG
ATAATGCACTTAAAGTTCTTTACCGTCTAAGCAATGCTGGATATGACGCTTATCTGGTAGGCGGAGGTGTGCGCGATATT
CTATTAGGTCAAGAACCTAAAGACTTTGATATTGCAACCAATGCAACTCCAGAGCAAATCAAAAAACTATTCAGAAATTG
TCGTTTAATTGGTCGACGATTCCGTCTTGCTCATATTCTTTTTGGTCGAGATGTTATTGAAGTTGCTACCTTCCGTGGCC
ACCACAAAGAAGATAATGAGAAAAAGAATATCTCAGCGCAATCTGACGAAGGGATGCTACTACGCGATAACGTATATGGC
ACGGTAGAAGAAGATGCTGAACGTCGTGACTTTACGGTTAATGCGATGTACTACAATATCGCAGACTACTCGATACACGA
TTACGCCAACGGCATTGAAGATTTAAAAAATGGCATTATCCGTTTAATTGGTGATCCTGAAACTCGTTATCGCGAAGACC
CAGTCCGTATGCTTCGTGCAGTTCGTTTTGCTGCAAAATTAGATATGACTATTTGTGATAAAACAGCAGCACCAATTGCT
GAGCTTTCACCTCTACTTAGAGACATTCCTGCTGCACGTTTATTTGAAGAGTCATTAAAGCTACTGCAAACCGGTAATGG
ATTAAATACTTACTGGATGCTACGAGACTTTAATCTTTTCCAACAATTGTTCCCTGTACTCACTCCTTTCTTAACGGAAG
AAGGAAATAGCCCTGCTGAACAAATGATTGAGCTTGTATTACGCTCTACAGATAAGCGAATTAACAGCGGTAAGCGTGTA
AACCCTGCGTTTATGTTTGCTGCAATGCTTTGGTATCCGTTAACGGCAAAAGCGCAAGAAATCCAAGATGACAATCCAAA
ATTTGATCACTACGAATCAATAATGGAAGCATCTAATTTAATTCTTGATACTCAAGTAAGAAGTACTGCGATCCCACGTC
GCTTAACTGCAACAATTCGTGATATTTGGCAACTTCAATTACGCTTACCTCGTCGCACGGGTAAACGAGCTTTTGTACTT
ATTGAGCAACAAAAATTCCGTGCCGCTTATGATTTCTTAGAAATGCGTGGCAAAGTTGAAGGCGGCGAAACCAAAGATTT
ATGTGACTGGTGGACTGAGTTCCAAGATGCACCAGCACCACATCGTAACAATATGGTTCAAAATTTAAACGGTGGTCGCC
GTTCTGGTGGTCCTCGTAAGCGTCGCAGCCCATATCGTAAGAAAAAAAGAGCAAATAACGACTCATGA

Upstream 100 bases:

>100_bases
GGGCAATCCAACATTGGCAATTAAATCAGCTTCCAACTGGGCTCGAGATCACTACCCCATTCTCAAAACCCTCAATTTAA
GCTATGATGTGCGCCTATTT

Downstream 100 bases:

>100_bases
TCAAAGTAATTATTGCGATTGGGAGTAACCTTGGAGATCCCGTAGCTCAAGCAAAAGTGGCTATCAATGCCCTGCACCAG
CTCCCGGACTCTAAAGTAAC

Product: poly(A) polymerase I

Products: NA

Alternate protein names: PAP; Plasmid copy number protein [H]

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MTAHLNIEVIIFNQVARFCRNLLKNNSDICSTEDLKLNVITRQEHNISRKDLSDNALKVLYRLSNAGYDAYLVGGGVRDI
LLGQEPKDFDIATNATPEQIKKLFRNCRLIGRRFRLAHILFGRDVIEVATFRGHHKEDNEKKNISAQSDEGMLLRDNVYG
TVEEDAERRDFTVNAMYYNIADYSIHDYANGIEDLKNGIIRLIGDPETRYREDPVRMLRAVRFAAKLDMTICDKTAAPIA
ELSPLLRDIPAARLFEESLKLLQTGNGLNTYWMLRDFNLFQQLFPVLTPFLTEEGNSPAEQMIELVLRSTDKRINSGKRV
NPAFMFAAMLWYPLTAKAQEIQDDNPKFDHYESIMEASNLILDTQVRSTAIPRRLTATIRDIWQLQLRLPRRTGKRAFVL
IEQQKFRAAYDFLEMRGKVEGGETKDLCDWWTEFQDAPAPHRNNMVQNLNGGRRSGGPRKRRSPYRKKKRANNDS

Sequences:

>Translated_475_residues
MTAHLNIEVIIFNQVARFCRNLLKNNSDICSTEDLKLNVITRQEHNISRKDLSDNALKVLYRLSNAGYDAYLVGGGVRDI
LLGQEPKDFDIATNATPEQIKKLFRNCRLIGRRFRLAHILFGRDVIEVATFRGHHKEDNEKKNISAQSDEGMLLRDNVYG
TVEEDAERRDFTVNAMYYNIADYSIHDYANGIEDLKNGIIRLIGDPETRYREDPVRMLRAVRFAAKLDMTICDKTAAPIA
ELSPLLRDIPAARLFEESLKLLQTGNGLNTYWMLRDFNLFQQLFPVLTPFLTEEGNSPAEQMIELVLRSTDKRINSGKRV
NPAFMFAAMLWYPLTAKAQEIQDDNPKFDHYESIMEASNLILDTQVRSTAIPRRLTATIRDIWQLQLRLPRRTGKRAFVL
IEQQKFRAAYDFLEMRGKVEGGETKDLCDWWTEFQDAPAPHRNNMVQNLNGGRRSGGPRKRRSPYRKKKRANNDS
>Mature_474_residues
TAHLNIEVIIFNQVARFCRNLLKNNSDICSTEDLKLNVITRQEHNISRKDLSDNALKVLYRLSNAGYDAYLVGGGVRDIL
LGQEPKDFDIATNATPEQIKKLFRNCRLIGRRFRLAHILFGRDVIEVATFRGHHKEDNEKKNISAQSDEGMLLRDNVYGT
VEEDAERRDFTVNAMYYNIADYSIHDYANGIEDLKNGIIRLIGDPETRYREDPVRMLRAVRFAAKLDMTICDKTAAPIAE
LSPLLRDIPAARLFEESLKLLQTGNGLNTYWMLRDFNLFQQLFPVLTPFLTEEGNSPAEQMIELVLRSTDKRINSGKRVN
PAFMFAAMLWYPLTAKAQEIQDDNPKFDHYESIMEASNLILDTQVRSTAIPRRLTATIRDIWQLQLRLPRRTGKRAFVLI
EQQKFRAAYDFLEMRGKVEGGETKDLCDWWTEFQDAPAPHRNNMVQNLNGGRRSGGPRKRRSPYRKKKRANNDS

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's. Seems to be involved in plasmid copy number control [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=329, Percent_Identity=28.5714285714286, Blast_Score=100, Evalue=4e-21,
Organism=Escherichia coli, GI87081691, Length=443, Percent_Identity=57.3363431151242, Blast_Score=537, Evalue=1e-154,
Organism=Escherichia coli, GI1789436, Length=221, Percent_Identity=33.0316742081448, Blast_Score=80, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71995920, Length=214, Percent_Identity=28.0373831775701, Blast_Score=77, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6321016, Length=194, Percent_Identity=30.4123711340206, Blast_Score=80, Evalue=6e-16,
Organism=Drosophila melanogaster, GI281360112, Length=235, Percent_Identity=29.7872340425532, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21357337, Length=235, Percent_Identity=29.7872340425532, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 54791; Mature: 54660

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAHLNIEVIIFNQVARFCRNLLKNNSDICSTEDLKLNVITRQEHNISRKDLSDNALKVL
CCEEEEEEEEEHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCHHCCCHHHHHHH
YRLSNAGYDAYLVGGGVRDILLGQEPKDFDIATNATPEQIKKLFRNCRLIGRRFRLAHIL
HHHHCCCCEEEEECCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FGRDVIEVATFRGHHKEDNEKKNISAQSDEGMLLRDNVYGTVEEDAERRDFTVNAMYYNI
HCCHHHHHHHHCCCCCCCCCHHCCCCCCCCCEEEECCCCCCCHHCCCCCCEEEEEEEEEE
ADYSIHDYANGIEDLKNGIIRLIGDPETRYREDPVRMLRAVRFAAKLDMTICDKTAAPIA
ECCCHHHHHHHHHHHHCCCEEEECCCHHHHCCCHHHHHHHHHHHHHCCHHHHCCCCCCHH
ELSPLLRDIPAARLFEESLKLLQTGNGLNTYWMLRDFNLFQQLFPVLTPFLTEEGNSPAE
HHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCHHH
QMIELVLRSTDKRINSGKRVNPAFMFAAMLWYPLTAKAQEIQDDNPKFDHYESIMEASNL
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHCCCCCCHHHHHHHHHHHCC
ILDTQVRSTAIPRRLTATIRDIWQLQLRLPRRTGKRAFVLIEQQKFRAAYDFLEMRGKVE
EEECHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHHHHHCCCCC
GGETKDLCDWWTEFQDAPAPHRNNMVQNLNGGRRSGGPRKRRSPYRKKKRANNDS
CCCCHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCCHHCCHHHHHHCCCCCC
>Mature Secondary Structure 
TAHLNIEVIIFNQVARFCRNLLKNNSDICSTEDLKLNVITRQEHNISRKDLSDNALKVL
CEEEEEEEEEHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCHHCCCHHHHHHH
YRLSNAGYDAYLVGGGVRDILLGQEPKDFDIATNATPEQIKKLFRNCRLIGRRFRLAHIL
HHHHCCCCEEEEECCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FGRDVIEVATFRGHHKEDNEKKNISAQSDEGMLLRDNVYGTVEEDAERRDFTVNAMYYNI
HCCHHHHHHHHCCCCCCCCCHHCCCCCCCCCEEEECCCCCCCHHCCCCCCEEEEEEEEEE
ADYSIHDYANGIEDLKNGIIRLIGDPETRYREDPVRMLRAVRFAAKLDMTICDKTAAPIA
ECCCHHHHHHHHHHHHCCCEEEECCCHHHHCCCHHHHHHHHHHHHHCCHHHHCCCCCCHH
ELSPLLRDIPAARLFEESLKLLQTGNGLNTYWMLRDFNLFQQLFPVLTPFLTEEGNSPAE
HHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCHHH
QMIELVLRSTDKRINSGKRVNPAFMFAAMLWYPLTAKAQEIQDDNPKFDHYESIMEASNL
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHCCCCCCHHHHHHHHHHHCC
ILDTQVRSTAIPRRLTATIRDIWQLQLRLPRRTGKRAFVLIEQQKFRAAYDFLEMRGKVE
EEECHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHHHHHCCCCC
GGETKDLCDWWTEFQDAPAPHRNNMVQNLNGGRRSGGPRKRRSPYRKKKRANNDS
CCCCHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCCHHCCHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]