Click here to switch to the map view.

The map label for this gene is folK [H]

Identifier: 59712775

GI number: 59712775

Start: 2438013

End: 2438498

Strand: Direct

Name: folK [H]

Synonym: VF_2168

Alternate gene names: 59712775

Gene position: 2438013-2438498 (Clockwise)

Preceding gene: 172087758

Following gene: 59712776

Centisome position: 84.14

GC content: 41.98

Gene sequence:

>486_bases
ATGATCAAAGTAATTATTGCGATTGGGAGTAACCTTGGAGATCCCGTAGCTCAAGCAAAAGTGGCTATCAATGCCCTGCA
CCAGCTCCCGGACTCTAAAGTAACAGCGGTATCATCGCTTTACAGCAGTTCACCTATGGGTCCTCAAGATCAACCTGACT
ACATTAATGCAGTTGTTGAGATTGAAACCAACCTTGCTCCAATGGAATTACTGGATCTCACTCAAAAGATTGAGCTTGAG
CAAGGTCGCGTGCGTAAGGAAGAGCGCTGGGGACCAAGAACTTTAGATCTCGATATTATTTTATTTGGCGATCAAACGAT
CGACAATGAGCGATTAACCGTGCCTCACTATGGCATGAAGGAACGTGAATTTGTTCTTTATCCGCTCGCTGAAATTTATA
ATGATTTAACTTTACCTGATGGCACAACCCTTTCTCAGTTACTAAAAACTGTTGATAGAAATGGTTTGTCTATTTGGGAA
GATTAA

Upstream 100 bases:

>100_bases
GTAACAATATGGTTCAAAATTTAAACGGTGGTCGCCGTTCTGGTGGTCCTCGTAAGCGTCGCAGCCCATATCGTAAGAAA
AAAAGAGCAAATAACGACTC

Downstream 100 bases:

>100_bases
TCGCTTCTAGTAAGGATGTTTTATGAAAAAAATCTCTATTCATGACTTAATGAAGTGGAAGCAAGAAGGCAAAAAATTTG
CCACTGTTACAGCATATGAT

Product: 2-amino-4-hydroxy-6-hydroxymethyldihyropteridine pyrophosphokinase

Products: NA

Alternate protein names: 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase; PPPK; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase; HPPK [H]

Number of amino acids: Translated: 161; Mature: 161

Protein sequence:

>161_residues
MIKVIIAIGSNLGDPVAQAKVAINALHQLPDSKVTAVSSLYSSSPMGPQDQPDYINAVVEIETNLAPMELLDLTQKIELE
QGRVRKEERWGPRTLDLDIILFGDQTIDNERLTVPHYGMKEREFVLYPLAEIYNDLTLPDGTTLSQLLKTVDRNGLSIWE
D

Sequences:

>Translated_161_residues
MIKVIIAIGSNLGDPVAQAKVAINALHQLPDSKVTAVSSLYSSSPMGPQDQPDYINAVVEIETNLAPMELLDLTQKIELE
QGRVRKEERWGPRTLDLDIILFGDQTIDNERLTVPHYGMKEREFVLYPLAEIYNDLTLPDGTTLSQLLKTVDRNGLSIWE
D
>Mature_161_residues
MIKVIIAIGSNLGDPVAQAKVAINALHQLPDSKVTAVSSLYSSSPMGPQDQPDYINAVVEIETNLAPMELLDLTQKIELE
QGRVRKEERWGPRTLDLDIILFGDQTIDNERLTVPHYGMKEREFVLYPLAEIYNDLTLPDGTTLSQLLKTVDRNGLSIWE
D

Specific function: Dihydrofolate biosynthesis; first step. [C]

COG id: COG0801

COG function: function code H; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HPPK family [H]

Homologues:

Organism=Escherichia coli, GI1786335, Length=159, Percent_Identity=58.4905660377358, Blast_Score=195, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6324073, Length=141, Percent_Identity=41.1347517730496, Blast_Score=101, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000550 [H]

Pfam domain/function: PF01288 HPPK [H]

EC number: =2.7.6.3 [H]

Molecular weight: Translated: 18017; Mature: 18017

Theoretical pI: Translated: 4.26; Mature: 4.26

Prosite motif: PS00794 HPPK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKVIIAIGSNLGDPVAQAKVAINALHQLPDSKVTAVSSLYSSSPMGPQDQPDYINAVVE
CEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEE
IETNLAPMELLDLTQKIELEQGRVRKEERWGPRTLDLDIILFGDQTIDNERLTVPHYGMK
EECCCCHHHHHHHHHHHHHHHCCCCHHHCCCCEEEEEEEEEECCCCCCCCEEECCCCCCC
EREFVLYPLAEIYNDLTLPDGTTLSQLLKTVDRNGLSIWED
CCCEEEEEHHHHHCCCCCCCCCCHHHHHHHHCCCCCEECCC
>Mature Secondary Structure
MIKVIIAIGSNLGDPVAQAKVAINALHQLPDSKVTAVSSLYSSSPMGPQDQPDYINAVVE
CEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEE
IETNLAPMELLDLTQKIELEQGRVRKEERWGPRTLDLDIILFGDQTIDNERLTVPHYGMK
EECCCCHHHHHHHHHHHHHHHCCCCHHHCCCCEEEEEEEEEECCCCCCCCEEECCCCCCC
EREFVLYPLAEIYNDLTLPDGTTLSQLLKTVDRNGLSIWED
CCCEEEEEHHHHHCCCCCCCCCCHHHHHHHHCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1325970; 8202364; 9278503; 1657875; 2537812; 10378268; 10452528 [H]