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The map label for this gene is yadB [H]

Identifier: 172087757

GI number: 172087757

Start: 2435675

End: 2436568

Strand: Direct

Name: yadB [H]

Synonym: VF_2653

Alternate gene names: 172087757

Gene position: 2435675-2436568 (Clockwise)

Preceding gene: 59712772

Following gene: 172087758

Centisome position: 84.06

GC content: 38.93

Gene sequence:

>894_bases
ATGACAACTCAATACATTGGTCGTTTTGCACCATCACCTTCTGGTCCATTACACTTTGGCTCTCTTGTTGCTGCGCTAGG
GAGCTACTTACAAGCTAAATCTCATCAAGGAAAATGGCTCCTTCGTATTGAAGATCTTGACCCACCAAGGGAAGTATCAG
GCGCCGCTTCCTTAATCATTCAAACCATAGAGAATTACGGTCTAGAATGGGACGAAGAAATTGTATACCAAAGTCAACGA
CACGATTTATATCAATCTCAAATAGATAAATGGTTGCAAGAAAACAACGCTTATTATTGCCAGTGCACACGTAAACAAAT
AAAAGAAGACGGTGGTTTTTATTTAGGTCACTGCAAAGAAAAGCACCATTCAGAAAAAAATTCTGCTGTTCGTTTGGTTA
TGCAACATCCTGTTGAATCATTTAATGATATTAAACATGGTACGATTACAATCCCAAGTAAACTTGCACACGAAGACTTT
ATTATTAAACGTCGAGATGGACTATTTGCTTATAATCTCGCCGTGGTTCTTGATGATATCGACCAAGGAGTTACCGAAGT
GGTTCGCGGTGCCGATCTCATTGAGCCAACAGGACGCCAAATATCACTTTACCGTCAATTGGGTGCGAATGAAGTTAACT
ACCTTCATTTACCCCTTGCTGTTGATGAAAAAGGCAATAAACTTTCTAAACAAAACTATGCACCCGCTATTGATAATAGT
AACCCTAAACCAACACTATTAGCTGCATTAACGTTTTTAGGGTTTATAATTCCATTAGAAGTAAAGCAAGCTACATTGAA
TGAAATTCTTCAATGGGCAATCCAACATTGGCAATTAAATCAGCTTCCAACTGGGCTCGAGATCACTACCCCATTCTCAA
AACCCTCAATTTAA

Upstream 100 bases:

>100_bases
CTTGCAGAAATCAAAGAAAAGCAAATGGCAGGTTAATACCTATCAAATCATAAAAAAGGGAGCATTCGCTCCCTTTTTTA
CGTTTAAATAGTGAATATTT

Downstream 100 bases:

>100_bases
GCTATGATGTGCGCCTATTTTTGACTGCTCACTTGAATATCGAGGTTATTATCTTTAATCAAGTTGCCCGTTTTTGTCGT
AATTTACTCAAAAACAACAG

Product: glutamyl-Q tRNA(Asp) synthetase

Products: NA

Alternate protein names: Glu-Q-RSs [H]

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MTTQYIGRFAPSPSGPLHFGSLVAALGSYLQAKSHQGKWLLRIEDLDPPREVSGAASLIIQTIENYGLEWDEEIVYQSQR
HDLYQSQIDKWLQENNAYYCQCTRKQIKEDGGFYLGHCKEKHHSEKNSAVRLVMQHPVESFNDIKHGTITIPSKLAHEDF
IIKRRDGLFAYNLAVVLDDIDQGVTEVVRGADLIEPTGRQISLYRQLGANEVNYLHLPLAVDEKGNKLSKQNYAPAIDNS
NPKPTLLAALTFLGFIIPLEVKQATLNEILQWAIQHWQLNQLPTGLEITTPFSKPSI

Sequences:

>Translated_297_residues
MTTQYIGRFAPSPSGPLHFGSLVAALGSYLQAKSHQGKWLLRIEDLDPPREVSGAASLIIQTIENYGLEWDEEIVYQSQR
HDLYQSQIDKWLQENNAYYCQCTRKQIKEDGGFYLGHCKEKHHSEKNSAVRLVMQHPVESFNDIKHGTITIPSKLAHEDF
IIKRRDGLFAYNLAVVLDDIDQGVTEVVRGADLIEPTGRQISLYRQLGANEVNYLHLPLAVDEKGNKLSKQNYAPAIDNS
NPKPTLLAALTFLGFIIPLEVKQATLNEILQWAIQHWQLNQLPTGLEITTPFSKPSI
>Mature_296_residues
TTQYIGRFAPSPSGPLHFGSLVAALGSYLQAKSHQGKWLLRIEDLDPPREVSGAASLIIQTIENYGLEWDEEIVYQSQRH
DLYQSQIDKWLQENNAYYCQCTRKQIKEDGGFYLGHCKEKHHSEKNSAVRLVMQHPVESFNDIKHGTITIPSKLAHEDFI
IKRRDGLFAYNLAVVLDDIDQGVTEVVRGADLIEPTGRQISLYRQLGANEVNYLHLPLAVDEKGNKLSKQNYAPAIDNSN
PKPTLLAALTFLGFIIPLEVKQATLNEILQWAIQHWQLNQLPTGLEITTPFSKPSI

Specific function: Catalyzes the tRNA-independent activation of glutamate in presence of ATP and the subsequent transfer of glutamate onto a tRNA(Asp). Glutamate is transferred on the 2-amino-5-(4,5- dihydroxy-2-cyclopenten-1-yl) moiety of the queuosine in the wobble positi

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. GluQ subfamily [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=249, Percent_Identity=32.5301204819277, Blast_Score=124, Evalue=1e-28,
Organism=Escherichia coli, GI226510925, Length=295, Percent_Identity=57.2881355932203, Blast_Score=347, Evalue=7e-97,
Organism=Escherichia coli, GI1788743, Length=311, Percent_Identity=34.0836012861736, Blast_Score=147, Evalue=9e-37,
Organism=Caenorhabditis elegans, GI71993394, Length=251, Percent_Identity=29.4820717131474, Blast_Score=98, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6324540, Length=267, Percent_Identity=30.3370786516854, Blast_Score=120, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24665321, Length=254, Percent_Identity=31.496062992126, Blast_Score=116, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022380
- InterPro:   IPR000924
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: NA

Molecular weight: Translated: 33558; Mature: 33426

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQYIGRFAPSPSGPLHFGSLVAALGSYLQAKSHQGKWLLRIEDLDPPREVSGAASLII
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHH
QTIENYGLEWDEEIVYQSQRHDLYQSQIDKWLQENNAYYCQCTRKQIKEDGGFYLGHCKE
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHCCCEEEECCHH
KHHSEKNSAVRLVMQHPVESFNDIKHGTITIPSKLAHEDFIIKRRDGLFAYNLAVVLDDI
HHCCCHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHCHHEEEECCCEEEEHHHHHHHHH
DQGVTEVVRGADLIEPTGRQISLYRQLGANEVNYLHLPLAVDEKGNKLSKQNYAPAIDNS
HHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCC
NPKPTLLAALTFLGFIIPLEVKQATLNEILQWAIQHWQLNQLPTGLEITTPFSKPSI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCC
>Mature Secondary Structure 
TTQYIGRFAPSPSGPLHFGSLVAALGSYLQAKSHQGKWLLRIEDLDPPREVSGAASLII
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHH
QTIENYGLEWDEEIVYQSQRHDLYQSQIDKWLQENNAYYCQCTRKQIKEDGGFYLGHCKE
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHCCCEEEECCHH
KHHSEKNSAVRLVMQHPVESFNDIKHGTITIPSKLAHEDFIIKRRDGLFAYNLAVVLDDI
HHCCCHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHCHHEEEECCCEEEEHHHHHHHHH
DQGVTEVVRGADLIEPTGRQISLYRQLGANEVNYLHLPLAVDEKGNKLSKQNYAPAIDNS
HHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCC
NPKPTLLAALTFLGFIIPLEVKQATLNEILQWAIQHWQLNQLPTGLEITTPFSKPSI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA