Click here to switch to the map view.

The map label for this gene is dapE

Identifier: 58616814

GI number: 58616814

Start: 142263

End: 143414

Strand: Reverse

Name: dapE

Synonym: ERGA_CDS_00870

Alternate gene names: 58616814

Gene position: 143414-142263 (Counterclockwise)

Preceding gene: 58616815

Following gene: 58616813

Centisome position: 9.56

GC content: 31.42

Gene sequence:

>1152_bases
ATGGTTATTGATCCAGTTACATTATCACAAGAATTAATATCATTCCCTAGTATTACACCAACAGATAATGGTGCAATAAG
TTTTCTATCAGATATATTATCACAATATGGTTTTACATGTCATATTCTAGATTTTGGTGATGATACGGTTACAGTACGTA
ACTTGTACGCATACCGAGGAACAGAAGAAGGTCCAAACCTATGTTTTGCTGGACATACAGATGTAGTAAAAACTGGGGAT
TTAACAAAATGGAAATTTGATCCTTTTTCTGGTCATATAGAAGATGATATCTTATATGGCCGTGGTGCTGTTGATATGAA
ATCAGCAATATGTGCATTTATAGCAGCAGTATCTAGGATAAACTTCAATGAAGTTCCAGGTTCCATTAGTTTCCTAATTT
CTGGAGATGAAGAAGGAGACCATTTTCAATATGGTACACCATCAGTATTAAAATGGTTAAATGAAAATAATCATAAAATA
GATTACTGTATAATTGGAGAGCCAACTAGCAAGTCGTTCCTTGGAGACACAATAAAAGTAGGCCGTAGAGGTTCTGTACA
TTTTAAAATAATATGCAATGGCATACAAGGGCATGTTGCATATCCACATTTTGCAGAAAATCCAATAGATAATATGGTGA
GCATTCTTTATAAAATATGTAATACAACATTTGATACTGGAAATGAGTATTTTCAACCATCAAATTGTGAAATTGTATCA
GTTGACACTGGAAATACATCAAGAAATGTAATTCCAGATACAATAGTTGCACACATCAATATCAGGTATAATAATATACA
TACCGCTGAATCACTATTTGACATTATTAATAATATTTGTGCTCAAGTAACACCAAAATATCAATTACTACATTCAGTAA
GTGGTGAACCATTTTTTAATCAGCCTAATCAATATTCTGATATGTTATCTTCAGCTATAAAAAAAGTAACAGGACAAGAT
GCAATAGCAAGTACATCTGGTGGTGTTTCAGATTCAAGATTTATAAAAAATGTTTGTCCTGTAATTGAATTTGGCTTAAA
AAATGAAACAGCACATAAAATTGACGAGCATGTGCCTGTAAAAGAAATTTATCAGTTAGCTGATATATATACAGAATTCA
TTAAGCAATTCTTTAATTTATCAACAACATAA

Upstream 100 bases:

>100_bases
ACCTAAATTCATTATAGTTGTACTACATTACCAATCTTGACAACCATTAGAAATTATACCATAGTAAATTATTAATATTA
TATTTTATTTATTATTACCT

Downstream 100 bases:

>100_bases
GTGTTTAGTAAAATTATAAGTCATATTTTCCCTAAAACATGCGCAGGCTGTGAGTGTATTATCCCTGAATGCTGTGATTT
ATGTAGTACGTGTAGTAATA

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase

Number of amino acids: Translated: 383; Mature: 383

Protein sequence:

>383_residues
MVIDPVTLSQELISFPSITPTDNGAISFLSDILSQYGFTCHILDFGDDTVTVRNLYAYRGTEEGPNLCFAGHTDVVKTGD
LTKWKFDPFSGHIEDDILYGRGAVDMKSAICAFIAAVSRINFNEVPGSISFLISGDEEGDHFQYGTPSVLKWLNENNHKI
DYCIIGEPTSKSFLGDTIKVGRRGSVHFKIICNGIQGHVAYPHFAENPIDNMVSILYKICNTTFDTGNEYFQPSNCEIVS
VDTGNTSRNVIPDTIVAHINIRYNNIHTAESLFDIINNICAQVTPKYQLLHSVSGEPFFNQPNQYSDMLSSAIKKVTGQD
AIASTSGGVSDSRFIKNVCPVIEFGLKNETAHKIDEHVPVKEIYQLADIYTEFIKQFFNLSTT

Sequences:

>Translated_383_residues
MVIDPVTLSQELISFPSITPTDNGAISFLSDILSQYGFTCHILDFGDDTVTVRNLYAYRGTEEGPNLCFAGHTDVVKTGD
LTKWKFDPFSGHIEDDILYGRGAVDMKSAICAFIAAVSRINFNEVPGSISFLISGDEEGDHFQYGTPSVLKWLNENNHKI
DYCIIGEPTSKSFLGDTIKVGRRGSVHFKIICNGIQGHVAYPHFAENPIDNMVSILYKICNTTFDTGNEYFQPSNCEIVS
VDTGNTSRNVIPDTIVAHINIRYNNIHTAESLFDIINNICAQVTPKYQLLHSVSGEPFFNQPNQYSDMLSSAIKKVTGQD
AIASTSGGVSDSRFIKNVCPVIEFGLKNETAHKIDEHVPVKEIYQLADIYTEFIKQFFNLSTT
>Mature_383_residues
MVIDPVTLSQELISFPSITPTDNGAISFLSDILSQYGFTCHILDFGDDTVTVRNLYAYRGTEEGPNLCFAGHTDVVKTGD
LTKWKFDPFSGHIEDDILYGRGAVDMKSAICAFIAAVSRINFNEVPGSISFLISGDEEGDHFQYGTPSVLKWLNENNHKI
DYCIIGEPTSKSFLGDTIKVGRRGSVHFKIICNGIQGHVAYPHFAENPIDNMVSILYKICNTTFDTGNEYFQPSNCEIVS
VDTGNTSRNVIPDTIVAHINIRYNNIHTAESLFDIINNICAQVTPKYQLLHSVSGEPFFNQPNQYSDMLSSAIKKVTGQD
AIASTSGGVSDSRFIKNVCPVIEFGLKNETAHKIDEHVPVKEIYQLADIYTEFIKQFFNLSTT

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily

Homologues:

Organism=Escherichia coli, GI1788816, Length=380, Percent_Identity=36.5789473684211, Blast_Score=251, Evalue=6e-68,
Organism=Escherichia coli, GI1790395, Length=390, Percent_Identity=23.5897435897436, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789236, Length=285, Percent_Identity=21.4035087719298, Blast_Score=68, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPE_EHRRG (Q5FFA0)

Other databases:

- EMBL:   CR925677
- RefSeq:   YP_196013.1
- ProteinModelPortal:   Q5FFA0
- SMR:   Q5FFA0
- STRING:   Q5FFA0
- MEROPS:   M20.010
- GeneID:   3268443
- GenomeReviews:   CR925677_GR
- KEGG:   erg:ERGA_CDS_00870
- eggNOG:   COG0624
- HOGENOM:   HBG728841
- OMA:   PMVFEDT
- ProtClustDB:   PRK13009
- BioCyc:   ERUM302409:ERGA_CDS_00870-MONOMER
- GO:   GO:0006508
- HAMAP:   MF_01690
- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650
- TIGRFAMs:   TIGR01246

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: =3.5.1.18

Molecular weight: Translated: 42486; Mature: 42486

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00758 ARGE_DAPE_CPG2_1; PS00759 ARGE_DAPE_CPG2_2

Important sites: ACT_SITE 74-74 ACT_SITE 137-137

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVIDPVTLSQELISFPSITPTDNGAISFLSDILSQYGFTCHILDFGDDTVTVRNLYAYRG
CEECCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCEEEEEEEEEECC
TEEGPNLCFAGHTDVVKTGDLTKWKFDPFSGHIEDDILYGRGAVDMKSAICAFIAAVSRI
CCCCCCEEEECCCCEEECCCCCEEECCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHC
NFNEVPGSISFLISGDEEGDHFQYGTPSVLKWLNENNHKIDYCIIGEPTSKSFLGDTIKV
CCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHCCCCCEEEEEEECCCCCCHHCCHHHHC
GRRGSVHFKIICNGIQGHVAYPHFAENPIDNMVSILYKICNTTFDTGNEYFQPSNCEIVS
CCCCCEEEEEEEECCCCCEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEE
VDTGNTSRNVIPDTIVAHINIRYNNIHTAESLFDIINNICAQVTPKYQLLHSVSGEPFFN
EECCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCC
QPNQYSDMLSSAIKKVTGQDAIASTSGGVSDSRFIKNVCPVIEFGLKNETAHKIDEHVPV
CCHHHHHHHHHHHHHHCCCCHHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHCCCH
KEIYQLADIYTEFIKQFFNLSTT
HHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MVIDPVTLSQELISFPSITPTDNGAISFLSDILSQYGFTCHILDFGDDTVTVRNLYAYRG
CEECCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCEEEEEEEEEECC
TEEGPNLCFAGHTDVVKTGDLTKWKFDPFSGHIEDDILYGRGAVDMKSAICAFIAAVSRI
CCCCCCEEEECCCCEEECCCCCEEECCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHC
NFNEVPGSISFLISGDEEGDHFQYGTPSVLKWLNENNHKIDYCIIGEPTSKSFLGDTIKV
CCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHCCCCCEEEEEEECCCCCCHHCCHHHHC
GRRGSVHFKIICNGIQGHVAYPHFAENPIDNMVSILYKICNTTFDTGNEYFQPSNCEIVS
CCCCCEEEEEEEECCCCCEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEE
VDTGNTSRNVIPDTIVAHINIRYNNIHTAESLFDIINNICAQVTPKYQLLHSVSGEPFFN
EECCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCC
QPNQYSDMLSSAIKKVTGQDAIASTSGGVSDSRFIKNVCPVIEFGLKNETAHKIDEHVPV
CCHHHHHHHHHHHHHHCCCCHHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHCCCH
KEIYQLADIYTEFIKQFFNLSTT
HHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA