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The map label for this gene is purF [H]

Identifier: 58616810

GI number: 58616810

Start: 136833

End: 138233

Strand: Reverse

Name: purF [H]

Synonym: ERGA_CDS_00830

Alternate gene names: 58616810

Gene position: 138233-136833 (Counterclockwise)

Preceding gene: 58616811

Following gene: 58616806

Centisome position: 9.22

GC content: 30.41

Gene sequence:

>1401_bases
ATGCAATTTAATGAAATATATGAAGAATGTGGAGTATTTGCCATATATAATAACAATTATGCAGCTATCAATTGTATTTT
AGGATTACACGCTATTCAACATAGAGGACAAGAATCATTTGGTATAGTAACATCTGATAATACTAATTTTCACTCCTATT
ACTCTAATGAACAAGTGAATACTATCTTCACTCAACAATCAAAAATAGACCACCTACTAGGAAATATTGCTATTGGACAC
GTGCGTTACTCTACAAGCGGAAGTAAAACTGGTATGCAACCTATAATTTTAGATTGTCAATTTGGAAAATTAGCAATAGC
ACATAATGGTAACCTTATTAATGCATTACAAATTAGAGAATCACTCATTAAAAAAGGACGTATATTTTCATCAGACATTG
ATACAGAAGTAATAGCACATCTTATTGCTATGAATACACAAGATACTATATTGGACAATATAATATACGCTCTACAAAAT
ATTAAGGGTGCTTATTCTCTAGTAATTTTGATAAACAATACTTTAATATGTTGTCGAGATCCATATGGTATTAGACCATT
AGTACTAGGCATGTTAGACAATGTATATGTAATAGCTTCAGAAACATGTGCTTTAGATATAGTTGGGGCACAATTTGTTA
GAGATATTTTACCAGGAGAATTAATAACAATTAATCAAAGTAATACCTTAACAAGTTATTTCCCATTTGAAAAACAAAAA
TCAAGTTTTTGTATTTTTGAATACGTATATTTTGCACGACCAGACAGCATAATGGAAAATAAGTCTGTATATGAAATACG
TAAAAACATAGGAAGAGAACTAGCAATAGAGAATACTGTACCAAAAGATACAAATATGGTAATACCTATACCTGATTCTG
GAGTACCAGCTGCATTAGGGTTTGCAGAATATACTAATATACCTTTTGAGTTCGGCATTATTAGGAATCATTATATAGGA
AGAACTTTTATACAACCTCACGACCATATTCGTAATATAGGAGTAAAGCTTAAGCATAATCCCAATTCTTCAATATTAAA
AGGTAAAAATATAGTTTTAATAGATGATAGTATTGTACGAGGAACAACTTTAAAAAATATAATAGCATTACTCCATAAAG
CAGGTGTCAAACAGATACACTTAAGAATCTCAAGTCCTCCTACTATACATTCTTGCTTCTACGGAATAGACACACCAGAA
GAATCAAAATTGATAGCCAACAATTTATCAAAAGCAGACATTATAAAATTATTAGGATGTGATAGCTTACATTTCTTAAG
CATAAATGGGCTTTACAAGGCAATATGCAATACAAAACGCAATAATGTCCCACAATATTGTGATGCATGCTTCACTGGAG
ATTACCCCATTGGTAAAATAGATACAAATAATGTTTACTAA

Upstream 100 bases:

>100_bases
ATAACAATATAGATAATCTATAATTTCAATATAACTTATTAAACTTTGTGCCTTTCTATGCTAGAATATTGACAATAAAA
TTCTAACCATTAAATTAACC

Downstream 100 bases:

>100_bases
ATTACCTCACTACAGTAGTACTTCGTCTACAGCTTCTATTAATTAGAAAGTAATGACATCTCATTAGCATTACGCGTTAA
ACTACAAAGTCATATGTAAA

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MQFNEIYEECGVFAIYNNNYAAINCILGLHAIQHRGQESFGIVTSDNTNFHSYYSNEQVNTIFTQQSKIDHLLGNIAIGH
VRYSTSGSKTGMQPIILDCQFGKLAIAHNGNLINALQIRESLIKKGRIFSSDIDTEVIAHLIAMNTQDTILDNIIYALQN
IKGAYSLVILINNTLICCRDPYGIRPLVLGMLDNVYVIASETCALDIVGAQFVRDILPGELITINQSNTLTSYFPFEKQK
SSFCIFEYVYFARPDSIMENKSVYEIRKNIGRELAIENTVPKDTNMVIPIPDSGVPAALGFAEYTNIPFEFGIIRNHYIG
RTFIQPHDHIRNIGVKLKHNPNSSILKGKNIVLIDDSIVRGTTLKNIIALLHKAGVKQIHLRISSPPTIHSCFYGIDTPE
ESKLIANNLSKADIIKLLGCDSLHFLSINGLYKAICNTKRNNVPQYCDACFTGDYPIGKIDTNNVY

Sequences:

>Translated_466_residues
MQFNEIYEECGVFAIYNNNYAAINCILGLHAIQHRGQESFGIVTSDNTNFHSYYSNEQVNTIFTQQSKIDHLLGNIAIGH
VRYSTSGSKTGMQPIILDCQFGKLAIAHNGNLINALQIRESLIKKGRIFSSDIDTEVIAHLIAMNTQDTILDNIIYALQN
IKGAYSLVILINNTLICCRDPYGIRPLVLGMLDNVYVIASETCALDIVGAQFVRDILPGELITINQSNTLTSYFPFEKQK
SSFCIFEYVYFARPDSIMENKSVYEIRKNIGRELAIENTVPKDTNMVIPIPDSGVPAALGFAEYTNIPFEFGIIRNHYIG
RTFIQPHDHIRNIGVKLKHNPNSSILKGKNIVLIDDSIVRGTTLKNIIALLHKAGVKQIHLRISSPPTIHSCFYGIDTPE
ESKLIANNLSKADIIKLLGCDSLHFLSINGLYKAICNTKRNNVPQYCDACFTGDYPIGKIDTNNVY
>Mature_466_residues
MQFNEIYEECGVFAIYNNNYAAINCILGLHAIQHRGQESFGIVTSDNTNFHSYYSNEQVNTIFTQQSKIDHLLGNIAIGH
VRYSTSGSKTGMQPIILDCQFGKLAIAHNGNLINALQIRESLIKKGRIFSSDIDTEVIAHLIAMNTQDTILDNIIYALQN
IKGAYSLVILINNTLICCRDPYGIRPLVLGMLDNVYVIASETCALDIVGAQFVRDILPGELITINQSNTLTSYFPFEKQK
SSFCIFEYVYFARPDSIMENKSVYEIRKNIGRELAIENTVPKDTNMVIPIPDSGVPAALGFAEYTNIPFEFGIIRNHYIG
RTFIQPHDHIRNIGVKLKHNPNSSILKGKNIVLIDDSIVRGTTLKNIIALLHKAGVKQIHLRISSPPTIHSCFYGIDTPE
ESKLIANNLSKADIIKLLGCDSLHFLSINGLYKAICNTKRNNVPQYCDACFTGDYPIGKIDTNNVY

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=519, Percent_Identity=37.1868978805395, Blast_Score=330, Evalue=1e-90,
Organism=Escherichia coli, GI1788651, Length=456, Percent_Identity=36.6228070175439, Blast_Score=279, Evalue=4e-76,
Organism=Escherichia coli, GI1790167, Length=212, Percent_Identity=29.2452830188679, Blast_Score=80, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17554892, Length=479, Percent_Identity=35.2818371607516, Blast_Score=291, Evalue=7e-79,
Organism=Saccharomyces cerevisiae, GI6323958, Length=457, Percent_Identity=32.3851203501094, Blast_Score=236, Evalue=6e-63,
Organism=Drosophila melanogaster, GI24659598, Length=488, Percent_Identity=39.1393442622951, Blast_Score=326, Evalue=2e-89,
Organism=Drosophila melanogaster, GI24659604, Length=463, Percent_Identity=39.0928725701944, Blast_Score=322, Evalue=4e-88,
Organism=Drosophila melanogaster, GI28573187, Length=502, Percent_Identity=37.6494023904382, Blast_Score=320, Evalue=1e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 52060; Mature: 52060

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFNEIYEECGVFAIYNNNYAAINCILGLHAIQHRGQESFGIVTSDNTNFHSYYSNEQVN
CCHHHHHHHCCEEEEECCCEEEEHHHHHHHHHHHCCCCCCEEEECCCCCEEEEECCCCEE
TIFTQQSKIDHLLGNIAIGHVRYSTSGSKTGMQPIILDCQFGKLAIAHNGNLINALQIRE
EEEECHHHHHHHHHCEEEEEEEEECCCCCCCCCEEEEEECCCEEEEEECCCEEHHHHHHH
SLIKKGRIFSSDIDTEVIAHLIAMNTQDTILDNIIYALQNIKGAYSLVILINNTLICCRD
HHHHCCCEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEEEEECEEEEECC
PYGIRPLVLGMLDNVYVIASETCALDIVGAQFVRDILPGELITINQSNTLTSYFPFEKQK
CCCCHHHHHHHHCCEEEEEECCEEEEHHHHHHHHHHCCCCEEEEECCCCEEEECCCCCCC
SSFCIFEYVYFARPDSIMENKSVYEIRKNIGRELAIENTVPKDTNMVIPIPDSGVPAALG
CCEEEEEEEECCCCCHHHCCCHHHHHHHHCCCEEEECCCCCCCCCEEEECCCCCCCHHCC
FAEYTNIPFEFGIIRNHYIGRTFIQPHDHIRNIGVKLKHNPNSSILKGKNIVLIDDSIVR
HHHHCCCCEEEEEEECCCCCCEECCCCHHHHHCCEEEEECCCCCEECCCEEEEECCCCCC
GTTLKNIIALLHKAGVKQIHLRISSPPTIHSCFYGIDTPEESKLIANNLSKADIIKLLGC
CCHHHHHHHHHHHCCCEEEEEEECCCCCHHHEEECCCCCCCCHHHHHCCCHHHHHHHHCC
DSLHFLSINGLYKAICNTKRNNVPQYCDACFTGDYPIGKIDTNNVY
CCEEEEEECHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQFNEIYEECGVFAIYNNNYAAINCILGLHAIQHRGQESFGIVTSDNTNFHSYYSNEQVN
CCHHHHHHHCCEEEEECCCEEEEHHHHHHHHHHHCCCCCCEEEECCCCCEEEEECCCCEE
TIFTQQSKIDHLLGNIAIGHVRYSTSGSKTGMQPIILDCQFGKLAIAHNGNLINALQIRE
EEEECHHHHHHHHHCEEEEEEEEECCCCCCCCCEEEEEECCCEEEEEECCCEEHHHHHHH
SLIKKGRIFSSDIDTEVIAHLIAMNTQDTILDNIIYALQNIKGAYSLVILINNTLICCRD
HHHHCCCEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEEEEECEEEEECC
PYGIRPLVLGMLDNVYVIASETCALDIVGAQFVRDILPGELITINQSNTLTSYFPFEKQK
CCCCHHHHHHHHCCEEEEEECCEEEEHHHHHHHHHHCCCCEEEEECCCCEEEECCCCCCC
SSFCIFEYVYFARPDSIMENKSVYEIRKNIGRELAIENTVPKDTNMVIPIPDSGVPAALG
CCEEEEEEEECCCCCHHHCCCHHHHHHHHCCCEEEECCCCCCCCCEEEECCCCCCCHHCC
FAEYTNIPFEFGIIRNHYIGRTFIQPHDHIRNIGVKLKHNPNSSILKGKNIVLIDDSIVR
HHHHCCCCEEEEEEECCCCCCEECCCCHHHHHCCEEEEECCCCCEECCCEEEEECCCCCC
GTTLKNIIALLHKAGVKQIHLRISSPPTIHSCFYGIDTPEESKLIANNLSKADIIKLLGC
CCHHHHHHHHHHHCCCEEEEEEECCCCCHHHEEECCCCCCCCHHHHHCCCHHHHHHHHCC
DSLHFLSINGLYKAICNTKRNNVPQYCDACFTGDYPIGKIDTNNVY
CCEEEEEECHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]