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The map label for this gene is greA [H]

Identifier: 58616800

GI number: 58616800

Start: 121007

End: 121507

Strand: Reverse

Name: greA [H]

Synonym: ERGA_CDS_00730

Alternate gene names: 58616800

Gene position: 121507-121007 (Counterclockwise)

Preceding gene: 58616801

Following gene: 58616799

Centisome position: 8.1

GC content: 33.93

Gene sequence:

>501_bases
ATGAATGACTATATGAATGAAAGATTCCCTATTACAAAGGCTGGATTTGAAAAGCTTGAACTAGAGCTGGAGTCATTGAA
ATATGAAAGACCTCAAATTATTAAGTCTATTTCTGAAGCACGGGAATTAGGGGATTTATCTGAAAACGCAGAGTATCATG
CTGCTCGTGAAAGACAAGGGTTAGTAGAAAGTAAAATAATGGAACTGGAATCAAAAAAATCCAGAGCAGAAGTTATAGAT
GTTGCTAACCTATCTGGTGATACCGTAATGTTCGGTGCAACTGTAACAATGTCTATATATGATGAGCAGACTGGTAGTAC
CGATGAAGTTATATATCAGATTGTTGGTGAATATGAATCTGACATTTCTAAGCGTATGATTTCAATCAAGTCTCCTTTAG
TTATATCGTTACTTGGTAAAAAAGAAGGTGATGTTGTAGAGGTAAAAACTCCAAAAGGTGATTACAGAATATACAAAATT
ATCAAGATTGAGTTTATATAA

Upstream 100 bases:

>100_bases
ATTTTACTGATGACATCAGGTCTTTATTGTCAAAAGTTATAAGTAAGATTGCTTCTAGCTTAAAGTAATTCTTTATAATT
TTAGAATTTAGGTATGGATT

Downstream 100 bases:

>100_bases
GAGAAAAGTAGAGGAGTGAATGGGTATCATTGTATCAGAAAATCCAAGTTTTATATCTTCTATAGAAGATATAATTGAAG
ATGCTAAAGCAGGTAAAGTA

Product: transcription elongation factor greA

Products: NA

Alternate protein names: Transcript cleavage factor greA [H]

Number of amino acids: Translated: 166; Mature: 166

Protein sequence:

>166_residues
MNDYMNERFPITKAGFEKLELELESLKYERPQIIKSISEARELGDLSENAEYHAARERQGLVESKIMELESKKSRAEVID
VANLSGDTVMFGATVTMSIYDEQTGSTDEVIYQIVGEYESDISKRMISIKSPLVISLLGKKEGDVVEVKTPKGDYRIYKI
IKIEFI

Sequences:

>Translated_166_residues
MNDYMNERFPITKAGFEKLELELESLKYERPQIIKSISEARELGDLSENAEYHAARERQGLVESKIMELESKKSRAEVID
VANLSGDTVMFGATVTMSIYDEQTGSTDEVIYQIVGEYESDISKRMISIKSPLVISLLGKKEGDVVEVKTPKGDYRIYKI
IKIEFI
>Mature_166_residues
MNDYMNERFPITKAGFEKLELELESLKYERPQIIKSISEARELGDLSENAEYHAARERQGLVESKIMELESKKSRAEVID
VANLSGDTVMFGATVTMSIYDEQTGSTDEVIYQIVGEYESDISKRMISIKSPLVISLLGKKEGDVVEVKTPKGDYRIYKI
IKIEFI

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082225, Length=160, Percent_Identity=48.75, Blast_Score=140, Evalue=4e-35,
Organism=Escherichia coli, GI87082261, Length=157, Percent_Identity=33.7579617834395, Blast_Score=76, Evalue=1e-15,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 18881; Mature: 18881

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS00829 GREAB_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDYMNERFPITKAGFEKLELELESLKYERPQIIKSISEARELGDLSENAEYHAARERQG
CCCCCCCCCCCCHHCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
LVESKIMELESKKSRAEVIDVANLSGDTVMFGATVTMSIYDEQTGSTDEVIYQIVGEYES
HHHHHHHHHHHHHHHHHEEEEECCCCCEEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHH
DISKRMISIKSPLVISLLGKKEGDVVEVKTPKGDYRIYKIIKIEFI
HHHHHHHHCCCCEEEEEECCCCCCEEEEECCCCCEEEEEEEEEEEC
>Mature Secondary Structure
MNDYMNERFPITKAGFEKLELELESLKYERPQIIKSISEARELGDLSENAEYHAARERQG
CCCCCCCCCCCCHHCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
LVESKIMELESKKSRAEVIDVANLSGDTVMFGATVTMSIYDEQTGSTDEVIYQIVGEYES
HHHHHHHHHHHHHHHHHEEEEECCCCCEEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHH
DISKRMISIKSPLVISLLGKKEGDVVEVKTPKGDYRIYKIIKIEFI
HHHHHHHHCCCCEEEEEECCCCCCEEEEECCCCCEEEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA