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The map label for this gene is levE [H]

Identifier: 56965829

GI number: 56965829

Start: 4248011

End: 4248490

Strand: Direct

Name: levE [H]

Synonym: ABC4071

Alternate gene names: 56965829

Gene position: 4248011-4248490 (Clockwise)

Preceding gene: 56965828

Following gene: 56965830

Centisome position: 98.7

GC content: 40.83

Gene sequence:

>480_bases
ATGATGGTTACATTTTTTAGAGTTGATGATCGATTGATTCATGGACAAGTAGCGTATTCTTGGACAAAACATTTAGAAGC
AGAAGTCATTCTAGTGGCAAACGACAAAGTTGTGAACGACCCGATTCAAAAAATGTCTTTAAATTTGGCAAAGCCTAGTG
GAGTCGCAGTTGAAATTGCTAGCGTTGCCGACGCCGTTAAGTATATCCAATCGACCGAGCATCAGAACAAGCGTATTTTT
GTGCTAGTAAACAACTCAACAGATGCCTTAACTTTTGCCAAAAGTAATGTCGGCTTACAAAGCATCAACCTAGGCGGAAT
GCGTGCTGGCGAGGGGAAACAGTCGGTTTCTCGTGCGATCTATGTAAACGATCAAGATATTCAACACTTTAAAGAGATTG
AGGAACTAGGGATAGAAGTGGAAATTCGCCAAGTACCTACCGATAGCAAAAAAATATTCTCCAAGGTAGTTAACAGCTAG

Upstream 100 bases:

>100_bases
GAGTCAATTAATAACTGTATTGAGCAAGGCAAGAATGGAATTATATTTGTGAATCAAATGATAGAAGAACATGCTAACTC
ATAGTCGAAGGGAGCAATGA

Downstream 100 bases:

>100_bases
TAAAGGGAGGGGATTAGTATCGATGCTACATTAATTTTACAAGCCACTGTTATTGGCTTGCTTGGTGCATTGGGAATATT
AGATGGAAGAATATTGGGTC

Product: PTS system, mannose-specific enzyme II, B component

Products: NA

Alternate protein names: EIIB-Sor; EIII-B-Sor; PTS system sorbose-specific EIIB component [H]

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MMVTFFRVDDRLIHGQVAYSWTKHLEAEVILVANDKVVNDPIQKMSLNLAKPSGVAVEIASVADAVKYIQSTEHQNKRIF
VLVNNSTDALTFAKSNVGLQSINLGGMRAGEGKQSVSRAIYVNDQDIQHFKEIEELGIEVEIRQVPTDSKKIFSKVVNS

Sequences:

>Translated_159_residues
MMVTFFRVDDRLIHGQVAYSWTKHLEAEVILVANDKVVNDPIQKMSLNLAKPSGVAVEIASVADAVKYIQSTEHQNKRIF
VLVNNSTDALTFAKSNVGLQSINLGGMRAGEGKQSVSRAIYVNDQDIQHFKEIEELGIEVEIRQVPTDSKKIFSKVVNS
>Mature_159_residues
MMVTFFRVDDRLIHGQVAYSWTKHLEAEVILVANDKVVNDPIQKMSLNLAKPSGVAVEIASVADAVKYIQSTEHQNKRIF
VLVNNSTDALTFAKSNVGLQSINLGGMRAGEGKQSVSRAIYVNDQDIQHFKEIEELGIEVEIRQVPTDSKKIFSKVVNS

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG3444

COG function: function code G; Phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IIB

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-4 domain [H]

Homologues:

Organism=Escherichia coli, GI1788120, Length=150, Percent_Identity=37.3333333333333, Blast_Score=116, Evalue=6e-28,
Organism=Escherichia coli, GI87082217, Length=147, Percent_Identity=31.9727891156463, Blast_Score=96, Evalue=1e-21,
Organism=Escherichia coli, GI1789527, Length=147, Percent_Identity=31.2925170068027, Blast_Score=79, Evalue=2e-16,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004720
- InterPro:   IPR018455 [H]

Pfam domain/function: PF03830 PTSIIB_sorb [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 17671; Mature: 17671

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS51101 PTS_EIIB_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMVTFFRVDDRLIHGQVAYSWTKHLEAEVILVANDKVVNDPIQKMSLNLAKPSGVAVEIA
CEEEEEECCCEEEECCEEEHHHHCCCEEEEEEECCCCCCCCHHHHEEECCCCCCCEEEHH
SVADAVKYIQSTEHQNKRIFVLVNNSTDALTFAKSNVGLQSINLGGMRAGEGKQSVSRAI
HHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEECCCCEEEECCCCEECCCCHHHHCEEE
YVNDQDIQHFKEIEELGIEVEIRQVPTDSKKIFSKVVNS
EECCHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCC
>Mature Secondary Structure
MMVTFFRVDDRLIHGQVAYSWTKHLEAEVILVANDKVVNDPIQKMSLNLAKPSGVAVEIA
CEEEEEECCCEEEECCEEEHHHHCCCEEEEEEECCCCCCCCHHHHEEECCCCCCCEEEHH
SVADAVKYIQSTEHQNKRIFVLVNNSTDALTFAKSNVGLQSINLGGMRAGEGKQSVSRAI
HHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEECCCCEEEECCCCEECCCCHHHHCEEE
YVNDQDIQHFKEIEELGIEVEIRQVPTDSKKIFSKVVNS
EECCHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7947968 [H]