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The map label for this gene is levF [H]

Identifier: 56965830

GI number: 56965830

Start: 4248584

End: 4249264

Strand: Direct

Name: levF [H]

Synonym: ABC4072

Alternate gene names: 56965830

Gene position: 4248584-4249264 (Clockwise)

Preceding gene: 56965829

Following gene: 56965831

Centisome position: 98.72

GC content: 47.58

Gene sequence:

>681_bases
TTGGGTCAATCTATGTTTGAAAGACCCATTGTTATGGGCCCACTTGTTGGGCTGGTCATGGGAGACTTTGTCATGGGTAT
TATCGTCGGTGCCACTCTTGAACTTGTCTGGATGGGGATTGTAGGGGTTGGATCAGCAACGCCCCCGGACACAGTAACCG
GCGGCATTCTTGGAACTGCCTATGCAATTATGACAGGGGAAGGACCGGAAGTTGCTCTTGCACTAGCGATCCCTATCGCA
TTGCTGGCACAGTCACTTGGTATGCTTGTGCGTACGATTAATGCTCCGCTTATTCACAAGGCAGACCGTTATGCGCAATC
AGGAAATTTCAAAAAGGTAGCCAGTCTCCATTGGTTAGGCGTCTTCTTTTTCTTCCTAAACGGTTTTATACCTAGTTTCC
TTGGGATTCTAGTAGGGTCAGAAGTCGTAAATGTCATCATTGAGTGGATTCCGGCAGTTGTTACCGATGGACTCGGCGCA
GCAGCAGCTATGTTCCCTGCTCTTGGGTTCGCGTTGCTCATGCAGATGACATTTTCGAAAAAATTGGCGCCATTTTTCTT
TTTAGGATTTGTCCTCGCTGTCTTTTTAGGGCTAGATATTATTCCGGTCGCTATTATTGGCGCGATCATTGCCACAGTTA
TGTACCAGCTTCAACAATCTAATGAGGGGGCTGCGACCTAA

Upstream 100 bases:

>100_bases
CAGCTAGTAAAGGGAGGGGATTAGTATCGATGCTACATTAATTTTACAAGCCACTGTTATTGGCTTGCTTGGTGCATTGG
GAATATTAGATGGAAGAATA

Downstream 100 bases:

>100_bases
TGGAACCACTAAAAGAAACGGTTGAGCCGAGTGCTCAGCCAGAACTACAAGTTACAAAGAAAGACTTGCGTAAAGTGTTT
TGGCGTTCGTTTACCTTGCA

Product: PTS system, mannose-specific enzyme II, C component

Products: protein histidine; sugar phosphate; D-glucosamine-6-phosphate [Cytoplasm]; pyruvate; glucose-6-phosphate [Cytoplasm]; N-acetyl-D-glucosamine-6-phosphate [Cytoplasm]; mannose-6-phosphate [Cytoplasm]; fructose-6-phosphate [Cytoplasm] [C]

Alternate protein names: EIIC-Sor; PTS system sorbose-specific EIIC component [H]

Number of amino acids: Translated: 226; Mature: 225

Protein sequence:

>226_residues
MGQSMFERPIVMGPLVGLVMGDFVMGIIVGATLELVWMGIVGVGSATPPDTVTGGILGTAYAIMTGEGPEVALALAIPIA
LLAQSLGMLVRTINAPLIHKADRYAQSGNFKKVASLHWLGVFFFFLNGFIPSFLGILVGSEVVNVIIEWIPAVVTDGLGA
AAAMFPALGFALLMQMTFSKKLAPFFFLGFVLAVFLGLDIIPVAIIGAIIATVMYQLQQSNEGAAT

Sequences:

>Translated_226_residues
MGQSMFERPIVMGPLVGLVMGDFVMGIIVGATLELVWMGIVGVGSATPPDTVTGGILGTAYAIMTGEGPEVALALAIPIA
LLAQSLGMLVRTINAPLIHKADRYAQSGNFKKVASLHWLGVFFFFLNGFIPSFLGILVGSEVVNVIIEWIPAVVTDGLGA
AAAMFPALGFALLMQMTFSKKLAPFFFLGFVLAVFLGLDIIPVAIIGAIIATVMYQLQQSNEGAAT
>Mature_225_residues
GQSMFERPIVMGPLVGLVMGDFVMGIIVGATLELVWMGIVGVGSATPPDTVTGGILGTAYAIMTGEGPEVALALAIPIAL
LAQSLGMLVRTINAPLIHKADRYAQSGNFKKVASLHWLGVFFFFLNGFIPSFLGILVGSEVVNVIIEWIPAVVTDGLGAA
AAMFPALGFALLMQMTFSKKLAPFFFLGFVLAVFLGLDIIPVAIIGAIIATVMYQLQQSNEGAAT

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: COG3715

COG function: function code G; Phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IIC

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-4 domain [H]

Homologues:

Organism=Escherichia coli, GI1788121, Length=214, Percent_Identity=35.5140186915888, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1789528, Length=218, Percent_Identity=26.605504587156, Blast_Score=98, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004700
- InterPro:   IPR018404 [H]

Pfam domain/function: PF03609 EII-Sor [H]

EC number: NA

Molecular weight: Translated: 23706; Mature: 23575

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS51106 PTS_EIIC_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQSMFERPIVMGPLVGLVMGDFVMGIIVGATLELVWMGIVGVGSATPPDTVTGGILGTA
CCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
YAIMTGEGPEVALALAIPIALLAQSLGMLVRTINAPLIHKADRYAQSGNFKKVASLHWLG
HHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHH
VFFFFLNGFIPSFLGILVGSEVVNVIIEWIPAVVTDGLGAAAAMFPALGFALLMQMTFSK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
KLAPFFFLGFVLAVFLGLDIIPVAIIGAIIATVMYQLQQSNEGAAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
GQSMFERPIVMGPLVGLVMGDFVMGIIVGATLELVWMGIVGVGSATPPDTVTGGILGTA
CCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
YAIMTGEGPEVALALAIPIALLAQSLGMLVRTINAPLIHKADRYAQSGNFKKVASLHWLG
HHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHH
VFFFFLNGFIPSFLGILVGSEVVNVIIEWIPAVVTDGLGAAAAMFPALGFALLMQMTFSK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
KLAPFFFLGFVLAVFLGLDIIPVAIIGAIIATVMYQLQQSNEGAAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N p -phosphohistidine; sugar; phosphoenolpyruvate; glucosamine [Periplasm]; phosphoenolpyruvate; beta-D-glucose [Periplasm]; N-acetyl-D-glucosamine [Periplasm]; mannose [Periplasm]; fructose [Periplasm] [C]

Specific reaction: protein N p -phosphohistidine + sugar = protein histidine + sugar phosphate phosphoenolpyruvate + glucosamine [Periplasm] = D-glucosamine-6-phosphate [Cytoplasm] + pyruvate phosphoenolpyruvate + beta-D-glucose [Periplasm] = glucose-6-phosphate [Cytoplasm]

General reaction: Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7947968 [H]