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The map label for this gene is rihA [H]

Identifier: 56964969

GI number: 56964969

Start: 3324253

End: 3325185

Strand: Direct

Name: rihA [H]

Synonym: ABC3205

Alternate gene names: 56964969

Gene position: 3324253-3325185 (Clockwise)

Preceding gene: 56964968

Following gene: 56964970

Centisome position: 77.24

GC content: 49.73

Gene sequence:

>933_bases
GTGGCCAGAAAAATCTTACTTGACGTTGATACAGGCGTAGATGATGCCCTCGCCATTATCCTTGCCTCCAAAAGCACGGA
CGCACAACTGCTTGCAATTACGACAGTGAACGGAAATGTTTCTTTAGAAAAAGCGACTGTTAACACGTTAAAAGTGTTGA
CACTTCTTCATAAAGAGGGCGACATTCCCGTTATCCAAGGGGCTCATGCGCCACTTCGGCGCCCGTGCTTTTTTGAACAT
AGCGTCCACGGCAATGACGGCCTTGGCGGTGCTTTGCCTCATTTTGAGCCAACGGCACAGCCTGCCGACGGTTATGCTCC
TGATTATATAATCGAACAGGCAAACCGTTATCCAGGCGAACTCACGCTTGTCATGACAGGGCCGCTGACGAACTTGGCGT
TAGCGCTTGAAAAATGCCCTGATTTGCCAAAACTCGTGGCGGGCGTCGTCTATATGGGTGGTGCCGCCTTTACACATGGC
AATGTCACCCCTGTGGCTGAGTACAACATGTATGTCGATCCTGAAGCAGCGCGCAAGGTGATCCATGCCGGCTTCGCCCC
CCTTGTGCAAGTCGGATTAGATGTTACCCGCAAAGCACTTCTCACTAGGGAAAACATTGGATCGCTCGTTGACTCGCCTA
TCAAGCAGTTTATCAAAACGTGCACCGCAGACTATATAAAACGGTATGAAGCGCGCAACACCGTGGCAGCTTGTGCCCTC
CACGATCCTCTTGCCGTTGCTTATGCCTTAAAACCCGAATTGCTCCATTTGGAAGAGATGTTTGTGGAAGTAGAAACAAA
CAGCCAATTTTGCGATGGGCAGACGGTATGCGACAGGCAACAGCGCTTAGGAAAACCGGCAAACGTTAACGTTGCCACTG
AGATCGACAGCGATGCATTTTTATCGTACTTTTTGCATTCAATTCAAAACTGA

Upstream 100 bases:

>100_bases
TGAGGCGCTAAACCTGCTTGACATGGAATACGTAAACAGCGAATCAGCCGAATGGATTGACCGCTGGAATAAAGAAATTG
CCAACTAAGGAGGCATTGAA

Downstream 100 bases:

>100_bases
ACATGGCCTTAGGCGTCTCGATGCCGGGCCATTCTTTTCTGTGAGGTGATTTGTTCGTGAACCGTTCAATGCGCATGCTT
TTGCTCATTCCAGGGCTGCT

Product: inosine-uridine preferring nucleoside hydrolase

Products: cytosine; ribose; uracil [C]

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MARKILLDVDTGVDDALAIILASKSTDAQLLAITTVNGNVSLEKATVNTLKVLTLLHKEGDIPVIQGAHAPLRRPCFFEH
SVHGNDGLGGALPHFEPTAQPADGYAPDYIIEQANRYPGELTLVMTGPLTNLALALEKCPDLPKLVAGVVYMGGAAFTHG
NVTPVAEYNMYVDPEAARKVIHAGFAPLVQVGLDVTRKALLTRENIGSLVDSPIKQFIKTCTADYIKRYEARNTVAACAL
HDPLAVAYALKPELLHLEEMFVEVETNSQFCDGQTVCDRQQRLGKPANVNVATEIDSDAFLSYFLHSIQN

Sequences:

>Translated_310_residues
MARKILLDVDTGVDDALAIILASKSTDAQLLAITTVNGNVSLEKATVNTLKVLTLLHKEGDIPVIQGAHAPLRRPCFFEH
SVHGNDGLGGALPHFEPTAQPADGYAPDYIIEQANRYPGELTLVMTGPLTNLALALEKCPDLPKLVAGVVYMGGAAFTHG
NVTPVAEYNMYVDPEAARKVIHAGFAPLVQVGLDVTRKALLTRENIGSLVDSPIKQFIKTCTADYIKRYEARNTVAACAL
HDPLAVAYALKPELLHLEEMFVEVETNSQFCDGQTVCDRQQRLGKPANVNVATEIDSDAFLSYFLHSIQN
>Mature_309_residues
ARKILLDVDTGVDDALAIILASKSTDAQLLAITTVNGNVSLEKATVNTLKVLTLLHKEGDIPVIQGAHAPLRRPCFFEHS
VHGNDGLGGALPHFEPTAQPADGYAPDYIIEQANRYPGELTLVMTGPLTNLALALEKCPDLPKLVAGVVYMGGAAFTHGN
VTPVAEYNMYVDPEAARKVIHAGFAPLVQVGLDVTRKALLTRENIGSLVDSPIKQFIKTCTADYIKRYEARNTVAACALH
DPLAVAYALKPELLHLEEMFVEVETNSQFCDGQTVCDRQQRLGKPANVNVATEIDSDAFLSYFLHSIQN

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788486, Length=303, Percent_Identity=39.2739273927393, Blast_Score=192, Evalue=2e-50,
Organism=Escherichia coli, GI1786871, Length=310, Percent_Identity=40.3225806451613, Blast_Score=185, Evalue=3e-48,
Organism=Escherichia coli, GI1786213, Length=303, Percent_Identity=39.2739273927393, Blast_Score=166, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI32565474, Length=190, Percent_Identity=35.2631578947368, Blast_Score=101, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17565698, Length=302, Percent_Identity=29.1390728476821, Blast_Score=88, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6320608, Length=206, Percent_Identity=35.4368932038835, Blast_Score=95, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24641839, Length=327, Percent_Identity=30.5810397553517, Blast_Score=104, Evalue=8e-23,
Organism=Drosophila melanogaster, GI24583915, Length=326, Percent_Identity=27.3006134969325, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24641837, Length=198, Percent_Identity=34.8484848484849, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022975 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: NA

Molecular weight: Translated: 33559; Mature: 33428

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARKILLDVDTGVDDALAIILASKSTDAQLLAITTVNGNVSLEKATVNTLKVLTLLHKEG
CCCEEEEEECCCCCCEEEEEEECCCCCCEEEEEEEECCCEEEEHHHHHHHHHHHHHHCCC
DIPVIQGAHAPLRRPCFFEHSVHGNDGLGGALPHFEPTAQPADGYAPDYIIEQANRYPGE
CCCEEECCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCE
LTLVMTGPLTNLALALEKCPDLPKLVAGVVYMGGAAFTHGNVTPVAEYNMYVDPEAARKV
EEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHCCCEEECCCCCEEEECCEEECHHHHHHH
IHAGFAPLVQVGLDVTRKALLTRENIGSLVDSPIKQFIKTCTADYIKRYEARNTVAACAL
HHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEC
HDPLAVAYALKPELLHLEEMFVEVETNSQFCDGQTVCDRQQRLGKPANVNVATEIDSDAF
CCCHHEEECCCCHHHHHHHHEEEEECCCCCCCCCHHHHHHHHCCCCCCCEEEEECCHHHH
LSYFLHSIQN
HHHHHHHHCC
>Mature Secondary Structure 
ARKILLDVDTGVDDALAIILASKSTDAQLLAITTVNGNVSLEKATVNTLKVLTLLHKEG
CCEEEEEECCCCCCEEEEEEECCCCCCEEEEEEEECCCEEEEHHHHHHHHHHHHHHCCC
DIPVIQGAHAPLRRPCFFEHSVHGNDGLGGALPHFEPTAQPADGYAPDYIIEQANRYPGE
CCCEEECCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCE
LTLVMTGPLTNLALALEKCPDLPKLVAGVVYMGGAAFTHGNVTPVAEYNMYVDPEAARKV
EEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHCCCEEECCCCCEEEECCEEECHHHHHHH
IHAGFAPLVQVGLDVTRKALLTRENIGSLVDSPIKQFIKTCTADYIKRYEARNTVAACAL
HHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEC
HDPLAVAYALKPELLHLEEMFVEVETNSQFCDGQTVCDRQQRLGKPANVNVATEIDSDAF
CCCHHEEECCCCHHHHHHHHEEEEECCCCCCCCCHHHHHHHHCCCCCCCEEEEECCHHHH
LSYFLHSIQN
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: cytidine; H2O; uridine [C]

Specific reaction: cytidine + H2O = cytosine + ribose uridine + H2O = uracil + ribose [C]

General reaction: Pyrimidine-specific nucleoside hydrolase [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA