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The map label for this gene is potD-A [H]

Identifier: 56964968

GI number: 56964968

Start: 3323194

End: 3324240

Strand: Direct

Name: potD-A [H]

Synonym: ABC3204

Alternate gene names: 56964968

Gene position: 3323194-3324240 (Clockwise)

Preceding gene: 56964967

Following gene: 56964969

Centisome position: 77.21

GC content: 43.46

Gene sequence:

>1047_bases
ATGAAAAAACATCTTTCTTGCTTATTTGTAATCACGTCAAGCTTGGTCGCTGTTAGCGCATGCTCCGCTTCAGGGGACGG
CAATGAAAAACCAACAGACCTTGTCATTTCCACTTGGGGATTTGCAGAGGATTTTTTTAAGGAAGAAGTTTACAAGCCAT
TTCAAGAAGAATACAACGTTAATATTGTTGTCGATATCGGCAACAACGCAGAACGTCTTAACAAAATTCGCCAAGGAACT
GCTAATGTAGATGTCGTTTATTTATCTGATTATTACGCCCAGCAAGGCATCGAGGAAGGTTTATTTGCTGAAATCGATCG
CAGCAACATCGAAAATATTGACAACCTTTATGATATAGCGAAAGCCCCTCTTGGCGAAGAATACGGACCAGCCTACACGA
TTGGCCAATTCGGGATTGCTTACAATCCTGAGCAAACGAAAGGCCCAATCGAAAGCTGGAGCGACTTATGGGATGACTCT
CTTGCAGGCAATTTAACGATTCCTTCTATTACATCGACAACAGGGCCAATGTTTTTAGATGCAGCTTCCATCGTAGCAGG
ACAGGAAGAATTTAATGCCGATGCAGCATTTGCAGCCATTGCCGACCTGAAAGGAAATATTGTTAAAGAGTATGATAAAT
CATCCGACTTCGTCAACATGTTTACCCAAGGCGAGATTGCTGCTGGGCCATTTATGGAAATGTATTTTCATGATTTGCAA
GCCGCTGTCCCTGAAGCTGAGTTCGTTACCCCGACAGAAGGTGGCTACGCAGTCATGAATACAGTCAATGTCGTTAAAGA
CAGTGACAACAAAGAATTAGCGGAAGCTTTTATCAATTGGCATTTGAGCAAAGAAGTCCAGGAAGCGTCAGCTAAAGCGA
AAGTCGACTCCCCTGTGAACATCGATGTTGAGTTGTCTGAACAGGAAGCGGAAGGCGTCACTTATGGGGAAGAAACAATT
GAGGCGCTAAACCTGCTTGACATGGAATACGTAAACAGCGAATCAGCCGAATGGATTGACCGCTGGAATAAAGAAATTGC
CAACTAA

Upstream 100 bases:

>100_bases
ATGTTTACATAAGGAAGATGCACCTGTAGATGGCAAATGCTGCTACAGGTGTGTCTTCTTTTTTTCTTTATTAAAAATGT
GTACTAGGAGGACTTTCTAA

Downstream 100 bases:

>100_bases
GGAGGCATTGAAGTGGCCAGAAAAATCTTACTTGACGTTGATACAGGCGTAGATGATGCCCTCGCCATTATCCTTGCCTC
CAAAAGCACGGACGCACAAC

Product: spermidine/putrescine ABC transporter substrate-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MKKHLSCLFVITSSLVAVSACSASGDGNEKPTDLVISTWGFAEDFFKEEVYKPFQEEYNVNIVVDIGNNAERLNKIRQGT
ANVDVVYLSDYYAQQGIEEGLFAEIDRSNIENIDNLYDIAKAPLGEEYGPAYTIGQFGIAYNPEQTKGPIESWSDLWDDS
LAGNLTIPSITSTTGPMFLDAASIVAGQEEFNADAAFAAIADLKGNIVKEYDKSSDFVNMFTQGEIAAGPFMEMYFHDLQ
AAVPEAEFVTPTEGGYAVMNTVNVVKDSDNKELAEAFINWHLSKEVQEASAKAKVDSPVNIDVELSEQEAEGVTYGEETI
EALNLLDMEYVNSESAEWIDRWNKEIAN

Sequences:

>Translated_348_residues
MKKHLSCLFVITSSLVAVSACSASGDGNEKPTDLVISTWGFAEDFFKEEVYKPFQEEYNVNIVVDIGNNAERLNKIRQGT
ANVDVVYLSDYYAQQGIEEGLFAEIDRSNIENIDNLYDIAKAPLGEEYGPAYTIGQFGIAYNPEQTKGPIESWSDLWDDS
LAGNLTIPSITSTTGPMFLDAASIVAGQEEFNADAAFAAIADLKGNIVKEYDKSSDFVNMFTQGEIAAGPFMEMYFHDLQ
AAVPEAEFVTPTEGGYAVMNTVNVVKDSDNKELAEAFINWHLSKEVQEASAKAKVDSPVNIDVELSEQEAEGVTYGEETI
EALNLLDMEYVNSESAEWIDRWNKEIAN
>Mature_348_residues
MKKHLSCLFVITSSLVAVSACSASGDGNEKPTDLVISTWGFAEDFFKEEVYKPFQEEYNVNIVVDIGNNAERLNKIRQGT
ANVDVVYLSDYYAQQGIEEGLFAEIDRSNIENIDNLYDIAKAPLGEEYGPAYTIGQFGIAYNPEQTKGPIESWSDLWDDS
LAGNLTIPSITSTTGPMFLDAASIVAGQEEFNADAAFAAIADLKGNIVKEYDKSSDFVNMFTQGEIAAGPFMEMYFHDLQ
AAVPEAEFVTPTEGGYAVMNTVNVVKDSDNKELAEAFINWHLSKEVQEASAKAKVDSPVNIDVELSEQEAEGVTYGEETI
EALNLLDMEYVNSESAEWIDRWNKEIAN

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

None

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 38359; Mature: 38359

Theoretical pI: Translated: 3.83; Mature: 3.83

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKHLSCLFVITSSLVAVSACSASGDGNEKPTDLVISTWGFAEDFFKEEVYKPFQEEYNV
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHHCHHHHCCCC
NIVVDIGNNAERLNKIRQGTANVDVVYLSDYYAQQGIEEGLFAEIDRSNIENIDNLYDIA
EEEEECCCCHHHHHHHHCCCCCEEEEEECHHHHHCCHHCCHHHHCCHHHHHHHHHHHHHH
KAPLGEEYGPAYTIGQFGIAYNPEQTKGPIESWSDLWDDSLAGNLTIPSITSTTGPMFLD
HCCCCCCCCCCEEEECCCEEECCCCCCCCHHHHHHHHCCCCCCCEECCCCCCCCCCHHHH
AASIVAGQEEFNADAAFAAIADLKGNIVKEYDKSSDFVNMFTQGEIAAGPFMEMYFHDLQ
HHHHHCCHHHHCCHHHHHHHHHHCCCHHHHHCCCCCHHHHHCCCCCCCCHHHHHHHHHHH
AAVPEAEFVTPTEGGYAVMNTVNVVKDSDNKELAEAFINWHLSKEVQEASAKAKVDSPVN
HHCCCCCEECCCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
IDVELSEQEAEGVTYGEETIEALNLLDMEYVNSESAEWIDRWNKEIAN
EEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKHLSCLFVITSSLVAVSACSASGDGNEKPTDLVISTWGFAEDFFKEEVYKPFQEEYNV
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHHCHHHHCCCC
NIVVDIGNNAERLNKIRQGTANVDVVYLSDYYAQQGIEEGLFAEIDRSNIENIDNLYDIA
EEEEECCCCHHHHHHHHCCCCCEEEEEECHHHHHCCHHCCHHHHCCHHHHHHHHHHHHHH
KAPLGEEYGPAYTIGQFGIAYNPEQTKGPIESWSDLWDDSLAGNLTIPSITSTTGPMFLD
HCCCCCCCCCCEEEECCCEEECCCCCCCCHHHHHHHHCCCCCCCEECCCCCCCCCCHHHH
AASIVAGQEEFNADAAFAAIADLKGNIVKEYDKSSDFVNMFTQGEIAAGPFMEMYFHDLQ
HHHHHCCHHHHCCHHHHHHHHHHCCCHHHHHCCCCCHHHHHCCCCCCCCHHHHHHHHHHH
AAVPEAEFVTPTEGGYAVMNTVNVVKDSDNKELAEAFINWHLSKEVQEASAKAKVDSPVN
HHCCCCCEECCCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
IDVELSEQEAEGVTYGEETIEALNLLDMEYVNSESAEWIDRWNKEIAN
EEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]