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The map label for this gene is potB [H]

Identifier: 56964970

GI number: 56964970

Start: 3325242

End: 3326063

Strand: Direct

Name: potB [H]

Synonym: ABC3206

Alternate gene names: 56964970

Gene position: 3325242-3326063 (Clockwise)

Preceding gene: 56964969

Following gene: 56964971

Centisome position: 77.26

GC content: 43.19

Gene sequence:

>822_bases
GTGAACCGTTCAATGCGCATGCTTTTGCTCATTCCAGGGCTGCTGTTTTTAACTGTTTTTATGCTTATTCCACTCGTGCT
CACGATCAGCTCGACATTCGTCGAAAACGGCAGCTTTTCCTTAGACGGTTATTTTCGTTTTTTAGGTGATGCCTACTTTT
TAGAAATTTTATGGACAACTCTTTTCGTCGCTCTATTAACGACTTTTTGCTGCGTCATTCTTGCTTTTCCCGCCGCTTAT
TACATTTCTAAGTTAAATGGCGGCAAAAAAGCAATCATGCTCATGCTCGCCATTTTCCCATTACTCGTCAGCCCAGTTGT
TCGGTCTTTTAGCTGGATGATTATTCTCGGCCGCAATGGTTTGCTTAACGAGTTTCTTTTGGCGACTGGTCTAATAAACG
AGCCATTATCAATTTTATATACGCCAGCCGCTGTCGCAATCGGCCTCATCCATTTATTTTTGCCACTCATGATCGTTACA
TTAGTTGGCGTAATGGAGAACATTGATGCTGATTTGTTTTTAGCTGCCGAAAGCCTTGGTGCCTCAAAAGCAAAAGCATT
CTTAAAAGTGATGGTGCCCCTTTGTGTACCTGGCCTTGTAATTGGCAGCACGCTCGTATTTGTTGGCAGTTTTACTGCCT
ACACGACGCCAGCGCTTCTAGGCGGCCAACAACGGGTCATTTCGACATTTTTATATCAAAATGCGATTACGTTAAATGAT
TGGCATGCTGCTGCGATGATTGCAACGATTATGATTGTCATCACGTTTGTCGTGACCGGTCTTTTCCAAAAAATCGCAGC
GATTTTAAATCCAAGGGGGTAA

Upstream 100 bases:

>100_bases
GCGATGCATTTTTATCGTACTTTTTGCATTCAATTCAAAACTGAACATGGCCTTAGGCGTCTCGATGCCGGGCCATTCTT
TTCTGTGAGGTGATTTGTTC

Downstream 100 bases:

>100_bases
ACAGATGAACCAATCAAAACATATCGGCCTTGGGTTTGTTTCCTTTCTCATATTTTTGTTTTTGCTCGGGCCACTCGTTA
TTATTTCGGTTGCTTCTTTT

Product: spermidine/putrescine ABC transporter permease

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MNRSMRMLLLIPGLLFLTVFMLIPLVLTISSTFVENGSFSLDGYFRFLGDAYFLEILWTTLFVALLTTFCCVILAFPAAY
YISKLNGGKKAIMLMLAIFPLLVSPVVRSFSWMIILGRNGLLNEFLLATGLINEPLSILYTPAAVAIGLIHLFLPLMIVT
LVGVMENIDADLFLAAESLGASKAKAFLKVMVPLCVPGLVIGSTLVFVGSFTAYTTPALLGGQQRVISTFLYQNAITLND
WHAAAMIATIMIVITFVVTGLFQKIAAILNPRG

Sequences:

>Translated_273_residues
MNRSMRMLLLIPGLLFLTVFMLIPLVLTISSTFVENGSFSLDGYFRFLGDAYFLEILWTTLFVALLTTFCCVILAFPAAY
YISKLNGGKKAIMLMLAIFPLLVSPVVRSFSWMIILGRNGLLNEFLLATGLINEPLSILYTPAAVAIGLIHLFLPLMIVT
LVGVMENIDADLFLAAESLGASKAKAFLKVMVPLCVPGLVIGSTLVFVGSFTAYTTPALLGGQQRVISTFLYQNAITLND
WHAAAMIATIMIVITFVVTGLFQKIAAILNPRG
>Mature_273_residues
MNRSMRMLLLIPGLLFLTVFMLIPLVLTISSTFVENGSFSLDGYFRFLGDAYFLEILWTTLFVALLTTFCCVILAFPAAY
YISKLNGGKKAIMLMLAIFPLLVSPVVRSFSWMIILGRNGLLNEFLLATGLINEPLSILYTPAAVAIGLIHLFLPLMIVT
LVGVMENIDADLFLAAESLGASKAKAFLKVMVPLCVPGLVIGSTLVFVGSFTAYTTPALLGGQQRVISTFLYQNAITLND
WHAAAMIATIMIVITFVVTGLFQKIAAILNPRG

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=265, Percent_Identity=30.5660377358491, Blast_Score=145, Evalue=2e-36,
Organism=Escherichia coli, GI1787080, Length=228, Percent_Identity=34.6491228070175, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1787713, Length=227, Percent_Identity=26.8722466960352, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787570, Length=251, Percent_Identity=24.7011952191235, Blast_Score=64, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29728; Mature: 29728

Theoretical pI: Translated: 8.80; Mature: 8.80

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRSMRMLLLIPGLLFLTVFMLIPLVLTISSTFVENGSFSLDGYFRFLGDAYFLEILWTT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
LFVALLTTFCCVILAFPAAYYISKLNGGKKAIMLMLAIFPLLVSPVVRSFSWMIILGRNG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCC
LLNEFLLATGLINEPLSILYTPAAVAIGLIHLFLPLMIVTLVGVMENIDADLFLAAESLG
HHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHC
ASKAKAFLKVMVPLCVPGLVIGSTLVFVGSFTAYTTPALLGGQQRVISTFLYQNAITLND
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEECH
WHAAAMIATIMIVITFVVTGLFQKIAAILNPRG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNRSMRMLLLIPGLLFLTVFMLIPLVLTISSTFVENGSFSLDGYFRFLGDAYFLEILWTT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
LFVALLTTFCCVILAFPAAYYISKLNGGKKAIMLMLAIFPLLVSPVVRSFSWMIILGRNG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCC
LLNEFLLATGLINEPLSILYTPAAVAIGLIHLFLPLMIVTLVGVMENIDADLFLAAESLG
HHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHC
ASKAKAFLKVMVPLCVPGLVIGSTLVFVGSFTAYTTPALLGGQQRVISTFLYQNAITLND
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEECH
WHAAAMIATIMIVITFVVTGLFQKIAAILNPRG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]