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The map label for this gene is nth [H]

Identifier: 55980081

GI number: 55980081

Start: 112318

End: 112980

Strand: Reverse

Name: nth [H]

Synonym: TTHA0112

Alternate gene names: 55980081

Gene position: 112980-112318 (Counterclockwise)

Preceding gene: 55980083

Following gene: 55980080

Centisome position: 6.11

GC content: 70.44

Gene sequence:

>663_bases
GTGGGGGGCGTGGCGTGCCCTAAGGAAGGGCCAAAGGAGAAGAAGGCCCGGGCCCGGGAGGTCTTAAAGGCCCTCAAGGC
CGCCTACCCCGGGGCCCGCACGGAGCTTAGGCACGAGAACCCCTTCCAGCTCCTCGTGGCCACGGTCCTCTCCGCCCAGG
CCACCGATAAAAGCGTGAACGAGGCCACCCCGGCCCTCTTCGCCCGCTTTCCCGACGCCAAGGCCCTGGCGGAGGCCACC
CCCGAGGAGGTGGAGCCCTACATCCGCCGCATCGGGCTTTACCGCACCAAGGCGAAGAACCTCGTGGCCCTGGCGAGGAG
GCTCGTGGAGGAGTACGGGGGAGAGGTGCCCAAGGAGAAGGAGGCCCTCATGCGCCTTCCCGGGGTGGGCTGGAAGACGG
CCACCGTGGTGCTGGGGGCGGCCTTTGGGGTGCCGGGGATCGCCGTGGACACCCACGTGGCCCGCCTCGCCAGGAGGCTC
TGCTTCTCCGAGGCCAAGGCCCCGGAGAGGATCGGGAAGGACCTCGAGGCCCTCTTCCCCAAGGAGGACTGGGTCTTCGT
CCACCACGCCCTCGTCCTCCACGGCCGCTACGTCTGCACCGCAAGGAGGCCCCGCTGCCGGGCCTGCGTCCTTGCGCCCT
ACTGCCCGAGCCGCGAGGCCTGA

Upstream 100 bases:

>100_bases
CTGCGGATCTCCCAGACCTTCCTCCCGTCCACGATGAGGCTGGCGTAGGGTTCCCGGACGATGAGGCCGAGCTTGGGCCT
TTCCACCCCCTCATTGTAGA

Downstream 100 bases:

>100_bases
TCTCGGAGGAGGCCTTCGTGCGCGCGCCCGTCTACCGCTTCCTCCTCGCCGGCTTTCTCTGGTCCTTCGGGGCCAACCTC
GTCTACTTCTTCCTCAACTT

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 220; Mature: 219

Protein sequence:

>220_residues
MGGVACPKEGPKEKKARAREVLKALKAAYPGARTELRHENPFQLLVATVLSAQATDKSVNEATPALFARFPDAKALAEAT
PEEVEPYIRRIGLYRTKAKNLVALARRLVEEYGGEVPKEKEALMRLPGVGWKTATVVLGAAFGVPGIAVDTHVARLARRL
CFSEAKAPERIGKDLEALFPKEDWVFVHHALVLHGRYVCTARRPRCRACVLAPYCPSREA

Sequences:

>Translated_220_residues
MGGVACPKEGPKEKKARAREVLKALKAAYPGARTELRHENPFQLLVATVLSAQATDKSVNEATPALFARFPDAKALAEAT
PEEVEPYIRRIGLYRTKAKNLVALARRLVEEYGGEVPKEKEALMRLPGVGWKTATVVLGAAFGVPGIAVDTHVARLARRL
CFSEAKAPERIGKDLEALFPKEDWVFVHHALVLHGRYVCTARRPRCRACVLAPYCPSREA
>Mature_219_residues
GGVACPKEGPKEKKARAREVLKALKAAYPGARTELRHENPFQLLVATVLSAQATDKSVNEATPALFARFPDAKALAEATP
EEVEPYIRRIGLYRTKAKNLVALARRLVEEYGGEVPKEKEALMRLPGVGWKTATVVLGAAFGVPGIAVDTHVARLARRLC
FSEAKAPERIGKDLEALFPKEDWVFVHHALVLHGRYVCTARRPRCRACVLAPYCPSREA

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=182, Percent_Identity=34.0659340659341, Blast_Score=105, Evalue=2e-23,
Organism=Homo sapiens, GI190358497, Length=192, Percent_Identity=30.2083333333333, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI6912520, Length=192, Percent_Identity=30.2083333333333, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI115298654, Length=192, Percent_Identity=30.2083333333333, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI115298652, Length=192, Percent_Identity=30.2083333333333, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI115298650, Length=192, Percent_Identity=30.2083333333333, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI115298648, Length=192, Percent_Identity=30.2083333333333, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1787920, Length=206, Percent_Identity=46.6019417475728, Blast_Score=186, Evalue=1e-48,
Organism=Escherichia coli, GI1789331, Length=183, Percent_Identity=26.775956284153, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17554540, Length=185, Percent_Identity=37.8378378378378, Blast_Score=115, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6324530, Length=212, Percent_Identity=28.3018867924528, Blast_Score=87, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6319304, Length=175, Percent_Identity=26.8571428571429, Blast_Score=71, Evalue=1e-13,
Organism=Drosophila melanogaster, GI45550361, Length=182, Percent_Identity=35.1648351648352, Blast_Score=105, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 24178; Mature: 24047

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: PS00764 ENDONUCLEASE_III_1 ; PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGVACPKEGPKEKKARAREVLKALKAAYPGARTELRHENPFQLLVATVLSAQATDKSVN
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHHCCCHHH
EATPALFARFPDAKALAEATPEEVEPYIRRIGLYRTKAKNLVALARRLVEEYGGEVPKEK
HHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
EALMRLPGVGWKTATVVLGAAFGVPGIAVDTHVARLARRLCFSEAKAPERIGKDLEALFP
HHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHCC
KEDWVFVHHALVLHGRYVCTARRPRCRACVLAPYCPSREA
CCCHHHHHHHHHHHCCCEEECCCCCCCEEEECCCCCCCCC
>Mature Secondary Structure 
GGVACPKEGPKEKKARAREVLKALKAAYPGARTELRHENPFQLLVATVLSAQATDKSVN
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHHCCCHHH
EATPALFARFPDAKALAEATPEEVEPYIRRIGLYRTKAKNLVALARRLVEEYGGEVPKEK
HHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
EALMRLPGVGWKTATVVLGAAFGVPGIAVDTHVARLARRLCFSEAKAPERIGKDLEALFP
HHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHCC
KEDWVFVHHALVLHGRYVCTARRPRCRACVLAPYCPSREA
CCCHHHHHHHHHHHCCCEEECCCCCCCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]