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The map label for this gene is nqo8

Identifier: 55980060

GI number: 55980060

Start: 90732

End: 91829

Strand: Direct

Name: nqo8

Synonym: TTHA0091

Alternate gene names: 55980060

Gene position: 90732-91829 (Clockwise)

Preceding gene: 55980059

Following gene: 55980061

Centisome position: 4.91

GC content: 65.57

Gene sequence:

>1098_bases
GTGACCTGGTCCTACCCCGTAGACCCTTACTGGATGGTGGCCCTGAAGGCCCTCTTGGTGGTGGTGGGCCTCCTCACCGC
CTTCGCCTTCATGACCCTCATTGAGCGGAGGCTCCTCGCCCGCTTCCAGGTGCGCATGGGGCCGAACCGGGTGGGGCCTT
TTGGCCTCCTCCAGCCCCTCGCCGACGCCATCAAGAGCATCTTCAAGGAAGACATCGTGGTGGCCCAGGCGGACCGGTTC
CTCTTCGTCCTCGCCCCCCTCATCTCCGTGGTCTTCGCCCTCCTCGCCTTCGGGCTCATCCCCTTCGGCCCGCCGGGAAG
CTTCTTCGGCTACCAGCCCTGGGTGATCAACCTGGACCTGGGGATCCTCTACCTCTTCGCCGTAAGCGAGCTCGCCGTCT
ACGGGATCTTCCTCTCCGGGTGGGCCTCGGGGAGCAAGTACAGCCTCTTGGGCTCCCTCCGCTCCTCGGCGAGCCTCATC
TCCTACGAGCTCGGCCTGGGCCTCGCCCTCCTCGCCCCCGTGCTCCTCGTGGGCAGCCTCAACCTGAACGACATCGTGAA
CTGGCAGAAGGAGCACGGCTGGCTCTTCCTCTACGCCTTCCCCGCCTTCTTGGTCTACCTCATCGCCAGCATGGCCGAGG
CCGCCCGCACCCCCTTTGACCTCCCCGAGGCGGAGCAGGAGCTCGTGGGCGGGTACCACACGGAGTACAGCTCCATCAAG
TGGGCCCTCTTCCAGATGGCGGAGTACATCCACTTCATCACCGCAAGCGCCCTCATCCCCACCCTCTTCCTGGGCGGCTG
GACCATGCCCGTCCTCGAGGTCCCCTACCTCTGGATGTTCCTCAAGATCGCCTTCTTCCTCTTCTTCTTCATCTGGATCC
GGGCCACCTGGTTCCGCCTGCGCTACGACCAGCTCCTCCGCTTCGGCTGGGGCTTCCTCTTCCCGCTGGCCCTCCTCTGG
TTCCTCGTCACCGCCCTCGTGGTGGCCCTGGACCTCCCCAGGACCTACCTCCTCTACCTCTCCGCCCTAAGCTTCCTCGT
CCTCCTGGGAGCGGTCCTCTACACCCCCAAGCCCGCCCGCAAAGGAGGTGGCGCATGA

Upstream 100 bases:

>100_bases
GGGAGGACGTGCCCAAAGGCCACCTCTACCTCTCGGCCCTGGGGCCCGCGGCGGGCCTCCGGGTGGAGGGGCGCGTCCTG
GTGCCCGCGGGAGGTGAAGC

Downstream 100 bases:

>100_bases
CCCTGAAGGCCCTCGCCCAAAGCCTCGGCATCACCCTGAAGTACCTCTTCTCCAAGCCGGTGACCGTCCCCTACCCCGAC
GCCCCCGTGGCCCTGAAGCC

Product: NADH-quinone oxidoreductase subunit 8

Products: NA

Alternate protein names: NADH dehydrogenase I subunit 8; NDH-1 subunit 8

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MTWSYPVDPYWMVALKALLVVVGLLTAFAFMTLIERRLLARFQVRMGPNRVGPFGLLQPLADAIKSIFKEDIVVAQADRF
LFVLAPLISVVFALLAFGLIPFGPPGSFFGYQPWVINLDLGILYLFAVSELAVYGIFLSGWASGSKYSLLGSLRSSASLI
SYELGLGLALLAPVLLVGSLNLNDIVNWQKEHGWLFLYAFPAFLVYLIASMAEAARTPFDLPEAEQELVGGYHTEYSSIK
WALFQMAEYIHFITASALIPTLFLGGWTMPVLEVPYLWMFLKIAFFLFFFIWIRATWFRLRYDQLLRFGWGFLFPLALLW
FLVTALVVALDLPRTYLLYLSALSFLVLLGAVLYTPKPARKGGGA

Sequences:

>Translated_365_residues
MTWSYPVDPYWMVALKALLVVVGLLTAFAFMTLIERRLLARFQVRMGPNRVGPFGLLQPLADAIKSIFKEDIVVAQADRF
LFVLAPLISVVFALLAFGLIPFGPPGSFFGYQPWVINLDLGILYLFAVSELAVYGIFLSGWASGSKYSLLGSLRSSASLI
SYELGLGLALLAPVLLVGSLNLNDIVNWQKEHGWLFLYAFPAFLVYLIASMAEAARTPFDLPEAEQELVGGYHTEYSSIK
WALFQMAEYIHFITASALIPTLFLGGWTMPVLEVPYLWMFLKIAFFLFFFIWIRATWFRLRYDQLLRFGWGFLFPLALLW
FLVTALVVALDLPRTYLLYLSALSFLVLLGAVLYTPKPARKGGGA
>Mature_364_residues
TWSYPVDPYWMVALKALLVVVGLLTAFAFMTLIERRLLARFQVRMGPNRVGPFGLLQPLADAIKSIFKEDIVVAQADRFL
FVLAPLISVVFALLAFGLIPFGPPGSFFGYQPWVINLDLGILYLFAVSELAVYGIFLSGWASGSKYSLLGSLRSSASLIS
YELGLGLALLAPVLLVGSLNLNDIVNWQKEHGWLFLYAFPAFLVYLIASMAEAARTPFDLPEAEQELVGGYHTEYSSIKW
ALFQMAEYIHFITASALIPTLFLGGWTMPVLEVPYLWMFLKIAFFLFFFIWIRATWFRLRYDQLLRFGWGFLFPLALLWF
LVTALVVALDLPRTYLLYLSALSFLVLLGAVLYTPKPARKGGGA

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every

COG id: COG1005

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 1 (chain H)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 1 family

Homologues:

Organism=Homo sapiens, GI251831107, Length=314, Percent_Identity=41.0828025477707, Blast_Score=207, Evalue=2e-53,
Organism=Escherichia coli, GI1788618, Length=330, Percent_Identity=47.2727272727273, Blast_Score=273, Evalue=1e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NQO8_THET8 (Q60019)

Other databases:

- EMBL:   U52917
- EMBL:   AP008226
- PIR:   T11905
- RefSeq:   YP_143357.1
- ProteinModelPortal:   Q60019
- STRING:   Q60019
- GeneID:   3169634
- GenomeReviews:   AP008226_GR
- KEGG:   ttj:TTHA0091
- NMPDR:   fig|300852.3.peg.122
- eggNOG:   COG1005
- HOGENOM:   HBG727670
- OMA:   SYEAAMG
- ProtClustDB:   CLSK445100
- BioCyc:   TTHE300852:TTHA0091-MONOMER
- HAMAP:   MF_01350
- InterPro:   IPR023020
- InterPro:   IPR001694
- InterPro:   IPR018086
- PANTHER:   PTHR11432

Pfam domain/function: PF00146 NADHdh

EC number: =1.6.99.5

Molecular weight: Translated: 41009; Mature: 40878

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: PS00667 COMPLEX1_ND1_1; PS00668 COMPLEX1_ND1_2

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1243fd2c)-; HASH(0x12afa718)-; HASH(0x12a96dc4)-; HASH(0x12cde384)-; HASH(0x12ab9998)-; HASH(0x12acb65c)-; HASH(0x12aca390)-; HASH(0x12a739d8)-; HASH(0x124522b8)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTWSYPVDPYWMVALKALLVVVGLLTAFAFMTLIERRLLARFQVRMGPNRVGPFGLLQPL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
ADAIKSIFKEDIVVAQADRFLFVLAPLISVVFALLAFGLIPFGPPGSFFGYQPWVINLDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEHHH
GILYLFAVSELAVYGIFLSGWASGSKYSLLGSLRSSASLISYELGLGLALLAPVLLVGSL
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NLNDIVNWQKEHGWLFLYAFPAFLVYLIASMAEAARTPFDLPEAEQELVGGYHTEYSSIK
CHHHHHCHHHCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCHHHHHHHHH
WALFQMAEYIHFITASALIPTLFLGGWTMPVLEVPYLWMFLKIAFFLFFFIWIRATWFRL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RYDQLLRFGWGFLFPLALLWFLVTALVVALDLPRTYLLYLSALSFLVLLGAVLYTPKPAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
KGGGA
CCCCH
>Mature Secondary Structure 
TWSYPVDPYWMVALKALLVVVGLLTAFAFMTLIERRLLARFQVRMGPNRVGPFGLLQPL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
ADAIKSIFKEDIVVAQADRFLFVLAPLISVVFALLAFGLIPFGPPGSFFGYQPWVINLDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEHHH
GILYLFAVSELAVYGIFLSGWASGSKYSLLGSLRSSASLISYELGLGLALLAPVLLVGSL
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NLNDIVNWQKEHGWLFLYAFPAFLVYLIASMAEAARTPFDLPEAEQELVGGYHTEYSSIK
CHHHHHCHHHCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCHHHHHHHHH
WALFQMAEYIHFITASALIPTLFLGGWTMPVLEVPYLWMFLKIAFFLFFFIWIRATWFRL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RYDQLLRFGWGFLFPLALLWFLVTALVVALDLPRTYLLYLSALSFLVLLGAVLYTPKPAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
KGGGA
CCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9020134