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The map label for this gene is nqo3

Identifier: 55980059

GI number: 55980059

Start: 88384

End: 90735

Strand: Direct

Name: nqo3

Synonym: TTHA0090

Alternate gene names: 55980059

Gene position: 88384-90735 (Clockwise)

Preceding gene: 55980058

Following gene: 55980060

Centisome position: 4.78

GC content: 70.41

Gene sequence:

>2352_bases
ATGGTCCGGGTCAAGGTGAACGACCGCATCGTGGAGGTGCCCCCGGGGACGAGCGTCATGGACGCCGTCTTCCACGCGGG
GTACGACGTGCCCCTCTTCTGCTCGGAAAAGCACCTCTCCCCCATAGGGGCCTGCCGCATGTGCCTGGTGCGGATCGGCC
TTCCCAAGAAGGGCCCCGACGGGAAGCCCCTCCTCAACGAGAAGGGCGAGCCTGAGATCCAGTGGCAGCCCAAGCTCGCC
GCAAGCTGCGTGACGGCGGTGGCGGACGGGATGGTGGTGGACACCCTCTCGGACGTGGTGCGGGAGGCCCAGGCGGGGAT
GGTGGAGTTCACCCTCCTCAACCACCCTCTGGACTGCCCTACCTGCGACAAGGGCGGGGCCTGCGAGCTCCAGGACCGCA
CCGTGGAGTACGGGCTTTACGAGAAGTACTACCAGAAGGGCCCCCTGGAGCTTCCCGTCTACACCCGCTTTGAGTTCACC
CGCCGCCACGTGGACAAGCACCACCCCCTCTCCCCCTTCGTCATCCTGGACCGGGAGCGGTGCATCCACTGCAAGCGGTG
CGTGCGCTACTTTGAGGAGGTCCCCGGGGACGAGGTCCTGGACTTCATTGAGCGGGGAGTGCACACCTTCATCGGCACCA
TGGACTTCGGCCTCCCCTCGGGCTTCTCCGGGAACATCACGGACATCTGCCCCGTGGGGGCCCTTCTGGACCTCACCGCC
CGCTTCCGCGCCCGAAACTGGGAGATGGAGGAGACCCCCACCACCTGCGCCCTCTGCCCCGTGGGGTGCGGGATCACCGC
CGACACCCGTAGCGGCGAGCTCCTCAGGATCCGGGCCCGGGAGGTCCCCGAGGTCAACGAGATCTGGATCTGTGACGCGG
GCCGCTTCGGCCACGAGTGGGCGGACCAAAACCGCCTCAAGACCCCCCTGGTGCGGAAAGAGGGAAGGCTCGTGGAGGCC
ACCTGGGAGGAGGCCTTCCTCGCCCTCAAGGAGGGCCTGAAGGAGGCGAGGGGCGAGGAGGTGGGGCTTTACCTCGCCCA
CGACGCCACCCTCGAGGAGGGCCTCTTGGCCTCCGAGCTCGCCAAGGCCCTGAAGACCCCCCACCTGGACTTCCAGGGCC
GCACCGCCGCCCCGGCGAGCCTCTTCCCGCCCGCCTCCCTGGAGGACCTCCTCCAGGCGGACTTCGCCCTGGTCCTCGGC
GACCCCACGGAGGAAGCCCCCATCCTCCACCTCCGCCTCTCCGAGTTCGTGCGCGACCTCAAGCCTCCCCACCGCTACAA
CCACGGCACCCCCTTCGCCGACCTGCAGATCAAGGAAAGGATGCCCCGCCGCACGGACAAGATGGCCCTCTTCGCCCCGT
ACCGCGCCCCCCTCATGAAGTGGGCCGCCATCCACGAGGTCCACCGCCCCGGGGAAGAGCGGGAGATCCTCCTCGCCCTC
CTCGGGGACAAGGAGGGAAGCGAAATGGTGGCGAAGGCGAAGGAGGCCTGGGAGAAGGCGAAGAACCCGGTGCTCATCCT
CGGGGCCGGTGTCCTCCAGGACACCGTGGCCGCGGAAAGGGCCCGCCTCCTCGCCGAGCGCAAGGGGGCCAAGGTCCTCG
CCATGACCCCGGCGGCGAACGCCCGGGGCCTCGAGGCCATGGGGGTCCTCCCCGGGGCGAAGGGGGCCTCCTGGGACGAG
CCCGGGGCCCTCTACGCCTACTACGGCTTCGTGCCCCCGGAAGAGGCCCTTAAGGGCAAGCGCTTCGTGGTGATGCACCT
AAGCCACCTCCACCCCCTGGCGGAGCGCTACGCCCACGTGGTCCTCCCCGCCCCCACCTTCTACGAGAAGCGGGGGCACC
TGGTGAACCTGGAGGGCCGGGTCCTCCCCCTAAGCCCCGCCCCCATAGAGAACGGGGAGGCGGAGGGCGCCCTCCAGGTC
CTCGCCCTCCTGGCCGAGGCCCTGGGGGTGAGGCCCCCCTTCCGGCTCCACCTCGAGGCGCAGAAGGCCCTGAAGGCCCG
GAAGGTCCCGGAGGCCATGGGCCGTCTCTCTTTCCGCCTCAAGGAGCTCCGGCCCAAGGAGCGGAAGGGCGCCTTCTACC
TCCGGCCCACCATGTGGAAGGCCCACCAGGCGGTGGGCAAGGCCCAAGAGGCGGCCCGGGCGGAACTCTGGGCCCACCCG
GAGACGGCGCGGGCCGAGGCCCTGCCCGAGGGGGCCCAGGTGGCCGTGGAGACCCCCTTCGGCCGGGTGGAGGCCCGGGT
GGTCCACCGGGAGGACGTGCCCAAAGGCCACCTCTACCTCTCGGCCCTGGGGCCCGCGGCGGGCCTCCGGGTGGAGGGGC
GCGTCCTGGTGCCCGCGGGAGGTGAAGCGTGA

Upstream 100 bases:

>100_bases
GTGTGGCCGGTGAAGGGCTCGCTAAGGCACTTCAAGGACCAGTACCTGGCCCTGGCGCGGGAGAAGCGCCCCGTGCCCAG
GCCTTCCCTCTGGAGGTGAG

Downstream 100 bases:

>100_bases
CCTGGTCCTACCCCGTAGACCCTTACTGGATGGTGGCCCTGAAGGCCCTCTTGGTGGTGGTGGGCCTCCTCACCGCCTTC
GCCTTCATGACCCTCATTGA

Product: NADH-quinone oxidoreductase subunit 3

Products: NA

Alternate protein names: NADH dehydrogenase I chain 3; NDH-1 subunit 3

Number of amino acids: Translated: 783; Mature: 783

Protein sequence:

>783_residues
MVRVKVNDRIVEVPPGTSVMDAVFHAGYDVPLFCSEKHLSPIGACRMCLVRIGLPKKGPDGKPLLNEKGEPEIQWQPKLA
ASCVTAVADGMVVDTLSDVVREAQAGMVEFTLLNHPLDCPTCDKGGACELQDRTVEYGLYEKYYQKGPLELPVYTRFEFT
RRHVDKHHPLSPFVILDRERCIHCKRCVRYFEEVPGDEVLDFIERGVHTFIGTMDFGLPSGFSGNITDICPVGALLDLTA
RFRARNWEMEETPTTCALCPVGCGITADTRSGELLRIRAREVPEVNEIWICDAGRFGHEWADQNRLKTPLVRKEGRLVEA
TWEEAFLALKEGLKEARGEEVGLYLAHDATLEEGLLASELAKALKTPHLDFQGRTAAPASLFPPASLEDLLQADFALVLG
DPTEEAPILHLRLSEFVRDLKPPHRYNHGTPFADLQIKERMPRRTDKMALFAPYRAPLMKWAAIHEVHRPGEEREILLAL
LGDKEGSEMVAKAKEAWEKAKNPVLILGAGVLQDTVAAERARLLAERKGAKVLAMTPAANARGLEAMGVLPGAKGASWDE
PGALYAYYGFVPPEEALKGKRFVVMHLSHLHPLAERYAHVVLPAPTFYEKRGHLVNLEGRVLPLSPAPIENGEAEGALQV
LALLAEALGVRPPFRLHLEAQKALKARKVPEAMGRLSFRLKELRPKERKGAFYLRPTMWKAHQAVGKAQEAARAELWAHP
ETARAEALPEGAQVAVETPFGRVEARVVHREDVPKGHLYLSALGPAAGLRVEGRVLVPAGGEA

Sequences:

>Translated_783_residues
MVRVKVNDRIVEVPPGTSVMDAVFHAGYDVPLFCSEKHLSPIGACRMCLVRIGLPKKGPDGKPLLNEKGEPEIQWQPKLA
ASCVTAVADGMVVDTLSDVVREAQAGMVEFTLLNHPLDCPTCDKGGACELQDRTVEYGLYEKYYQKGPLELPVYTRFEFT
RRHVDKHHPLSPFVILDRERCIHCKRCVRYFEEVPGDEVLDFIERGVHTFIGTMDFGLPSGFSGNITDICPVGALLDLTA
RFRARNWEMEETPTTCALCPVGCGITADTRSGELLRIRAREVPEVNEIWICDAGRFGHEWADQNRLKTPLVRKEGRLVEA
TWEEAFLALKEGLKEARGEEVGLYLAHDATLEEGLLASELAKALKTPHLDFQGRTAAPASLFPPASLEDLLQADFALVLG
DPTEEAPILHLRLSEFVRDLKPPHRYNHGTPFADLQIKERMPRRTDKMALFAPYRAPLMKWAAIHEVHRPGEEREILLAL
LGDKEGSEMVAKAKEAWEKAKNPVLILGAGVLQDTVAAERARLLAERKGAKVLAMTPAANARGLEAMGVLPGAKGASWDE
PGALYAYYGFVPPEEALKGKRFVVMHLSHLHPLAERYAHVVLPAPTFYEKRGHLVNLEGRVLPLSPAPIENGEAEGALQV
LALLAEALGVRPPFRLHLEAQKALKARKVPEAMGRLSFRLKELRPKERKGAFYLRPTMWKAHQAVGKAQEAARAELWAHP
ETARAEALPEGAQVAVETPFGRVEARVVHREDVPKGHLYLSALGPAAGLRVEGRVLVPAGGEA
>Mature_783_residues
MVRVKVNDRIVEVPPGTSVMDAVFHAGYDVPLFCSEKHLSPIGACRMCLVRIGLPKKGPDGKPLLNEKGEPEIQWQPKLA
ASCVTAVADGMVVDTLSDVVREAQAGMVEFTLLNHPLDCPTCDKGGACELQDRTVEYGLYEKYYQKGPLELPVYTRFEFT
RRHVDKHHPLSPFVILDRERCIHCKRCVRYFEEVPGDEVLDFIERGVHTFIGTMDFGLPSGFSGNITDICPVGALLDLTA
RFRARNWEMEETPTTCALCPVGCGITADTRSGELLRIRAREVPEVNEIWICDAGRFGHEWADQNRLKTPLVRKEGRLVEA
TWEEAFLALKEGLKEARGEEVGLYLAHDATLEEGLLASELAKALKTPHLDFQGRTAAPASLFPPASLEDLLQADFALVLG
DPTEEAPILHLRLSEFVRDLKPPHRYNHGTPFADLQIKERMPRRTDKMALFAPYRAPLMKWAAIHEVHRPGEEREILLAL
LGDKEGSEMVAKAKEAWEKAKNPVLILGAGVLQDTVAAERARLLAERKGAKVLAMTPAANARGLEAMGVLPGAKGASWDE
PGALYAYYGFVPPEEALKGKRFVVMHLSHLHPLAERYAHVVLPAPTFYEKRGHLVNLEGRVLPLSPAPIENGEAEGALQV
LALLAEALGVRPPFRLHLEAQKALKARKVPEAMGRLSFRLKELRPKERKGAFYLRPTMWKAHQAVGKAQEAARAELWAHP
ETARAEALPEGAQVAVETPFGRVEARVVHREDVPKGHLYLSALGPAAGLRVEGRVLVPAGGEA

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every

COG id: COG1034

COG function: function code C; NADH dehydrogenase/NADH:ubiquinone oxidoreductase 75 kD subunit (chain G)

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 2Fe-2S ferredoxin-type domain

Homologues:

Organism=Homo sapiens, GI33519475, Length=674, Percent_Identity=28.486646884273, Blast_Score=229, Evalue=7e-60,
Organism=Escherichia coli, GI145693161, Length=677, Percent_Identity=26.7355982274741, Blast_Score=200, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI17565758, Length=685, Percent_Identity=29.7810218978102, Blast_Score=232, Evalue=6e-61,
Organism=Caenorhabditis elegans, GI32566231, Length=651, Percent_Identity=30.2611367127496, Blast_Score=226, Evalue=4e-59,
Organism=Caenorhabditis elegans, GI193209088, Length=288, Percent_Identity=37.8472222222222, Blast_Score=179, Evalue=7e-45,
Organism=Drosophila melanogaster, GI24640559, Length=756, Percent_Identity=30.4232804232804, Blast_Score=243, Evalue=3e-64,
Organism=Drosophila melanogaster, GI24640557, Length=756, Percent_Identity=30.4232804232804, Blast_Score=243, Evalue=3e-64,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NQO3_THET8 (Q56223)

Other databases:

- EMBL:   U52917
- EMBL:   AP008226
- PIR:   T11904
- RefSeq:   YP_143356.1
- PDB:   2FUG
- PDB:   3I9V
- PDB:   3IAM
- PDB:   3IAS
- PDB:   3M9S
- PDBsum:   2FUG
- PDBsum:   3I9V
- PDBsum:   3IAM
- PDBsum:   3IAS
- PDBsum:   3M9S
- ProteinModelPortal:   Q56223
- SMR:   Q56223
- STRING:   Q56223
- GeneID:   3168388
- GenomeReviews:   AP008226_GR
- KEGG:   ttj:TTHA0090
- NMPDR:   fig|300852.3.peg.121
- eggNOG:   COG1034
- HOGENOM:   HBG715033
- OMA:   CVRFAND
- ProtClustDB:   CLSK497043
- BioCyc:   TTHE300852:TTHA0090-MONOMER
- InterPro:   IPR009010
- InterPro:   IPR012675
- InterPro:   IPR001041
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR000283
- InterPro:   IPR010228
- InterPro:   IPR019574
- Gene3D:   G3DSA:3.10.20.30
- SMART:   SM00926
- SMART:   SM00929
- TIGRFAMs:   TIGR01973

Pfam domain/function: PF00111 Fer2; PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding; PF10588 NADH-G_4Fe-4S_3; SSF50692 Asp_decarb_fold; SSF54292 Ferredoxin

EC number: =1.6.99.5

Molecular weight: Translated: 86530; Mature: 86530

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS00197 2FE2S_FER_1; PS51085 2FE2S_FER_2; PS00641 COMPLEX1_75K_1; PS00642 COMPLEX1_75K_2; PS00643 COMPLEX1_75K_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRVKVNDRIVEVPPGTSVMDAVFHAGYDVPLFCSEKHLSPIGACRMCLVRIGLPKKGPD
CEEEEECCEEEECCCCCHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCC
GKPLLNEKGEPEIQWQPKLAASCVTAVADGMVVDTLSDVVREAQAGMVEFTLLNHPLDCP
CCCCCCCCCCCCEEECCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCC
TCDKGGACELQDRTVEYGLYEKYYQKGPLELPVYTRFEFTRRHVDKHHPLSPFVILDRER
CCCCCCCCEECCCHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHCCCCCCCEEEECHHH
CIHCKRCVRYFEEVPGDEVLDFIERGVHTFIGTMDFGLPSGFSGNITDICPVGALLDLTA
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHH
RFRARNWEMEETPTTCALCPVGCGITADTRSGELLRIRAREVPEVNEIWICDAGRFGHEW
HHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCEEEEECCCCCCCHH
ADQNRLKTPLVRKEGRLVEATWEEAFLALKEGLKEARGEEVGLYLAHDATLEEGLLASEL
HCCCCCCCCHHHCCCCEEEHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHH
AKALKTPHLDFQGRTAAPASLFPPASLEDLLQADFALVLGDPTEEAPILHLRLSEFVRDL
HHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCEEEEECCCCCCCCEEEEEHHHHHHHC
KPPHRYNHGTPFADLQIKERMPRRTDKMALFAPYRAPLMKWAAIHEVHRPGEEREILLAL
CCCCCCCCCCCCCCEEHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCEEEEEE
LGDKEGSEMVAKAKEAWEKAKNPVLILGAGVLQDTVAAERARLLAERKGAKVLAMTPAAN
ECCCCCHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHCCCEEEEECCCCC
ARGLEAMGVLPGAKGASWDEPGALYAYYGFVPPEEALKGKRFVVMHLSHLHPLAERYAHV
CCCCEEEECCCCCCCCCCCCCCCEEEEECCCCHHHHHCCCEEEEEEHHHHHHHHHHCEEE
VLPAPTFYEKRGHLVNLEGRVLPLSPAPIENGEAEGALQVLALLAEALGVRPPFRLHLEA
EECCCHHHHCCCCEEECCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEHH
QKALKARKVPEAMGRLSFRLKELRPKERKGAFYLRPTMWKAHQAVGKAQEAARAELWAHP
HHHHHHHCCHHHHHHHHHHHHHCCCHHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCC
ETARAEALPEGAQVAVETPFGRVEARVVHREDVPKGHLYLSALGPAAGLRVEGRVLVPAG
CHHHHHCCCCCCEEEEECCCCCEEEEEEECCCCCCCEEEEEECCCCCCCEECCEEEECCC
GEA
CCC
>Mature Secondary Structure
MVRVKVNDRIVEVPPGTSVMDAVFHAGYDVPLFCSEKHLSPIGACRMCLVRIGLPKKGPD
CEEEEECCEEEECCCCCHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCC
GKPLLNEKGEPEIQWQPKLAASCVTAVADGMVVDTLSDVVREAQAGMVEFTLLNHPLDCP
CCCCCCCCCCCCEEECCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCC
TCDKGGACELQDRTVEYGLYEKYYQKGPLELPVYTRFEFTRRHVDKHHPLSPFVILDRER
CCCCCCCCEECCCHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHCCCCCCCEEEECHHH
CIHCKRCVRYFEEVPGDEVLDFIERGVHTFIGTMDFGLPSGFSGNITDICPVGALLDLTA
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHH
RFRARNWEMEETPTTCALCPVGCGITADTRSGELLRIRAREVPEVNEIWICDAGRFGHEW
HHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCEEEEECCCCCCCHH
ADQNRLKTPLVRKEGRLVEATWEEAFLALKEGLKEARGEEVGLYLAHDATLEEGLLASEL
HCCCCCCCCHHHCCCCEEEHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHH
AKALKTPHLDFQGRTAAPASLFPPASLEDLLQADFALVLGDPTEEAPILHLRLSEFVRDL
HHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCEEEEECCCCCCCCEEEEEHHHHHHHC
KPPHRYNHGTPFADLQIKERMPRRTDKMALFAPYRAPLMKWAAIHEVHRPGEEREILLAL
CCCCCCCCCCCCCCEEHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCEEEEEE
LGDKEGSEMVAKAKEAWEKAKNPVLILGAGVLQDTVAAERARLLAERKGAKVLAMTPAAN
ECCCCCHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHCCCEEEEECCCCC
ARGLEAMGVLPGAKGASWDEPGALYAYYGFVPPEEALKGKRFVVMHLSHLHPLAERYAHV
CCCCEEEECCCCCCCCCCCCCCCEEEEECCCCHHHHHCCCEEEEEEHHHHHHHHHHCEEE
VLPAPTFYEKRGHLVNLEGRVLPLSPAPIENGEAEGALQVLALLAEALGVRPPFRLHLEA
EECCCHHHHCCCCEEECCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEHH
QKALKARKVPEAMGRLSFRLKELRPKERKGAFYLRPTMWKAHQAVGKAQEAARAELWAHP
HHHHHHHCCHHHHHHHHHHHHHCCCHHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCC
ETARAEALPEGAQVAVETPFGRVEARVVHREDVPKGHLYLSALGPAAGLRVEGRVLVPAG
CHHHHHCCCCCCEEEEECCCCCEEEEEEECCCCCCCEEEEEECCCCCCCEECCEEEECCC
GEA
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9020134