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The map label for this gene is eno

Identifier: 55979971

GI number: 55979971

Start: 1235

End: 2503

Strand: Direct

Name: eno

Synonym: TTHA0002

Alternate gene names: 55979971

Gene position: 1235-2503 (Clockwise)

Preceding gene: 55979970

Following gene: 55979972

Centisome position: 0.07

GC content: 69.27

Gene sequence:

>1269_bases
ATGACCACCATCGTCGGCGTCCGGGCACGCGAGGTTTTGGATTCCAGGGGCTTTCCCACGGTAGAGGCGGAGGTGGAGCT
GGAAGGCGGGGCCAGGGGCCGGGCCATGGTGCCCTCCGGGGCCTCCACCGGAACCCACGAGGCCCTGGAGCTCAGGGACG
GCGGCAAGCGCTACCTGGGCAAGGGGGTGCGCCGGGCGGTGGAGAACGTCAACGAGCGCATCGCCCCCGAGCTCGTCGGC
ATGGACGCCCTGGACCAGGAAGGGGTGGACCGGGCCATGCTGGAGCTGGACGGCACCCCCAACAAGGCCAACCTGGGGGC
GAACGCCGTCCTCGCGGTCTCCCTGGCCGTGGCCCGGGCGGCGGCCGAGGCCCTGGGCCTGCCCCTTTACCGCTACCTGG
GCGGGGTCCAGGGGGTCACCCTGCCCGTGCCCCTCATGAACGTCATCAACGGGGGGAAGCACGCCGACAACCGGGTGGAC
TTCCAGGAGTTCATGCTGGTGCCCGCGGGGGCGGGAAGCTTCGCCGAGGCCTTGAGGATCGGGGCCGAGGTCTTCCACAC
CCTCAAGGCCGTCCTCAAGGAGAAGGGCTACAGCACCAACGTGGGGGACGAGGGGGGCTTCGCCCCCGACCTCAGGAGCA
ACGAGGAGGCGGTGGAGCTTTTGCTCCTCGCCATTGAGCGGGCGGGGTACACCCCGGGCCAGGAGGTCTCCCTGGCCCTG
GACCCGGCCACGAGCGAGCTTTACCGGGACGGGAAGTACCACCTGGAGGGGGAGGGCAAGGTCCTCTCCTCGGAGGAGAT
GGTGGCCTTCTGGGAGGCCTGGGTGGAGAAGTACCCCATCCGCTCCATTGAGGACGGCCTCGCCGAGGACGACTGGGAGG
GGTGGCGGCTTCTCACCGAGCGCCTGGGGGGGAAGGTCCAGCTCGTGGGGGACGACCTCTTCGTCACCAACCCGGAAAGG
CTCCGGGCGGGGATTGAGCGGGGGGTGGCCAACGCCATCCTGGTCAAGGTGAACCAGATCGGGACCCTCTCGGAGACCCT
CGAGGCCATCCGCCTGGCCCAGCGCTCGGGGTACAGGGCGGTGATCAGCCACCGCTCCGGGGAGACGGAGGACAGCTTCA
TCGCCGACCTCGCCGTGGCGGTGAACGCCGGACAGATCAAGACCGGTTCCCTTTCCCGCTCCGACCGGCTGGCCAAGTAC
AACCAGCTCCTGCGCATTGAGGAGGAGCTGGGCCGGGCCGCGAGGTTTTTGGGGTATGCCGCCTTTTAA

Upstream 100 bases:

>100_bases
GGGGACGGGGAGGGGTACCGGGCGGTGGTGGTGCCCCTCAGGGTCTAGGGGGTAGTATGGGGTGGGGGAGCGTGAAGCGC
TTCCACAAGGGAGGTTGGGC

Downstream 100 bases:

>100_bases
GCGCACCAAGATCGTGGCCACCCTGGGGCCGGCCACGGACGACAAGGAGGTGATCCGCGCCCTGGCGGAGGCCGGGGCCG
ACGTCTTCCGCCTGAACTTC

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 422; Mature: 421

Protein sequence:

>422_residues
MTTIVGVRAREVLDSRGFPTVEAEVELEGGARGRAMVPSGASTGTHEALELRDGGKRYLGKGVRRAVENVNERIAPELVG
MDALDQEGVDRAMLELDGTPNKANLGANAVLAVSLAVARAAAEALGLPLYRYLGGVQGVTLPVPLMNVINGGKHADNRVD
FQEFMLVPAGAGSFAEALRIGAEVFHTLKAVLKEKGYSTNVGDEGGFAPDLRSNEEAVELLLLAIERAGYTPGQEVSLAL
DPATSELYRDGKYHLEGEGKVLSSEEMVAFWEAWVEKYPIRSIEDGLAEDDWEGWRLLTERLGGKVQLVGDDLFVTNPER
LRAGIERGVANAILVKVNQIGTLSETLEAIRLAQRSGYRAVISHRSGETEDSFIADLAVAVNAGQIKTGSLSRSDRLAKY
NQLLRIEEELGRAARFLGYAAF

Sequences:

>Translated_422_residues
MTTIVGVRAREVLDSRGFPTVEAEVELEGGARGRAMVPSGASTGTHEALELRDGGKRYLGKGVRRAVENVNERIAPELVG
MDALDQEGVDRAMLELDGTPNKANLGANAVLAVSLAVARAAAEALGLPLYRYLGGVQGVTLPVPLMNVINGGKHADNRVD
FQEFMLVPAGAGSFAEALRIGAEVFHTLKAVLKEKGYSTNVGDEGGFAPDLRSNEEAVELLLLAIERAGYTPGQEVSLAL
DPATSELYRDGKYHLEGEGKVLSSEEMVAFWEAWVEKYPIRSIEDGLAEDDWEGWRLLTERLGGKVQLVGDDLFVTNPER
LRAGIERGVANAILVKVNQIGTLSETLEAIRLAQRSGYRAVISHRSGETEDSFIADLAVAVNAGQIKTGSLSRSDRLAKY
NQLLRIEEELGRAARFLGYAAF
>Mature_421_residues
TTIVGVRAREVLDSRGFPTVEAEVELEGGARGRAMVPSGASTGTHEALELRDGGKRYLGKGVRRAVENVNERIAPELVGM
DALDQEGVDRAMLELDGTPNKANLGANAVLAVSLAVARAAAEALGLPLYRYLGGVQGVTLPVPLMNVINGGKHADNRVDF
QEFMLVPAGAGSFAEALRIGAEVFHTLKAVLKEKGYSTNVGDEGGFAPDLRSNEEAVELLLLAIERAGYTPGQEVSLALD
PATSELYRDGKYHLEGEGKVLSSEEMVAFWEAWVEKYPIRSIEDGLAEDDWEGWRLLTERLGGKVQLVGDDLFVTNPERL
RAGIERGVANAILVKVNQIGTLSETLEAIRLAQRSGYRAVISHRSGETEDSFIADLAVAVNAGQIKTGSLSRSDRLAKYN
QLLRIEEELGRAARFLGYAAF

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=430, Percent_Identity=53.2558139534884, Blast_Score=441, Evalue=1e-124,
Organism=Homo sapiens, GI4503571, Length=429, Percent_Identity=52.2144522144522, Blast_Score=439, Evalue=1e-123,
Organism=Homo sapiens, GI301897477, Length=426, Percent_Identity=53.2863849765258, Blast_Score=434, Evalue=1e-122,
Organism=Homo sapiens, GI301897469, Length=426, Percent_Identity=53.2863849765258, Blast_Score=434, Evalue=1e-122,
Organism=Homo sapiens, GI301897479, Length=424, Percent_Identity=48.5849056603774, Blast_Score=374, Evalue=1e-104,
Organism=Homo sapiens, GI169201331, Length=336, Percent_Identity=27.6785714285714, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI169201757, Length=336, Percent_Identity=27.6785714285714, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI239744207, Length=336, Percent_Identity=27.6785714285714, Blast_Score=100, Evalue=3e-21,
Organism=Escherichia coli, GI1789141, Length=424, Percent_Identity=60.6132075471698, Blast_Score=500, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI71995829, Length=431, Percent_Identity=52.6682134570766, Blast_Score=439, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI17536383, Length=431, Percent_Identity=52.6682134570766, Blast_Score=439, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI32563855, Length=191, Percent_Identity=47.6439790575916, Blast_Score=194, Evalue=6e-50,
Organism=Saccharomyces cerevisiae, GI6321693, Length=428, Percent_Identity=51.1682242990654, Blast_Score=402, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6323985, Length=433, Percent_Identity=49.8845265588915, Blast_Score=397, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6324974, Length=433, Percent_Identity=49.6535796766744, Blast_Score=395, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6324969, Length=433, Percent_Identity=49.6535796766744, Blast_Score=395, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6321968, Length=428, Percent_Identity=50.7009345794392, Blast_Score=372, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24580918, Length=430, Percent_Identity=49.7674418604651, Blast_Score=407, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580916, Length=430, Percent_Identity=49.7674418604651, Blast_Score=407, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580920, Length=430, Percent_Identity=49.7674418604651, Blast_Score=407, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580914, Length=430, Percent_Identity=49.7674418604651, Blast_Score=407, Evalue=1e-114,
Organism=Drosophila melanogaster, GI281360527, Length=430, Percent_Identity=49.7674418604651, Blast_Score=407, Evalue=1e-114,
Organism=Drosophila melanogaster, GI17137654, Length=430, Percent_Identity=49.7674418604651, Blast_Score=407, Evalue=1e-114,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_THET2 (Q72H85)

Other databases:

- EMBL:   AE017221
- RefSeq:   YP_005579.1
- ProteinModelPortal:   Q72H85
- SMR:   Q72H85
- STRING:   Q72H85
- GeneID:   2775102
- GenomeReviews:   AE017221_GR
- eggNOG:   COG0148
- HOGENOM:   HBG726599
- OMA:   GSHADSN
- PhylomeDB:   Q72H85
- ProtClustDB:   PRK00077
- BioCyc:   TTHE262724:TT_C1610-MONOMER
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 45501; Mature: 45370

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 204-204 ACT_SITE 336-336 BINDING 154-154 BINDING 163-163 BINDING 284-284 BINDING 311-311 BINDING 336-336 BINDING 387-387

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTIVGVRAREVLDSRGFPTVEAEVELEGGARGRAMVPSGASTGTHEALELRDGGKRYLG
CCCEECHHHHHHHHCCCCCCEEEEEEECCCCCCCEECCCCCCCCCCHHHEECCCCHHHHH
KGVRRAVENVNERIAPELVGMDALDQEGVDRAMLELDGTPNKANLGANAVLAVSLAVARA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHEECCCCCCCCCCCHHHHHHHHHHHHH
AAEALGLPLYRYLGGVQGVTLPVPLMNVINGGKHADNRVDFQEFMLVPAGAGSFAEALRI
HHHHHCCHHHHHHCCCCCCEECHHHHHHHCCCCCCCCCCCHHHHEEECCCCCHHHHHHHH
GAEVFHTLKAVLKEKGYSTNVGDEGGFAPDLRSNEEAVELLLLAIERAGYTPGQEVSLAL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEE
DPATSELYRDGKYHLEGEGKVLSSEEMVAFWEAWVEKYPIRSIEDGLAEDDWEGWRLLTE
CCCHHHHHCCCCEEECCCCCEECCHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHH
RLGGKVQLVGDDLFVTNPERLRAGIERGVANAILVKVNQIGTLSETLEAIRLAQRSGYRA
HHCCEEEEEECCEEEECHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCEE
VISHRSGETEDSFIADLAVAVNAGQIKTGSLSRSDRLAKYNQLLRIEEELGRAARFLGYA
EEECCCCCCCHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AF
CC
>Mature Secondary Structure 
TTIVGVRAREVLDSRGFPTVEAEVELEGGARGRAMVPSGASTGTHEALELRDGGKRYLG
CCEECHHHHHHHHCCCCCCEEEEEEECCCCCCCEECCCCCCCCCCHHHEECCCCHHHHH
KGVRRAVENVNERIAPELVGMDALDQEGVDRAMLELDGTPNKANLGANAVLAVSLAVARA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHEECCCCCCCCCCCHHHHHHHHHHHHH
AAEALGLPLYRYLGGVQGVTLPVPLMNVINGGKHADNRVDFQEFMLVPAGAGSFAEALRI
HHHHHCCHHHHHHCCCCCCEECHHHHHHHCCCCCCCCCCCHHHHEEECCCCCHHHHHHHH
GAEVFHTLKAVLKEKGYSTNVGDEGGFAPDLRSNEEAVELLLLAIERAGYTPGQEVSLAL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEE
DPATSELYRDGKYHLEGEGKVLSSEEMVAFWEAWVEKYPIRSIEDGLAEDDWEGWRLLTE
CCCHHHHHCCCCEEECCCCCEECCHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHH
RLGGKVQLVGDDLFVTNPERLRAGIERGVANAILVKVNQIGTLSETLEAIRLAQRSGYRA
HHCCEEEEEECCEEEECHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCEE
VISHRSGETEDSFIADLAVAVNAGQIKTGSLSRSDRLAKYNQLLRIEEELGRAARFLGYA
EEECCCCCCCHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AF
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA