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The map label for this gene is pyk [H]

Identifier: 55979972

GI number: 55979972

Start: 2490

End: 3914

Strand: Direct

Name: pyk [H]

Synonym: TTHA0003

Alternate gene names: 55979972

Gene position: 2490-3914 (Clockwise)

Preceding gene: 55979971

Following gene: 55979978

Centisome position: 0.13

GC content: 71.02

Gene sequence:

>1425_bases
ATGCCGCCTTTTAAGCGCACCAAGATCGTGGCCACCCTGGGGCCGGCCACGGACGACAAGGAGGTGATCCGCGCCCTGGC
GGAGGCCGGGGCCGACGTCTTCCGCCTGAACTTCAGCCACGGCGCCCCCGAGGACCACAGGCGCCGGGTGGGCTGGGTGC
GGGAGGTGGCGGAGGAGCTCGGCCGAACCCTGGCCGTCCTCCAGGACCTCCAGGGCCCGAAGATCCGTGTGGGCCGTTTC
CGGGAGGGCCAGGTTCTCCTCCGCCCGGGGCAGAGGTTCGTCCTCACCGCCGAGCCCGTGGAGGGGGACGAGCACCGCGT
TTCCGTGAGCTACAAGGGGCTTCCCGAGGACGTTTCCCCGGGGCAGATCCTCCTTCTGGACGACGGCCGCATCCGGCTCA
AGGTCCTGGAGGTCCGGAGCCCGGAGATCCTCACGGAGGTGGAGGTGGGCGGGGTCCTCTCCAACAACAAGGGGATCAAC
ATCCCTGGGGCGGACCTCTCCATCCCCGCCCTCTCGGAGAAGGATATCCAGGACCTGGCCCTGGGGGCGGAGCTCGGGGT
GGACTGGGTGGCGGTCTCCTTCGTCCGCACGCGGGACGACCTCCTCCTCGCCCGGCACTACCTCTCCCGCTACGGCTCCA
AGGCCAGGCTCATGGCCAAGATCGAGAAGCCCTCGGCCGTGGCCCGGTTTGAGGAGATCCTGGAGGAGGCGGACGGGATC
ATGGTGGCCCGGGGGGACCTCGGGGTGGAGATGCCCCTGGAGGAGGTGCCCATCGTCCAGAAGCGCCTCATCCTCCGGTG
CATCGCCGCCGGGAAGCCGGTGATCACCGCCACCCAGATGCTGGAGTCCATGGTGCAGAACCCGAGCCCCACCCGGGCCG
AGGCCTCGGACGTGGCCAACGCCATCTTTGACGGGACCGACGCGGTGATGCTCTCCGCGGAGACGGCGGCCGGGGCCTAC
CCGGTGGAGGCGGTGGCCATGATGGCGAGGATCGCCAAGGCGGTGGAGTCTTCCCCGGAGTTCTTGCAGAAGCTCAACGT
CCTCCGCCCCGCCCCCACCCCCACCACCCAGGACGCCATCGCCCAGGCGGCGGACGACGTGGTGGAGGCGGTGGGGGCCC
GGGCCATCGTGGTCTTCACGGCCACGGGCGGCTCGGCGCGGAGGATCGCCCGCACCCGGCCCCAGGTGCCCATCCTGGCC
CTCACCCCGAACCCCGAGGTGCGGAACCAGCTGGCCTTGGTCTGGGGGGTCTACCCCCATCTCGCCCCCGACCCCCAGGA
CACCGACGACATGGTCCGCATCGCCCTCAGGGAGGTGAAGGCCCTGGGGCTCGCCCAGGTGGGGGACCGGGTGGTCATCG
CCGCCGGGGTTCCCTTCGGGGTCCGGGGGACGACGAACCTCATCCGCGTGGAGCGGGTGGGGTAG

Upstream 100 bases:

>100_bases
ATCAAGACCGGTTCCCTTTCCCGCTCCGACCGGCTGGCCAAGTACAACCAGCTCCTGCGCATTGAGGAGGAGCTGGGCCG
GGCCGCGAGGTTTTTGGGGT

Downstream 100 bases:

>100_bases
CTACCGGGGCAGGGTGAGCCGGTAGCGGAAAGAGCGGGCCTCCATGGGGTCCAGGACCGCCTCGAGGCGGTACCCCTCCG
GCAGGAGGACCGCGCCGGGG

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MPPFKRTKIVATLGPATDDKEVIRALAEAGADVFRLNFSHGAPEDHRRRVGWVREVAEELGRTLAVLQDLQGPKIRVGRF
REGQVLLRPGQRFVLTAEPVEGDEHRVSVSYKGLPEDVSPGQILLLDDGRIRLKVLEVRSPEILTEVEVGGVLSNNKGIN
IPGADLSIPALSEKDIQDLALGAELGVDWVAVSFVRTRDDLLLARHYLSRYGSKARLMAKIEKPSAVARFEEILEEADGI
MVARGDLGVEMPLEEVPIVQKRLILRCIAAGKPVITATQMLESMVQNPSPTRAEASDVANAIFDGTDAVMLSAETAAGAY
PVEAVAMMARIAKAVESSPEFLQKLNVLRPAPTPTTQDAIAQAADDVVEAVGARAIVVFTATGGSARRIARTRPQVPILA
LTPNPEVRNQLALVWGVYPHLAPDPQDTDDMVRIALREVKALGLAQVGDRVVIAAGVPFGVRGTTNLIRVERVG

Sequences:

>Translated_474_residues
MPPFKRTKIVATLGPATDDKEVIRALAEAGADVFRLNFSHGAPEDHRRRVGWVREVAEELGRTLAVLQDLQGPKIRVGRF
REGQVLLRPGQRFVLTAEPVEGDEHRVSVSYKGLPEDVSPGQILLLDDGRIRLKVLEVRSPEILTEVEVGGVLSNNKGIN
IPGADLSIPALSEKDIQDLALGAELGVDWVAVSFVRTRDDLLLARHYLSRYGSKARLMAKIEKPSAVARFEEILEEADGI
MVARGDLGVEMPLEEVPIVQKRLILRCIAAGKPVITATQMLESMVQNPSPTRAEASDVANAIFDGTDAVMLSAETAAGAY
PVEAVAMMARIAKAVESSPEFLQKLNVLRPAPTPTTQDAIAQAADDVVEAVGARAIVVFTATGGSARRIARTRPQVPILA
LTPNPEVRNQLALVWGVYPHLAPDPQDTDDMVRIALREVKALGLAQVGDRVVIAAGVPFGVRGTTNLIRVERVG
>Mature_473_residues
PPFKRTKIVATLGPATDDKEVIRALAEAGADVFRLNFSHGAPEDHRRRVGWVREVAEELGRTLAVLQDLQGPKIRVGRFR
EGQVLLRPGQRFVLTAEPVEGDEHRVSVSYKGLPEDVSPGQILLLDDGRIRLKVLEVRSPEILTEVEVGGVLSNNKGINI
PGADLSIPALSEKDIQDLALGAELGVDWVAVSFVRTRDDLLLARHYLSRYGSKARLMAKIEKPSAVARFEEILEEADGIM
VARGDLGVEMPLEEVPIVQKRLILRCIAAGKPVITATQMLESMVQNPSPTRAEASDVANAIFDGTDAVMLSAETAAGAYP
VEAVAMMARIAKAVESSPEFLQKLNVLRPAPTPTTQDAIAQAADDVVEAVGARAIVVFTATGGSARRIARTRPQVPILAL
TPNPEVRNQLALVWGVYPHLAPDPQDTDDMVRIALREVKALGLAQVGDRVVIAAGVPFGVRGTTNLIRVERVG

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286418, Length=492, Percent_Identity=42.8861788617886, Blast_Score=350, Evalue=1e-96,
Organism=Homo sapiens, GI33286422, Length=492, Percent_Identity=42.0731707317073, Blast_Score=345, Evalue=7e-95,
Organism=Homo sapiens, GI33286420, Length=492, Percent_Identity=42.0731707317073, Blast_Score=345, Evalue=7e-95,
Organism=Homo sapiens, GI32967597, Length=487, Percent_Identity=41.8891170431211, Blast_Score=337, Evalue=1e-92,
Organism=Homo sapiens, GI10835121, Length=487, Percent_Identity=41.8891170431211, Blast_Score=337, Evalue=1e-92,
Organism=Homo sapiens, GI310128732, Length=292, Percent_Identity=41.7808219178082, Blast_Score=207, Evalue=1e-53,
Organism=Homo sapiens, GI310128730, Length=292, Percent_Identity=41.7808219178082, Blast_Score=207, Evalue=1e-53,
Organism=Homo sapiens, GI310128736, Length=242, Percent_Identity=36.7768595041322, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI310128734, Length=242, Percent_Identity=36.7768595041322, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI310128738, Length=214, Percent_Identity=36.9158878504673, Blast_Score=128, Evalue=1e-29,
Organism=Escherichia coli, GI1787965, Length=477, Percent_Identity=44.8637316561845, Blast_Score=382, Evalue=1e-107,
Organism=Escherichia coli, GI1788160, Length=476, Percent_Identity=46.6386554621849, Blast_Score=382, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI17544584, Length=480, Percent_Identity=39.1666666666667, Blast_Score=335, Evalue=5e-92,
Organism=Caenorhabditis elegans, GI17506829, Length=487, Percent_Identity=40.2464065708419, Blast_Score=300, Evalue=9e-82,
Organism=Caenorhabditis elegans, GI71984413, Length=487, Percent_Identity=40.2464065708419, Blast_Score=300, Evalue=1e-81,
Organism=Caenorhabditis elegans, GI71984406, Length=487, Percent_Identity=40.2464065708419, Blast_Score=300, Evalue=1e-81,
Organism=Caenorhabditis elegans, GI17506831, Length=487, Percent_Identity=40.2464065708419, Blast_Score=300, Evalue=2e-81,
Organism=Saccharomyces cerevisiae, GI6319279, Length=486, Percent_Identity=40.1234567901235, Blast_Score=325, Evalue=1e-89,
Organism=Saccharomyces cerevisiae, GI6324923, Length=487, Percent_Identity=39.4250513347023, Blast_Score=316, Evalue=4e-87,
Organism=Drosophila melanogaster, GI24648964, Length=489, Percent_Identity=39.6728016359918, Blast_Score=315, Evalue=5e-86,
Organism=Drosophila melanogaster, GI28571814, Length=489, Percent_Identity=39.6728016359918, Blast_Score=315, Evalue=5e-86,
Organism=Drosophila melanogaster, GI24648966, Length=405, Percent_Identity=42.7160493827161, Blast_Score=305, Evalue=7e-83,
Organism=Drosophila melanogaster, GI24581235, Length=479, Percent_Identity=34.446764091858, Blast_Score=261, Evalue=7e-70,
Organism=Drosophila melanogaster, GI24646914, Length=268, Percent_Identity=41.7910447761194, Blast_Score=188, Evalue=8e-48,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51180; Mature: 51049

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPFKRTKIVATLGPATDDKEVIRALAEAGADVFRLNFSHGAPEDHRRRVGWVREVAEEL
CCCCCCEEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHH
GRTLAVLQDLQGPKIRVGRFREGQVLLRPGQRFVLTAEPVEGDEHRVSVSYKGLPEDVSP
HHHHHHHHHCCCCCEEECCCCCCCEEEECCCEEEEEECCCCCCCEEEEEEECCCCCCCCC
GQILLLDDGRIRLKVLEVRSPEILTEVEVGGVLSNNKGINIPGADLSIPALSEKDIQDLA
CEEEEEECCCEEEEEEEECCCCCEEEEEECCEEECCCCCCCCCCCCCCCCCCCCCHHHHH
LGAELGVDWVAVSFVRTRDDLLLARHYLSRYGSKARLMAKIEKPSAVARFEEILEEADGI
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHCCCE
MVARGDLGVEMPLEEVPIVQKRLILRCIAAGKPVITATQMLESMVQNPSPTRAEASDVAN
EEEECCCCCCCCHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHH
AIFDGTDAVMLSAETAAGAYPVEAVAMMARIAKAVESSPEFLQKLNVLRPAPTPTTQDAI
HHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHH
AQAADDVVEAVGARAIVVFTATGGSARRIARTRPQVPILALTPNPEVRNQLALVWGVYPH
HHHHHHHHHHCCCCEEEEEEECCCCHHHHHHCCCCCEEEEECCCHHHHHHEEEEEECCCC
LAPDPQDTDDMVRIALREVKALGLAQVGDRVVIAAGVPFGVRGTTNLIRVERVG
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCEEEEEECC
>Mature Secondary Structure 
PPFKRTKIVATLGPATDDKEVIRALAEAGADVFRLNFSHGAPEDHRRRVGWVREVAEEL
CCCCCEEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHH
GRTLAVLQDLQGPKIRVGRFREGQVLLRPGQRFVLTAEPVEGDEHRVSVSYKGLPEDVSP
HHHHHHHHHCCCCCEEECCCCCCCEEEECCCEEEEEECCCCCCCEEEEEEECCCCCCCCC
GQILLLDDGRIRLKVLEVRSPEILTEVEVGGVLSNNKGINIPGADLSIPALSEKDIQDLA
CEEEEEECCCEEEEEEEECCCCCEEEEEECCEEECCCCCCCCCCCCCCCCCCCCCHHHHH
LGAELGVDWVAVSFVRTRDDLLLARHYLSRYGSKARLMAKIEKPSAVARFEEILEEADGI
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHCCCE
MVARGDLGVEMPLEEVPIVQKRLILRCIAAGKPVITATQMLESMVQNPSPTRAEASDVAN
EEEECCCCCCCCHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHH
AIFDGTDAVMLSAETAAGAYPVEAVAMMARIAKAVESSPEFLQKLNVLRPAPTPTTQDAI
HHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHH
AQAADDVVEAVGARAIVVFTATGGSARRIARTRPQVPILALTPNPEVRNQLALVWGVYPH
HHHHHHHHHHCCCCEEEEEEECCCCHHHHHHCCCCCEEEEECCCHHHHHHEEEEEECCCC
LAPDPQDTDDMVRIALREVKALGLAQVGDRVVIAAGVPFGVRGTTNLIRVERVG
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8074528; 12948626 [H]