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The map label for this gene is purF [H]

Identifier: 54294563

GI number: 54294563

Start: 1825598

End: 1827097

Strand: Reverse

Name: purF [H]

Synonym: lpl1639

Alternate gene names: 54294563

Gene position: 1827097-1825598 (Counterclockwise)

Preceding gene: 54294564

Following gene: 54294562

Centisome position: 54.61

GC content: 39.8

Gene sequence:

>1500_bases
ATGTGTGGCATTGTAGGAATTTACAGTCATGAACCCGTTGCTGCTGAGCTTTATGATAGCTTGATTCATTTACAGCACCG
GGGGCAAGATGCCTCAGGTATTTTAACCGGAGCTGATCGTTTTTACTTCAAACATGGACTTGGACTGGTCAGAGAGTCTA
TTTTCTCTGAAGATATTCTTAAACTGAAGGGGAATATCGGCATTGCTCATGTGCGTTATCCCACAGCCGGAGGTTATAGT
GAAGCGGATGTTCAGCCTCTCTGGATAGGGAGCCCTCGCGGGATAGCTCTGGCTCATAATGGAAATTTAGTGAATTATGG
AGAGTTGGTTGATGATTTATGCGGAAAACAGCATAGGCATTTAAACTCTACTCTTGATTCAGAGGCACTTTTATTACTTC
TGGCCGACTATTTAGCCAAGGGCTCTTATTCCGATCATGATGAAGAGCAATTCTTTCAATTATTATGTATGGCAGTTAAT
GAAATACATCAACGAGTTCAAGGCTCCTATTCTGTTGTGAGTTTAGTTATTGGCAAAGGTTTGGTTGCATTTAGGGATCC
ACATGGTATTCGTCCTTTAGTTTGGGGTGAGCGAGTCCATGAAAATGGTAGTAAAGATTATATTTTTGCTTCAGAAACCA
CGCCTTTTTATGCTTTAGGTTTTGAACCCAAGGGTGATTTGCTGCCAGGTGAAGTTGCTTACGTAAATCAGCAAGGACAA
TTGTACAGAAAAGTGCTTTCATCAAAAAAATTTACCCCTTGTATATTTGAATACGTTTACTTCGCCAGACCAGATGCCAC
ATTAGACGATGTGAGCGTTTATCGAGCCAGATTACGTATGGGGCAAAATTTAGCTATAAGCTGGAAAAAGAGATTTCCGG
ATACACTACCTGATGTGGTGATTCCAGCCCCTTCAACAGCAAATACTGCCGCATTAGCTTTTGCAAATGAGTTGGGAATA
CGTTATTCCGAAGGTTTATATAAAAATCCTTTTATTGGACGTACTTTTATCATGCCTAATCAGGAGTCGCGATCAAGACA
GGTTCGCTATAAATTAACTCCTCAGAAAACGGAAATTAATAAAAAAAAGGTATTAATCGTTGATGATAGTATCGTAAGGG
GAACTACCTCACGCGAAATAGTCAGAATGATACGAGAATATGGAGCATCAGAAATATATTTTGTATCAACCTGTCCACCA
ATTAAAAACCCTTGTTTCTACGGGATTGATATCCCATCGCGTAAAAAATTGATTGCAGCCCATAAAACGGAAGAGGAAAT
CCGTCAATTTCTAGGGGTGGATAAATTATTATACCAAACTCAGGAAAATCTTATAGAGGCTGTGTCTCGCAGGGGGCAAT
ATGACATGAGCAAGCCTTGTATGGCTTGTATGGATGGCAAATACATTTGTGGACAGATTACTGAAGAAAAAATTAAATTG
TTGGAGAAAAAAAGATTAACCGAGAATGACCAAGAAACAGAGATAGGAGAGATGTCTTGA

Upstream 100 bases:

>100_bases
GAATCGTTTGTGTATGACAGTAATCCTGGGCCAGTAAATGATAGGATTTTGCATAACATACAACATTGGTTAGGTTAATT
TTTAGCCGGGGGAAAGGCTT

Downstream 100 bases:

>100_bases
TCAATATCCTTATTGTTGGTTCAGGTGCACGTGAGCATGCAATAGCTACAGCATTGAGTAAATCAGAGAATAAACCAAAG
ATTTATTGCTTTGGAACAAC

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MCGIVGIYSHEPVAAELYDSLIHLQHRGQDASGILTGADRFYFKHGLGLVRESIFSEDILKLKGNIGIAHVRYPTAGGYS
EADVQPLWIGSPRGIALAHNGNLVNYGELVDDLCGKQHRHLNSTLDSEALLLLLADYLAKGSYSDHDEEQFFQLLCMAVN
EIHQRVQGSYSVVSLVIGKGLVAFRDPHGIRPLVWGERVHENGSKDYIFASETTPFYALGFEPKGDLLPGEVAYVNQQGQ
LYRKVLSSKKFTPCIFEYVYFARPDATLDDVSVYRARLRMGQNLAISWKKRFPDTLPDVVIPAPSTANTAALAFANELGI
RYSEGLYKNPFIGRTFIMPNQESRSRQVRYKLTPQKTEINKKKVLIVDDSIVRGTTSREIVRMIREYGASEIYFVSTCPP
IKNPCFYGIDIPSRKKLIAAHKTEEEIRQFLGVDKLLYQTQENLIEAVSRRGQYDMSKPCMACMDGKYICGQITEEKIKL
LEKKRLTENDQETEIGEMS

Sequences:

>Translated_499_residues
MCGIVGIYSHEPVAAELYDSLIHLQHRGQDASGILTGADRFYFKHGLGLVRESIFSEDILKLKGNIGIAHVRYPTAGGYS
EADVQPLWIGSPRGIALAHNGNLVNYGELVDDLCGKQHRHLNSTLDSEALLLLLADYLAKGSYSDHDEEQFFQLLCMAVN
EIHQRVQGSYSVVSLVIGKGLVAFRDPHGIRPLVWGERVHENGSKDYIFASETTPFYALGFEPKGDLLPGEVAYVNQQGQ
LYRKVLSSKKFTPCIFEYVYFARPDATLDDVSVYRARLRMGQNLAISWKKRFPDTLPDVVIPAPSTANTAALAFANELGI
RYSEGLYKNPFIGRTFIMPNQESRSRQVRYKLTPQKTEINKKKVLIVDDSIVRGTTSREIVRMIREYGASEIYFVSTCPP
IKNPCFYGIDIPSRKKLIAAHKTEEEIRQFLGVDKLLYQTQENLIEAVSRRGQYDMSKPCMACMDGKYICGQITEEKIKL
LEKKRLTENDQETEIGEMS
>Mature_499_residues
MCGIVGIYSHEPVAAELYDSLIHLQHRGQDASGILTGADRFYFKHGLGLVRESIFSEDILKLKGNIGIAHVRYPTAGGYS
EADVQPLWIGSPRGIALAHNGNLVNYGELVDDLCGKQHRHLNSTLDSEALLLLLADYLAKGSYSDHDEEQFFQLLCMAVN
EIHQRVQGSYSVVSLVIGKGLVAFRDPHGIRPLVWGERVHENGSKDYIFASETTPFYALGFEPKGDLLPGEVAYVNQQGQ
LYRKVLSSKKFTPCIFEYVYFARPDATLDDVSVYRARLRMGQNLAISWKKRFPDTLPDVVIPAPSTANTAALAFANELGI
RYSEGLYKNPFIGRTFIMPNQESRSRQVRYKLTPQKTEINKKKVLIVDDSIVRGTTSREIVRMIREYGASEIYFVSTCPP
IKNPCFYGIDIPSRKKLIAAHKTEEEIRQFLGVDKLLYQTQENLIEAVSRRGQYDMSKPCMACMDGKYICGQITEEKIKL
LEKKRLTENDQETEIGEMS

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=494, Percent_Identity=34.0080971659919, Blast_Score=247, Evalue=2e-65,
Organism=Escherichia coli, GI1788651, Length=488, Percent_Identity=43.2377049180328, Blast_Score=402, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17554892, Length=492, Percent_Identity=35.5691056910569, Blast_Score=251, Evalue=6e-67,
Organism=Saccharomyces cerevisiae, GI6323958, Length=494, Percent_Identity=44.331983805668, Blast_Score=392, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24659598, Length=470, Percent_Identity=32.5531914893617, Blast_Score=236, Evalue=4e-62,
Organism=Drosophila melanogaster, GI28573187, Length=489, Percent_Identity=32.719836400818, Blast_Score=235, Evalue=5e-62,
Organism=Drosophila melanogaster, GI24659604, Length=470, Percent_Identity=32.5531914893617, Blast_Score=235, Evalue=6e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 56239; Mature: 56239

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIVGIYSHEPVAAELYDSLIHLQHRGQDASGILTGADRFYFKHGLGLVRESIFSEDIL
CCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHH
KLKGNIGIAHVRYPTAGGYSEADVQPLWIGSPRGIALAHNGNLVNYGELVDDLCGKQHRH
HHCCCEEEEEEECCCCCCCCCCCCCEEEECCCCCEEEEECCCEEEHHHHHHHHHCHHHHH
LNSTLDSEALLLLLADYLAKGSYSDHDEEQFFQLLCMAVNEIHQRVQGSYSVVSLVIGKG
HHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCC
LVAFRDPHGIRPLVWGERVHENGSKDYIFASETTPFYALGFEPKGDLLPGEVAYVNQQGQ
EEEEECCCCCCCCCCCHHHHCCCCCCEEEEECCCCEEEECCCCCCCCCCCCEEEECCCHH
LYRKVLSSKKFTPCIFEYVYFARPDATLDDVSVYRARLRMGQNLAISWKKRFPDTLPDVV
HHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEEHHHCCCCCCCEE
IPAPSTANTAALAFANELGIRYSEGLYKNPFIGRTFIMPNQESRSRQVRYKLTPQKTEIN
ECCCCCCCHHHHHHHHHHCCEECCCCCCCCCCCEEEECCCCCCCCCEEEEEECCCHHCCC
KKKVLIVDDSIVRGTTSREIVRMIREYGASEIYFVSTCPPIKNPCFYGIDIPSRKKLIAA
CCEEEEECCCHHCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEECCCCCCCHHEEC
HKTEEEIRQFLGVDKLLYQTQENLIEAVSRRGQYDMSKPCMACMDGKYICGQITEEKIKL
CCCHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCEEECCCCHHHHHH
LEKKRLTENDQETEIGEMS
HHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MCGIVGIYSHEPVAAELYDSLIHLQHRGQDASGILTGADRFYFKHGLGLVRESIFSEDIL
CCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHH
KLKGNIGIAHVRYPTAGGYSEADVQPLWIGSPRGIALAHNGNLVNYGELVDDLCGKQHRH
HHCCCEEEEEEECCCCCCCCCCCCCEEEECCCCCEEEEECCCEEEHHHHHHHHHCHHHHH
LNSTLDSEALLLLLADYLAKGSYSDHDEEQFFQLLCMAVNEIHQRVQGSYSVVSLVIGKG
HHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCC
LVAFRDPHGIRPLVWGERVHENGSKDYIFASETTPFYALGFEPKGDLLPGEVAYVNQQGQ
EEEEECCCCCCCCCCCHHHHCCCCCCEEEEECCCCEEEECCCCCCCCCCCCEEEECCCHH
LYRKVLSSKKFTPCIFEYVYFARPDATLDDVSVYRARLRMGQNLAISWKKRFPDTLPDVV
HHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEEHHHCCCCCCCEE
IPAPSTANTAALAFANELGIRYSEGLYKNPFIGRTFIMPNQESRSRQVRYKLTPQKTEIN
ECCCCCCCHHHHHHHHHHCCEECCCCCCCCCCCEEEECCCCCCCCCEEEEEECCCHHCCC
KKKVLIVDDSIVRGTTSREIVRMIREYGASEIYFVSTCPPIKNPCFYGIDIPSRKKLIAA
CCEEEEECCCHHCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEECCCCCCCHHEEC
HKTEEEIRQFLGVDKLLYQTQENLIEAVSRRGQYDMSKPCMACMDGKYICGQITEEKIKL
CCCHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCEEECCCCHHHHHH
LEKKRLTENDQETEIGEMS
HHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043; 7704274 [H]