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The map label for this gene is dusA [H]

Identifier: 54294497

GI number: 54294497

Start: 1745356

End: 1746348

Strand: Reverse

Name: dusA [H]

Synonym: lpl1566

Alternate gene names: 54294497

Gene position: 1746348-1745356 (Counterclockwise)

Preceding gene: 54294498

Following gene: 54294496

Centisome position: 52.2

GC content: 35.85

Gene sequence:

>993_bases
GTGGTTAATAGTATTTTTTCCAAATTATCTATAGCGCCAATGATTGATTGGACTAACAGTCCATTTCGAACTCTGATGAG
GTTATTGGCTCCTAACGCCCTTTTGTATACTGAAATGCAAACAACAGGGGCTGTGCAAAATAATCCTGACAGAGCTTTGT
ATTTTAATTCTGTTGAACATCCTATCGCTATTCAATTGGGTGGGGCAGATAAATCCGCATTAGTTAAGTGTGCCATTATT
GCTGAACAGAAAGGTTTTAATGAAATTAATTTGAATTTGGGATGTCCTAGTGACAAAGTACAGGCGGGACGATTTGGAGC
CTGCTTGATGAAGGAGCCAGAACATGTTGCTGATTGTATTCGTGCAATAAAACAGGTAGTAGGAATACCAGTCACAGCGA
AAACGAGGATCGGTATTGATAACCAGGACAGTTATGAGTTTTTCAGATCTTTTTCTCTTCACCTGGTTGAAGCAGGCTGT
GATAAGCTTATTATTCATGCTCGCAAGGCTTGGTTAAATGGGTTAAATCCAAAGCAAAATAGAACGATTCCTCCCGTAAA
TTATGATTATGTATATGAACTAAAAAAAGAAATTCCTCATATTTTTATTACTATCAATGGGAATATATTAACTGTTGAAG
AAATAAACAGTCATTTAAAACATGTCGATGGAGTAATGTTAGGACGGCTTGCTTGCGATAACCCCTATCAAATTGCTGAA
ATTCATCAAGCCTTATACCCGGAATTCATCAAAATTAAACGCAGTCAGTTGTTAATAAATTATCTGGATTATTGGTTTGA
TCAATCTCGAGCCAATCTCTCGTTAAGCATTTTGGTTAAGCCACTTTTCAATTTAGCTTTTGGATTACCGGGAGCCAAAC
ATTGGAAAAAGAAACTGATGAATATTATTCAGGAGAAAAATACTTCCGGTTTTAGTGAGTTAATCCAGTATTTATTAAAT
TTGGAAGCAATAAATCAAGTCGAATTTGTATAA

Upstream 100 bases:

>100_bases
GAATCCTGTTCAGCAAGCATAAACTCTTAAATTGATGCAATTTTTGTGTATTTCTCAAACAACAATGTATAATGTTATAT
TTGCTTTAAATTGATTAAAC

Downstream 100 bases:

>100_bases
GAAATAATAGAGCAGATGTCAACATTTCGTGGAATTTTATCTAGATTTCTAGTACATTAACGATGTTTATTAATTGGAAC
ATCCTGAGGGTTATTGAGGC

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MVNSIFSKLSIAPMIDWTNSPFRTLMRLLAPNALLYTEMQTTGAVQNNPDRALYFNSVEHPIAIQLGGADKSALVKCAII
AEQKGFNEINLNLGCPSDKVQAGRFGACLMKEPEHVADCIRAIKQVVGIPVTAKTRIGIDNQDSYEFFRSFSLHLVEAGC
DKLIIHARKAWLNGLNPKQNRTIPPVNYDYVYELKKEIPHIFITINGNILTVEEINSHLKHVDGVMLGRLACDNPYQIAE
IHQALYPEFIKIKRSQLLINYLDYWFDQSRANLSLSILVKPLFNLAFGLPGAKHWKKKLMNIIQEKNTSGFSELIQYLLN
LEAINQVEFV

Sequences:

>Translated_330_residues
MVNSIFSKLSIAPMIDWTNSPFRTLMRLLAPNALLYTEMQTTGAVQNNPDRALYFNSVEHPIAIQLGGADKSALVKCAII
AEQKGFNEINLNLGCPSDKVQAGRFGACLMKEPEHVADCIRAIKQVVGIPVTAKTRIGIDNQDSYEFFRSFSLHLVEAGC
DKLIIHARKAWLNGLNPKQNRTIPPVNYDYVYELKKEIPHIFITINGNILTVEEINSHLKHVDGVMLGRLACDNPYQIAE
IHQALYPEFIKIKRSQLLINYLDYWFDQSRANLSLSILVKPLFNLAFGLPGAKHWKKKLMNIIQEKNTSGFSELIQYLLN
LEAINQVEFV
>Mature_330_residues
MVNSIFSKLSIAPMIDWTNSPFRTLMRLLAPNALLYTEMQTTGAVQNNPDRALYFNSVEHPIAIQLGGADKSALVKCAII
AEQKGFNEINLNLGCPSDKVQAGRFGACLMKEPEHVADCIRAIKQVVGIPVTAKTRIGIDNQDSYEFFRSFSLHLVEAGC
DKLIIHARKAWLNGLNPKQNRTIPPVNYDYVYELKKEIPHIFITINGNILTVEEINSHLKHVDGVMLGRLACDNPYQIAE
IHQALYPEFIKIKRSQLLINYLDYWFDQSRANLSLSILVKPLFNLAFGLPGAKHWKKKLMNIIQEKNTSGFSELIQYLLN
LEAINQVEFV

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=265, Percent_Identity=23.3962264150943, Blast_Score=82, Evalue=9e-16,
Organism=Homo sapiens, GI239788462, Length=268, Percent_Identity=23.134328358209, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI31742496, Length=236, Percent_Identity=27.5423728813559, Blast_Score=72, Evalue=9e-13,
Organism=Escherichia coli, GI145693211, Length=298, Percent_Identity=46.6442953020134, Blast_Score=293, Evalue=1e-80,
Organism=Escherichia coli, GI1789660, Length=245, Percent_Identity=26.1224489795918, Blast_Score=75, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6323560, Length=263, Percent_Identity=26.615969581749, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24580595, Length=234, Percent_Identity=27.7777777777778, Blast_Score=81, Evalue=9e-16,
Organism=Drosophila melanogaster, GI19920448, Length=234, Percent_Identity=27.7777777777778, Blast_Score=81, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 37312; Mature: 37312

Theoretical pI: Translated: 8.35; Mature: 8.35

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNSIFSKLSIAPMIDWTNSPFRTLMRLLAPNALLYTEMQTTGAVQNNPDRALYFNSVEH
CCHHHHHHHCCCEEECCCCCHHHHHHHHHCCCCEEEEEHHHCCCCCCCCCCEEEECCCCC
PIAIQLGGADKSALVKCAIIAEQKGFNEINLNLGCPSDKVQAGRFGACLMKEPEHVADCI
CEEEEECCCCHHHHEEEEEEECCCCCCEEEEEECCCCCCCCCCCCCHHHCCCCHHHHHHH
RAIKQVVGIPVTAKTRIGIDNQDSYEFFRSFSLHLVEAGCDKLIIHARKAWLNGLNPKQN
HHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCC
RTIPPVNYDYVYELKKEIPHIFITINGNILTVEEINSHLKHVDGVMLGRLACDNPYQIAE
CCCCCCCHHHHHHHHHHCCEEEEEECCCEEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
IHQALYPEFIKIKRSQLLINYLDYWFDQSRANLSLSILVKPLFNLAFGLPGAKHWKKKLM
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHCCCCHHHHHHHHH
NIIQEKNTSGFSELIQYLLNLEAINQVEFV
HHHHHCCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MVNSIFSKLSIAPMIDWTNSPFRTLMRLLAPNALLYTEMQTTGAVQNNPDRALYFNSVEH
CCHHHHHHHCCCEEECCCCCHHHHHHHHHCCCCEEEEEHHHCCCCCCCCCCEEEECCCCC
PIAIQLGGADKSALVKCAIIAEQKGFNEINLNLGCPSDKVQAGRFGACLMKEPEHVADCI
CEEEEECCCCHHHHEEEEEEECCCCCCEEEEEECCCCCCCCCCCCCHHHCCCCHHHHHHH
RAIKQVVGIPVTAKTRIGIDNQDSYEFFRSFSLHLVEAGCDKLIIHARKAWLNGLNPKQN
HHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCC
RTIPPVNYDYVYELKKEIPHIFITINGNILTVEEINSHLKHVDGVMLGRLACDNPYQIAE
CCCCCCCHHHHHHHHHHCCEEEEEECCCEEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
IHQALYPEFIKIKRSQLLINYLDYWFDQSRANLSLSILVKPLFNLAFGLPGAKHWKKKLM
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHCCCCHHHHHHHHH
NIIQEKNTSGFSELIQYLLNLEAINQVEFV
HHHHHCCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]