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The map label for this gene is bioH [H]

Identifier: 54294485

GI number: 54294485

Start: 1731192

End: 1731911

Strand: Reverse

Name: bioH [H]

Synonym: lpl1554

Alternate gene names: 54294485

Gene position: 1731911-1731192 (Counterclockwise)

Preceding gene: 54294486

Following gene: 54294484

Centisome position: 51.77

GC content: 37.64

Gene sequence:

>720_bases
ATGAATATTCATCTCGATAAACATGGGCAAGGAATGCCTTTGGTCCTATTTCATGGCTGGGGATTTGATAGCCAAATCTG
GCAACCGATAATTCCTTACCTCAAGCCTAAATATCAGATTATTTTGGTCGATTTACCTGGTTTTGGATTGACTCCAATGA
TGGACTGGGAAAGTTTTAAAAAGAATTTGTTTGACCAATTACCTGATAAATTTGCATTGGCAGGGTGGTCAATGGGTGGG
CTGTACGCGACACGTTTAGCAATTGAAGAGCCTGCCAGAGTGCAGTATTTGATAAATATAACGTCTTCACCTCGCTTTAT
CTCTGATATTGACTGGCCGGGAGTAGCAGAGGAGGTATTTGTCAATTTTTATAACAATTTATCGAAAGATATTAATAAGA
CATTAAAAGAGTTCATATCCTTGCAACTCAATAAGATGAAATTTGATTTTAAAATAGGTAATCCTCCATCTCCAGAAGGC
TTGGCGTTTGGTCTCGAAATTTTGGGTACCTGGGATTTTAGAGAGCAGTTGAAACAAATATCTATACCTACTGTTTACTT
ATTTGGTCGACTTGATCCGATTACTCCTGCCAAGACCATGGCAATTATGGAGAAAAATTACCCGAATTTTAAGTATGTCC
TCTTCAACAGAGCAGCTCACATGCCTTTTCTGTCCCATACCGATTTATTTATTACAATGATGGATGAATTTATAAAATGA

Upstream 100 bases:

>100_bases
CCAACGGTAAATAGTGCTTCTTCCGGTTTACGGATTATTATTAATTATAAGCATACTATTGAACAAATCATTGAGTTATT
TAATAAATTGCACTTAATAC

Downstream 100 bases:

>100_bases
AACGATATTTTGTTACGGGTACTGACACTGATTGTGGTAAGACATTTGTAACCAATCAATTAGTTAATTATTTTTCAAGT
TCGGCAGCGATAAAACCTAT

Product: biotin biosynthesis protein BioH

Products: NA

Alternate protein names: Biotin synthesis protein BioH [H]

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MNIHLDKHGQGMPLVLFHGWGFDSQIWQPIIPYLKPKYQIILVDLPGFGLTPMMDWESFKKNLFDQLPDKFALAGWSMGG
LYATRLAIEEPARVQYLINITSSPRFISDIDWPGVAEEVFVNFYNNLSKDINKTLKEFISLQLNKMKFDFKIGNPPSPEG
LAFGLEILGTWDFREQLKQISIPTVYLFGRLDPITPAKTMAIMEKNYPNFKYVLFNRAAHMPFLSHTDLFITMMDEFIK

Sequences:

>Translated_239_residues
MNIHLDKHGQGMPLVLFHGWGFDSQIWQPIIPYLKPKYQIILVDLPGFGLTPMMDWESFKKNLFDQLPDKFALAGWSMGG
LYATRLAIEEPARVQYLINITSSPRFISDIDWPGVAEEVFVNFYNNLSKDINKTLKEFISLQLNKMKFDFKIGNPPSPEG
LAFGLEILGTWDFREQLKQISIPTVYLFGRLDPITPAKTMAIMEKNYPNFKYVLFNRAAHMPFLSHTDLFITMMDEFIK
>Mature_239_residues
MNIHLDKHGQGMPLVLFHGWGFDSQIWQPIIPYLKPKYQIILVDLPGFGLTPMMDWESFKKNLFDQLPDKFALAGWSMGG
LYATRLAIEEPARVQYLINITSSPRFISDIDWPGVAEEVFVNFYNNLSKDINKTLKEFISLQLNKMKFDFKIGNPPSPEG
LAFGLEILGTWDFREQLKQISIPTVYLFGRLDPITPAKTMAIMEKNYPNFKYVLFNRAAHMPFLSHTDLFITMMDEFIK

Specific function: Shows carboxylesterase activity with a preference for short chain fatty acid esters (acyl chain length of up to 6 carbons). Also displays a weak thioesterase activity. Can form a complex with CoA, and may be involved in the condensation of CoA and pimelic

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family [H]

Homologues:

Organism=Escherichia coli, GI1789817, Length=247, Percent_Identity=35.6275303643725, Blast_Score=161, Evalue=4e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR010076 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.1.1.1 [H]

Molecular weight: Translated: 27650; Mature: 27650

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIHLDKHGQGMPLVLFHGWGFDSQIWQPIIPYLKPKYQIILVDLPGFGLTPMMDWESFK
CEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHH
KNLFDQLPDKFALAGWSMGGLYATRLAIEEPARVQYLINITSSPRFISDIDWPGVAEEVF
HHHHHHCCHHHHHCCCCCCCHHEEHEECCCCCEEEEEEEECCCCCEECCCCCCCHHHHHH
VNFYNNLSKDINKTLKEFISLQLNKMKFDFKIGNPPSPEGLAFGLEILGTWDFREQLKQI
HHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCEEEEHEEECCHHHHHHHHC
SIPTVYLFGRLDPITPAKTMAIMEKNYPNFKYVLFNRAAHMPFLSHTDLFITMMDEFIK
CCCEEEEEECCCCCCCHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MNIHLDKHGQGMPLVLFHGWGFDSQIWQPIIPYLKPKYQIILVDLPGFGLTPMMDWESFK
CEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHH
KNLFDQLPDKFALAGWSMGGLYATRLAIEEPARVQYLINITSSPRFISDIDWPGVAEEVF
HHHHHHCCHHHHHCCCCCCCHHEEHEECCCCCEEEEEEEECCCCCEECCCCCCCHHHHHH
VNFYNNLSKDINKTLKEFISLQLNKMKFDFKIGNPPSPEGLAFGLEILGTWDFREQLKQI
HHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCEEEEHEEECCHHHHHHHHC
SIPTVYLFGRLDPITPAKTMAIMEKNYPNFKYVLFNRAAHMPFLSHTDLFITMMDEFIK
CCCEEEEEECCCCCCCHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA