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The map label for this gene is ygfZ [C]

Identifier: 54294377

GI number: 54294377

Start: 1614884

End: 1615942

Strand: Reverse

Name: ygfZ [C]

Synonym: lpl1446

Alternate gene names: 54294377

Gene position: 1615942-1614884 (Counterclockwise)

Preceding gene: 54294387

Following gene: 54294372

Centisome position: 48.3

GC content: 32.29

Gene sequence:

>1059_bases
ATGATATCTGATTTTGAGAGGCCAAATGATACACTACTCTGTATTATAAACAACATCACTGATCTTGATATGAAAACTAT
GTTAGAACATTTAGTGAATAATAGGTCTTTAATAACATTCCACCCTATAGAGTCTGAATTATTATTAAATAAGCAGAAAA
ACTATCTTTTTGACTTATCCTATCTAGGTGTCATCGATTTGTTTGGAGAGAAATCAATAGATTTTCTGCAAGGGCAGTTA
ACTTGTGATTTACGATTGGTATCGGATGTTTCAATGGTTCAAGGGGCTCAATGTAACCTTAAAGGGAGAATTCTTTCCTT
ATTGGATATTATCAACTGGCAAGGTGTAAAATTAGTTCTGCCCCAAGATTTAATTGAAGTAACTCAGAATTCATTAAACA
AAGTTGCACTGCTTTCTCGTGTTAAAATCACATCAAATAACAACTATAAAATTTTAGGCTTTTATTTACAGCATGATAAT
GATCAAATACCACAGCTACTACCTTTGCCCAGCGAATTGTATGCTTTATCTTGTACGACTCATGGTTGTGTTTATCACTT
AGGTAAAGGATTCTATATCTATTTAATCCATTCAGACTATTATGATAGTTTGTGCAAGCCTTTTATTGAAAATAATCAAT
TACTTGGTTCATTAACCTGGCATACTCTTAGATTATTTAACAATCAAATTGATATTTATCCTAATTCTAGGGGGCTCTTT
TTACCACATAGAATTGGCTTACATCAAACCACGTACGTCAGTTTTGATAAAGGGTGTTATAAAGGTCAGGAAATCATCGC
AAGAACTCACTATAGAGCCACTCTTAAGCATGAATTAAAGAAATTTGTGATTCAGTCAGATAATCAATTATATTCTGGTC
AAAAATTATTTAAATCGGATGAAAACACAGAGGTTGGAGAGTTAATTGATTACTCCATGCTTGGTGAAAATCGATATTTG
GTTGCTGCAAGTATATTAAAAGAGAACTTTTCATCTGTCTGTTTGGAAGGCAATAAATTTCCAATCCAATTAGAAGGAAA
AATTGAGCTTGAACTTTGA

Upstream 100 bases:

>100_bases
CGCCTGCATTTCCATAATAACCTTGATTTTTCTCTATTGTCCACAATGGATTTCCTCTGTTACTTCACGATAAATACAAC
ATAACCCTAATTATTCAATA

Downstream 100 bases:

>100_bases
AGTTCAGGCTCAATTTTCAAAAACAGAGGGCTATTTGCTTTTTCCAGGCAGCATACGCTTCAATACATTGTCTTTATCAA
TAAAATGATGCTTAAGAGCA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MISDFERPNDTLLCIINNITDLDMKTMLEHLVNNRSLITFHPIESELLLNKQKNYLFDLSYLGVIDLFGEKSIDFLQGQL
TCDLRLVSDVSMVQGAQCNLKGRILSLLDIINWQGVKLVLPQDLIEVTQNSLNKVALLSRVKITSNNNYKILGFYLQHDN
DQIPQLLPLPSELYALSCTTHGCVYHLGKGFYIYLIHSDYYDSLCKPFIENNQLLGSLTWHTLRLFNNQIDIYPNSRGLF
LPHRIGLHQTTYVSFDKGCYKGQEIIARTHYRATLKHELKKFVIQSDNQLYSGQKLFKSDENTEVGELIDYSMLGENRYL
VAASILKENFSSVCLEGNKFPIQLEGKIELEL

Sequences:

>Translated_352_residues
MISDFERPNDTLLCIINNITDLDMKTMLEHLVNNRSLITFHPIESELLLNKQKNYLFDLSYLGVIDLFGEKSIDFLQGQL
TCDLRLVSDVSMVQGAQCNLKGRILSLLDIINWQGVKLVLPQDLIEVTQNSLNKVALLSRVKITSNNNYKILGFYLQHDN
DQIPQLLPLPSELYALSCTTHGCVYHLGKGFYIYLIHSDYYDSLCKPFIENNQLLGSLTWHTLRLFNNQIDIYPNSRGLF
LPHRIGLHQTTYVSFDKGCYKGQEIIARTHYRATLKHELKKFVIQSDNQLYSGQKLFKSDENTEVGELIDYSMLGENRYL
VAASILKENFSSVCLEGNKFPIQLEGKIELEL
>Mature_352_residues
MISDFERPNDTLLCIINNITDLDMKTMLEHLVNNRSLITFHPIESELLLNKQKNYLFDLSYLGVIDLFGEKSIDFLQGQL
TCDLRLVSDVSMVQGAQCNLKGRILSLLDIINWQGVKLVLPQDLIEVTQNSLNKVALLSRVKITSNNNYKILGFYLQHDN
DQIPQLLPLPSELYALSCTTHGCVYHLGKGFYIYLIHSDYYDSLCKPFIENNQLLGSLTWHTLRLFNNQIDIYPNSRGLF
LPHRIGLHQTTYVSFDKGCYKGQEIIARTHYRATLKHELKKFVIQSDNQLYSGQKLFKSDENTEVGELIDYSMLGENRYL
VAASILKENFSSVCLEGNKFPIQLEGKIELEL

Specific function: Folate-binding protein involved in regulating the level of ATP-dnaA and in the modification of some tRNAs. It is probably a key factor in regulatory networks that act via tRNA modification, such as initiation of chromosomal replication [H]

COG id: COG0354

COG function: function code R; Predicted aminomethyltransferase related to GcvT

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA-modifying ygfZ family [H]

Homologues:

None

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017703
- InterPro:   IPR006222 [H]

Pfam domain/function: PF01571 GCV_T [H]

EC number: NA

Molecular weight: Translated: 40423; Mature: 40423

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISDFERPNDTLLCIINNITDLDMKTMLEHLVNNRSLITFHPIESELLLNKQKNYLFDLS
CCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCEEEEECCCCHHHHHCCCCCEEEEEH
YLGVIDLFGEKSIDFLQGQLTCDLRLVSDVSMVQGAQCNLKGRILSLLDIINWQGVKLVL
HHHHHHHCCCCCCHHHCCEEEEEEEEECCHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEC
PQDLIEVTQNSLNKVALLSRVKITSNNNYKILGFYLQHDNDQIPQLLPLPSELYALSCTT
CHHHHHHHHHHHHHEEEEEEEEEECCCCEEEEEEEEECCCCCCCCCCCCCHHHEEEEECC
HGCVYHLGKGFYIYLIHSDYYDSLCKPFIENNQLLGSLTWHTLRLFNNQIDIYPNSRGLF
CCEEEECCCCEEEEEEECCHHHHHHHHHHCCCCEEEHHHHHEEEEECCEEEEEECCCCEE
LPHRIGLHQTTYVSFDKGCYKGQEIIARTHYRATLKHELKKFVIQSDNQLYSGQKLFKSD
CCEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCHHHHCCC
ENTEVGELIDYSMLGENRYLVAASILKENFSSVCLEGNKFPIQLEGKIELEL
CCCCHHHHHHHHHCCCCCEEEEHHHHHHCHHHHEECCCCCEEEECCEEEEEC
>Mature Secondary Structure
MISDFERPNDTLLCIINNITDLDMKTMLEHLVNNRSLITFHPIESELLLNKQKNYLFDLS
CCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCEEEEECCCCHHHHHCCCCCEEEEEH
YLGVIDLFGEKSIDFLQGQLTCDLRLVSDVSMVQGAQCNLKGRILSLLDIINWQGVKLVL
HHHHHHHCCCCCCHHHCCEEEEEEEEECCHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEC
PQDLIEVTQNSLNKVALLSRVKITSNNNYKILGFYLQHDNDQIPQLLPLPSELYALSCTT
CHHHHHHHHHHHHHEEEEEEEEEECCCCEEEEEEEEECCCCCCCCCCCCCHHHEEEEECC
HGCVYHLGKGFYIYLIHSDYYDSLCKPFIENNQLLGSLTWHTLRLFNNQIDIYPNSRGLF
CCEEEECCCCEEEEEEECCHHHHHHHHHHCCCCEEEHHHHHEEEEECCEEEEEECCCCEE
LPHRIGLHQTTYVSFDKGCYKGQEIIARTHYRATLKHELKKFVIQSDNQLYSGQKLFKSD
CCEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCHHHHCCC
ENTEVGELIDYSMLGENRYLVAASILKENFSSVCLEGNKFPIQLEGKIELEL
CCCCHHHHHHHHHCCCCCEEEEHHHHHHCHHHHEECCCCCEEEECCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA