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The map label for this gene is phrB [H]

Identifier: 54293218

GI number: 54293218

Start: 297965

End: 299380

Strand: Reverse

Name: phrB [H]

Synonym: lpl0266

Alternate gene names: 54293218

Gene position: 299380-297965 (Counterclockwise)

Preceding gene: 54293219

Following gene: 54293217

Centisome position: 8.95

GC content: 42.3

Gene sequence:

>1416_bases
ATGACTATTGCTCTTGTGTGGTTCCGTCAAGATTTGAGATTGACCGATAATCCCGCGTTTATCGAAGCATGCTCCCATCA
TGAAATTGTAATTCCACTTTATATTTATGATGATAAATGCAGTGTTTTAGGGCAAGCTCAAGCCTGGTGGCTCCATCATA
GTCTCATTGCTCTGGGCAAATCGTTAAATCAACAGGGTTTATGCCTCGTTCTTCGCAAAGGAGCCCCGCAAGAGATCATT
TTGGAGTTAGTCGCACAGTATGGTGTGGAATCAGTCTATTGGAATCGCTGCTATGAACCACAGGCCATTGCACGTGATAA
AAAAATTAAGCTTGCTTTGGGCGAAAGAGAAATTAAAGTGCAGAGTTTCAATGGAAATTTGCTTCATGAGCCGTGGACAA
TTAAAAATAAAAATGGGGATTTTTTTAAGGTTTTTACTCCTTACTGGAAACAATGTCGACAAATTCTTAATATTCCCTCG
CCTTTTATGCTCACTAAACGCCCAAGGGGAATCAAAATTACAAGTGATGACCTTGGCAAATGGAAATTATTGCCAGCAAT
TAATTGGGCAGCACGATTTTCTGAATATTGGACTCCAGGAGAAGAGGGCGCACAAAAAAAACTGGATGAGTTTATTGTTC
ATCACCTTAGCGGATATAAAAGGGAACGCGATTTTCCAGCTAAAAATGCGACATCTCGATTGTCACCTCATTTGCACTTT
GGGGAAATCAGTCCTTGGGTGATTTTAAGAGCACTGGAGTTGGCTAAACTGGAACAAACCTGTGATCTGGCCTCCGTAGA
ACATTTTTTATCAGAGCTGGGATGGAGAGAGTTTTCTGTCTATCTGCTCTACCACTTCCCCAAGCTGGATTGCGAAAATT
TTAGAAAGGAGTTTGATGCATTCCCCTGGCAAAATGATGAGCAATTATTAACTTGCTGGCAAAAGGGAATGACTGGGTAT
CCTATCATTGATGCTGGAATGAGAGAATTATGGGCCACAGGATACATGCACAACCGCGTTCGTATGATTGTCGCATCATT
TCTTACCAAAGATTTACTGATTGATTGGCGTTCGGGCGCACAGTGGTTTTTAGACACCCTAGTCGATGCGGATCTGGCGA
ATAACAGCGCGAGCTGGCAATGGGTAGCAGGATGCGGTGCGGATGCAGCACCTTATTTCCGTATTTTCAATCCTGTTTTA
CAAAGCCAAAAATTTGACCCGGATGGCGTTTACATACGCCAATGGATCCCCGAACTTGCCCAGTTAAATAGTCAGTCAAT
TCATCAACCCTGGACAGATGCTGACGCCAGTTCCATTTTTTTAGGGAAAAAATATCCCAAACCCATCATTGATCACCAAG
AAGCGAGAACTAAGGCATTGAGTTACTATCAGCAAATAAAAAAAGGAATGGATTGA

Upstream 100 bases:

>100_bases
AAATAAAAGGGCGTTTAGTAGAAATTAAAAGGAACTCCTCTAGTTAAGAAAAAATAATAAGCATGTTATTTTATGGTTTT
TCCATCAATAGAAGAAAAGA

Downstream 100 bases:

>100_bases
CAAGAGATTAACTAAAATTTGGAGAGACCCCATTGAATAAAAAAAGTTGGATGGAACTGATCGCTTGGATAATTACCTTT
GAAGCAATTGGTTTTTTTTT

Product: hypothetical protein

Products: NA

Alternate protein names: DNA photolyase; Photoreactivating enzyme [H]

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MTIALVWFRQDLRLTDNPAFIEACSHHEIVIPLYIYDDKCSVLGQAQAWWLHHSLIALGKSLNQQGLCLVLRKGAPQEII
LELVAQYGVESVYWNRCYEPQAIARDKKIKLALGEREIKVQSFNGNLLHEPWTIKNKNGDFFKVFTPYWKQCRQILNIPS
PFMLTKRPRGIKITSDDLGKWKLLPAINWAARFSEYWTPGEEGAQKKLDEFIVHHLSGYKRERDFPAKNATSRLSPHLHF
GEISPWVILRALELAKLEQTCDLASVEHFLSELGWREFSVYLLYHFPKLDCENFRKEFDAFPWQNDEQLLTCWQKGMTGY
PIIDAGMRELWATGYMHNRVRMIVASFLTKDLLIDWRSGAQWFLDTLVDADLANNSASWQWVAGCGADAAPYFRIFNPVL
QSQKFDPDGVYIRQWIPELAQLNSQSIHQPWTDADASSIFLGKKYPKPIIDHQEARTKALSYYQQIKKGMD

Sequences:

>Translated_471_residues
MTIALVWFRQDLRLTDNPAFIEACSHHEIVIPLYIYDDKCSVLGQAQAWWLHHSLIALGKSLNQQGLCLVLRKGAPQEII
LELVAQYGVESVYWNRCYEPQAIARDKKIKLALGEREIKVQSFNGNLLHEPWTIKNKNGDFFKVFTPYWKQCRQILNIPS
PFMLTKRPRGIKITSDDLGKWKLLPAINWAARFSEYWTPGEEGAQKKLDEFIVHHLSGYKRERDFPAKNATSRLSPHLHF
GEISPWVILRALELAKLEQTCDLASVEHFLSELGWREFSVYLLYHFPKLDCENFRKEFDAFPWQNDEQLLTCWQKGMTGY
PIIDAGMRELWATGYMHNRVRMIVASFLTKDLLIDWRSGAQWFLDTLVDADLANNSASWQWVAGCGADAAPYFRIFNPVL
QSQKFDPDGVYIRQWIPELAQLNSQSIHQPWTDADASSIFLGKKYPKPIIDHQEARTKALSYYQQIKKGMD
>Mature_470_residues
TIALVWFRQDLRLTDNPAFIEACSHHEIVIPLYIYDDKCSVLGQAQAWWLHHSLIALGKSLNQQGLCLVLRKGAPQEIIL
ELVAQYGVESVYWNRCYEPQAIARDKKIKLALGEREIKVQSFNGNLLHEPWTIKNKNGDFFKVFTPYWKQCRQILNIPSP
FMLTKRPRGIKITSDDLGKWKLLPAINWAARFSEYWTPGEEGAQKKLDEFIVHHLSGYKRERDFPAKNATSRLSPHLHFG
EISPWVILRALELAKLEQTCDLASVEHFLSELGWREFSVYLLYHFPKLDCENFRKEFDAFPWQNDEQLLTCWQKGMTGYP
IIDAGMRELWATGYMHNRVRMIVASFLTKDLLIDWRSGAQWFLDTLVDADLANNSASWQWVAGCGADAAPYFRIFNPVLQ
SQKFDPDGVYIRQWIPELAQLNSQSIHQPWTDADASSIFLGKKYPKPIIDHQEARTKALSYYQQIKKGMD

Specific function: Involved in repair of UV radiation-induced DNA damage. Catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ul

COG id: COG0415

COG function: function code L; Deoxyribodipyrimidine photolyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DNA photolyase domain [H]

Homologues:

Organism=Homo sapiens, GI188536100, Length=493, Percent_Identity=29.2089249492901, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI188536103, Length=453, Percent_Identity=28.476821192053, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI4758072, Length=487, Percent_Identity=27.3100616016427, Blast_Score=149, Evalue=4e-36,
Organism=Escherichia coli, GI1786926, Length=483, Percent_Identity=39.3374741200828, Blast_Score=308, Evalue=6e-85,
Organism=Saccharomyces cerevisiae, GI6324962, Length=399, Percent_Identity=28.5714285714286, Blast_Score=177, Evalue=3e-45,
Organism=Drosophila melanogaster, GI17137248, Length=476, Percent_Identity=27.5210084033613, Blast_Score=168, Evalue=7e-42,
Organism=Drosophila melanogaster, GI24585455, Length=476, Percent_Identity=27.5210084033613, Blast_Score=168, Evalue=7e-42,
Organism=Drosophila melanogaster, GI24648152, Length=515, Percent_Identity=25.4368932038835, Blast_Score=153, Evalue=3e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002081
- InterPro:   IPR018394
- InterPro:   IPR006050
- InterPro:   IPR005101
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00875 DNA_photolyase; PF03441 FAD_binding_7 [H]

EC number: =4.1.99.3 [H]

Molecular weight: Translated: 54642; Mature: 54511

Theoretical pI: Translated: 7.79; Mature: 7.79

Prosite motif: PS00639 THIOL_PROTEASE_HIS ; PS00394 DNA_PHOTOLYASES_1_1 ; PS00691 DNA_PHOTOLYASES_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIALVWFRQDLRLTDNPAFIEACSHHEIVIPLYIYDDKCSVLGQAQAWWLHHSLIALGK
CEEEEEEEECCCCCCCCCHHHHHCCCCCEEEEEEEECCCHHHHCCHHHHHHHHHHHHHHH
SLNQQGLCLVLRKGAPQEIILELVAQYGVESVYWNRCYEPQAIARDKKIKLALGEREIKV
CCCCCCEEEEEECCCCHHHHHHHHHHHCCHHHHHHCCCCCHHHCCCCEEEEEECCCEEEE
QSFNGNLLHEPWTIKNKNGDFFKVFTPYWKQCRQILNIPSPFMLTKRPRGIKITSDDLGK
EECCCCEECCCEEEECCCCCEEEECCHHHHHHHHHHCCCCCCEECCCCCCCEEECCCCCC
WKLLPAINWAARFSEYWTPGEEGAQKKLDEFIVHHLSGYKRERDFPAKNATSRLSPHLHF
EEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCC
GEISPWVILRALELAKLEQTCDLASVEHFLSELGWREFSVYLLYHFPKLDCENFRKEFDA
CCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHCC
FPWQNDEQLLTCWQKGMTGYPIIDAGMRELWATGYMHNRVRMIVASFLTKDLLIDWRSGA
CCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCH
QWFLDTLVDADLANNSASWQWVAGCGADAAPYFRIFNPVLQSQKFDPDGVYIRQWIPELA
HHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
QLNSQSIHQPWTDADASSIFLGKKYPKPIIDHQEARTKALSYYQQIKKGMD
HCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TIALVWFRQDLRLTDNPAFIEACSHHEIVIPLYIYDDKCSVLGQAQAWWLHHSLIALGK
EEEEEEEECCCCCCCCCHHHHHCCCCCEEEEEEEECCCHHHHCCHHHHHHHHHHHHHHH
SLNQQGLCLVLRKGAPQEIILELVAQYGVESVYWNRCYEPQAIARDKKIKLALGEREIKV
CCCCCCEEEEEECCCCHHHHHHHHHHHCCHHHHHHCCCCCHHHCCCCEEEEEECCCEEEE
QSFNGNLLHEPWTIKNKNGDFFKVFTPYWKQCRQILNIPSPFMLTKRPRGIKITSDDLGK
EECCCCEECCCEEEECCCCCEEEECCHHHHHHHHHHCCCCCCEECCCCCCCEEECCCCCC
WKLLPAINWAARFSEYWTPGEEGAQKKLDEFIVHHLSGYKRERDFPAKNATSRLSPHLHF
EEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCC
GEISPWVILRALELAKLEQTCDLASVEHFLSELGWREFSVYLLYHFPKLDCENFRKEFDA
CCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHCC
FPWQNDEQLLTCWQKGMTGYPIIDAGMRELWATGYMHNRVRMIVASFLTKDLLIDWRSGA
CCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCH
QWFLDTLVDADLANNSASWQWVAGCGADAAPYFRIFNPVLQSQKFDPDGVYIRQWIPELA
HHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
QLNSQSIHQPWTDADASSIFLGKKYPKPIIDHQEARTKALSYYQQIKKGMD
HCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6325460; 8905232; 9278503; 2200511; 7604260 [H]