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The map label for this gene is hemA

Identifier: 54027144

GI number: 54027144

Start: 5468598

End: 5470031

Strand: Reverse

Name: hemA

Synonym: nfa51700

Alternate gene names: 54027144

Gene position: 5470031-5468598 (Counterclockwise)

Preceding gene: 54027145

Following gene: 54027143

Centisome position: 90.85

GC content: 73.08

Gene sequence:

>1434_bases
ATGAGCGTTCTTCTCGTCGGGGTCTCGCATCGCAGCGCCCCCGTCTCGGTGCTCGAGAAAGTAGCGATCACCGACGCCGA
CCGACCCAAGCTGATCGACAAGATGCTCGCCTCCAGCCACATCTCGGAGGCCATGATCGTCTCGACCTGCAACCGCGTGG
AGGTCTACGCGGTCGTCGACGCCTTCCACGGCGGTCTCGCCGAGGTGGGTGAACTGCTCACCAGGCATTCCGGGCTCGCC
ATGCCCGACCTGACCAAGCACGCCTACGTCCGCTACAGCGAGGCCGCCGCCGAGCACCTGTTCGCGGTGGCCAGCGGGCT
GGACTCGATGGTGGTCGGCGAGCAGCAGGTGCTCAGCCAGATCCGCGGCGCCTACGCCACCGCCGACGCCCAGCAGGCCG
TCGGCCGCACCCTGCACGAGCTGGCCCAGCACGCGCTGCGGGTCGGCAAGCGGGTGCATTCGGAGACCGGCATCGACCGC
GCGGGCGCGTCGGTGGTCTCGGTCGCGCTGGACCGCGCCGCCCAGGTGCTCGGTGACCTGGCCGGACGCACCGCCGTCGT
GGTCGGCGCCGGCGCGATGGGCGGGCTGTCGGTCGCGCACCTGGCGCGCGCCGGGATCGGCCGCATCATCGTGGTCAACC
GGACCATCGAGCGCGCGCGCAGGCTGGCCGAGACGGCGGCCTCCTACGGGGTGGAGTCCTCGGCGCTGGAACTCGACCGC
CTGCACGAGGCGATGTCGGCCGCCGACGTGGTGCTCTCCTGTACCGGCGCCGTCGGCGCCGTGGTGACCCTGGCCGACAC
CCACCGCGCGCTGGCCGACCGGGACCGCGCCGAATTCCCGGCCGGTGACCGGCCGCTGGTGTTCTGCGACCTCGGCCTGC
CGCGTGACGTGGAACCCGCCGTCGCCGGGCTGCCCGGTGTCGCGGTGATCGACATCGAGTCCCTGCAGCGCGATCCGGCG
GCCGGCGCGGCCGCCGACGACACCGCCGCCGCGCGCTCGATCGTCGCCGAAGAACTCGCCAAGTACCTGGCCGGCCAGCG
GATGGCCGAGGTGACCCCGACCGTGGCCGCGCTGCGGCAGCGTGCCGCCGAAGTGGTGGAAGCCGAACTGCTGCGGCTGG
ATTCGCGGCTGCCCGGCCTGGCCGAGCCGGAACGCGACGAGGTCGCGCGCACCGTGCGCCGCGTGGTCGACAAGCTGCTG
CACGCGCCGACCGTGCGGGTCAAGCAGCTGGCCTCCACCCCGGGCGGCGACAGCTACGCCGAGGCCCTGCGCGAGCTGTT
CGAGCTCAAGCCGGGTGCGGCGCAGGCGGTCGCGGCGCCGATGGAGATCACCGCGATCGGCCCCGACCGTACGGCCGGAC
TGGCCGACGACTTCACCCCGGGCCACCGCCTGGATTTCGAGCAGCACCTCGGCGAGGAGGGAAAGCCGGCATGA

Upstream 100 bases:

>100_bases
ACACCCCGGCCGCGCCCGCGGCCATCCAGCGTTCCGCCGCACACGCGGCTACCTCGCACACGGCAATGGAGCCAACCAGC
AAGGGATCGGTGGTCACACC

Downstream 100 bases:

>100_bases
TCGTCGCGCAGGAACAGGGCAACGTGGGCGCCGCGGCGACGGGTACGCCGTTGCGGATCGGCACCCGCGGCAGCCTGCTG
GCGATGACCCAGGCGGGCAC

Product: glutamyl-tRNA reductase

Products: NA

Alternate protein names: GluTR

Number of amino acids: Translated: 477; Mature: 476

Protein sequence:

>477_residues
MSVLLVGVSHRSAPVSVLEKVAITDADRPKLIDKMLASSHISEAMIVSTCNRVEVYAVVDAFHGGLAEVGELLTRHSGLA
MPDLTKHAYVRYSEAAAEHLFAVASGLDSMVVGEQQVLSQIRGAYATADAQQAVGRTLHELAQHALRVGKRVHSETGIDR
AGASVVSVALDRAAQVLGDLAGRTAVVVGAGAMGGLSVAHLARAGIGRIIVVNRTIERARRLAETAASYGVESSALELDR
LHEAMSAADVVLSCTGAVGAVVTLADTHRALADRDRAEFPAGDRPLVFCDLGLPRDVEPAVAGLPGVAVIDIESLQRDPA
AGAAADDTAAARSIVAEELAKYLAGQRMAEVTPTVAALRQRAAEVVEAELLRLDSRLPGLAEPERDEVARTVRRVVDKLL
HAPTVRVKQLASTPGGDSYAEALRELFELKPGAAQAVAAPMEITAIGPDRTAGLADDFTPGHRLDFEQHLGEEGKPA

Sequences:

>Translated_477_residues
MSVLLVGVSHRSAPVSVLEKVAITDADRPKLIDKMLASSHISEAMIVSTCNRVEVYAVVDAFHGGLAEVGELLTRHSGLA
MPDLTKHAYVRYSEAAAEHLFAVASGLDSMVVGEQQVLSQIRGAYATADAQQAVGRTLHELAQHALRVGKRVHSETGIDR
AGASVVSVALDRAAQVLGDLAGRTAVVVGAGAMGGLSVAHLARAGIGRIIVVNRTIERARRLAETAASYGVESSALELDR
LHEAMSAADVVLSCTGAVGAVVTLADTHRALADRDRAEFPAGDRPLVFCDLGLPRDVEPAVAGLPGVAVIDIESLQRDPA
AGAAADDTAAARSIVAEELAKYLAGQRMAEVTPTVAALRQRAAEVVEAELLRLDSRLPGLAEPERDEVARTVRRVVDKLL
HAPTVRVKQLASTPGGDSYAEALRELFELKPGAAQAVAAPMEITAIGPDRTAGLADDFTPGHRLDFEQHLGEEGKPA
>Mature_476_residues
SVLLVGVSHRSAPVSVLEKVAITDADRPKLIDKMLASSHISEAMIVSTCNRVEVYAVVDAFHGGLAEVGELLTRHSGLAM
PDLTKHAYVRYSEAAAEHLFAVASGLDSMVVGEQQVLSQIRGAYATADAQQAVGRTLHELAQHALRVGKRVHSETGIDRA
GASVVSVALDRAAQVLGDLAGRTAVVVGAGAMGGLSVAHLARAGIGRIIVVNRTIERARRLAETAASYGVESSALELDRL
HEAMSAADVVLSCTGAVGAVVTLADTHRALADRDRAEFPAGDRPLVFCDLGLPRDVEPAVAGLPGVAVIDIESLQRDPAA
GAAADDTAAARSIVAEELAKYLAGQRMAEVTPTVAALRQRAAEVVEAELLRLDSRLPGLAEPERDEVARTVRRVVDKLLH
APTVRVKQLASTPGGDSYAEALRELFELKPGAAQAVAAPMEITAIGPDRTAGLADDFTPGHRLDFEQHLGEEGKPA

Specific function: Catalyzes the NADPH-dependent reduction of glutamyl- tRNA(Glu) to glutamate 1-semialdehyde (GSA)

COG id: COG0373

COG function: function code H; Glutamyl-tRNA reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamyl-tRNA reductase family

Homologues:

Organism=Escherichia coli, GI1787461, Length=432, Percent_Identity=29.8611111111111, Blast_Score=174, Evalue=1e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM1_NOCFA (Q5YP69)

Other databases:

- EMBL:   AP006618
- RefSeq:   YP_121386.1
- HSSP:   Q9UXR8
- ProteinModelPortal:   Q5YP69
- SMR:   Q5YP69
- GeneID:   3109557
- GenomeReviews:   AP006618_GR
- KEGG:   nfa:nfa51700
- NMPDR:   fig|247156.1.peg.4968
- HOGENOM:   HBG732626
- OMA:   EVRPEHF
- ProtClustDB:   PRK00045
- BioCyc:   NFAR247156:NFA51700-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00087
- InterPro:   IPR000343
- InterPro:   IPR015896
- InterPro:   IPR015895
- InterPro:   IPR016040
- InterPro:   IPR018214
- InterPro:   IPR006151
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000445
- TIGRFAMs:   TIGR01035

Pfam domain/function: PF00745 GlutR_dimer; PF05201 GlutR_N; PF01488 Shikimate_DH; SSF69075 4pyrrol_synth_GluRdtase_C; SSF69742 GlutR

EC number: =1.2.1.70

Molecular weight: Translated: 50016; Mature: 49885

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS00747 GLUTR

Important sites: ACT_SITE 50-50 BINDING 109-109 BINDING 120-120

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVLLVGVSHRSAPVSVLEKVAITDADRPKLIDKMLASSHISEAMIVSTCNRVEVYAVVD
CCEEEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHH
AFHGGLAEVGELLTRHSGLAMPDLTKHAYVRYSEAAAEHLFAVASGLDSMVVGEQQVLSQ
HHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHH
IRGAYATADAQQAVGRTLHELAQHALRVGKRVHSETGIDRAGASVVSVALDRAAQVLGDL
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
AGRTAVVVGAGAMGGLSVAHLARAGIGRIIVVNRTIERARRLAETAASYGVESSALELDR
CCCEEEEEECCCCCHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHCCCCHHHHHHHH
LHEAMSAADVVLSCTGAVGAVVTLADTHRALADRDRAEFPAGDRPLVFCDLGLPRDVEPA
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHCCCCCCCCEEEEECCCCCCCCHH
VAGLPGVAVIDIESLQRDPAAGAAADDTAAARSIVAEELAKYLAGQRMAEVTPTVAALRQ
HHCCCCEEEEEHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
RAAEVVEAELLRLDSRLPGLAEPERDEVARTVRRVVDKLLHAPTVRVKQLASTPGGDSYA
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHH
EALRELFELKPGAAQAVAAPMEITAIGPDRTAGLADDFTPGHRLDFEQHLGEEGKPA
HHHHHHHHCCCCCHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHCCCCCCCC
>Mature Secondary Structure 
SVLLVGVSHRSAPVSVLEKVAITDADRPKLIDKMLASSHISEAMIVSTCNRVEVYAVVD
CEEEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHH
AFHGGLAEVGELLTRHSGLAMPDLTKHAYVRYSEAAAEHLFAVASGLDSMVVGEQQVLSQ
HHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHH
IRGAYATADAQQAVGRTLHELAQHALRVGKRVHSETGIDRAGASVVSVALDRAAQVLGDL
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
AGRTAVVVGAGAMGGLSVAHLARAGIGRIIVVNRTIERARRLAETAASYGVESSALELDR
CCCEEEEEECCCCCHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHCCCCHHHHHHHH
LHEAMSAADVVLSCTGAVGAVVTLADTHRALADRDRAEFPAGDRPLVFCDLGLPRDVEPA
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHCCCCCCCCEEEEECCCCCCCCHH
VAGLPGVAVIDIESLQRDPAAGAAADDTAAARSIVAEELAKYLAGQRMAEVTPTVAALRQ
HHCCCCEEEEEHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
RAAEVVEAELLRLDSRLPGLAEPERDEVARTVRRVVDKLLHAPTVRVKQLASTPGGDSYA
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHH
EALRELFELKPGAAQAVAAPMEITAIGPDRTAGLADDFTPGHRLDFEQHLGEEGKPA
HHHHHHHHCCCCCHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA