Click here to switch to the map view.

The map label for this gene is prpB [C]

Identifier: 54026881

GI number: 54026881

Start: 5122255

End: 5123007

Strand: Reverse

Name: prpB [C]

Synonym: nfa49070

Alternate gene names: 54026881

Gene position: 5123007-5122255 (Counterclockwise)

Preceding gene: 54026882

Following gene: 54026879

Centisome position: 85.08

GC content: 72.78

Gene sequence:

>753_bases
ATGACCACTCTGGAGAACAAGGCCAAGACCCTGCTCGCCCTGCACCGGCCCGGCGACCCCGTCGTGTTGCCGACGGTGTG
GGACGCGTGGTCGGCGAACGTCGCTGTCGCCGCGGGTTTCTCGGCGCTCACGATGGGCAGCCACCCGGTCGCCGACTCGC
TGGGCCGCCCCGACGGCGAGGGCATGACCTTCACCGAACTGCTCGGCCGGGTCCGCCAGGTCACCACCGCCACCGACATG
CCGGTCTCGGTCGACATCGAATCCGGCTACGGCCTCGCGCCCGCCCAGCTCATCGACGGGCTGTTGGAGGCGGGCGCGGT
CGGGCTCAACATCGAGGACACCGTGCACGGCGAGGGCCGCAGGCTGCGCGGCGCCGAGGAACACGCCGACTTCGTGCGCG
GACTGCGCGAATTCGCCGACGCCGCGGGCGTGCACGTGGTCGTCAACGCGCGCACCGACCTGTTCCTGCGCGGCGACGGC
GACGACGCCGACCGGGTGGACCGCGCGATCGAGCGGCTGAGGCTGGCCGCCGCGGCGGGCGCCGACGTGCTCTATCCGGT
CGGCAGGCACTCCGACGCCGATATGCGCAGGCTCACCGCGGAGCTGCCGTTGCCGGTCAACGCCATCGGCCTGCCCGACG
TCTCCGACAAGGCCGAGCTGGCCGCGCAGGGCGTGGCCAGGATCAGCTTCGGGCCCTTCCTGCAGGCCGCGCTGGCCAAG
GAGGCCGAGCGCCTGCTCGCCCGGTGGAAATGA

Upstream 100 bases:

>100_bases
GTCCTCGACACCCACCTCAACTGCACCACGAAACAGGCGTCCTGCTGATCCGGGCGCGTGTCAGGGTGCGGCGGGCCCGG
GCGGCGACCATGATCGAGTC

Downstream 100 bases:

>100_bases
CGTCCCACGGGCGGCGGGCACTCACTCCTTGGGTTCGACGTAGCGTGGGAAGACCGGCTCGGGCGCGGGCAGCGGGGTGC
CGGGCGTGAGCGGGGTGGCC

Product: hypothetical protein

Products: Pyruvate; Succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 250; Mature: 249

Protein sequence:

>250_residues
MTTLENKAKTLLALHRPGDPVVLPTVWDAWSANVAVAAGFSALTMGSHPVADSLGRPDGEGMTFTELLGRVRQVTTATDM
PVSVDIESGYGLAPAQLIDGLLEAGAVGLNIEDTVHGEGRRLRGAEEHADFVRGLREFADAAGVHVVVNARTDLFLRGDG
DDADRVDRAIERLRLAAAAGADVLYPVGRHSDADMRRLTAELPLPVNAIGLPDVSDKAELAAQGVARISFGPFLQAALAK
EAERLLARWK

Sequences:

>Translated_250_residues
MTTLENKAKTLLALHRPGDPVVLPTVWDAWSANVAVAAGFSALTMGSHPVADSLGRPDGEGMTFTELLGRVRQVTTATDM
PVSVDIESGYGLAPAQLIDGLLEAGAVGLNIEDTVHGEGRRLRGAEEHADFVRGLREFADAAGVHVVVNARTDLFLRGDG
DDADRVDRAIERLRLAAAAGADVLYPVGRHSDADMRRLTAELPLPVNAIGLPDVSDKAELAAQGVARISFGPFLQAALAK
EAERLLARWK
>Mature_249_residues
TTLENKAKTLLALHRPGDPVVLPTVWDAWSANVAVAAGFSALTMGSHPVADSLGRPDGEGMTFTELLGRVRQVTTATDMP
VSVDIESGYGLAPAQLIDGLLEAGAVGLNIEDTVHGEGRRLRGAEEHADFVRGLREFADAAGVHVVVNARTDLFLRGDGD
DADRVDRAIERLRLAAAAGADVLYPVGRHSDADMRRLTAELPLPVNAIGLPDVSDKAELAAQGVARISFGPFLQAALAKE
AERLLARWK

Specific function: Catalyzes The Formation Of Pyruvate And Succinate From 2-Methylisocitrate (By Similarity). [C]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786525, Length=219, Percent_Identity=27.8538812785388, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR015813 [H]

Pfam domain/function: NA

EC number: 4.1.3.30 [C]

Molecular weight: Translated: 26528; Mature: 26397

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLENKAKTLLALHRPGDPVVLPTVWDAWSANVAVAAGFSALTMGSHPVADSLGRPDGE
CCCCHHHHHEEEEEECCCCCEEECCCCCCCCCCEEEECCCHHEECCCCCCHHHCCCCCCC
GMTFTELLGRVRQVTTATDMPVSVDIESGYGLAPAQLIDGLLEAGAVGLNIEDTVHGEGR
CCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCC
RLRGAEEHADFVRGLREFADAAGVHVVVNARTDLFLRGDGDDADRVDRAIERLRLAAAAG
CCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHCC
ADVLYPVGRHSDADMRRLTAELPLPVNAIGLPDVSDKAELAAQGVARISFGPFLQAALAK
CCEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHH
EAERLLARWK
HHHHHHHCCC
>Mature Secondary Structure 
TTLENKAKTLLALHRPGDPVVLPTVWDAWSANVAVAAGFSALTMGSHPVADSLGRPDGE
CCCHHHHHEEEEEECCCCCEEECCCCCCCCCCEEEECCCHHEECCCCCCHHHCCCCCCC
GMTFTELLGRVRQVTTATDMPVSVDIESGYGLAPAQLIDGLLEAGAVGLNIEDTVHGEGR
CCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCC
RLRGAEEHADFVRGLREFADAAGVHVVVNARTDLFLRGDGDDADRVDRAIERLRLAAAAG
CCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHCC
ADVLYPVGRHSDADMRRLTAELPLPVNAIGLPDVSDKAELAAQGVARISFGPFLQAALAK
CCEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHH
EAERLLARWK
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: methylisocitrate [C]

Specific reaction: methylisocitrate <==> Pyruvate + Succinate [C]

General reaction: Elimination [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]