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The map label for this gene is eno

Identifier: 54026833

GI number: 54026833

Start: 5070818

End: 5072104

Strand: Reverse

Name: eno

Synonym: nfa48590

Alternate gene names: 54026833

Gene position: 5072104-5070818 (Counterclockwise)

Preceding gene: 54026834

Following gene: 54026832

Centisome position: 84.24

GC content: 70.55

Gene sequence:

>1287_bases
GTGGCCATCATCGAACAGGTCGGAGCGCGCGAAATCCTGGATTCCCGCGGAAACCCCACCGTCGAGGTCGAGATCGCCCT
CGACGACGGCACCCTCACCCGGGCCGCCGTGCCGTCGGGTGCCTCCACCGGTGAGCACGAGGCCGTCGAACTGCGCGACG
GGGGCGACCGCTACAACGGCAAGGGTGTGCTCAAGGCCGTCGAGGGCGTCCTCGACGAGATCGCGCCCGCGGTGATCGGC
CTGGACGCGGTCGAGCAGCGCACCGTCGACCAGGTGCTGCTGGACCTGGACGGCACCCCCGACAAGTCCCGCCTCGGCGC
CAACGCCCTGCTCGGCGTCTCGCTCGCGGTGGCGCGCGCGGCCGCGGAGTCCTCGGGCCTCGAGCTGTTCCGCTACCTGG
GCGGCCCGAACGCGCACGTGCTTCCGGTGCCGATGATGAACATCCTCAACGGCGGCGCGCACGCCGACACCGGGGTGGAC
GTGCAGGAGTTCATGGTCGCCCCGATCGGCGCGCCCACCTTCAAGGAGTCGCTGCGCTGGGGCGCGGAGGTCTACCACGC
GCTCAAGGCGGTGCTGAAGTCCAAGGGCCTGTCCACCGGCCTCGGTGACGAGGGCGGCTTCGCCCCCGACGTGGCGGGCA
CCCGCGAGGCGCTGGACCTGATCGCCCAGGCCATCGCCAAGACCGGCCTGAAGCTGGGCAGTGACGTCGCGCTCGCGCTG
GACGTCGCGGCCACCGAGTTCTACACCTCCGGCAGCGGCTACAAGTTCGAGGGCACCGTCCGCTCGGCCGAGGAGATGGC
GCAGTTCTACAGCGAACTGCTCGGCGCCTACCCGCTGGTCTCCATCGAGGACCCGCTCTCGGAGGACGACTGGGACGGCT
GGGTCGCGCTCACCGACCAGATCGGCGACAAGATCCAGCTGGTCGGCGACGACCTGTTCGTCACCAACCCCGAGCGGCTC
GAGGACGGCATCGCCAAGGGCGCGGCCAACGCGCTGCTGGTGAAGGTCAACCAGATCGGCACCCTCACCGAGACCCTCGA
CGCCGTCGAGCTCGCGCACCGCAACGGCTACAAGACGATGATGTCGCACCGCTCGGGCGAGACCGAGGACACCACCATCG
CCGACCTGGCCGTCGCCGTGGGCAGCGGCCAGATCAAGACCGGCGCCCCCGCGCGCAGCGAGCGTGTCGCCAAGTACAAC
CAGCTGCTGCGCATCGAGGACGCGCTCGGCGATTCCGCGCGGTACGCCGGCGACGTGGCGTTCCCGCGCTTCGTGTTCGA
CGGGTAA

Upstream 100 bases:

>100_bases
GAGTGCCGGTTACCTCGGACGCGGCGGGGACGCTAGGCTCGGCACCGCACGGCCCAACCGTCAGACCCACCGTGCCAGCA
GCCGAAGGAGAGTCGTTTTC

Downstream 100 bases:

>100_bases
GGCTCGTGTTCGAAGGGTGAGGCGAGGAGATCCATGACGGAGCGACGCGCGCGCGGTACCAGTCCGGCAGGACGCGGTGA
CCGCCGCACGTCGCGCGCGG

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MAIIEQVGAREILDSRGNPTVEVEIALDDGTLTRAAVPSGASTGEHEAVELRDGGDRYNGKGVLKAVEGVLDEIAPAVIG
LDAVEQRTVDQVLLDLDGTPDKSRLGANALLGVSLAVARAAAESSGLELFRYLGGPNAHVLPVPMMNILNGGAHADTGVD
VQEFMVAPIGAPTFKESLRWGAEVYHALKAVLKSKGLSTGLGDEGGFAPDVAGTREALDLIAQAIAKTGLKLGSDVALAL
DVAATEFYTSGSGYKFEGTVRSAEEMAQFYSELLGAYPLVSIEDPLSEDDWDGWVALTDQIGDKIQLVGDDLFVTNPERL
EDGIAKGAANALLVKVNQIGTLTETLDAVELAHRNGYKTMMSHRSGETEDTTIADLAVAVGSGQIKTGAPARSERVAKYN
QLLRIEDALGDSARYAGDVAFPRFVFDG

Sequences:

>Translated_428_residues
MAIIEQVGAREILDSRGNPTVEVEIALDDGTLTRAAVPSGASTGEHEAVELRDGGDRYNGKGVLKAVEGVLDEIAPAVIG
LDAVEQRTVDQVLLDLDGTPDKSRLGANALLGVSLAVARAAAESSGLELFRYLGGPNAHVLPVPMMNILNGGAHADTGVD
VQEFMVAPIGAPTFKESLRWGAEVYHALKAVLKSKGLSTGLGDEGGFAPDVAGTREALDLIAQAIAKTGLKLGSDVALAL
DVAATEFYTSGSGYKFEGTVRSAEEMAQFYSELLGAYPLVSIEDPLSEDDWDGWVALTDQIGDKIQLVGDDLFVTNPERL
EDGIAKGAANALLVKVNQIGTLTETLDAVELAHRNGYKTMMSHRSGETEDTTIADLAVAVGSGQIKTGAPARSERVAKYN
QLLRIEDALGDSARYAGDVAFPRFVFDG
>Mature_427_residues
AIIEQVGAREILDSRGNPTVEVEIALDDGTLTRAAVPSGASTGEHEAVELRDGGDRYNGKGVLKAVEGVLDEIAPAVIGL
DAVEQRTVDQVLLDLDGTPDKSRLGANALLGVSLAVARAAAESSGLELFRYLGGPNAHVLPVPMMNILNGGAHADTGVDV
QEFMVAPIGAPTFKESLRWGAEVYHALKAVLKSKGLSTGLGDEGGFAPDVAGTREALDLIAQAIAKTGLKLGSDVALALD
VAATEFYTSGSGYKFEGTVRSAEEMAQFYSELLGAYPLVSIEDPLSEDDWDGWVALTDQIGDKIQLVGDDLFVTNPERLE
DGIAKGAANALLVKVNQIGTLTETLDAVELAHRNGYKTMMSHRSGETEDTTIADLAVAVGSGQIKTGAPARSERVAKYNQ
LLRIEDALGDSARYAGDVAFPRFVFDG

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=431, Percent_Identity=53.1322505800464, Blast_Score=433, Evalue=1e-121,
Organism=Homo sapiens, GI301897477, Length=432, Percent_Identity=51.8518518518518, Blast_Score=426, Evalue=1e-119,
Organism=Homo sapiens, GI301897469, Length=432, Percent_Identity=51.8518518518518, Blast_Score=426, Evalue=1e-119,
Organism=Homo sapiens, GI4503571, Length=434, Percent_Identity=51.1520737327189, Blast_Score=421, Evalue=1e-118,
Organism=Homo sapiens, GI301897479, Length=430, Percent_Identity=47.4418604651163, Blast_Score=369, Evalue=1e-102,
Organism=Homo sapiens, GI169201331, Length=335, Percent_Identity=24.4776119402985, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI169201757, Length=335, Percent_Identity=24.4776119402985, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI239744207, Length=335, Percent_Identity=24.4776119402985, Blast_Score=107, Evalue=3e-23,
Organism=Escherichia coli, GI1789141, Length=425, Percent_Identity=55.7647058823529, Blast_Score=456, Evalue=1e-129,
Organism=Caenorhabditis elegans, GI71995829, Length=429, Percent_Identity=51.7482517482517, Blast_Score=415, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI17536383, Length=429, Percent_Identity=51.7482517482517, Blast_Score=414, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI32563855, Length=189, Percent_Identity=51.3227513227513, Blast_Score=194, Evalue=6e-50,
Organism=Saccharomyces cerevisiae, GI6321693, Length=432, Percent_Identity=51.3888888888889, Blast_Score=395, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6323985, Length=434, Percent_Identity=49.3087557603687, Blast_Score=387, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6324974, Length=434, Percent_Identity=49.3087557603687, Blast_Score=387, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6324969, Length=434, Percent_Identity=49.3087557603687, Blast_Score=387, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6321968, Length=435, Percent_Identity=50.8045977011494, Blast_Score=370, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24580918, Length=429, Percent_Identity=51.7482517482517, Blast_Score=407, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580916, Length=429, Percent_Identity=51.7482517482517, Blast_Score=407, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580920, Length=429, Percent_Identity=51.7482517482517, Blast_Score=407, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580914, Length=429, Percent_Identity=51.7482517482517, Blast_Score=407, Evalue=1e-114,
Organism=Drosophila melanogaster, GI281360527, Length=429, Percent_Identity=51.7482517482517, Blast_Score=406, Evalue=1e-113,
Organism=Drosophila melanogaster, GI17137654, Length=429, Percent_Identity=51.7482517482517, Blast_Score=406, Evalue=1e-113,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_NOCFA (Q5YQ30)

Other databases:

- EMBL:   AP006618
- RefSeq:   YP_121075.1
- ProteinModelPortal:   Q5YQ30
- SMR:   Q5YQ30
- GeneID:   3109727
- GenomeReviews:   AP006618_GR
- KEGG:   nfa:nfa48590
- NMPDR:   fig|247156.1.peg.4675
- HOGENOM:   HBG726599
- OMA:   GSHADSN
- ProtClustDB:   PRK00077
- BioCyc:   NFAR247156:NFA48590-MONOMER
- BRENDA:   4.2.1.11
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 44994; Mature: 44863

Theoretical pI: Translated: 4.25; Mature: 4.25

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 204-204 ACT_SITE 335-335 BINDING 154-154 BINDING 163-163 BINDING 283-283 BINDING 310-310 BINDING 335-335 BINDING 386-386

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIIEQVGAREILDSRGNPTVEVEIALDDGTLTRAAVPSGASTGEHEAVELRDGGDRYNG
CCHHHHCCHHHHHHCCCCCEEEEEEEECCCCEEEEECCCCCCCCCCCEEEECCCCCCCCC
KGVLKAVEGVLDEIAPAVIGLDAVEQRTVDQVLLDLDGTPDKSRLGANALLGVSLAVARA
CHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHH
AAESSGLELFRYLGGPNAHVLPVPMMNILNGGAHADTGVDVQEFMVAPIGAPTFKESLRW
HHHHCHHHHHHHHCCCCCEEECCCHHHHHCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHH
GAEVYHALKAVLKSKGLSTGLGDEGGFAPDVAGTREALDLIAQAIAKTGLKLGSDVALAL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEE
DVAATEFYTSGSGYKFEGTVRSAEEMAQFYSELLGAYPLVSIEDPLSEDDWDGWVALTDQ
EHHHHHHEECCCCEEEECHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCEEEEECC
IGDKIQLVGDDLFVTNPERLEDGIAKGAANALLVKVNQIGTLTETLDAVELAHRNGYKTM
CCCEEEEECCCEEECCHHHHHHHHHHCCCCEEEEEEHHCCCHHHHHHHHHHHHCCCHHHH
MSHRSGETEDTTIADLAVAVGSGQIKTGAPARSERVAKYNQLLRIEDALGDSARYAGDVA
HHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHCCCCC
FPRFVFDG
CCCCCCCC
>Mature Secondary Structure 
AIIEQVGAREILDSRGNPTVEVEIALDDGTLTRAAVPSGASTGEHEAVELRDGGDRYNG
CHHHHCCHHHHHHCCCCCEEEEEEEECCCCEEEEECCCCCCCCCCCEEEECCCCCCCCC
KGVLKAVEGVLDEIAPAVIGLDAVEQRTVDQVLLDLDGTPDKSRLGANALLGVSLAVARA
CHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHH
AAESSGLELFRYLGGPNAHVLPVPMMNILNGGAHADTGVDVQEFMVAPIGAPTFKESLRW
HHHHCHHHHHHHHCCCCCEEECCCHHHHHCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHH
GAEVYHALKAVLKSKGLSTGLGDEGGFAPDVAGTREALDLIAQAIAKTGLKLGSDVALAL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEE
DVAATEFYTSGSGYKFEGTVRSAEEMAQFYSELLGAYPLVSIEDPLSEDDWDGWVALTDQ
EHHHHHHEECCCCEEEECHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCEEEEECC
IGDKIQLVGDDLFVTNPERLEDGIAKGAANALLVKVNQIGTLTETLDAVELAHRNGYKTM
CCCEEEEECCCEEECCHHHHHHHHHHCCCCEEEEEEHHCCCHHHHHHHHHHHHCCCHHHH
MSHRSGETEDTTIADLAVAVGSGQIKTGAPARSERVAKYNQLLRIEDALGDSARYAGDVA
HHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHCCCCC
FPRFVFDG
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA