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The map label for this gene is cofE

Identifier: 54026593

GI number: 54026593

Start: 4806907

End: 4808265

Strand: Reverse

Name: cofE

Synonym: nfa46200

Alternate gene names: 54026593

Gene position: 4808265-4806907 (Counterclockwise)

Preceding gene: 54026594

Following gene: 54026592

Centisome position: 79.86

GC content: 74.39

Gene sequence:

>1359_bases
GTGGTGAGCGCGCCCGGCGACCACGCGGGCGTGAGTCGCGAGGGGTTGCGGATCCTGCCCGTCACCGGCCTCCCCGAGTT
CCGTCCCGGCGACGACGTGGCCGAGCGGATCGCCGCCGCCGCGCCCTGGCTGGCCGACGGCGACATCCTCGTGGTCACCA
GCAAGATCATCGCCAAGGCCGAGGGCAGGGTCGTGCCCGCGCCGGTGGACCCCGAGGAACGCGACGCCGTGCGCCGGGCG
CTGGTCGAGCAGGAGGCCGTGCGGGTGCTGGCGCGCAAGGGCCGCACCCTCATCACCGAGAACAAGCTCGGCATCGTGCA
GGCGGCCTCCGGTGTCGACGGATCCAACGTCGAGAAGGACGAATTGGTGCTGCTGCCCGCCGATCCCGACGCCAGCGCCG
CGGCGCTGCGCGCGGGCCTGGCCGAGCGGCTCGGCGTGCGGGTGGCGGTGGTCGTCACCGACACGATGGGCCGGGCCTGG
CGCAACGGCCAGACCGACGCCGCGATCGGCGCCGCCGGCCTGCGCGTGCTGCACGACTATGCCGGGGCGGTCGACGGACA
GGGCAACGAACTGCACGTCACCCAGGTCGCCGTCGCCGACGAACTGGCCGCCGCCGCCGACCTGGTGAAGGGCAAGTTGC
GCGGCGTTCCGGTGGCCGTGGTGCGCGGACTGCCCACGCAGGAGGACGGTTCCACCGCCGCCGATCTGGTGCGCGCCGGC
GAGGAGGACCTGTTCTGGCTGGGCACCGCCGAGGCCGTCGAGCGCGGCAGGCGCGAAGCGGTGCTGCTGCGCCGCTCGGT
GCGGACCTTCGCCGACACCCCGGTCGAGCCCGACACGATCCGCGCCGCCGTGTCGGTCGCACTGACCGCGCCCGCCCCGC
ACCACACCCGCCCGGTGCGGTTCGTGTGGGTGCGCGACGCGCAGCGACGGCAGCGGCTGCTCGCCGCGATGGCCGACAAG
TGGCGTGCCGACCTGCGCGCCGACGGACTCGACCCGGAACGGATCGAACGGCGGGTCGGGCGCGGCCGCATCCTGTTCGA
CGCCCCCGAGGTGCTCATCCCGTGCTGCGTGCCCGACGGCGCGCACTCCTACCCGGACGAGCGCAGGCAGGCGGCCGAAA
CCACCATGTTCACCGTCGCGGTCGGCGCCGCGGTGCAGGGACTGCTCGTCGCACTCGCCACCGAGGGCGTGGGCAGCTGC
TGGATCGGATCGACCATCTTCGCCCCCGAGGTCACCCGCGCCGAGCTGGATCTTCCCGCGGACTGGAACCCCTTGGGCGC
CATCGCGGTCGGCTACCCGACCGAAGAGCTCGCACCTCGTCCGCCGCGCGATCCCGGCGACGGGCTGGTCGAACGGTGA

Upstream 100 bases:

>100_bases
AGGTGCCCGGCGTCGAGGTGCGCAGCGTGCCGCTGCTCATGACCGACCCGCCGACCACCGCCGAAATCGTCCGGGAGGCC
CTGGATCTCGCGGGGGTGAA

Downstream 100 bases:

>100_bases
GCGCGGAATCGCTGCACGCCTCCGCCACCGCCCTGCTGGAGGGCTGGTCGCCGCCGACGGGACCCGAGCGGTCGGTGCGC
GAGGCGATGCTCGCCTTTCT

Product: F420-0--gamma-glutamyl ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MVSAPGDHAGVSREGLRILPVTGLPEFRPGDDVAERIAAAAPWLADGDILVVTSKIIAKAEGRVVPAPVDPEERDAVRRA
LVEQEAVRVLARKGRTLITENKLGIVQAASGVDGSNVEKDELVLLPADPDASAAALRAGLAERLGVRVAVVVTDTMGRAW
RNGQTDAAIGAAGLRVLHDYAGAVDGQGNELHVTQVAVADELAAAADLVKGKLRGVPVAVVRGLPTQEDGSTAADLVRAG
EEDLFWLGTAEAVERGRREAVLLRRSVRTFADTPVEPDTIRAAVSVALTAPAPHHTRPVRFVWVRDAQRRQRLLAAMADK
WRADLRADGLDPERIERRVGRGRILFDAPEVLIPCCVPDGAHSYPDERRQAAETTMFTVAVGAAVQGLLVALATEGVGSC
WIGSTIFAPEVTRAELDLPADWNPLGAIAVGYPTEELAPRPPRDPGDGLVER

Sequences:

>Translated_452_residues
MVSAPGDHAGVSREGLRILPVTGLPEFRPGDDVAERIAAAAPWLADGDILVVTSKIIAKAEGRVVPAPVDPEERDAVRRA
LVEQEAVRVLARKGRTLITENKLGIVQAASGVDGSNVEKDELVLLPADPDASAAALRAGLAERLGVRVAVVVTDTMGRAW
RNGQTDAAIGAAGLRVLHDYAGAVDGQGNELHVTQVAVADELAAAADLVKGKLRGVPVAVVRGLPTQEDGSTAADLVRAG
EEDLFWLGTAEAVERGRREAVLLRRSVRTFADTPVEPDTIRAAVSVALTAPAPHHTRPVRFVWVRDAQRRQRLLAAMADK
WRADLRADGLDPERIERRVGRGRILFDAPEVLIPCCVPDGAHSYPDERRQAAETTMFTVAVGAAVQGLLVALATEGVGSC
WIGSTIFAPEVTRAELDLPADWNPLGAIAVGYPTEELAPRPPRDPGDGLVER
>Mature_452_residues
MVSAPGDHAGVSREGLRILPVTGLPEFRPGDDVAERIAAAAPWLADGDILVVTSKIIAKAEGRVVPAPVDPEERDAVRRA
LVEQEAVRVLARKGRTLITENKLGIVQAASGVDGSNVEKDELVLLPADPDASAAALRAGLAERLGVRVAVVVTDTMGRAW
RNGQTDAAIGAAGLRVLHDYAGAVDGQGNELHVTQVAVADELAAAADLVKGKLRGVPVAVVRGLPTQEDGSTAADLVRAG
EEDLFWLGTAEAVERGRREAVLLRRSVRTFADTPVEPDTIRAAVSVALTAPAPHHTRPVRFVWVRDAQRRQRLLAAMADK
WRADLRADGLDPERIERRVGRGRILFDAPEVLIPCCVPDGAHSYPDERRQAAETTMFTVAVGAAVQGLLVALATEGVGSC
WIGSTIFAPEVTRAELDLPADWNPLGAIAVGYPTEELAPRPPRDPGDGLVER

Specific function: Catalyzes the GTP-dependent successive addition of multiple gamma-linked L-glutamates to the L-lactyl phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (F420-0) to form polyglutamated F420 derivatives

COG id: COG1478

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the CofE family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COFE_NOCFA (Q5YQS0)

Other databases:

- EMBL:   AP006618
- RefSeq:   YP_120835.1
- ProteinModelPortal:   Q5YQS0
- SMR:   Q5YQS0
- GeneID:   3109078
- GenomeReviews:   AP006618_GR
- KEGG:   nfa:nfa46200
- NMPDR:   fig|247156.1.peg.4445
- HOGENOM:   HBG578188
- OMA:   PAPHHTT
- ProtClustDB:   PRK13294
- BioCyc:   NFAR247156:NFA46200-MONOMER
- HAMAP:   MF_01259
- InterPro:   IPR002847
- InterPro:   IPR008225
- InterPro:   IPR019943
- InterPro:   IPR000415
- TIGRFAMs:   TIGR03553
- TIGRFAMs:   TIGR01916

Pfam domain/function: PF01996 F420_ligase; PF00881 Nitroreductase; SSF55469 Nitroreductase

EC number: NA

Molecular weight: Translated: 47993; Mature: 47993

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSAPGDHAGVSREGLRILPVTGLPEFRPGDDVAERIAAAAPWLADGDILVVTSKIIAKA
CCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEHHHHHHC
EGRVVPAPVDPEERDAVRRALVEQEAVRVLARKGRTLITENKLGIVQAASGVDGSNVEKD
CCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEECCCCCCCCCCCC
ELVLLPADPDASAAALRAGLAERLGVRVAVVVTDTMGRAWRNGQTDAAIGAAGLRVLHDY
CEEEEECCCCHHHHHHHHHHHHHHCCEEEEEEECCHHHHHCCCCCCHHHHHHHHHHHHHH
AGAVDGQGNELHVTQVAVADELAAAADLVKGKLRGVPVAVVRGLPTQEDGSTAADLVRAG
HCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCHHHHHHHCC
EEDLFWLGTAEAVERGRREAVLLRRSVRTFADTPVEPDTIRAAVSVALTAPAPHHTRPVR
CCCEEEEECHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHEEEEEEECCCCCCCCCEE
FVWVRDAQRRQRLLAAMADKWRADLRADGLDPERIERRVGRGRILFDAPEVLIPCCVPDG
EEEECCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEEECCCEEEEECCCCC
AHSYPDERRQAAETTMFTVAVGAAVQGLLVALATEGVGSCWIGSTIFAPEVTRAELDLPA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCEECCCCCCCEEECCCC
DWNPLGAIAVGYPTEELAPRPPRDPGDGLVER
CCCCCCEEEECCCHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MVSAPGDHAGVSREGLRILPVTGLPEFRPGDDVAERIAAAAPWLADGDILVVTSKIIAKA
CCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEHHHHHHC
EGRVVPAPVDPEERDAVRRALVEQEAVRVLARKGRTLITENKLGIVQAASGVDGSNVEKD
CCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEECCCCCCCCCCCC
ELVLLPADPDASAAALRAGLAERLGVRVAVVVTDTMGRAWRNGQTDAAIGAAGLRVLHDY
CEEEEECCCCHHHHHHHHHHHHHHCCEEEEEEECCHHHHHCCCCCCHHHHHHHHHHHHHH
AGAVDGQGNELHVTQVAVADELAAAADLVKGKLRGVPVAVVRGLPTQEDGSTAADLVRAG
HCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCHHHHHHHCC
EEDLFWLGTAEAVERGRREAVLLRRSVRTFADTPVEPDTIRAAVSVALTAPAPHHTRPVR
CCCEEEEECHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHEEEEEEECCCCCCCCCEE
FVWVRDAQRRQRLLAAMADKWRADLRADGLDPERIERRVGRGRILFDAPEVLIPCCVPDG
EEEECCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEEECCCEEEEECCCCC
AHSYPDERRQAAETTMFTVAVGAAVQGLLVALATEGVGSCWIGSTIFAPEVTRAELDLPA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCEECCCCCCCEEECCCC
DWNPLGAIAVGYPTEELAPRPPRDPGDGLVER
CCCCCCEEEECCCHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA