Click here to switch to the map view.

The map label for this gene is entD [C]

Identifier: 54026024

GI number: 54026024

Start: 4200539

End: 4201207

Strand: Reverse

Name: entD [C]

Synonym: nfa40540

Alternate gene names: 54026024

Gene position: 4201207-4200539 (Counterclockwise)

Preceding gene: 54026025

Following gene: 54026023

Centisome position: 69.77

GC content: 70.85

Gene sequence:

>669_bases
ATGATCGAGAACATTCTGCCCAGCGGGGTGGCCGCTGCCGAGCTGCTGGAGTACCCGGAGGATCTGAAGCCGCACCCGGC
CGAGGAACATCTGATCGCCCAGTCGGTGGAGAAGCGCCGTCGCGACTTCATCGGTGCCAGGCACTGCGCGCGCCTGGCGC
TGCGTGAACTCGGCGAGCCGCCGGTGGCGATCGGCAAGGGGGAGCGGGGCGCGCCGGTGTGGCCGCGCGGCATCGTCGGC
AGCCTCACCCACTGCGACGGCTACCGGGCCGCGGCGCTGGCGCACAAGATCCGGTTCCGTTCGGTCGGTATCGACGCCGA
GCCGCACGGCCCGCTGCCCGACGGCGTGCTCGACTCGGTGAGCCTGCCGCAGGAGCGGGAGTGGCTGCGCCGCACGGATT
CCGGCCTGCACCTGGACCGGCTGCTGTTCTGCGCCAAGGAAGCCACCTACAAGGCATGGTTCCCGCTCACCGCGCGCTGG
CTCGGCTTCGAGGACGCGCACATCACCTTCACCGTCGAGGAGGACGGCGCGGGCGGCGGTTCGGGCACCTTCCACACCGA
TCTGCTGGTGCCGGGCCAGACCACCGACGGTGGCCTGCCGCTGACCTCGTTCGACGGACGCTGGCTGATCGCCGACGGGC
TGATCCTGACCGCGATCGTGCACGACTGA

Upstream 100 bases:

>100_bases
CACAGCGTAGGCGCGGACTGTGAGGCGGCCGTCGAATGTCGGGAAGCGCTCGGTTCGCCCGCGGGGGAGGGTCGCCCGGC
TTCGGCTAGGCTCGCAACCG

Downstream 100 bases:

>100_bases
TGGGCGAGCGCAGCGCACCGACGCGCCGCGTCGACCCGCTCGGCGGGCTGCTCGTCGTCGACAAGGACGGCGGCATGACC
AGCCACGACGTCGTCGCGCG

Product: putative phosphopantetheinyl transferase

Products: pyrophosphate; AMP; enterobactin; aryl carrier protein/isochorismatase; 3',5'-ADP; serine activating enzyme + [C]

Alternate protein names: Phosphopantetheinyl Transferase; Phosphopantetheinyl Transferase PptA; Sfp-Type Phosphopantetheinyl Transferase; Holo-[Acyl-Carrier-Protein] Synthase; EntD Family Protein; 4-Phosphopantetheinyl Tranferase; Non-Ribosomal Peptide Synthetase; 4-Phosphopantetheinyl Transferase Superfamily Protein; Phosphopantetheinyl Transferase PptII; Siderophore Biosynthesis Protein; Transferase; Isochorismatase EntB; 4-Phosphopantetheinyl Transferase EntD; 4-Phosphopantetheinyl Transferase Superfamily; Vibriobactin Synthase Component D

Number of amino acids: Translated: 222; Mature: 222

Protein sequence:

>222_residues
MIENILPSGVAAAELLEYPEDLKPHPAEEHLIAQSVEKRRRDFIGARHCARLALRELGEPPVAIGKGERGAPVWPRGIVG
SLTHCDGYRAAALAHKIRFRSVGIDAEPHGPLPDGVLDSVSLPQEREWLRRTDSGLHLDRLLFCAKEATYKAWFPLTARW
LGFEDAHITFTVEEDGAGGGSGTFHTDLLVPGQTTDGGLPLTSFDGRWLIADGLILTAIVHD

Sequences:

>Translated_222_residues
MIENILPSGVAAAELLEYPEDLKPHPAEEHLIAQSVEKRRRDFIGARHCARLALRELGEPPVAIGKGERGAPVWPRGIVG
SLTHCDGYRAAALAHKIRFRSVGIDAEPHGPLPDGVLDSVSLPQEREWLRRTDSGLHLDRLLFCAKEATYKAWFPLTARW
LGFEDAHITFTVEEDGAGGGSGTFHTDLLVPGQTTDGGLPLTSFDGRWLIADGLILTAIVHD
>Mature_222_residues
MIENILPSGVAAAELLEYPEDLKPHPAEEHLIAQSVEKRRRDFIGARHCARLALRELGEPPVAIGKGERGAPVWPRGIVG
SLTHCDGYRAAALAHKIRFRSVGIDAEPHGPLPDGVLDSVSLPQEREWLRRTDSGLHLDRLLFCAKEATYKAWFPLTARW
LGFEDAHITFTVEEDGAGGGSGTFHTDLLVPGQTTDGGLPLTSFDGRWLIADGLILTAIVHD

Specific function: Catalyzes The Transfer Of The 4'-Phosphopantetheine Moiety From Coenzyme A To Apo-Domains Of Both Entb (An Arcp Domain) And Entf (A Pcp Domain). Plays An Essential Role In The Assembly Of The Enterobactin. [C]

COG id: COG2977

COG function: function code Q; Phosphopantetheinyl transferase component of siderophore synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.7.8.- [C]

Molecular weight: Translated: 24234; Mature: 24234

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIENILPSGVAAAELLEYPEDLKPHPAEEHLIAQSVEKRRRDFIGARHCARLALRELGEP
CCCCCCCCCHHHHHHHHCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PVAIGKGERGAPVWPRGIVGSLTHCDGYRAAALAHKIRFRSVGIDAEPHGPLPDGVLDSV
CEEECCCCCCCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHEECCCCCCCCCCCCCHHHCC
SLPQEREWLRRTDSGLHLDRLLFCAKEATYKAWFPLTARWLGFEDAHITFTVEEDGAGGG
CCCHHHHHHHHCCCCCCHHHHHHHHHHCCCEEECCCCHHCCCCCCCEEEEEEECCCCCCC
SGTFHTDLLVPGQTTDGGLPLTSFDGRWLIADGLILTAIVHD
CCCEEEEEEECCCCCCCCCCCCCCCCCEEEECCEEEEEEECC
>Mature Secondary Structure
MIENILPSGVAAAELLEYPEDLKPHPAEEHLIAQSVEKRRRDFIGARHCARLALRELGEP
CCCCCCCCCHHHHHHHHCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PVAIGKGERGAPVWPRGIVGSLTHCDGYRAAALAHKIRFRSVGIDAEPHGPLPDGVLDSV
CEEECCCCCCCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHEECCCCCCCCCCCCCHHHCC
SLPQEREWLRRTDSGLHLDRLLFCAKEATYKAWFPLTARWLGFEDAHITFTVEEDGAGGG
CCCHHHHHHHHCCCCCCHHHHHHHHHHCCCEEECCCCHHCCCCCCCEEEEEEECCCCCCC
SGTFHTDLLVPGQTTDGGLPLTSFDGRWLIADGLILTAIVHD
CCCEEEEEEECCCCCCCCCCCCCCCCCEEEECCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 6 ATP; L-serine; 2,3-dihydroxybenzoate; Coenzyme A; apo-EntB; apo-serine activating enzyme [C]

Specific reaction: 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin Coenzyme A + apo-EntB = aryl carrier protein/isochorismatase + 3',5'-ADP Coenzyme A + apo-serine activating enzyme = 3',5'-ADP + serine activating enzyme [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA