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The map label for this gene is truB

Identifier: 54026023

GI number: 54026023

Start: 4199619

End: 4200539

Strand: Reverse

Name: truB

Synonym: nfa40530

Alternate gene names: 54026023

Gene position: 4200539-4199619 (Counterclockwise)

Preceding gene: 54026024

Following gene: 54026019

Centisome position: 69.76

GC content: 73.83

Gene sequence:

>921_bases
ATGGGCGAGCGCAGCGCACCGACGCGCCGCGTCGACCCGCTCGGCGGGCTGCTCGTCGTCGACAAGGACGGCGGCATGAC
CAGCCACGACGTCGTCGCGCGCTGCCGGAAGATCCTGGGCACCAGGAAGATCGGGCACGCGGGCACCCTCGACCCGATGG
CCACCGGCGTGCTGGTGCTCGGCGTGGAGCGGGCCACCAAGCTGCTCGGGTTGCTCACCCTCACCACCAAGGCCTACACC
GCGACCATCCGCCTCGGCAGTGCCACCACCACCGACGACGCCGAGGGCGAGGTGCTCACCACCGTCCCGGCGGGCCACCT
CGGCGACGCCGAGGTCGCCGCGGGGGTGGCGGCGCTGACCGGCGACATCCAGCAGGTGCCCGCCACCGTCAGCGCGATCA
AGATCGGCGGCGAGCGCGCGTACGCCCGCCATCGCGCGGGGGAGCAGGTCGAGTTGGCCGCGCGGCCGGTGACGGTGTCG
CGCTTCGAGGTCCTGGCTCGCCGCGACGTCGCCGGATTCGTCGACCTCGACGTGGTGGTGGAGTGCTCCTCGGGTACCTA
CGTGCGGGCGCTGGCCCGAGATCTGGGTGCGGCGCTCGGTGTCGGCGGCCACTTGACGGCGCTGCGCCGCACCCGGGTGG
GCCCGTTCACCCTCGACCACGCCCGCACGCTCGACGCGCTCGCCGACGAGCCTCGGCTGAACCTCGACATGGACGAGGCC
GTGCGAATCGCCTTCCCGCACCGGGCGATCGACGCCCGCGAGGCGGAGTCGCTGCGCGATGGCCGCTGGCTCGACCCGGT
CGGCATCCCCGGCGTGTACGCCGCCCTGACCGCCGACGGCACCGCCATCGCGCTGCTCGAGGAGAAGGGCAAGCGCGCCT
CGCCGGTATTCGTGGTGCGCCCACGCGGTCTGGTGGACTGA

Upstream 100 bases:

>100_bases
TGCCGGGCCAGACCACCGACGGTGGCCTGCCGCTGACCTCGTTCGACGGACGCTGGCTGATCGCCGACGGGCTGATCCTG
ACCGCGATCGTGCACGACTG

Downstream 100 bases:

>100_bases
CCGCGCCGGTGCGTGCGCCGGATCTGCTCGGCGGCTGGGCCCTACGGGCCAGGTGGCCGCGCCGGCCCCGTGGCTGTGGC
CGATGGGCCCGGTCGCTGCG

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MGERSAPTRRVDPLGGLLVVDKDGGMTSHDVVARCRKILGTRKIGHAGTLDPMATGVLVLGVERATKLLGLLTLTTKAYT
ATIRLGSATTTDDAEGEVLTTVPAGHLGDAEVAAGVAALTGDIQQVPATVSAIKIGGERAYARHRAGEQVELAARPVTVS
RFEVLARRDVAGFVDLDVVVECSSGTYVRALARDLGAALGVGGHLTALRRTRVGPFTLDHARTLDALADEPRLNLDMDEA
VRIAFPHRAIDAREAESLRDGRWLDPVGIPGVYAALTADGTAIALLEEKGKRASPVFVVRPRGLVD

Sequences:

>Translated_306_residues
MGERSAPTRRVDPLGGLLVVDKDGGMTSHDVVARCRKILGTRKIGHAGTLDPMATGVLVLGVERATKLLGLLTLTTKAYT
ATIRLGSATTTDDAEGEVLTTVPAGHLGDAEVAAGVAALTGDIQQVPATVSAIKIGGERAYARHRAGEQVELAARPVTVS
RFEVLARRDVAGFVDLDVVVECSSGTYVRALARDLGAALGVGGHLTALRRTRVGPFTLDHARTLDALADEPRLNLDMDEA
VRIAFPHRAIDAREAESLRDGRWLDPVGIPGVYAALTADGTAIALLEEKGKRASPVFVVRPRGLVD
>Mature_305_residues
GERSAPTRRVDPLGGLLVVDKDGGMTSHDVVARCRKILGTRKIGHAGTLDPMATGVLVLGVERATKLLGLLTLTTKAYTA
TIRLGSATTTDDAEGEVLTTVPAGHLGDAEVAAGVAALTGDIQQVPATVSAIKIGGERAYARHRAGEQVELAARPVTVSR
FEVLARRDVAGFVDLDVVVECSSGTYVRALARDLGAALGVGGHLTALRRTRVGPFTLDHARTLDALADEPRLNLDMDEAV
RIAFPHRAIDAREAESLRDGRWLDPVGIPGVYAALTADGTAIALLEEKGKRASPVFVVRPRGLVD

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=226, Percent_Identity=37.1681415929204, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI4503337, Length=236, Percent_Identity=31.3559322033898, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI215599015, Length=236, Percent_Identity=31.3559322033898, Blast_Score=81, Evalue=1e-15,
Organism=Escherichia coli, GI2367200, Length=232, Percent_Identity=36.6379310344828, Blast_Score=149, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17553978, Length=200, Percent_Identity=29, Blast_Score=83, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323204, Length=217, Percent_Identity=31.7972350230415, Blast_Score=93, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6324037, Length=123, Percent_Identity=34.1463414634146, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI281364189, Length=200, Percent_Identity=32, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI281364187, Length=200, Percent_Identity=32, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI281364185, Length=200, Percent_Identity=32, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI281364183, Length=200, Percent_Identity=32, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI62471759, Length=200, Percent_Identity=32, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI17975520, Length=200, Percent_Identity=32, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_NOCFA (Q5YSE0)

Other databases:

- EMBL:   AP006618
- RefSeq:   YP_120265.1
- ProteinModelPortal:   Q5YSE0
- SMR:   Q5YSE0
- GeneID:   3111615
- GenomeReviews:   AP006618_GR
- KEGG:   nfa:nfa40530
- HOGENOM:   HBG397258
- OMA:   EYLGTIR
- ProtClustDB:   PRK03287
- BioCyc:   NFAR247156:NFA40530-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR015225
- InterPro:   IPR014780
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF09142 TruB_C; PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom; SSF88697 PUA-like

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 32184; Mature: 32053

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: NA

Important sites: ACT_SITE 51-51

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGERSAPTRRVDPLGGLLVVDKDGGMTSHDVVARCRKILGTRKIGHAGTLDPMATGVLVL
CCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHCEEEE
GVERATKLLGLLTLTTKAYTATIRLGSATTTDDAEGEVLTTVPAGHLGDAEVAAGVAALT
EHHHHHHHHHHHHEEEEEEEEEEEECCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHH
GDIQQVPATVSAIKIGGERAYARHRAGEQVELAARPVTVSRFEVLARRDVAGFVDLDVVV
CCHHHHCCEEEEEEECCHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEEEEE
ECSSGTYVRALARDLGAALGVGGHLTALRRTRVGPFTLDHARTLDALADEPRLNLDMDEA
ECCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCCEECHHHHHHHHHCCCCCCCCCHHHH
VRIAFPHRAIDAREAESLRDGRWLDPVGIPGVYAALTADGTAIALLEEKGKRASPVFVVR
EEEECCCHHCCHHHHHHHCCCCCCCCCCCCCEEEEEECCCCEEEEEECCCCCCCCEEEEC
PRGLVD
CCCCCC
>Mature Secondary Structure 
GERSAPTRRVDPLGGLLVVDKDGGMTSHDVVARCRKILGTRKIGHAGTLDPMATGVLVL
CCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHCEEEE
GVERATKLLGLLTLTTKAYTATIRLGSATTTDDAEGEVLTTVPAGHLGDAEVAAGVAALT
EHHHHHHHHHHHHEEEEEEEEEEEECCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHH
GDIQQVPATVSAIKIGGERAYARHRAGEQVELAARPVTVSRFEVLARRDVAGFVDLDVVV
CCHHHHCCEEEEEEECCHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEEEEE
ECSSGTYVRALARDLGAALGVGGHLTALRRTRVGPFTLDHARTLDALADEPRLNLDMDEA
ECCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCCEECHHHHHHHHHCCCCCCCCCHHHH
VRIAFPHRAIDAREAESLRDGRWLDPVGIPGVYAALTADGTAIALLEEKGKRASPVFVVR
EEEECCCHHCCHHHHHHHCCCCCCCCCCCCCEEEEEECCCCEEEEEECCCCCCCCEEEEC
PRGLVD
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA