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The map label for this gene is miaB [H]

Identifier: 54025804

GI number: 54025804

Start: 4054269

End: 4055015

Strand: Reverse

Name: miaB [H]

Synonym: nfa38340

Alternate gene names: 54025804

Gene position: 4055015-4054269 (Counterclockwise)

Preceding gene: 54025805

Following gene: 54025802

Centisome position: 67.35

GC content: 71.35

Gene sequence:

>747_bases
ATGACCGGGCCGGGCGAGCACGACGACGACCGCCGCGAGGGCGGCGCGGACGACGGCACCGATTCCGGTACTACGCCGCG
TAGTGGGTCGGCTGCCGGGGCGGAGCCCACGGATGGCACAGTGGACCGGGACCAATCCGGTGACGACTCAGGAGGCGAGG
ACGCGGTGAGCTCGGTCGAGCAGAGCAACGACTTCGATCAGTTCCGCGCGGAGATCGACGCGGTGGAGCGGCGGATCGCG
GGCGAGATCGATCCGGGCGTGCGTGCCATGGTGGTGGCGGGCGCGGTGTTCGTGCTGCTGCTGTCGCTGGTGCTCTCGCA
CGCGGGCAGCGCCCGCGGCTTCGACGTGCTGCTCGGCACCGACGCGGCCAGGATCGAGCACATCGGCCTGCCCTCGCGGG
TGTTCGTGTGGTTCGTCGCCGTGTTCGGCGTCGGCTTCTCGCTGCTGGCGTTGATGACCCGGCGCTGGGTGCTGGCCTGG
TTCGCGGTGGCGGGCTCGGCCATCGCGAGCGTGTTCGGCGTGCTGTCGATCTGGCACCGCCAGACCCCCGGCCTGGGCAA
CTACATGGGCGCGGGCCCCGGCATCGGCCTGGTGATCGGCACGATCGCCATCATCGTGCTGACCTTCCACTGGGTCCGCG
TGGTGTGGAGCCGCACGGCCTTGCACCTGGAAGCCGAGGAGCAGCGGCGCAACGCGGCCAAGGCCGCCGAGGAACGCGAC
CGCAAGCGCCTGACCGGCGAGGAGTGA

Upstream 100 bases:

>100_bases
CCCCGAAGACCGCGCCCATCGGTGTCGGTCTCGGCCTGCCCCGTATCGGCGCGCCCGCCCCCGAGCCGGTGGCCGCGGGC
TGCTCGACCGGTTGCGGCGC

Downstream 100 bases:

>100_bases
CCCCGACCGGCGACGCGTGCGCCGGCGCGGTCAGCGATTGCTGACCGCGCCCTCGGCGGCTTCGGCCCACTCCCGCCACT
GCTTGGCCTGCTCGAGCGCC

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 248; Mature: 247

Protein sequence:

>248_residues
MTGPGEHDDDRREGGADDGTDSGTTPRSGSAAGAEPTDGTVDRDQSGDDSGGEDAVSSVEQSNDFDQFRAEIDAVERRIA
GEIDPGVRAMVVAGAVFVLLLSLVLSHAGSARGFDVLLGTDAARIEHIGLPSRVFVWFVAVFGVGFSLLALMTRRWVLAW
FAVAGSAIASVFGVLSIWHRQTPGLGNYMGAGPGIGLVIGTIAIIVLTFHWVRVVWSRTALHLEAEEQRRNAAKAAEERD
RKRLTGEE

Sequences:

>Translated_248_residues
MTGPGEHDDDRREGGADDGTDSGTTPRSGSAAGAEPTDGTVDRDQSGDDSGGEDAVSSVEQSNDFDQFRAEIDAVERRIA
GEIDPGVRAMVVAGAVFVLLLSLVLSHAGSARGFDVLLGTDAARIEHIGLPSRVFVWFVAVFGVGFSLLALMTRRWVLAW
FAVAGSAIASVFGVLSIWHRQTPGLGNYMGAGPGIGLVIGTIAIIVLTFHWVRVVWSRTALHLEAEEQRRNAAKAAEERD
RKRLTGEE
>Mature_247_residues
TGPGEHDDDRREGGADDGTDSGTTPRSGSAAGAEPTDGTVDRDQSGDDSGGEDAVSSVEQSNDFDQFRAEIDAVERRIAG
EIDPGVRAMVVAGAVFVLLLSLVLSHAGSARGFDVLLGTDAARIEHIGLPSRVFVWFVAVFGVGFSLLALMTRRWVLAWF
AVAGSAIASVFGVLSIWHRQTPGLGNYMGAGPGIGLVIGTIAIIVLTFHWVRVVWSRTALHLEAEEQRRNAAKAAEERDR
KRLTGEE

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 64 in tRNAs that read codons beginning with uridine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 26399; Mature: 26268

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHCCCCCC
>Mature Secondary Structure 
TGPGEHDDDRREGGADDGTDSGTTPRSGSAAGAEPTDGTVDRDQSGDDSGGEDAVSSVE
CCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
QSNDFDQFRAEIDAVERRIAGEIDPGVRAMVVAGAVFVLLLSLVLSHAGSARGFDVLLGT
HCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECC
DAARIEHIGLPSRVFVWFVAVFGVGFSLLALMTRRWVLAWFAVAGSAIASVFGVLSIWHR
CHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QTPGLGNYMGAGPGIGLVIGTIAIIVLTFHWVRVVWSRTALHLEAEEQRRNAAKAAEERD
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHHHHHHHHHHHH
RKRLTGEE
HHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA