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The map label for this gene is miaB

Identifier: 54025805

GI number: 54025805

Start: 4055012

End: 4056523

Strand: Reverse

Name: miaB

Synonym: nfa38350

Alternate gene names: 54025805

Gene position: 4056523-4055012 (Counterclockwise)

Preceding gene: 54025810

Following gene: 54025804

Centisome position: 67.37

GC content: 70.83

Gene sequence:

>1512_bases
ATGACGCGAGCGCGCAGTTACGAGATCCGTACCTTCGGCTGCCAGATGAACGTGCACGATTCCGAACGTCTGTCCGGTCT
GCTCGAGGACGCCGGCTACGTCAAGGCCGCCCCGGGGGCGACGGCCGACCTGGTGGTGTTCAACACCTGCGCGGTCCGCG
AGAACGCCGACAACAAGCTCTACGGCACCCTGGGCCACCTCGCGCCGGTGAAGGCGGGCAGGCCGGGGATGCAGATCGCC
GTGGGCGGCTGTCTGGCCCAGAAGGACCGCGACACCGTGGTGCGCAAGGCGCCCTGGGTGGACGTGGTGTTCGGCACGCA
CAACATCGGCTCGCTGCCGGTGCTGCTCGAGCGCGCCCGCCACAACGAGCAGGCGCAGGTGGAGATCCTGGAGTCGCTGG
AGGCGTTCCCCTCGACGCTGCCCGCCAAGCGCGAGTCCGCCTACGCGGGCTGGGTGTCGATCTCGGTGGGCTGCAACAAC
ACCTGCACCTTCTGCATCGTGCCCGCGCTGCGCGGCAAGGAGGTCGACCGCAGGCCCGGTGACGTGCTCGCCGAGGTGCA
GGCCCTGGTCGATCAGGGCGTGCTCGAGGTGACGCTGCTCGGCCAGAACGTCAACTCCTACGGCGCGTCCTTCGCCGACC
CGGACGAGCCGCGCGACCGCGGCGCCTTCGCCAAGCTGTTGCGCGCGTGCGGCGGAATCGAGGGGCTGGAGCGGGTGCGC
TTCACCTCGCCGCATCCGGCGGAGTTCACCGACGACGTCATCGAGGCGATGGCGCAGACCCCCAACATCTGCCCGCAGCT
GCACATGCCGTTGCAGTCCGGCTCGGATCGGGTGCTCAAGGCGATGCGGCGCTCCTACCGTAAGGCGCGCTACCTGGGCA
TCATCGAGAAGGTGCGGGCGGCGATGCCGCACGCGGCCATCACCACCGACATCATCGTCGGCTTCCCCGGCGAGACCGAG
GAGGACTTCCAGGAGACCCTGGACGTGGTGCGCCAGGCCCGTTTCACCAGCGCGTTCACCTTCCAGTACTCCAAGCGGCC
CGGCACCCCGGCCGCGGAGATGGCCGACCAGGTGCCCAAGCAGGTGGTGCAGGAGCGCTACGACCGGCTGATCGCGTTGC
AGGAGGAGATCTCGCTGGCGGCCAACCGCGAGCTGATCGGCACCGAGGTGGAACTGTTGGTCGCCGAGGGCTCGGGCAAG
AAGAACGCCGCCACCGCGCGGATGAGCGGGCGCGCCAGGGACGGCAGGCTCGTGCACTTCCGGCCCGGCGGCACCGCCGA
GCCGATCCGGCCCGGCGACCTGGTCACCGTCGACATCACCGAGGCCGCGCCGCATCACCTGATCGCCGACGCGCCGGTCA
AGACGCATCGGCGCACCCGGGCGGGCGACGCGCACGAGCGCGGCATCACCCCGAAGACCGCGCCCATCGGTGTCGGTCTC
GGCCTGCCCCGTATCGGCGCGCCCGCCCCCGAGCCGGTGGCCGCGGGCTGCTCGACCGGTTGCGGCGCATGA

Upstream 100 bases:

>100_bases
GTGCCAGCGCGGATGCCGTGCGCGGGGCCCGCGGCGGCCCCCGTACCCTGGACAGGTGGAATCGACACCGAAGAGCATCG
GGGTGGACGCAGGACAGGTG

Downstream 100 bases:

>100_bases
CCGGGCCGGGCGAGCACGACGACGACCGCCGCGAGGGCGGCGCGGACGACGGCACCGATTCCGGTACTACGCCGCGTAGT
GGGTCGGCTGCCGGGGCGGA

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 503; Mature: 502

Protein sequence:

>503_residues
MTRARSYEIRTFGCQMNVHDSERLSGLLEDAGYVKAAPGATADLVVFNTCAVRENADNKLYGTLGHLAPVKAGRPGMQIA
VGGCLAQKDRDTVVRKAPWVDVVFGTHNIGSLPVLLERARHNEQAQVEILESLEAFPSTLPAKRESAYAGWVSISVGCNN
TCTFCIVPALRGKEVDRRPGDVLAEVQALVDQGVLEVTLLGQNVNSYGASFADPDEPRDRGAFAKLLRACGGIEGLERVR
FTSPHPAEFTDDVIEAMAQTPNICPQLHMPLQSGSDRVLKAMRRSYRKARYLGIIEKVRAAMPHAAITTDIIVGFPGETE
EDFQETLDVVRQARFTSAFTFQYSKRPGTPAAEMADQVPKQVVQERYDRLIALQEEISLAANRELIGTEVELLVAEGSGK
KNAATARMSGRARDGRLVHFRPGGTAEPIRPGDLVTVDITEAAPHHLIADAPVKTHRRTRAGDAHERGITPKTAPIGVGL
GLPRIGAPAPEPVAAGCSTGCGA

Sequences:

>Translated_503_residues
MTRARSYEIRTFGCQMNVHDSERLSGLLEDAGYVKAAPGATADLVVFNTCAVRENADNKLYGTLGHLAPVKAGRPGMQIA
VGGCLAQKDRDTVVRKAPWVDVVFGTHNIGSLPVLLERARHNEQAQVEILESLEAFPSTLPAKRESAYAGWVSISVGCNN
TCTFCIVPALRGKEVDRRPGDVLAEVQALVDQGVLEVTLLGQNVNSYGASFADPDEPRDRGAFAKLLRACGGIEGLERVR
FTSPHPAEFTDDVIEAMAQTPNICPQLHMPLQSGSDRVLKAMRRSYRKARYLGIIEKVRAAMPHAAITTDIIVGFPGETE
EDFQETLDVVRQARFTSAFTFQYSKRPGTPAAEMADQVPKQVVQERYDRLIALQEEISLAANRELIGTEVELLVAEGSGK
KNAATARMSGRARDGRLVHFRPGGTAEPIRPGDLVTVDITEAAPHHLIADAPVKTHRRTRAGDAHERGITPKTAPIGVGL
GLPRIGAPAPEPVAAGCSTGCGA
>Mature_502_residues
TRARSYEIRTFGCQMNVHDSERLSGLLEDAGYVKAAPGATADLVVFNTCAVRENADNKLYGTLGHLAPVKAGRPGMQIAV
GGCLAQKDRDTVVRKAPWVDVVFGTHNIGSLPVLLERARHNEQAQVEILESLEAFPSTLPAKRESAYAGWVSISVGCNNT
CTFCIVPALRGKEVDRRPGDVLAEVQALVDQGVLEVTLLGQNVNSYGASFADPDEPRDRGAFAKLLRACGGIEGLERVRF
TSPHPAEFTDDVIEAMAQTPNICPQLHMPLQSGSDRVLKAMRRSYRKARYLGIIEKVRAAMPHAAITTDIIVGFPGETEE
DFQETLDVVRQARFTSAFTFQYSKRPGTPAAEMADQVPKQVVQERYDRLIALQEEISLAANRELIGTEVELLVAEGSGKK
NAATARMSGRARDGRLVHFRPGGTAEPIRPGDLVTVDITEAAPHHLIADAPVKTHRRTRAGDAHERGITPKTAPIGVGLG
LPRIGAPAPEPVAAGCSTGCGA

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=481, Percent_Identity=34.9272349272349, Blast_Score=290, Evalue=3e-78,
Organism=Homo sapiens, GI28872782, Length=477, Percent_Identity=34.8008385744235, Blast_Score=290, Evalue=3e-78,
Organism=Homo sapiens, GI93277076, Length=471, Percent_Identity=29.2993630573248, Blast_Score=162, Evalue=1e-39,
Organism=Escherichia coli, GI1786882, Length=449, Percent_Identity=40.7572383073497, Blast_Score=340, Evalue=1e-94,
Organism=Escherichia coli, GI1787057, Length=450, Percent_Identity=31.7777777777778, Blast_Score=202, Evalue=4e-53,
Organism=Caenorhabditis elegans, GI17553146, Length=461, Percent_Identity=34.70715835141, Blast_Score=253, Evalue=2e-67,
Organism=Caenorhabditis elegans, GI71996771, Length=224, Percent_Identity=33.9285714285714, Blast_Score=121, Evalue=9e-28,
Organism=Drosophila melanogaster, GI21356207, Length=424, Percent_Identity=36.7924528301887, Blast_Score=290, Evalue=1e-78,
Organism=Drosophila melanogaster, GI19922432, Length=480, Percent_Identity=29.1666666666667, Blast_Score=166, Evalue=3e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_NOCFA (Q5YT08)

Other databases:

- EMBL:   AP006618
- RefSeq:   YP_120047.1
- ProteinModelPortal:   Q5YT08
- SMR:   Q5YT08
- GeneID:   3107100
- GenomeReviews:   AP006618_GR
- KEGG:   nfa:nfa38350
- NMPDR:   fig|247156.1.peg.3736
- HOGENOM:   HBG457663
- OMA:   ANLHDDT
- ProtClustDB:   PRK14326
- BioCyc:   NFAR247156:NFA38350-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 54189; Mature: 54058

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRARSYEIRTFGCQMNVHDSERLSGLLEDAGYVKAAPGATADLVVFNTCAVRENADNKL
CCCCCCEEEEEEEEEECCCCHHHHHHHHHCCCCEEECCCCCEEEEEEEEEEECCCCCCCC
YGTLGHLAPVKAGRPGMQIAVGGCLAQKDRDTVVRKAPWVDVVFGTHNIGSLPVLLERAR
EEHHHHHCCCCCCCCCCEEEECCHHCCCCHHHHHHCCCCEEEEECCCCCCCHHHHHHHHH
HNEQAQVEILESLEAFPSTLPAKRESAYAGWVSISVGCNNTCTFCIVPALRGKEVDRRPG
CCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEECCCCCEEEEEEECCCCCCCCCCCH
DVLAEVQALVDQGVLEVTLLGQNVNSYGASFADPDEPRDRGAFAKLLRACGGIEGLERVR
HHHHHHHHHHHCCCEEEEEECCCHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHCC
FTSPHPAEFTDDVIEAMAQTPNICPQLHMPLQSGSDRVLKAMRRSYRKARYLGIIEKVRA
CCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AMPHAAITTDIIVGFPGETEEDFQETLDVVRQARFTSAFTFQYSKRPGTPAAEMADQVPK
HCCCHHEEEEEEEECCCCCHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCHHHHHHHHHH
QVVQERYDRLIALQEEISLAANRELIGTEVELLVAEGSGKKNAATARMSGRARDGRLVHF
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHHHCCCCCCCCEEEE
RPGGTAEPIRPGDLVTVDITEAAPHHLIADAPVKTHRRTRAGDAHERGITPKTAPIGVGL
CCCCCCCCCCCCCEEEEEECCCCCCCEEECCCHHHHHHCCCCCHHHCCCCCCCCCEECCC
GLPRIGAPAPEPVAAGCSTGCGA
CCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TRARSYEIRTFGCQMNVHDSERLSGLLEDAGYVKAAPGATADLVVFNTCAVRENADNKL
CCCCCEEEEEEEEEECCCCHHHHHHHHHCCCCEEECCCCCEEEEEEEEEEECCCCCCCC
YGTLGHLAPVKAGRPGMQIAVGGCLAQKDRDTVVRKAPWVDVVFGTHNIGSLPVLLERAR
EEHHHHHCCCCCCCCCCEEEECCHHCCCCHHHHHHCCCCEEEEECCCCCCCHHHHHHHHH
HNEQAQVEILESLEAFPSTLPAKRESAYAGWVSISVGCNNTCTFCIVPALRGKEVDRRPG
CCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEECCCCCEEEEEEECCCCCCCCCCCH
DVLAEVQALVDQGVLEVTLLGQNVNSYGASFADPDEPRDRGAFAKLLRACGGIEGLERVR
HHHHHHHHHHHCCCEEEEEECCCHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHCC
FTSPHPAEFTDDVIEAMAQTPNICPQLHMPLQSGSDRVLKAMRRSYRKARYLGIIEKVRA
CCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AMPHAAITTDIIVGFPGETEEDFQETLDVVRQARFTSAFTFQYSKRPGTPAAEMADQVPK
HCCCHHEEEEEEEECCCCCHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCHHHHHHHHHH
QVVQERYDRLIALQEEISLAANRELIGTEVELLVAEGSGKKNAATARMSGRARDGRLVHF
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHHHCCCCCCCCEEEE
RPGGTAEPIRPGDLVTVDITEAAPHHLIADAPVKTHRRTRAGDAHERGITPKTAPIGVGL
CCCCCCCCCCCCCEEEEEECCCCCCCEEECCCHHHHHHCCCCCHHHCCCCCCCCCEECCC
GLPRIGAPAPEPVAAGCSTGCGA
CCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA