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The map label for this gene is gatA

Identifier: 54019988

GI number: 54019988

Start: 34903

End: 36222

Strand: Direct

Name: gatA

Synonym: mhp029

Alternate gene names: 54019988

Gene position: 34903-36222 (Clockwise)

Preceding gene: 54019987

Following gene: 54019989

Centisome position: 3.91

GC content: 30.0

Gene sequence:

>1320_bases
ATGACTAAATTTCAGCTTAGTTTTCAAGAAGCACTTGAGAAATTAAAAAATGATAAAAATAATACGGTTGCTTACTTTTT
TCAAGAAAAAAACAACAAAAAAGGTGTTTTGGCAAATAAATTTTTTAGCATCAAATCGAATTTTGCAACAACTGAAGGTG
TTTCTCATGCTTCTTCAATTTCCCTTTTAAATTTTAAACCCTCTTATAATGCAACTGTTTTTCAAAAATTAATTAATGCG
GGCGCACAACCACTTGTAAAAGTTCATAATGACGAGTTGGGTTTAGGTGGAAAAGGTCTTTTTTCAGCTTTCGGTAAAAT
TGTAAATCCTTTAGACAAAACAAAATTAATTGGCGGATCTTCATCTGGAAGTGCCGCAACAATTAATTTTGTTCACTTCG
CAATTGGCTCAGACACCGGCGATTCGATTAGAAGGCCGGCTAGTTTTATCGGGAAAGTTGGATTTAAACCTTCATATGGT
GCTGTATCCCGTTATGGATTGTTTCCTTATGCCACTTCGTTGGATACAGTAGCGTGGTTAACTCATAATGTTCTGGATTC
TATTTTAGTATCACAGGTAGTTTTTGGTTCTGATGAAAACGACTTAACATCTGTTGAAATCGATGTTAAAAATGTTGAAA
AAACAAAACCAAAAAAAGTTGCATTTCTTGATTTGGATGCTGAAATTGAAGATTATGTAGTAAAAAAACTCTATAAATTA
CGGAATATTTTAAAATCTGACCAGGTAGAAATAGAGATAATTAAACCAGATTTAAACCTATTAAAAACAATTCTACCTGT
TTATGAAATAATTTCTTATTCAGAGGCAACCTCAAATTTATCCGCAATTAATGGGATAACTTTTGGGAATACTGAAAAAA
ATTTAAAATGAGAAGAGATTTTTATTAGAACAAGATCAGATGGTTTTGGTTTGATGCTGCAAAAAAGACTAATTTGAGGC
TCTTTTTTTCTTGAAAAAGATAATCAAGAGCATTTTTTTATTAAAGCCAAAAAAATGAGAACTTTAATTAAGAATTATTA
TGAATCTATACTGAATAAATTTGATTTAGTAGTTTTTCCTGCTTTTTATGGCATTGCCCCTGATATTCAAGGTGAAAACA
GTGAAAAAAGTGATAAAATTACTAATTTTATTCTAGCTATTTCGAATTTAGTTGGCAATCCATCAATTAGTTTGCCATTA
GGTAGTTATAAAAATTTGCCTTTTAATTTAGCGGTTGATTCAAAAATAAATTCTGATGCAGAATTGTTAGGTTTTTCCCT
TTATCTTGAAGAAAAAATAAGCGAAATTAATAATGAATAA

Upstream 100 bases:

>100_bases
TTATTTGAGGATATTCCAGATTTTACCAGTTTTAGAGAACAACTTTTTGACAATTCTATTCACAACAGTGAAAAGCAAAT
TATAATTAAAAAGGTAATTG

Downstream 100 bases:

>100_bases
TAAATATTTAGTCACAATTGGGATTGAAATTCATTTGGAATTAAATACAAAAGCAAAAATGTTTTCAAATTCACCGAATT
TACGCGGTGAAGCAAATCAA

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 439; Mature: 438

Protein sequence:

>439_residues
MTKFQLSFQEALEKLKNDKNNTVAYFFQEKNNKKGVLANKFFSIKSNFATTEGVSHASSISLLNFKPSYNATVFQKLINA
GAQPLVKVHNDELGLGGKGLFSAFGKIVNPLDKTKLIGGSSSGSAATINFVHFAIGSDTGDSIRRPASFIGKVGFKPSYG
AVSRYGLFPYATSLDTVAWLTHNVLDSILVSQVVFGSDENDLTSVEIDVKNVEKTKPKKVAFLDLDAEIEDYVVKKLYKL
RNILKSDQVEIEIIKPDLNLLKTILPVYEIISYSEATSNLSAINGITFGNTEKNLKWEEIFIRTRSDGFGLMLQKRLIWG
SFFLEKDNQEHFFIKAKKMRTLIKNYYESILNKFDLVVFPAFYGIAPDIQGENSEKSDKITNFILAISNLVGNPSISLPL
GSYKNLPFNLAVDSKINSDAELLGFSLYLEEKISEINNE

Sequences:

>Translated_439_residues
MTKFQLSFQEALEKLKNDKNNTVAYFFQEKNNKKGVLANKFFSIKSNFATTEGVSHASSISLLNFKPSYNATVFQKLINA
GAQPLVKVHNDELGLGGKGLFSAFGKIVNPLDKTKLIGGSSSGSAATINFVHFAIGSDTGDSIRRPASFIGKVGFKPSYG
AVSRYGLFPYATSLDTVAWLTHNVLDSILVSQVVFGSDENDLTSVEIDVKNVEKTKPKKVAFLDLDAEIEDYVVKKLYKL
RNILKSDQVEIEIIKPDLNLLKTILPVYEIISYSEATSNLSAINGITFGNTEKNLK*EEIFIRTRSDGFGLMLQKRLI*G
SFFLEKDNQEHFFIKAKKMRTLIKNYYESILNKFDLVVFPAFYGIAPDIQGENSEKSDKITNFILAISNLVGNPSISLPL
GSYKNLPFNLAVDSKINSDAELLGFSLYLEEKISEINNE
>Mature_438_residues
TKFQLSFQEALEKLKNDKNNTVAYFFQEKNNKKGVLANKFFSIKSNFATTEGVSHASSISLLNFKPSYNATVFQKLINAG
AQPLVKVHNDELGLGGKGLFSAFGKIVNPLDKTKLIGGSSSGSAATINFVHFAIGSDTGDSIRRPASFIGKVGFKPSYGA
VSRYGLFPYATSLDTVAWLTHNVLDSILVSQVVFGSDENDLTSVEIDVKNVEKTKPKKVAFLDLDAEIEDYVVKKLYKLR
NILKSDQVEIEIIKPDLNLLKTILPVYEIISYSEATSNLSAINGITFGNTEKNLK*EEIFIRTRSDGFGLMLQKRLI*GS
FFLEKDNQEHFFIKAKKMRTLIKNYYESILNKFDLVVFPAFYGIAPDIQGENSEKSDKITNFILAISNLVGNPSISLPLG
SYKNLPFNLAVDSKINSDAELLGFSLYLEEKISEINNE

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=433, Percent_Identity=28.6374133949192, Blast_Score=162, Evalue=7e-40,
Organism=Homo sapiens, GI195972892, Length=177, Percent_Identity=32.2033898305085, Blast_Score=68, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17543272, Length=397, Percent_Identity=28.2115869017632, Blast_Score=149, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI71990152, Length=405, Percent_Identity=24.6913580246914, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323950, Length=463, Percent_Identity=29.5896328293737, Blast_Score=157, Evalue=4e-39,
Organism=Drosophila melanogaster, GI24648113, Length=425, Percent_Identity=28.9411764705882, Blast_Score=162, Evalue=4e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 48501; Mature: 48370

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: PS00012 PHOSPHOPANTETHEINE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKFQLSFQEALEKLKNDKNNTVAYFFQEKNNKKGVLANKFFSIKSNFATTEGVSHASSI
CCEEECHHHHHHHHHHCCCCCEEEEEEEECCCCCCEEHHHHHHHHCCCCCCCCCCCCCCE
SLLNFKPSYNATVFQKLINAGAQPLVKVHNDELGLGGKGLFSAFGKIVNPLDKTKLIGGS
EEEEECCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHCCHHHHEEECCC
SSGSAATINFVHFAIGSDTGDSIRRPASFIGKVGFKPSYGAVSRYGLFPYATSLDTVAWL
CCCCEEEEEEEEEEECCCCCHHHHCCHHHHHCCCCCCCCCCHHHCCCCCCCCCHHHHHHH
THNVLDSILVSQVVFGSDENDLTSVEIDVKNVEKTKPKKVAFLDLDAEIEDYVVKKLYKL
HHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHH
RNILKSDQVEIEIIKPDLNLLKTILPVYEIISYSEATSNLSAINGITFGNTEKNLKEEIF
HHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCEEE
IRTRSDGFGLMLQKRLIGSFFLEKDNQEHFFIKAKKMRTLIKNYYESILNKFDLVVFPAF
EEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCEEEEHHH
YGIAPDIQGENSEKSDKITNFILAISNLVGNPSISLPLGSYKNLPFNLAVDSKINSDAEL
HCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCEEEEECCCCCCCHHH
LGFSLYLEEKISEINNE
HHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKFQLSFQEALEKLKNDKNNTVAYFFQEKNNKKGVLANKFFSIKSNFATTEGVSHASSI
CEEECHHHHHHHHHHCCCCCEEEEEEEECCCCCCEEHHHHHHHHCCCCCCCCCCCCCCE
SLLNFKPSYNATVFQKLINAGAQPLVKVHNDELGLGGKGLFSAFGKIVNPLDKTKLIGGS
EEEEECCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHCCHHHHEEECCC
SSGSAATINFVHFAIGSDTGDSIRRPASFIGKVGFKPSYGAVSRYGLFPYATSLDTVAWL
CCCCEEEEEEEEEEECCCCCHHHHCCHHHHHCCCCCCCCCCHHHCCCCCCCCCHHHHHHH
THNVLDSILVSQVVFGSDENDLTSVEIDVKNVEKTKPKKVAFLDLDAEIEDYVVKKLYKL
HHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHH
RNILKSDQVEIEIIKPDLNLLKTILPVYEIISYSEATSNLSAINGITFGNTEKNLKEEIF
HHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCEEE
IRTRSDGFGLMLQKRLIGSFFLEKDNQEHFFIKAKKMRTLIKNYYESILNKFDLVVFPAF
EEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCEEEEHHH
YGIAPDIQGENSEKSDKITNFILAISNLVGNPSISLPLGSYKNLPFNLAVDSKINSDAEL
HCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCEEEEECCCCCCCHHH
LGFSLYLEEKISEINNE
HHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]